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Citations for Bio-Rad :
651 - 700 of 7342 citations for Nucleic Acid Electrophoresis Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Forty µg of denaturated proteins were subjected to a Western blot analysis after 4–15 % MiniPROTEAN® TGX StainFree™ gel electrophoresis (Bio-Rad) followed by probing antibodies against TLR4 or β-Actin (TLR4:1:400 ...
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bioRxiv - Molecular Biology 2020Quote: ... and the sample incubated at 90°C for 5 min and resolved by electrophoresis through a 4–20% gradient polyacrylamide/SDS gel (Bio-Rad Laboratories, 5671085). Next ...
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bioRxiv - Molecular Biology 2022Quote: ... chromatin samples were dissolved in SDS-containing loading buffer and the electrophoresis was carried out in 8-16% mini-protean TGX Bio-Rad gels (Cat. # 4561105, Bio-Rad, Hercules, CA) gradient acrylamide gels as described (Laemmli ...
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bioRxiv - Immunology 2022Quote: ... Equal amount of total protein (25 μg/well) was resolved by electrophoresis on 8% Tris-Acrylamide gels and transferred onto 0.2 μm nitrocellulose membranes (Bio-Rad Laboratories, CA, US). Membranes were blocked for 1 h at room temperature in 5% skim milk diluted in Tris-buffered saline supplemented with 0.1% Tween 20 (TBST) ...
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bioRxiv - Microbiology 2023Quote: The resulting amplicons and their corresponding lengths were visualized and documented via gel electrophoresis and analysis (Quantity 1 software, Bio-rad, CA, USA).
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bioRxiv - Molecular Biology 2023Quote: ... Lysates cleared by centrifugation (20 µL) were resolved by electrophoresis using 4-20% mini-Protean TGX gels in Tris/Glycine/SDS buffer (Bio-Rad, Hercules, USA). Gels were then transferred onto 0.2 µm nitrocellulose membranes using Trans-Blot Turbo Transfer System gel (Bio-Rad ...
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Polarized Mechanosensitive Signaling Domains Protect Arterial Endothelial Cells Against InflammationbioRxiv - Cell Biology 2023Quote: ... Protein samples were run via sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a polyvinylidene difluoride (PVDF) membrane using BioRad Trans-Blot® Turbo™ Transfer system (BioRad #1704150) and Trans-Blot® Turbo Mini PVDF transfer packs (BioRad #1704156) ...
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bioRxiv - Immunology 2024Quote: ... of cells lysates was subjected to electrophoresis on a sodium dodecyl sulfate (SDS) 7.5% or 4-20% polyacrylamide gel (4561024 or 4561095, respectively, Bio-Rad, California, United States). Proteins were semi-dry transferred to PVDF membranes (1704159SP5 ...
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bioRxiv - Physiology 2019Quote: ... RNA integrity was assessed by capillary electrophoresis (Experion Automated Electrophoresis Station using RNA StdSens Assay, Bio-Rad) with average integrity scores (RQI ...
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bioRxiv - Biophysics 2020Quote: ... We visualized the transcription products by electrophoresis in a denaturant gel (23% polyacrylamide, 7 M urea) run at 800V for 2 h (Bio-rad, Hercules, CA, USA).
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bioRxiv - Molecular Biology 2022Quote: ... samples were solubilized in Laemmli sample buffer and heated at 95°C for 10 min to denature proteins and separated via SDS-PAGE for 40–60 min at 270 V using 4–20% stain-free gradient gels on Criterion electrophoresis cell (Bio-Rad Laboratories, Richmond, CA). Thereafter ...
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bioRxiv - Cancer Biology 2022Quote: Equal amounts of cell lysate were resolved by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). Blocking was performed for 60 min with 5% nonfat dry milk in Tris-buffered saline and Tween 20 ...
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bioRxiv - Immunology 2022Quote: ... Proteins were separated on 10%–12% SDS polyacrylamide gels by electrophoresis and transferred onto nitrocellulose membranes using a Trans-Blot SD Semi-Dry transfer cell (Bio-Rad, Hercules, CA, USA). Membranes were blocked with 5% skim milk for 1 h ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of cell lysate or concentrated conditioned medium were resolved by sodium dodecyl sulfate– polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). The membrane was blocked for 60min with 5% non-fat dry milk in Tris-buffered saline and Tween 20 (1:1000) ...
