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Citations for Bio-Rad :
151 - 190 of 190 citations for Peptide T since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Membrane was washed for 5 min 3x with TBS-T and incubated with secondary goat anti-rabbit-HRP antibody (Bio-Rad) at a 1:10000 ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was synthesized using oligo-d(T) primers and avian reverse transcriptase (iScript cDNA Synthesis Kit, Bio-Rad, Hercules, CA, USA) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed a further 3 times in TBS-T and bound antibodies were visualised using the Clarity Western ECL substrate (BioRad; #1705061) with a ChemiDoc MP system (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... The cDNA was amplified using KAPA HiFi Hotstart ReadyMix PCR kit (Nippon Genetics) with T-100 Thermal Cycler (Biorad, Hercules, CA). The temperature profile of the reaction was 95 °C for 2 min ...
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bioRxiv - Microbiology 2023Quote: ... The blots were washed 3 times in PBS-T and the proteins were visualized using Clarity Western ECL blotting substrate (Bio-Rad) or SuperSignal West Femto (ThermoScientific) ...
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bioRxiv - Microbiology 2023Quote: Membrane was then washed three times with TBS-T and incubated with secondary antibody (Goat Anti-Rabbit IgG StarBright Blue 700 by Bio-Rad) diluted 1:3,000 in blocking solution for 45 min ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Blots were washed four times in TBS-T prior to detection with either ProSignal Dura (Prometheus) or Clarity ECL (Bio-Rad) substrate and exposure to radiography film or imaging on a C-Digit imager (LiCOR).
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bioRxiv - Microbiology 2023Quote: ... Blots were washed three more times in TBS-T before being developed with Clarity Max Western ECL reagent and imaged on a ChemiDoc XRS+ (Bio-Rad).
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bioRxiv - Physiology 2023Quote: ... The membranes were washed in TBS-T and developed using SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo) in a ChemiDoc imager (Bio-Rad). We normalized the protein bands to beta-tubulin with Image Lab software (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibody was washed away with PBS-T and stained proteins were detected by chemiluminescence (Clarity western ECL, Bio-rad). The western blot signal was acquired using Amersham Image Quant8000.
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bioRxiv - Plant Biology 2023Quote: ... the membrane was washed three times with T-TBS and twice with TBS for 5 min each and incubated with Clarity Western ECL substrate (Bio-Rad). After 5 min ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was then washed with TBS-T and chemiluminescence was assessed with the BioRad ChemiDoc MP Imaging System (BioRad, 12003154).
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed three times in PBS + 0.05% Tween-20 (PBS-T) and incubated in goat anti-mouse or goat anti-rabbit horseradish peroxidase (HRP)-conjugated secondary antibody (Bio-rad) diluted 1:5000 in blocking solution ...
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bioRxiv - Plant Biology 2022Quote: ... CLso mature effectors (without signal peptide) were amplified from haplotype A and B infected tomatoes genomic DNA under standard PCR conditions using iProof (Biorad, Supplementary Table 1). Annealing temperatures of primer pairs were calculated using FastPCR (Kalendar et al. ...
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bioRxiv - Neuroscience 2023Quote: ... Two µl of RLN3 peptide were added to the center of a 0.2 μm nitrocellulose membrane (Cat. No.1620146, Bio-Rad, Hercules, CA, USA). Non-specific sites were blocked by incubation with 5% BSA in Tris-buffered saline with 0.1% Tween® 20 detergent (TBS-T ...
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bioRxiv - Molecular Biology 2019Quote: ... The blot was washed 4 times with TBS-T and subsequently incubated with a goat anti-mouse-HRP antibody (Bio-Rad #1706516) for 30 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... Membranes were then washed 3 times in PBS-T followed by a final wash in PBS before signal development in Clarity Western ECL substrate (Bio-rad laboratories) and visualization on a ChemiDoc MP imager.
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bioRxiv - Pathology 2019Quote: ... Blots were then washed five times with TBS-T and signals were visualized by using enhanced chemiluminescence (SuperSignal West Pico, Pierce) and detected using ChemiDoc™ Imaging Systems (Bio-rad).
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bioRxiv - Bioengineering 2021Quote: ... The resulting gene fragment was ligated with the plasmid backbone using a ligase-free ligation method developed in the lab that includes incubation with 10 mM MgCl2 in a thermocycling step in a PCR thermocycler (Biorad, T-100). The cycling programme was as follows ...
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bioRxiv - Cancer Biology 2021Quote: ... membranes were washed 3x with TBS-T and incubated with either Goat Anti-Mouse IgG (H+L)-HRP Conjugate (Bio-Rad, #1721011) or Goat Anti-Rabbit IgG (H+L)-HRP Conjugate (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... except that blocking was done with phosphate-buffered saline plus 0.1% Triton X-100 (PBS-T) containing 5% nonfat dry milk (Biorad Blotting grade Blocker) and 10 mM NaN3 ...
