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Citations for Bio-Rad :
251 - 283 of 283 citations for Penicillin G since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... One µg of DNase treated RNA was used to synthesize cDNA using iScriptTM Select cDNA synthesis kit (catalog#170-8896, Bio-Rad, Hercules, CA) according to the manufacturer’s recommendations ...
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Gatekeeper helix activates Golgi SM protein Sly1 and directly mediates close-range vesicle tetheringbioRxiv - Cell Biology 2020Quote: ... The resulting mixtures were dialyzed (20 kDa cutoff) for ∼18h at 4°C in the dark against 250 volumes of FB160M1 containing 2 g BioBeads SM2 (Bio-Rad, Hercules, CA) per 2 mL of RPL mixture ...
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bioRxiv - Plant Biology 2019Quote: ... The protein concentration was quantified by Bradford assays and 10 μg of total protein for each sample was run on a 12.5% Criterion™ Tris-HCl protein gel (3450014, Bio-Rad, Hercules, CA). Following electrophoresis ...
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bioRxiv - Plant Biology 2020Quote: ... were centrifuged at 5000 x g for 15 min at 4°C and resolved by SDS-PAGE prior to transfer to a PVDF membrane (BioRad, Hercules, CA, USA). The membrane was then incubated with SnTox3 primary antibodies ...
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bioRxiv - Genetics 2021Quote: ... Samples were then centrifuged at 4°C for 5 min at 20,000 g and protein concentrations in the supernatants were measured using the Bradford assay (Bio-Rad Protein Assay Dye). Aliquots equivalent to 1 μg and 3 µg protein were separated on NuAGE 12% Bis-Tris Gels using MOPS 1X (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... Forty µg of denaturated proteins were subjected to a Western blot analysis after 4–15 % MiniPROTEAN® TGX StainFree™ gel electrophoresis (Bio-Rad) followed by probing antibodies against TLR4 or β-Actin (TLR4:1:400 ...
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bioRxiv - Microbiology 2022Quote: ... Insoluble material was then removed by centrifugation at 14,000 x g for 10 min and the supernatant was loaded on a 12% Mini-Protean TGX SDS-PAGE system (Bio-Rad; CA, USA). After staining with Coomassie Blue (ThermoScientific ...
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bioRxiv - Neuroscience 2022Quote: ... A total of 0.2-1 µg of RNA was pre-treated with DNAse and used for cDNA synthesis using the iScript cDNA synthesis kit (Bio-Rad, Irvine, CA). Quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2023Quote: ... 5 µg (PrrF1, PrrF2) or 20 µg (PrrH) of total RNA was electrophoresed on a 10% denaturing urea TBE gel (Bio-Rad, Hercules, CA). The RNA was then transferred to a BrightStar-Plus Positively Charged Nylon Membrane (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... Equal amount of total protein (25 μg/well) was resolved by electrophoresis on 8% Tris-Acrylamide gels and transferred onto 0.2 μm nitrocellulose membranes (Bio-Rad Laboratories, CA, US). Membranes were blocked for 1 h at room temperature in 5% skim milk diluted in Tris-buffered saline supplemented with 0.1% Tween 20 (TBST) ...
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bioRxiv - Physiology 2023Quote: ... Twenty µg (islet sample) or 2 µg (mouse liver sample: positive control) was mixed with 10uL of Laemmli Sample Buffer (Bio-Rad, Hercules, CA) and denatured at 95°C for 10 min ...
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bioRxiv - Physiology 2023Quote: ... 25 µg of total protein was loaded into precast 10 % BIO-RAD Mini-PROTEAN® TGX Stain-FreeTM SDS-polyacrylamide gel (Bio-RAD Laboratories), then electrophoretically transferred to 0.2 µm nitrocellulose membrane (Trans-Blot® TurboTM Transfer Pack ...
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bioRxiv - Genetics 2023Quote: ... Co-IP reactions were rotated overnight at 4°C with 100 µl of SureBeads Protein G Magnetic Beads (BioRad; Catalog# 1614013; Hercules, CA). The bead lysates were washed five times in the co-IP buffer using a DynaMag Magnet (ThermoFisher ...
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bioRxiv - Plant Biology 2023Quote: ... Mixed samples were immediately centrifuged at 200 x g for 5 mins and 20 μl of the supernatant was transferred into 1 ml Bradford reagent (Bio-Rad, CA, USA) in 1.5 ml tubes ...
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bioRxiv - Molecular Biology 2021Quote: ... 30 µg of WCE were separated on mini protein TGX stain-free gel and electroblotted using the PVDF transfer membrane (Bio-Rad, Hercules, CA, USA). The ChemiDoc™ MP imaging system (Bio-Rad ...
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bioRxiv - Cancer Biology 2019Quote: ... 20 μg proteins of the cell line were exposed to a 10% SDS-PAGE gel and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). After blocking the membranes using 5% non-fat milk or bovine serum albumin (BSA ...