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bioRxiv - Cancer Biology 2019Quote: ... 20–100 μg of proteins were separated in reducing conditions (2.5% β-mercaptoethanol) by SDS–PAGE (SDS–polyacrylamide gel electrophoresis) and transferred to nitrocellulose membranes (Bio-Rad, Hemel Hempstead, UK) for further processing ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Nuclear and total protein samples were separated on sodium dodecyl sulphate-polyacrylamide gel (SDS-PAGE) electrophoresis and subsequently transferred to polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Hercules, CA, USA). After blocking using 5% of bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene fluoride membrane by using a semi-dry transfer unit (both from Bio-Rad, Hercules, CA, USA). After being blocked in Tris-buffered saline containing 5% non-fat milk and 0.1% Tween-20 ...
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bioRxiv - Cancer Biology 2021Quote: ... 20 to 30 μg of lysate protein was subjected to electrophoresis on a 4–15% gradient mini-Protean TGX gel (Bio-rad, Hercules, CA, USA). It was then transferred to a membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-rad) ...
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bioRxiv - Immunology 2021Quote: ... 20 to 30 µg of lysate protein was subjected to electrophoresis on a 4–15% gradient mini-Protean TGX gel (Bio-Rad, Hercules, CA, USA). It was then transferred to a membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cellular proteins were resolved using sodium dodecyl sulfate-polyacrylamide gel electrophoresis before being electroblotted onto polyvinylidene fluoride membranes (#1704156, Bio-Rad Laboratories, Hercules, CA). The membranes were incubated with the following primary antibodies for 60 min at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples containing 30 μg of total protein were separated by SDS-PAGE for 30 min at 270 V using 4–20% gradient gels on Criterion electrophoresis cell (Bio-Rad Laboratories, Richmond, CA). Proteins were transferred to nitrocellulose membranes ...
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bioRxiv - Bioengineering 2023Quote: ... was subjected to SDS-PAGE using a 4-20% Mini-PROTEAN TGX acrylamide gel on a Mini-PROTEAN Electrophoresis cell (Bio-Rad Laboratories, CA, USA). Electrophoretically separated proteins were transferred to Immobilon-P PVDF membrane (25V/RT/overnight) ...
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bioRxiv - Immunology 2023Quote: ... Western blotting was performed by electrophoresis of 20 µg of proteins on Mini-PROTEAN or Criterion TGX Precast Gels (Bio-Rad, Hercules, CA, USA), followed by electrotransfer to Amersham™ Protran® nitrocellulose membrane (Millipore Sigma) ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 to 30 µg of lysate protein was subjected to SDS-PAGE electrophoresis on a 4–15% gradient mini-Protean TGX gel (Bio-rad, Hercules, CA, USA). It was then transferred to a membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... Equal protein amounts of the different samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto nitrocellulose or polyvinylidene difluoride (PVDF) membranes (Bio-Rad Laboratories, Feldkirchen, Germany) by semidry blotting ...
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bioRxiv - Immunology 2024Quote: ... 20 to 30 µg of lysate protein was subjected to electrophoresis on a 4–20% gradient mini-Protean TGX gel (Bio-rad, Hercules, CA, USA). It was then transferred to a PVDF membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-rad) ...
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bioRxiv - Genomics 2020Quote: ... Electrophoresis was run for 15 minutes at 21V in a Mini-Sub Cell GT electrophoresis tray (Bio-Rad). Subsequently ...
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bioRxiv - Biochemistry 2022Quote: ... in 1% (v/v) acetic acid] for 1 min and photographed using Gel Doc XR system (Bio-Rad).
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bioRxiv - Developmental Biology 2021Quote: ... and automated chip electrophoresis analyses (Experion, BioRad) respectively ...
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bioRxiv - Systems Biology 2022Quote: ... Electrophoresis reagents were purchased from Bio-rad and trypsin from Promega.
