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Citations for Bio-Rad :
151 - 200 of 222 citations for RPL3L Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The membranes were incubated with 5% Blocking-grade blocker non-fat milk (Cat no. 1706404, Bio-Rad) plus 0.5% Bovine Serum Albumin (BSA ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated shaking for 1 hour at RT with 4% blocking solution (5% milk powder (BioRad) in TBS-T) ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated in blocking buffer containing horseradish peroxidase-conjugated secondary antibodies at 1:10,000 dilution (Bio-Rad #172-1011 or 172-1019 ...
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bioRxiv - Immunology 2022Quote: Excess Eα peptide was removed with Bio-spin 30 kDa size exclusion columns (Bio-Rad Laboratories) equilibrated with PBS according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Immunoblot detection of 6xHIS and 2A peptide tags were determined through anti-Histidine tag (AD1.1.10, Biorad) and anti-2A peptide (3H4 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the membrane blots were blocked in 5% blocking buffer (5% non-fat dry milk, Bio-Rad, in TBST) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... After blocking for 45 minutes at room temperature with 10% non-fat milk (Blotting-Grade Blocker, Bio-Rad) in TBS containing 0.01% Tween 20 (TBS-T) ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were incubated overnight at 4°C in blocking solution 5% (w/v) Blotting-Grade Blocker (BioRad) in PBS-Tween buffer (1× PBS ...
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bioRxiv - Immunology 2021Quote: ... diluted 1:15000 in blocking buffer) followed by a goat anti-rabbit-HRP conjugate (170-6515, Bio-Rad, Hercules ...
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bioRxiv - Genetics 2019Quote: ... and the PVDF membrane was blocked in 5% non-fat dry milk-based blocking-grade blocker (Bio-Rad) for 1 hr at room temperature ...
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bioRxiv - Physiology 2023Quote: ... membranes were housed in clear blotting boxes and blocked in 5% blocking buffer in TBS-T (BIORAD 1706404) for 40 min at +25°C ...
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bioRxiv - Genomics 2024Quote: ... TBST (EZ BioResearch #S-1012, diluted to 1X in deionized water) and EveryBlot Blocking Buffer (Bio-Rad #12010020) were used for washing and blocking ...
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bioRxiv - Cell Biology 2024Quote: ... The slides were covered in a blocking solution made up of in Tris-buffered saline (#1706435; Bio-Rad) with 0.1% (vol/vol ...
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bioRxiv - Plant Biology 2020Quote: ... Peptide concentration was determined by a modified Lowry procedure using the DC Protein Assay (BioRad; Munich, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: Electrophoresis was used to separate peptides in a 16.5% Mini-PROTEAN® Tris-Tricine Gel (Bio-Rad) with tris-tricine (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... 20 mL blocking buffer supplemented with 5 μL of horseradish peroxidase (HRP) conjugated rabbit anti-mouse secondary antibody (BioRad) was added and incubated for 2 hours at room temperature with agitation ...
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bioRxiv - Molecular Biology 2021Quote: ... the Netherlands) and blocked for 1 hour at room temperature using Odyssey blocking buffer (LI-COR biosciences, Bio-Rad) in a 1:1 dilution with tris-buffered saline (TBS ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed (previously described) and 100 μL of diluted mouse serum (PBST wash buffer, 0.1% Biorad Blocking Agent) was added in duplicate and incubated for one hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... endogenous peroxidase activity was blocked in 3.5% (v/v) hydrogen peroxide and blocking with 10% donkey serum (Bio-Rad) was performed ...
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bioRxiv - Cell Biology 2023Quote: ... endogenous peroxidase activity was blocked in 3.5% (v/v) hydrogen peroxide and blocking with 10% donkey serum (Bio-Rad) was performed ...
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bioRxiv - Cell Biology 2022Quote: ... The transferred membrane was briefly washed with TBST (0.1% Tween-20 in TBS) and blocked with Blocking Buffer (5% Blotting-Grade Blocker [1706404, BioRad] or 5% BSA [A9647-50G ...
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bioRxiv - Cancer Biology 2022Quote: ... Supernatants were transferred to new tubes and peptide concentrations were quantified using the DC Protein Assay (Bio-Rad). Peptides were labeled using 10-plex TMT reagents as described above and 4 μg of proteins were used for each TMT channel ...
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bioRxiv - Cancer Biology 2022Quote: ... Supernatants were transferred to new tubes and peptide concentrations were quantified using the DC Protein Assay (Bio-Rad). 50 μg of each sample were set aside for TMT labeling ...
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bioRxiv - Biochemistry 2021Quote: ... The membrane was let dry and thereafter blocked on a shaker for 10 min in 15 ml blocking buffer (1% casein in TBS, BioRad). The blocking solution was discarded ...
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bioRxiv - Neuroscience 2022Quote: ... The blots were then treated with HRP-conjugated goat anti-moue secondary antibody (1:10000) diluted in blocking solution for 1 h and developed with enhanced chemiluminescent (ECL) substrate (Cat#1705060, Biorad).
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bioRxiv - Genomics 2020Quote: ... Membranes were washed using tris-based saline buffer solutions with 0.1% tween-20 (TBST) and blocking solutions were prepared using 5% w/v western-blot grade dry milk powder (Biorad). Membranes were blocked with blocking buffer for 1 hour and washed between incubations for 5-10 mins (3x) ...