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bioRxiv - Plant Biology 2020Quote: ... 0.02 µg of cDNA was used in a 20 µl reaction containing 10 µl of SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad, US), 6 µM of forward and reverse primers and 3 µl of RNAse-free water ...
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bioRxiv - Microbiology 2020Quote: ... Cell suspensions were clarified by centrifugation (20 000 x g, 10 minutes, 4°C) and total soluble protein quantified58 using a kit (Bio-Rad Protein Assay Kit) and BSA as a standard.
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bioRxiv - Cell Biology 2020Quote: ... Lysates were clarified for 5 min at 10,000 × g at +4□C and protein concentration was determined using DC Protein Assay (Bio-Rad Laboratories, Hercules, CA, USA). For immunoprecipitation ...
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bioRxiv - Immunology 2021Quote: ... IL-6, TNF-α, IL-8, IP-10, MCP-1, MIP-1α, MIP-1β, IL-1RA, G-CSF and Eotaxin; Bio-Rad Laboratories Inc.) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Protein extracts (50 μg of protein) in β-mercaptoethanol containing SDS sample buffer were separated in 4% to 12% gradient SDS-polyacrylamide gels (Bio-Rad #456-8094) and transferred to nitrocellulose membranes (Bio-Rad #170-4271 ...
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bioRxiv - Immunology 2021Quote: We mixed 500 μg of extracted cell lysates and 100 μL of patients’ sera with 100 μL of SureBeads™ Protein G Magnetic Beads (Bio-Rad, Hercules, CA) and added phosphate-buffered saline containing 0.2% of Tween 20 (PBS-T ...
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bioRxiv - Cell Biology 2021Quote: ... Protein extracts (50 µg of protein) in β-mercaptoethanol containing SDS sample buffer were separated in 4% to 12% gradient SDS-polyacrylamide gels (Bio-Rad #456-8094) and transferred to nitrocellulose membranes (Bio-Rad #170-4271 ...
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bioRxiv - Cancer Biology 2023Quote: IRX4_PEP1 transfected PC3 cell protein lysate was pre-cleared with 25 μL of SureBeads TM Protein G magnetic beads (BIO-RAD, Catalog number – 1614023) for 1 hour at 4 ºC and the beads were removed three times using a magnetic stand ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µg of each cell protein lysate sample was separated using a hand-cast 5-15% gradient Tris glycine gel (Bio-Rad, Protocol Bulletin 6201) using a custom made 25-well comb.
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bioRxiv - Synthetic Biology 2023Quote: ... 1 μg of dsDNA were added to the competent cells and then transferred to chilled 2 mm gap electroporation cuvettes (Bio-Rad Laboratories GmbH, Germany). Electroporation transformation was done with a single pulse at 1.8 kV ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 to 30 µg of lysate protein was subjected to SDS-PAGE electrophoresis on a 4–15% gradient mini-Protean TGX gel (Bio-rad, Hercules, CA, USA). It was then transferred to a membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitation for the protein lysate was performed by using SureBeads™ Protein G or A Magnetic Beads (purchased from Bio-Rad; catalog numbers1614023 and 1614013). Western blotting experiments were performed by using precast TGX 4-20% gels (purchased from Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA synthesis was performed with 1μg of RNA using PrimeScript reverse transcriptase and reverse transcribed using a PCR Instrumentation C1000 Touch(tm) Thermal Cycler (Bio-Rad Laboratories, Hercules, CA, USA) according to the manufacturers’ protocol ...
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bioRxiv - Microbiology 2019Quote: ... for 5 min in 2 ml screw-cap vials with 2 g of glass beads (2mm) and 200 μl of buffer containing 1 × TE buffer and Chelex®-100 (Bio-Rad Laboratories, CA, USA). Samples were then incubated overnight at 56 °C with 10 μl of Proteinase K (20mg/ml ...
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bioRxiv - Neuroscience 2020Quote: Immunoprecipitation of transfected COS-7 cells or C2C12 myotubes transduced with shVangl2 or shScr was performed as described previously (Strochlic et al., 2012) with SureBeads™ protein G magnetic beads (BioRad, catalog no. 161-4023).
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bioRxiv - Cell Biology 2023Quote: ... was purified on a Q-Sepharose Fast-Flow column attached to an ÄKTA™ FPLC system.17 Purity (>95%) was confirmed by SDS-PAGE and staining with Coomassie Brilliant Blue G-250 (Bio-Rad Laboratories, Hercules, CA).
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bioRxiv - Neuroscience 2021Quote: ... Samples were clarified by a brief centrifugation (10,000 g for 5 min at 4°C) and the supernatants were collected and diluted with 2× reducing Laemmeli buffer (Bio-Rad, Hercules, CA, USA, cat. #1610737). For preparation of cell culture lysates ...