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Citations for Bio-Rad :
451 - 500 of 5537 citations for PCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... were performed using the FIREPol® Master Mix Ready to Load (Solis biodyne, 04-12-00115) and a T100 thermal cycler (Biorad). Sequences of the primers used are the following ones ...
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bioRxiv - Plant Biology 2022Quote: ... the genomic region spanning the gRNA target site was PCR-amplified (primers are listed in Table S1) from WT and gene-edited tree genomic DNA using iProof GC master mix (Bio-Rad) and sequenced ...
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bioRxiv - Pathology 2023Quote: ... One nanogram of cDNA from each sample was mixed with forward and reverse primers (300 nM) and SYBR Green master mix (Bio-Rad). The amplification program was as follows ...
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bioRxiv - Evolutionary Biology 2023Quote: ... qPCR was performed with gene-specific primers using the Taq Pro Universal SYBR qPCR Master Mix (Vazyme #Q712) reaction system on the CFX96 Real-Time detection system (Bio-Rad). Each experiment was performed with three reaction replicates and UBQ10 was used as the internal control for data analysis ...
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bioRxiv - Microbiology 2023Quote: ... and qRT-PCR was performed using AceQ qPCR SYBR Green Master Mix (Vazyme) and corresponding primers on a CFX96 Touch Real-Time System (Bio-Rad). Primers used for amplifications are listed in Table 1 ...
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bioRxiv - Genomics 2023Quote: ... RT-PCR was performed in triplicates and carried out in 20 µl of reaction mixture containing 10 µl of Fast SYBR® Green Master Mix (Biorad), 0.2 µl (10 pmol/µl ...
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bioRxiv - Microbiology 2023Quote: ... qPCR analysis was performed using ChamQ SYBR qPCR Master Mix (Q311, Vazyme) with a CFX ConnectTM Real-Time System (Bio-Rad). Bacillus subtilis (ATCC® 6051™ ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative levels of mRNA were assayed in triplicates using the Universal SYBR Master Mix (MQ101-00, Vazyme) following the manufacturer’s instruction in the CFX Connect System (Bio-Rad Laboratories). Primers for DAT and ACTB are listed in Supplementary Table 1 ...
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bioRxiv - Molecular Biology 2023Quote: qPCR was run with cDNA previously generated from rescue experiments in a 10 µl reaction using iTaq Universal SYBR Green Master Mix (BioRad 1725121) with readout using the Applied Biosystems ViiA 7 real-time PCR system with standard cycling parameters (50°C for 1 min ...
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bioRxiv - Immunology 2023Quote: ... The 5x-diluted RT samples for detection were mixed with ChamQ SYBR qPCR Master Mix (Q311-02, Vazyme, China) according to instruction and then run on CFX 96 (Biorad, USA). The expression level of target genes was normalized by the 2-ΔΔCt method after having checked that the efficiencies of the couple of primers were similar.
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bioRxiv - Cancer Biology 2023Quote: ... QPCR was performed using probe-based primer sets and master mix (Prime Time, Integrated DNA Technologies, Coralville, IA) on a CFX96 real time platform (Bio-Rad). Data was evaluated using Maestro software (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2020Quote: ... by mixing synthetic oil with 20 μL PCR mix containing 2X ddPCR Evagreen supermix (Bio-Rad), 0.9 mM of each primer and 5 μL of template gDNA at 0.5 ng/μL ...
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bioRxiv - Cell Biology 2021Quote: ... and 2.5 µl was used in a reaction mix containing Droplet Digital PCR Supermix (Bio-Rad), TaqMan assay and RNAse free water ...
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bioRxiv - Cancer Biology 2023Quote: ... The synthesized cDNA was subsequently used in a qRT–PCR using IQ SYBR green mix (Biorad) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative PCR was performed on three biological replicates along with three technical replicates using the SYBR-green dye premixed master-mix (Thermo Fischer Scientific) in a C1000 Touch© thermal cycler (BIO-RAD). CT values were obtained using Bio-Rad CFX manager 3.1 ...
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bioRxiv - Plant Biology 2020Quote: ... Reactions were performed with 2 × Taq Master Mix (Vazyme, Nanjing, China) on a Bio-Rad Thermal Cycler (Bio-Rad, Hercules, CA, USA) using the following procedure ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative PCR was performed on three biological replicates along with three technical replicates using the SYBR-green dye premixed master-mix (Thermo Fischer Scientific) in a C1000 Touch© thermal cycler (BIO-RAD). CT values were obtained using Bio-Rad CFX manager 3.1 ...
