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Citations for Bio-Rad :
51 - 100 of 274 citations for Plasma kallikrein KLKB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: A multiplex biometric immunoassay containing fluorescent dyed microbeads was used to measure cytokines in plasma samples (Bio-Rad Laboratories). The following cytokines were quantified ...
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bioRxiv - Immunology 2023Quote: IL-27 plasma levels were assessed using the Mouse Cytokine 23-Plex immunoassay (Bio-Rad Laboratories, Inc., CA, USA) and by the IL-27 Mouse ELISA kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Lavage fluid and plasma cytokine quantities were measured by Bioplex Pro mouse cytokine 23-plex assay (Bio-Rad #M60009RDPD) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... rat anti-human CD8 (Bio-Rad), rat anti-human Granzyme B (eBioscience) ...
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bioRxiv - Genetics 2023Quote: ... Human (Bio-Rad, 10031243, ID: dHsaCP1000002). Reference assay for 3.1 kb deletion and 3.1 kb inversion assays ...
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bioRxiv - Neuroscience 2023Quote: ... anti-human CX3CR1 (Bio-Rad, AHP566). Mouse TNFα ELISA Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-human CX3CR1 (Biorad, AHP1589), mouse anti-human CD68 (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... and then incubated for at least 16 hours at 4°C with either mouse monoclonal anti-His (1:2,000; AD1.1.10, Biorad) or mouse anti-RNA pol (1:5,000 ...
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bioRxiv - Biochemistry 2022Quote: ... and the protein was passed through Hi-Q anion exchange column (Bio-Rad Laboratories, Hercules, CA, USA) to remove minor nucleic acid contamination ...
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bioRxiv - Plant Biology 2021Quote: Purified His and GST fusion proteins or GST alone (500 ng) were blotted onto nitrocellulose membrane (BioRad). The nitrocellulose membrane was rinsed with TBS-T buffer (10 mM Tris-HCl at pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... Eluents were collected and incubated with 50μL of packed Ni-NTA resin (Bio-Rad His-Pur 88222) at room temperature with nutating for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... and cultures were induced with IPTG and His-MBP-ORC2 was purified on Ni-NTA beads (BioRad). Purified protein was injected into rabbits for serum generation and collection (Cocalico Biologicals) ...
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bioRxiv - Biophysics 2022Quote: ... His-tagged σs was added to phosphorylated/unphosphorylated RssB in Micro Bio-Spin Chromatography columns (Bio-Rad) before removing 6μL of the input for SDS-PAGE analysis and adding 40μL of prepared HisPur Ni-NTA resin (Thermo Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... and human proinflammatory cytokines were quantified using the Bio-Plex Pro™ Human Inflammation Panel 1 (BioRad). Fluorescence was read using a MAGPIX System (Luminex ...
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bioRxiv - Biochemistry 2020Quote: ... cell lysates (50 - 100 μg) or plasma membrane fractions (80 - 100 μg) were resolved on SDS-PAGE gel and transferred to PVDF membrane (BIORAD). The membranes were blocked with 5% BSA and immunoblotted using primary anti-phospho-β2AR antibody (1:1000 ...
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bioRxiv - Microbiology 2019Quote: Aliquots of extracted nucleic acid from plasma were processed in triplicate (1.5ul per replicate) following the recommended workflow (WX200 ddPCR system, Bio-Rad). Custom PrimeTime (IDT ...
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bioRxiv - Biochemistry 2021Quote: The 100µl plasma was inactivated by heating at 56°C for 30 min 10 .60µl plasma was used for albumin and globulin depletion using an Aurum serum mini kit (BioRad, USA). The six depleted plasma samples were used for SWATH-MS analysis ...
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bioRxiv - Neuroscience 2022Quote: ... Peripheral plasma cytokine levels were determined using Bio-Rad Pro mouse cytokine multiplex commercial kits (Bio-Rad, Montreal, QC, Canada). Also ...
