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Citations for Bio-Rad :
101 - 150 of 8814 citations for Nipah Virus Glycoprotein G Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: The recombinant protein of each vaccine candidate was purified under denaturing conditions using a biologic duoflow™ chromatography system (Bio-Rad Laboratories, Hercules, USA). A HisTrap™ HP column (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2021Quote: ... Virus was inactivated by adding 50 μl of 2X Laemmli buffer (BioRad) and incubating at 95 °C for 10 minutes.
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bioRxiv - Microbiology 2021Quote: Virus or pseudovirus concentrates were lysed in 4x Laemmli buffer (Bio-rad) with 10% β-mercaptoethanol and run on SDS-PAGE gels ...
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bioRxiv - Microbiology 2022Quote: Virus or pseudovirus concentrates were lysed in 4x Laemmli buffer (Bio-rad) with 10% β-mercaptoethanol and run on SDS-PAGE gels ...
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Tracing production instability in a clonally-derived CHO cell line using single cell transcriptomicsbioRxiv - Cell Biology 2021Quote: ... The recombinant Human IgG1 Kappa (Biorad, cat.no. HCA192) was used as a positive control ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were prepared at 25 µg protein/well with 2X laemmli loading buffer and 2-mercaptoethanol and run on pre-cast 4-15% gels (Biorad). Proteins were wet transferred onto PVDF membranes ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 μg of total protein extracts were run on a gradient (15-4%) Polyacrylamide gel (MINIi-PROTEAN TGX Precast Gels, Biorad). Proteins were transferred on nitro-cellulose membrane and checked with Red Ponceau staining for efficient transfer ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... cell were lysed with modified RIPA buffer and EP300 was co-immunoprecipitated using magnetic Sure-bead Protein G (Bio-Rad)-coupled p300 (clone 2033 ...
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bioRxiv - Cell Biology 2022Quote: ... as well as respective inputs (20 µl of extracts corresponding to 50 µg of total proteins) were separated using SDS PAGE electrophoresis and transferred onto nitrocellulose membrane by electro-blotting (SemiDry, BioRad). Western blots were probed with rabbit anti-GFP (1:5000 ...
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bioRxiv - Immunology 2022Quote: ... Total of 15-30µg protein were separated on a 4-12% Bis-Tris SDS PAGE and transferred using BioRad Turbo Blot (1704156, BioRad) onto PVDF membrane ...
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Peripheral neuropathy linked mRNA export factor GANP reshapes gene regulation in human motor neuronsbioRxiv - Neuroscience 2021Quote: ... 5 µg of protein was loaded per well on a 10% TGX 10-well 30µl/well gel (456-1033, Bio-Rad) for 1 hr ...
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bioRxiv - Biochemistry 2020Quote: ... The lysates were cleared for 10 min at 21,000 × g at 4°C and Bio-Rad protein assay reagent (Bio-Rad) was used to determine the protein concentrations of lysates ...
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bioRxiv - Synthetic Biology 2020Quote: ... Soluble and insoluble fractions were separated by centrifugation at 20,000 x g for 30 minutes and protein expression was screened by running both fractions on SDS-PAGE (Bio-Rad) and for selected samples also by negative stain EM ...
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bioRxiv - Cell Biology 2021Quote: Purified substrates and enzymes were centrifuged (16,000 g, 5 min, 4°C) before protein concentration was determined by Bradford assay (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... 10μg/μl of protein was loaded into each lane of 4–15% (mg/100 mL) Tris·HCl polyacryamide gels (Bio-Rad). Protein was transferred to Immobilon-P PVDF membranes (Millipore) ...
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bioRxiv - Immunology 2020Quote: ... first Protein A/G agarose was loaded on Micro Bio-Spin™ Chromatography Columns (Bio-Rad Laboratories, Hercules, CA, USA). After gravity flow ...
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bioRxiv - Genetics 2022Quote: ... the antibodies were prepared by mixing 3 ug of antibody HNRNPK and IgG (as a negative control in a separate tube) with 650 ul of Dilution Buffer and 50 ul of Surebeads Protein G magnetic Beads (BioRad) and left in rotation for 30 minutes at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were cleared by centrifugation at 14,000 g for 15 min at 4°C and protein concentration was determined using the Bradford assay (Bio-Rad). Sodium dodecyl sulfate (SDS ...
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bioRxiv - Immunology 2023Quote: ... 15 to 30 µg of protein sample was processed for separation through SDS PAGE on 4–20% Criterion TGX precast gradient gels (BioRad). Gel run consisted of 15 min run at 70V followed by 45 min run at 100V ...