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bioRxiv - Bioengineering 2022Quote: ... 6 ng of cDNA was used per qPCR reaction consisting of gene-specific primers (Supplementary Information Table 3) and amplified with the PowerSYBR Green Master Mix (Applied Biosystems in a CFX-96 thermocycler (Bio-Rad). Data was acquired using the Bio-Rad CFX Manager 3.1 ...
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ATM-mediated DNA damage response in macrophages primes phagocytosis and immune checkpoint regulationbioRxiv - Immunology 2020Quote: ... and the corresponding 5 μl of Primer Master-mix was added followed by sealing the plate with Microseal ® adhesive sealer (Bio-Rad). The 60S acidic ribosomal protein P0 (rplp0) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Expression levels of ICAM-1 and IL6 mRNAs were quantified using SYBR Green-based primers and the iTaq master mix (Bio-Rad Inc) in the Bio-Rad CFX Real-Time PCR detection system (Bio-Rad Inc) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the cDNA was with Taq Pro Universal SYBR qPCR Master Mix kit (Q712, Vazyme, Nanjing, China) by the CFX96TM Real-Time System (BIO-RAD, USA). The thermal cycles were 95 ℃ for 5 min ...
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bioRxiv - Microbiology 2022Quote: ... burgdorferi 16S rRNA by preparing samples in a 96-well plate using 5 µL of 2X SYBR Green Master Mix (Bio-Rad #1725121), 1 µL of each primer (Forward ...
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bioRxiv - Genetics 2019Quote: ... PCRs were performed with EurobioProbe qPCR Mix Lo-ROX in a CFX96 Real time machine (Bio-Rad) using the following program ...
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bioRxiv - Cancer Biology 2021Quote: ... using RNeasy mini/micro kit and SsoEva Green SYBR mix on CFX96 Real-Time PCR system (BioRad) respectively ...
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bioRxiv - Immunology 2021Quote: ... DNA contamination in the treated RNA samples was assessed by performing a qPCR cycling with tilapia elongation factor 1α (EF-1α) primers using No-RT master mix (absence of reverse transcriptase enzyme provided in iScript™ Reverse Transcription kit, Bio-Rad, USA). The cDNA synthesis (20 µL reactions ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA corresponding to approximately 10 ng of starting RNA was used and qPCR was performed with SYBR green master mix (Bio-Rad Laboratories, Inc.). Primers for real-time PCR were GAPDH-F ...
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bioRxiv - Evolutionary Biology 2022Quote: ... using the Taq Pro Universal SYBR qPCR Master Mix (Vazyme, Nanjing, China) reaction system on the CFX96 Real-Time detection system (Bio-Rad, CA, USA). Each experiment was performed with three technical replicates and the UBQ10 was used as the endogenous control for data analysis.
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bioRxiv - Plant Biology 2023Quote: ... cDNA was diluted 10 fold in nuclease free water and reactions were performed in a Bio-Rad CFX96 system using gene specific primers (Table S1) and Bio-Rad SYBR green master mix (Bio-Rad, Hercules, USA). Each sample was run in triplicate ...
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bioRxiv - Cell Biology 2024Quote: ... 4 µl of the diluted cDNA was added to a Master mix containing 4 µl SsoAdvanced Universal SYBR® Green Supermix (Bio-rad #1725270) and 0.5 µl of a 10 µM stock of each Forward and Reverse primer (Table 5 ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was mixed with Hieff® qPCR SYBR Green Master Mix (Yeasen Biotechnology, 11201ES08) and subjected to qPCR with a sequence detection system (CFX; Bio-Rad Laboratories). The qPCR primers used in this study are described in Supplementary Table S3.
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bioRxiv - Microbiology 2023Quote: ... 1 µL primer 556R (5’ CTTTACGCCCARTRAWTCCG 3’) at 10 µM and 10 µL SYBER® Green Master Mix (Bio-Rad, Veenendaal, The Netherlands). The DNA extraction estimated an average rRNA yield corresponding to 5×10^8 bacterial cells/mL.
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bioRxiv - Genetics 2020Quote: ... Quantitative PCR was performed on a Bio-Rad CFX96 real-time system using SsoFast Evagreen mix (Bio-Rad) and primers are listed in Table S3 ...
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bioRxiv - Genetics 2020Quote: ... real-time PCR was performed in a BioRad CFX Connect Real-Time System using SYBR Green mix (BioRad) according to the MIQE (Minimum Information for Publication of Quantitative Real-Time PCR Experiments ...