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bioRxiv - Immunology 2022Quote: ... Primary antibodies (for further specification see key resource table, table 1) were diluted in blocking solution (F4/80 [Bio-Rad] ...
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bioRxiv - Pathology 2020Quote: ... both used at 1:1,000 dilution in blocking buffer) and fluorescent signals were acquired using with a GelDoc Imager (Bio-Rad).
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bioRxiv - Immunology 2020Quote: ... Immunoblotting was performed according to standard protocols with initial blocking in PBS with 10% w/v clotting grade blocker (BioRad) or 3% w/v BSA (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were stripped using Restore™ Western Blot Stripping Buffer (Cat. number: 21059) following the manufacturer’s protocol and blocked with 5% Blocking-Grade Blocker solution (Bio-Rad) for 2 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... 20% DMSO and 0.3M Glycine for 1h followed by a blocking step using an aqueous solution of 0.2% Triton X100 (Bio-Rad Laboratories), 0.2M Tris pH8 ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was cut lengthwise into strips to isolate bands of particular molecular weights before being washed with TBST and incubated at room temperature for 1 H in a 5% blocking buffer solution (Biorad). The western blot strips were then incubated overnight at 4 C in a solution consisting of the primary antibody diluted into blocking buffer ...
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bioRxiv - Genetics 2023Quote: ... Blots were washed in 1x TBST with mild agitation before incubation with horseradish peroxidase-conjugated secondary antibodies diluted in blocking buffer (α- mouse 1:10,000 (170-6516; BioRad), α-rabbit 1:10,000 (170-6515 ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were stained with primary antibody in blocking buffer at 4°C overnight: anti-NP (1:5000, OBT1555, Bio-Rad), anti-PA ...
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bioRxiv - Cell Biology 2021Quote: ... The N-ter (pI 5.5) and C-ter (pI 9.9) peptides were conjugated to Affi-Gel-10 (Bio-rad) agarose beads for affinity-purification ...
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bioRxiv - Cell Biology 2022Quote: ... 1.2 μl of the resuspended peptide was spotted on to a nitrocellulose membrane (BIO-RAD, 0.2 μm #162-0112) and left to air-dry ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated overnight at 4 °C with the following primary antibodies (1:100, in blocking buffer): mouse anti-human MBP (BMK-13, antihuman MBP (BMK-13, MCA5751 Bio-RAD), rabbit anti-human PD-L1 (E1L3N ...
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bioRxiv - Immunology 2021Quote: ... Each well was then coated with 150 µl of blocking buffer consisting of PBS and 40 mg/ml blotting-grade blocker (Bio-Rad). The plates were incubated with blocking buffer for 1 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... were treated with 20% blocking buffer (Li-Cor Biosciences, Lincoln, US) followed by incubation with anti-His-tag mouse primary antibody (dilution1/5000, Bio-Rad) and revelation with a IRDye 800CW Goat anti-mouse IgG secondary antibody (dilution 1/10000 ...
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bioRxiv - Pathology 2021Quote: ... The sections were then incubated in blocking buffer (10% goat serum; 1 hour) followed by incubation with either rat anti-F4/80 antibody (Bio-Rad) diluted 1:500 or rat anti-CD68 antibody (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... Each well was then coated with 150 µl of a blocking buffer consisting of PBS and 40 mg ml-1 blotting-grade blocker (Bio-Rad). The plates were incubated with the blocking buffer for 1 h at room temperature ...
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bioRxiv - Immunology 2019Quote: ... NK cells were pre-incubated with activating IB4 antibody (IgG2a, gift from Dr. Fabio Malavasi, University of Torino) blocking CD38 (clone AT13/5, Bio-Rad), CD31 (clone WM59 ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were blocked 1 hr at room temperature with Odyssey PBS Blocking Buffer (Li-Cor, 927-40000) or in 5% Blotting-Grade Blocker (Biorad, 1706404) in TBST (50 mM Tris buffer ...
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bioRxiv - Genetics 2020Quote: ... membranes were washed in × 1 Tris-buffered saline-Tween (TSBT) and then blocked for ~10 min in 5% blocking buffer (Bio-Rad) in 1 × TBST ...
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bioRxiv - Microbiology 2021Quote: ... Each well was then coated with 150 µl of a blocking buffer consisting of PBS and 40 mg ml-1 blotting-grade blocker (Bio-Rad). The plates were incubated with the blocking buffer for 1 hour at room temperature ...
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bioRxiv - Immunology 2022Quote: ... then blocked for 1 to 3 hr at RT with 50 μL/well of blocking buffer (HN: 5% Blotting Grade Blocker, Bio-Rad cat ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was blocked by incubating in 15 mL of blocking solution (5% Blotting Grade Blocker Non-Fat Dry Milk (Bio-Rad) in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were transferred onto Immun-Blot PVDF membrane (BioRab, 1620177) and incubated overnight in blocking solution (5% powdered milk, NaF in TBST [TBS (BioRad, 1706435) 1% Tween] ...
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bioRxiv - Immunology 2022Quote: ... Blocking buffer was removed and 100 µL/well of samples and IFN-γ standards (recombinant bovine IFN-γ; Bio-Rad Antibodies) starting at 300 ng/mL followed by two-fold dilutions added ...
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bioRxiv - Cancer Biology 2023Quote: ... After blocking with 5% (w/v) BSA or milk the membrane was incubated with the corresponding primary and secondary antibodies (Bio-Rad). The specific bands were analyzed using Thyphoon or ChemiDoc Imaging Systems (Bio-Rad).