Labshake search
Citations for Bio-Rad :
1 - 50 of 9153 citations for Onion Yellow Dwarf Virus OYDV PCR Kits since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2020Quote: ... BiFC was done in onion epidermal cells using PDS-1000 Helios Gene Gun (Bio-Rad) according to the manufacturer guidelines ...
-
bioRxiv - Plant Biology 2022Quote: ... Plasmids were transformed into onion (Allium cepa) epidermal cells using a particle delivery system (BIO-RAD) as previously described (Yang et al. ...
-
bioRxiv - Neuroscience 2024Quote: ... The final virus samples were titered by digital droplet PCR (BioRad) using a REP-specific primer/probe set for libraries and a CMV primer/probe set for recombinant virus (Supplementary Table 2) ...
-
bioRxiv - Plant Biology 2020Quote: ... The corresponding constructs were transiently co-transformed in onion epidermis cells using the particle bombardment (Bio-Rad) method as described previously (Lee et al. ...
-
bioRxiv - Plant Biology 2021Quote: ... Particle bombardment of onion peel cells was performed using biolistics particle delivery system PDS-1000/He (Bio-Rad, USA) as per the protocol of Lee et al ...
-
bioRxiv - Biochemistry 2020Quote: ... Onion epidermal cells were bombarded with 5 μg of each DNA construct using a helium biolistic gun (BIO-RAD). Treated epidermal cells were kept in the dark at room temperature for 16 h before analysis by confocal microscopy (Confocal Olympus FV 1000) ...
-
bioRxiv - Plant Biology 2020Quote: Transient expression in onion cells (bulb sliced to ~16 cm2) was conducted using the Biolistic PDS 1000/He Particle Delivery System (Biorad) (1100 psi ...
-
bioRxiv - Molecular Biology 2020Quote: ... Transient gene expression in onion epidermal cells was performed using a Biolistic PDS-1000/He Particle Delivery System (Bio-Rad) according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2024Quote: ... transient assay in onion cells (bulb sliced to ∼16 cm2) was conducted using the Biolistic PDS 1000/He Particle Delivery System (Biorad) (1100 psi ...
-
bioRxiv - Microbiology 2021Quote: ... with the C-terminal fluorescent tag and an empty vector containing only fluorescent tag was bombarded separately on onion peels containing MS plates using the PDS1000/He system at 1100 psi (BioRad, USA). After 36 hrs ...
-
bioRxiv - Plant Biology 2023Quote: ... The recombinant plasmid was coated with 0.6 μm gold particles (1 μg DNA per 10 mg gold) and then introduced into onion epidermal cells using the particle bombardment method (PDS-1000/He particle delivery system, Bio-Rad). The epidermis was incubated on MS medium in darkness at room temperature (20–25 °C ...
-
bioRxiv - Plant Biology 2022Quote: ... Eight microliters of the micro-particle suspension (30 μl) were used for transformation of epidermal onion cells using the PDE-1000He particle gun (Bio-rad). Before transformation ...
-
bioRxiv - Plant Biology 2024Quote: ... The sub-cellular localization was observed in onion epidermal cells through particle bombardment using Biolistic-PDS-1000/He (Bio-Rad; USA) system (Casas et al. ...
-
bioRxiv - Pathology 2023Quote: Rabies virus N gene was amplified by real-time quantitative PCR using iTaq™ Universal SYBR® Green One-Step Kit (BIO-RAD). The primers are as follow ...
-
bioRxiv - Cancer Biology 2022Quote: ... Virus quantification of KO/activation pool was done by droplet digital PCR (ddPCR) using QX200 Droplet Digital PCR System (Bio-RAD, #1864001).
-
bioRxiv - Plant Biology 2020Quote: Transient expression of pro35S::VAMP714::GFP and ST–RFP constructs was carried out in onion epidermal peels following microprojectile bombardment using the Helios Gene Gun system (Bio-Rad Laboratories, Hemel Hempstead, UK). Plates containing bombarded onion sections were covered with aluminium foil and incubated at 22° C overnight ...