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bioRxiv - Molecular Biology 2021Quote: ... 15 µL of PCR mix was added to each well of 96 well ddPCR plate (BioRad Laboratories 12001925) followed by 5 µL of each sample ...
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bioRxiv - Plant Biology 2022Quote: ... qRT-PCR was carried out using the iTaq Universal SYBR Green Super mix (BIO-RAD, Herculese, CA, USA) with D ...
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bioRxiv - Cancer Biology 2023Quote: ... Each ddPCR reaction mix was added to a 96-well PCR plate (PN 12001925, Bio-Rad, Hercules, CA), sealed with a PX1 PCR Plate Sealer (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... DNA samples (3 μl) and master mixes (7 μl) were pipetted into a 96-well PCR plate (Bio-Rad #MLL9651) and PCR amplification performed using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... on cDNA samples using the LightCycler 480 SYBR Green I Master kit in the CFX384 RT-PCR Detection System (Biorad). The identities of the resulting amplicons were checked by melting-curve analysis using the CFX384 software ...
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bioRxiv - Physiology 2023Quote: ... PCR reactions were run in a 384-well format with 10 μL reaction mixture containing SYBR Green I Master (Biorad) (5 μL) ...
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bioRxiv - Molecular Biology 2020Quote: ... qRT-PCRs was performed using the ChamQ SYBR Color qPCR Master Mix (Q411, Vazyme, Nanjing, China) on a Bio-Rad CFX96 (Bio-Rad, Hercules, CA, USA). UBC21 and UBQ10 were used as internal controls for qRT-PCR ...
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bioRxiv - Developmental Biology 2020Quote: Three technical replicates of 10 ul qPCR reactions per sample were prepared using Bio-Rad SsoAdvanced universal SYBR green master mix (Bio-Rad, Hercules, CA 1725271) and were run on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad 1855195) ...
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bioRxiv - Neuroscience 2021Quote: Each sample was run in three technical replicates per gene in a 10 uL qPCR reaction using Bio-Rad SsoAdvanced universal SYBR green master mix (Bio-Rad, Hercules, CA 1725271). Samples were run on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad 1855195) ...
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bioRxiv - Cell Biology 2022Quote: ... Transcript analysis by qRT-PCR were performed using 2× ChamQ Universal SYBR qPCR Master Mix (Viazyme Biotech Co., Ltd., Nanjing, China) and a CFX96-C1000 96-well plate thermocycler (Bio-Rad, Hercules, CA, USA), the 18S rRNA was used as a reference gene25 ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was then amplified by qPCR using specific primers listed in supplementary Table 1 and the SYBR Green Master Mix (ref 1725274, Bio-Rad, Hercules, California, USA). qPCR was performed using the CFX connect real-time PCR system (Bio-Rad ...
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bioRxiv - Genomics 2022Quote: ... All PCRs described above were carried out using KOD One Master Mix (#KMM-101, TOYOBO, Osaka, Japan) with a C1000 Touch thermal cycler (Bio-Rad, Hercules, CA, USA). Primer sequences are listed in Table S3.
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bioRxiv - Microbiology 2024Quote: ... The mixture was subjected to RT-qPCR analysis using the ChamQ Universal SYBR qPCR master mix kit (Vazyme) in a CFX96™ Real-Time System (BioRad, Hercules, CA, USA). RNA from three biological replicates were analyzed and four technical replicates were performed ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was then amplified by qPCR using specific primers listed in supplementary Table 1 and the SYBR Green Master Mix (ref 1725274, Bio-Rad, Hercules, California, USA). qPCR was performed using the CFX connect real-time PCR system (Bio-Rad ...
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bioRxiv - Genetics 2022Quote: ... then 1-5ng added to a PCR mix containing 11 μl 2x ddPCR Supermix for Probe (no dUTP, BioRad), 1.1 μl 20x DMBT1 probe assay (FAM-labelled) ...
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bioRxiv - Genomics 2020Quote: ... The digested DNA was used as a template for qPCR using SYBR-Green PCR 2x mix (1725124, Bio-Rad), InstaQ96 (HiMedia ...
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Molecular profiling of driver events and tumor-infiltrating lymphocytes in metastatic uveal melanomabioRxiv - Cancer Biology 2019Quote: ... qPCR was performed using 2x qPCR SyGreen Mix (PCR Biosystems) and the CFX Connect Real-Time System (Bio-Rad). Data analysis was performed by comparing ΔΔ CT values using Ubiquitin as a reference gene.