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bioRxiv - Developmental Biology 2021Quote: ... Following 4x 10 min washes with PBS-T the membrane was incubated for 1 min with Clarity Western ECL Substrate (170-5060, Bio-Rad Laboratories) and imaged with Amersham™ Imager 600 (GE ...
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bioRxiv - Physiology 2021Quote: ... the membranes were rinsed three times in TBS-T for 5 min and the proteins were visualized by enhanced chemiluminescense (BioRad, Clarity 1705061) in a BioRad Universal III Hood with Image Lab software v.6.0.1 ...
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bioRxiv - Biochemistry 2023Quote: ... Each membrane was then incubated with the appropriate HRP-conjugated secondary antibodies in TBS-T and visualized by using the Enhanced chemiluminescence substrate (BioRad 170-5060) and ChemiDoc (BioRad).
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bioRxiv - Zoology 2023Quote: ... quantitative R T–PCR was performed with a Bio-Rad CFX96™ Real-time PCR Detection System (Bio-Rad, Hercules, CA, USA) according to standard protocols and cycling conditions ...
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bioRxiv - Cell Biology 2020Quote: ... followed by 1◻h incubation at 4◻°C with HRP-conjugated goat anti-mouse (1:5000 dilution in 1% YE in PBS-T, BioRad #170–6516) secondary antibody ...
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bioRxiv - Genomics 2019Quote: ... Total HIV-1 DNA content of CD4 T-cell DNA isolates was measured by digital droplet PCR (ddPCR, QX200 platform, Bio-Rad, Temse, Belgium), as described by Rutsaert et al.56 The DNA was subjected to a restriction digest with EcoRI (Promega ...
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bioRxiv - Genetics 2021Quote: ... membranes were incubated 2h in TBS-T with 2% BSA containing secondary anti-rabbit antibodies-HRP conjugate (1:2000, #1721019 Bio-Rad, Hercules, CA). After three more washes in TBS-T ...
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bioRxiv - Immunology 2021Quote: ... Purified CD8+ T cells were counted and cell diameter measured using a Moxi Z mini (Orflo, MXZ001) or a TC20 (Bio-Rad, 145-0101) automated counter ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were washed five times in TBS-T before detection with chemiluminescent HRP substrate (Immobilon, P90720) and imaged on a ChemiDoc™ XRS+ system (Bio-Rad, 1708265).
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bioRxiv - Cancer Biology 2021Quote: ... NRF2 (CST, 12721S), KEAP1 (CST, 8047S), PRDX1 (CST, 8499S), GSTO1 (Thermo, PA5-83383)] in 5% BSA in TBS-T (Bio-Rad, 170-6435). Following incubation with primary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... Then membranes were washed 3 times with TBS-T and incubated with goat anti-mouse or anti-rabbit IgG HRP-conjugated secondary antibody (1:10000 in 5% BSA TBS-T, Bio-rad; #1706515, #1706516) for 1 h ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Plates were washed again three times with TBS-T followed by the addition of primary antibodies (neat antivenom and normal horse IgG [BioRad] as negative control) in duplicate ...
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bioRxiv - Plant Biology 2024Quote: ... the membrane was washed twice with the 1x TBS-T buffer and then incubated with the Clarity Western ECL Substrate solution (Bio-Rad, California, USA). Signal detection of the membranes was performed by the Chemi-Doc Imaging system (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... the membranes underwent three times with PBS-T and were then subjected to incubation with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad, CA, USA) for 1 h at room temperature ...
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bioRxiv - Immunology 2024Quote: ... the fractions were eluted with 0.25 M NaCl and biologically active fractions were further analyzed on a hydrophobic interaction column (Macro-Prep® t-Butyl HIC Resin, Bio-Rad 1580090). Three fractions eluted with buffers with pH=7 ...
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bioRxiv - Systems Biology 2023Quote: ... resolved in a 4-12% Invitrogen B-T gel using MOPS buffer and transferred onto a polyvinylidene difluoride (PVDF) membrane using BioRad transblot (Bio-Rad, Hercules, CA, USA). The membrane was blocked with 5% skim milk ...
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bioRxiv - Biochemistry 2023Quote: ... Then the membrane was incubated with mouse monoclonal antibodies against PAR for 60 min in PBS-T/5% milk followed by incubation with the secondary anti-mouse antibodies conjugated with horseradish peroxidase (Bio-Rad, Hercules, CA, USA) for 60 min in the PBS-T/5% milk solution ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Glucagon-like peptide 1 (GLP-1) and Adiponectin were measured using quantitative Bio-Plex Pro™ Mouse Diabetes 8-Plex immunoassay (#171F7001M; Bio-Rad Laboratories, Hercules, CA, USA) and Bio-Plex Pro Mouse Diabetes Adiponectin assay #171F7002M (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... Blocking of the membrane was carried out by incubating in blocking solution for 1 h at room temperature (5% Bio-Rad Blotting-Grade Blocker in TSB-T buffer). Membrane was then incubated with anti-PcdA polyclonal antibody diluted 1:15,000 in blocking solution for 2 h at room temperature.