-
bioRxiv - Plant Biology 2022Quote: The GFP and DAI-GFP plasmids were transiently introduced into onion epidermal cells using a helium-driven particle accelerator (PDS/1000; Bio-Rad Laboratories, Hercules, CA, USA). The particle bombardment experiments were performed as described by Arimura et al ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Quantitative PCR (qRT-PCR) was performed using an iQ SYBR Green PCR Kit (Bio-Rad) with forward and reverse primers specific for heme oxygenase 1 (HO1) ...
-
bioRxiv - Microbiology 2021Quote: ... Yellow-pNP supernatant was measured in triplicates at 407 nm (BioRad iMark microplate reader). Readings were normalised using both a Tris-only incubation control and further by bacterial density (optical density reading at 600 nm (OD600)).
-
bioRxiv - Microbiology 2021Quote: Total viral RNA was extracted from 0.1 ml virus stocks using Aurum Total RNA Mini Kit (Bio-Rad) and eluted in 50 μl elution buffer pre-heated to 70°C ...
-
bioRxiv - Systems Biology 2021Quote: Differential expression of ten selected transcripts in response to virus at 3-L1 stage was examined using Droplet Digital PCR (ddPCR) (Bio-Rad, Hercules, CA). Because of the difficulty in obtaining a large amount of gut RNA samples and the limited sensitivity of conventional qPCR technique to detect gene expression at a low level ...
-
bioRxiv - Microbiology 2021Quote: ... A one-step RT-PCR kit (BioRad) was used to detect the viral RNA using Applied Biosystems QuantStudio 12K Flex Real-Time PCR System with the following cycling protocol ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... A SYBR green PCR kit (Bio-Rad) was used for quantitative real-time PCR and was performed and analyzed on a CF96 Touch™ Real-Time PCR Detection System (Bio-Rad) ...
-
bioRxiv - Developmental Biology 2022Quote: ... Q-PCR was performed by using Quantitect SYBR Green PCR Kit (Bio-Rad) with a Real-Time PCR System (Opticon Monitor 3) ...
-
bioRxiv - Microbiology 2020Quote: ... After the PCR products were purified using a PCR Kleen kit (Bio-Rad), a nested qPCR was performed using primers AA55M and L and the SYBR green master mix (Thermo Fisher Scientific).
-
bioRxiv - Molecular Biology 2020Quote: ... Quantitative real-time PCR was performed using iTAQ™ SYBR Green PCR Kit (Biorad) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: iScript Advanced cDNA Synthesis Kit for PCR (BioRad) was used for Reverse Transcription (RT ...
-
bioRxiv - Developmental Biology 2023Quote: ... qRT-PCR was performed using the SYBR Green PCR Master Mix Kit (BIO-RAD, USA). The b2m gene was used as a reference gene (Melgar-Rojas et al. ...
-
bioRxiv - Neuroscience 2024Quote: qRT-PCR was performed using the SYBR Green PCR Master Mix Kit (BIO-RAD, USA) as previously described (Murtha et al. ...
-
bioRxiv - Microbiology 2020Quote: ... All gene transcripts were quantified by quantitative PCR using qScriptTM One-Step qRT-PCR Kit (Quanta Biosciences, #95057-050) on CFX96 real-time PCR system (Bio-Rad). Primer sequences for qPCR were as follow ...
-
bioRxiv - Cell Biology 2022Quote: ... Quantitative PCR was performed using the iScript one step RT-PCR SYBR green kit (Bio-Rad). RT-qPCR was performed in duplicate for each of three independent biological replicates ...
-
bioRxiv - Microbiology 2020Quote: ... quantitative PCR was conducted to analyze the extracted DNA using QuantiFast SYBR Green PCR kit (Biorad) with universal 16S rRNA primers 8F ...
-
bioRxiv - Microbiology 2021Quote: ... cDNA for real time-PCR (RT-PCR) was prepared using the Iscript cDNA synthesis kit (Biorad). For RT-PCR ...