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bioRxiv - Neuroscience 2019Quote: ... Mini Protean III Electrophoresis system (Bio-Rad 165-3301
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bioRxiv - Cell Biology 2019Quote: ... using an Electrophoresis Transfer Cell (Bio-Rad). The membrane was blocked in 5% milk for one hour ...
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bioRxiv - Immunology 2021Quote: ... and Experion electrophoresis system (Bio-Rad Laboratories). RNA (400 ng ...
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bioRxiv - Developmental Biology 2023Quote: ... Electrophoresis was performed at 120V (Bio-Rad). Once the proteins resolved ...
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bioRxiv - Bioengineering 2023Quote: ... for electrophoresis in TGS buffer (Bio-Rad) at 100 V for 30 min ...
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bioRxiv - Immunology 2024Quote: ... and Experion electrophoresis system (Bio-Rad Laboratories). cDNA was synthesized from RNA (400 ng ...
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bioRxiv - Bioengineering 2019Quote: Free FAM NHS ester remaining after purification was quantified by polyacrylamide gel electrophoresis (PAGE) run according to the Laemmli protocol51 (adapted in BioRad Mini-PROTEAN Tetra Cell manual). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... and half of each biotinylated fraction were separated into 10% SDS-PAGE gels electrophoresis and transferred to nitrocellulose membranes (Bio-Rad Laboratory, Hercules, CA, USA). Subsequently ...
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bioRxiv - Immunology 2020Quote: ... Protein profile was analyzed using 15% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) as described by Laemmli [11] and stained with Coomassie Brilliant Blue (Bio-Rad, Rockville Centre, NY 11571) [12] ...
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bioRxiv - Immunology 2019Quote: ... total proteins were quantified in paw extracts using the Bicinchoninic acid technique (BCA) and 75-100µg of proteins were loaded on 12% polyacrylamide gels (Biorad). The migration step was followed by a liquid transfer on PVDF membranes (100V 40min ...
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bioRxiv - Biochemistry 2021Quote: ... Approximately 20 ug of acid-extracted protein was run in triplicate on a 4-20% SDS-PAGE gel (BioRad) and a single band corresponding to P1 and P2 was cut out for processing ...
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bioRxiv - Microbiology 2019Quote: ... The gels were then fixed in 12% trichloroacetic acid for 1h and stained with QC Colloidal Coomassie stain (BioRad). Molecular weights were estimated using a calibration curve (Log10 MW vs Rf ...
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bioRxiv - Biochemistry 2020Quote: ... The gels were scanned using Bio-5000 Professional VIS Gel Scanner (Provided by SERVA Electrophoresis GmbH, Heidelberg, Germany) and analyzed with Image Lab Software (Bio-Rad Laboratories Ltd., Hemel Hempstead, UK). Electrophoresis experiments were repeated two times.
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bioRxiv - Biochemistry 2024Quote: ... The purification is assessed by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE; precast 4 to 20 % gradient from Bio-Rad stained with Coomassie Brilliant Blue), but proteins typically elute in the 100 mM fraction ...
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bioRxiv - Cell Biology 2022Quote: ... using a Mini-Protean electrophoresis system (Bio-Rad). Membranes were blocked in 5 % nonfat milk for 2 h ...
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bioRxiv - Bioengineering 2022Quote: ... were loaded into electrophoresis tank (Bio-Rad Laboratories) and filled with 1× Tris/Glycine/SDS buffer ...
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bioRxiv - Genetics 2020Quote: ... using Mini-PROTEAN® Electrophoresis System (Bio-rad) and then transferred to Biodyne B Nylon Membrane (Thermo Scientific ...
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bioRxiv - Immunology 2022Quote: ... for electrophoresis in Tris/Glycine running buffer (BioRad) at 80 V for 15 min followed by 180 V for 1 h ...
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bioRxiv - Molecular Biology 2022Quote: ... resolved by SDS-PAGE electrophoresis (4561086, Bio-Rad) and transferred to nitrocellulose membranes (1704159 ...
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bioRxiv - Microbiology 2022Quote: ... Sodium dodecyl sulphate (SDS) electrophoresis grade from BioRad. Fructose ≥99% from Sigma-Aldrich ...