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bioRxiv - Immunology 2023Quote: The previously obtained plasma (1 µl) and gut mucus (30 µl) were separated on a 4-15% Mini-PROTEAN TGX gel (BioRad) under non-reducing conditions ...
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ANGPTL8 R59W variant influences inflammation through modulating NF-κB pathway under TNFα stimulationbioRxiv - Biochemistry 2023Quote: ... and the plasma levels of the various analytes were determined using the Bio-plex 200 system (Bio-Rad, CA, USA). The concentrations of the unknown samples were calculated by the Bio-plex Manager software (v 6.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasma vitamin B12 concentrations were assessed using the BioRad Quantaphase II radio assay (Hercules, CA, USA;74. Plasma folate concentrations were also measured using the BioRad Quantaphase II radio assay (Hercules ...
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bioRxiv - Immunology 2023Quote: Circulating plasma levels of cytokines and chemokines were measured using the Luminex MAGPIX Multiplex Assay system (Bio-Rad, Hercules, CA). Luminex Human Magnetic Assay Kit 45 Plex (R&D Systems ...
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bioRxiv - Microbiology 2020Quote: ... was used to purify the C-terminal 6X-His EfgA with 1 mL Ni-NTA columns (Bio-Rad). Columns were equilibrated with 10 mL of Buffer A at 1 mL/min prior to loading lysates ...
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bioRxiv - Biochemistry 2022Quote: ... and eluted from the C4 reverse-phase column (Hi-Pore reversed-phase column, 4.6 × 250 mm, Bio-Rad) by the acidic acetonitrile gradient.
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bioRxiv - Microbiology 2023Quote: ... His-tagged proteins in the cleared lysate were purified using Profinity™ IMAC Nickel Charged Resin (Bio-Rad) and eluted in 50 mM sodium phosphate buffer with 300 mM NaCl pH 8 and 300 mM imidazole (Fischer Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... monoclonal rat anti-human CD3 (Bio-Rad), rat anti-mouse B220/CD45R (clone RA3-6B2 ...
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bioRxiv - Immunology 2023Quote: ... goat-anti-human 1:3000 (Biorad /1721050); goat-anti-rabbit 1:2000 (CST / 7074) ...
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bioRxiv - Microbiology 2022Quote: ... Plasma viral load was measured using qRT-PCR on a C1000 Thermal Cycler and the CFX96 Real-Time system (Bio-Rad) with 100 copies/ml limit of detection.
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bioRxiv - Bioengineering 2021Quote: ... Membranes were stained overnight at 4°C with a 1:1000 diluted mouse anti-his primary antibody (MCA1396, RRID:AB_322084, Bio-Rad) and then for 1 hour at room temperature with a 1:4000 diluted rabbit anti-mouse HRP secondary antibody (SouthernBiotech Cat# 6170-05 ...
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bioRxiv - Molecular Biology 2023Quote: ... His-tagged recombinant protein was purified using Ni-NTA purification with the help of Ni-charged resins (Bio-Rad). Purified protein was further used for kinase assay ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Plasma supernatant was used for the analysis of inflammatory mediators using a multiplex assay (Bio-plex assay; Bio-Rad, Hercules, CA, USA).
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bioRxiv - Neuroscience 2022Quote: Cytokine levels were assessed using Bio-Rad BioPlex assays in both plasma and PFC homogenates (Th1/Th2 rat cytokines Bio-Rad, #171k1002M), according to manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: ... sections were stained with 0.4 µg/mL rabbit -anti-human CD20 polyclonal antibodies (Neomarkers) and 2 µg/mL rat-anti-human CD3 antibodies (clone MCA1477, BioRad). Then ...
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bioRxiv - Microbiology 2020Quote: ELISA plates were coated overnight at 4°C with 0.1 µg/mL of mouse anti-human IgG (human CH2 domain with no cross-reactivity to rhesus macaque IgG; clone R10Z8E9; BioRad) and then blocked for 2 hours ...