-
bioRxiv - Microbiology 2020Quote: ... All gene transcripts were quantified by quantitative PCR using qScript™ One-Step qRT-PCR Kit (Quanta Biosciences, #95057-050) on CFX96 real-time PCR system (Bio-Rad). Primer sequences for qPCR were as follow ...
-
bioRxiv - Microbiology 2021Quote: ... and anti-canine distemper virus nucleoprotein mouse monoclonal primary antibody (Biorad) in selected fox tissues.
-
Macrophage microRNA-146a is a central regulator of the foreign body response to biomaterial implantsbioRxiv - Cell Biology 2024Quote: ... a qRT-PCR was conducted using the One-Step Quantitative Reverse Transcription PCR kit from Bio-Rad. The qRT-PCR utilized specific primers for the following genes ...
-
bioRxiv - Bioengineering 2020Quote: ... using a SYBR Green PCR premix kit (Bio-Rad, USA). PCR analysis was carried out in triplicate (technical replicates) ...
-
bioRxiv - Genomics 2024Quote: The ZDC Multiplex RT-PCR Assay Kit (Bio-Rad, USA) was used to detect the presence of Zika ...
-
bioRxiv - Immunology 2024Quote: ... using SYBR Green PCR Master Mix Kit (Bio-Rad Laboratories) to examine lung mRNA expression of Cxcl1 ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... The qRT-PCR kits were purchased from BioRad (California, USA), and the ELISA kits were purchased from Peprotech ThermoFisher Scientific (Massachusetts ...
-
bioRxiv - Microbiology 2020Quote: ... IL-22 and TNF-α was analyzed by using quantitative RT-PCR (qRT-PCR) according to the Biorad iScript™ One-Step RT-PCR Kit in a CFX96 apparatus (Bio-Rad, Hercules, CA) with the following primers ...
-
bioRxiv - Cell Biology 2021Quote: ... 20 ng cDNA was used for PCR amplification with the iProof High-Fidelity PCR Kit (Bio-Rad 1725330) for 24 cycles ...
-
bioRxiv - Neuroscience 2024Quote: ... Reverse transcriptase-PCRs (RT-PCR) were carried out using the iScript RT Kit for cDNA synthesis (BioRad, 1708841). Where the final mix include 1000ng of RNA sample and 5µL of iScript RT-PCR mix with reverse transcriptase ...
-
bioRxiv - Biochemistry 2021Quote: ... Virus was inactivated by adding 50 μl of 2X Laemmli buffer (BioRad) and incubating at 95 °C for 10 minutes.
-
bioRxiv - Microbiology 2021Quote: Virus or pseudovirus concentrates were lysed in 4x Laemmli buffer (Bio-rad) with 10% β-mercaptoethanol and run on SDS-PAGE gels ...
-
bioRxiv - Microbiology 2022Quote: Virus or pseudovirus concentrates were lysed in 4x Laemmli buffer (Bio-rad) with 10% β-mercaptoethanol and run on SDS-PAGE gels ...
-
bioRxiv - Cancer Biology 2021Quote: ... qPCR was performed using the SYBR Green PCR Kit (Bio-Rad, SsoAdvanced™ Universal SYBR® Green Supermix ...
-
bioRxiv - Microbiology 2023Quote: PCR reactions were performed with iPoofTM HF kit (Bio-Rad, USA) using 50 ng of genomic DNA as a template in 20 μL of the reaction mix ...
-
bioRxiv - Immunology 2023Quote: ... A SYBR green real-time PCR kit (Cat# 001752A, Bio-Rad) and a CFX96 Real-time Detection System (Bio-Rad ...
-
bioRxiv - Synthetic Biology 2024Quote: ... using iProof™ High-Fidelity PCR Kit (Bio-Rad, Cat. #1725331). The products were purified using 4 µL of ExoSAP-IT™ PCR Product Cleanup Reagent (Applied Biosystems ...