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bioRxiv - Immunology 2023Quote: ... IL-17A titres in human serum samples were quantified using a multiplex cytokine panel (Bio-Plex Pro Human Cytokine Assay, BioRad) and a LuminexCorp Luminex 100 machine as per the manufacturer’s instructions.
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Tracing production instability in a clonally-derived CHO cell line using single cell transcriptomicsbioRxiv - Cell Biology 2021Quote: ... The recombinant Human IgG1 Kappa (Biorad, cat.no. HCA192) was used as a positive control ...
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bioRxiv - Microbiology 2019Quote: ... anti–human IgG conjugated with HRP (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2021Quote: ... anti-human IgG-HRP (Bio-Rad 172-1033) and anti-Rat IgG-HRP (Thermo Fisher 31470).
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bioRxiv - Cancer Biology 2023Quote: ... Human GAD1 PrimePCR primers were purchased from BioRad and the sequence for human GAPDH primers are as follows ...
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bioRxiv - Neuroscience 2024Quote: The human cytokine Luminex 8-plex assay (Biorad) was utilised to measure the concentration of IFNγ ...
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bioRxiv - Biochemistry 2022Quote: ... The refolded mature FAM237A was eluted from the C4 reverse-phase column (Hi-Pore reversed-phase column, 4.6 × 250 mm, Bio-Rad) by the acidic acetonitrile gradient.
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bioRxiv - Microbiology 2023Quote: ... The was filtered through a 0.45 μm filter and His-MSI-2 was purified with HisTrapTM FF column (Cytiva) using the Econo Gradient Pump (BIORAD) according to the manufacturer’s protocol and overnight dialysis ...
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bioRxiv - Microbiology 2023Quote: ... Ni-NTA agarose with the bound His-SUMO-SigN was loaded on a Poly-Prep® Chromatography Column (Bio-Rad), washed with P2 buffer and subsequently with the P2 buffer with the 30 mM imidazole ...
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bioRxiv - Neuroscience 2021Quote: ... PAI-1 and insulin) from plasma samples were analyzed by duplicate using the Bio-PlexPro mouse Diabetes group from Bio-Rad (Ref. 171F7001M) by Luminex® 200TM technology as previously described (Ortega Moreno et al. ...
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bioRxiv - Neuroscience 2020Quote: ... Diabetes-associated markers and cytokine levels in the plasma were assessed using the Bio-Plex Pro Mouse Diabetes 8-Plex Assay (Cat# 171F7001M; Bio-Rad, Carlsbad, California) and the Bio-Plex Pro Mouse Cytokine 23-Plex Assay (Cat# M60009RDPD ...
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bioRxiv - Immunology 2022Quote: ... The affinity matrix was resuspended in 150 μl PB for the unbound and full plasma fraction and in 40 μl PB with a 1% blocking buffer background (Bio-Rad, The Netherlands) for the bound fractions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: The level of proinflammatory mediators in plasma (50 μL) was measured with the help of Bio-Plex Pro Mouse Cytokine Standard 23-Plex (Cat# 64209360, Bio-Rad, Hercules, CA) as per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... and metabolic biomarkers of insulin resistance from plasma samples were both analyzed by duplicate using the Bio-PlexPro mouse Diabetes group from Bio-Rad (Ref. 171F7001M), in a Luminex® 200™ technology as previously described (Ortega Moreno et al ...
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bioRxiv - Immunology 2024Quote: ... ACPA IgG levels were quantified based on an in-house standard of pooled RA patient plasma using the Microplate manager software MPM-6 (Bio-Rad Laboratories, Inc.). Unspecific reactivity to the peptide backbone was assessed by applying CArgP2 ...
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bioRxiv - Microbiology 2020Quote: ... Cell culture supernatants were screened for the presence of 27 human cytokines and chemokines using the Bio-Plex Pro Human Cytokine Standard 27-Plex kit (Bio-Rad Laboratory) on a FLEXMAP 3D® analyzer (Luminex ...