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bioRxiv - Biochemistry 2023Quote: ... The lysates were pelleted at 10,000 g for 10 min and the supernatants were used to determine the protein concentration using Bradford assay (Bio-Rad). After a preclearing step with Protein G Sepharose (Sigma) ...
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bioRxiv - Cancer Biology 2023Quote: ... 30-50 µg of nuclear proteins were loaded onto a 10% SDS-PAGE and transferred on nitrocellulose membrane using TransBlot apparatus (BioRad). Membranes were subsequently incubated with TBS Tween-20® 0.01% ...
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bioRxiv - Physiology 2023Quote: ... and left ventricle of wild-type C57 Bl6 mice was incubated with PC2 antibody (10 ug, Alomone laboratories) overnight complexed to magnetic protein G beads (Biorad). Following 3 rounds of washing ...
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bioRxiv - Microbiology 2023Quote: ... followed by immunoprecipitation with 5 µg appropriate antibodies or corresponding IgG control using magnetic protein A/G beads (BIO-RAD). After several rounds of washing with low-salt wash buffer (20 mM Tris-HCl ...
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bioRxiv - Microbiology 2021Quote: Concentrated PV or virus was mixed with 4x Laemmli sample buffer (Bio-Rad) with 10% β-mercaptoethanol and run on SDS-PAGE gels ...
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bioRxiv - Molecular Biology 2021Quote: ... and the equivalent of 50 μg protein input (elution) were analyzed on a 4%-12% gradient XT precast Criterion gel using XT-MOPS buffer (Bio-Rad).
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bioRxiv - Molecular Biology 2019Quote: ... 5-15 µg of protein was resolved by SDS/PAGE and transferred to nitrocellulose membranes using the Trans-Blot Turbo System (Bio-Rad). Membranes were blocked in 5% milk in phosphate buffered saline with 0.1% Tween (PBS-T ...
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bioRxiv - Cancer Biology 2020Quote: ... 30 μg of protein was loaded into each lane of a TGX-stain-free pre-cast 4–20% SDS gel (Biorad, 4568094), total protein was quantified upon stain-free gel imaging ...
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bioRxiv - Cell Biology 2020Quote: ... Triton-insoluble material was then pelleted at 15890 x g for 10 min and protein concentration measured with the DC assay (Bio-rad). For immunoblot analysis ...
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bioRxiv - Plant Biology 2022Quote: ... The extract was centrifuged at 18,000 g for 10 minutes at 4 °C and the protein content of the supernatant determined using the Bradford method (Bio-Rad) in a spectrophotometer measuring the absorbance at 595 nm and then eluted in Laemmli 2X buffer with 8 % β-mercaptoethanol (95 °C for 5 minutes) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 150mM DTT was then added to each sample followed by the pelleting of membrane debris and undigested genomic DNA at 21000 x g for 5 min before the supernatant were separated on a Mini-PROTEAN TGX protein gel (Biorad, USA). Total protein was detected in gel by SYPRO Ruby Protein Gel Stain (Thermo Scientific ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 7 μg of nuclear protein extract was separated by SDS-PAGE and transferred to PVDF membrane (Trans-Blot Turbo System, Bio-Rad). We followed standard immunoblot procedures using primary antibodies raised against the Xpress tag (ThermoFisher R910-25 ...
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bioRxiv - Synthetic Biology 2020Quote: ... 10 to 25 μg for experiments that were imaged digitally) was loaded onto a 4–15% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and run either at 50 V for 10 min followed by 100 V for at least 1 h ...
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bioRxiv - Immunology 2022Quote: ... was centrifuged at 500 x g for 10 min Protein levels in the supernatant were measured by Bradford assay (Bio-Rad), and cytokine levels were measured using a custom multiplex ELISA (ThermoFisher Scientific).
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bioRxiv - Developmental Biology 2021Quote: ... Barcoded samples were then pooled and aliquoted into individual ChIP reactions with Protein A/G magnetic beads (BioRad; 161- 4013/23) coupled with the desired antibodies (5 ug each of H3K27me3 {Millipore 07-449} ...
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bioRxiv - Neuroscience 2022Quote: ... 200 µg of protein extracts were loaded and separated on a 10% SDS-PAGE gels and transferred onto PVDF membranes (Bio-Rad). Membranes were blocked with a 3% BSA in TBS-T (Tris-Buffered Saline with Tween-20 containing in mM ...
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bioRxiv - Developmental Biology 2022Quote: ... Western blot analysis was performed by loading 7.5 g of total protein per lane into 7.5% Tris-glycine SDS-PAGE gels (Bio-Rad, catalog #4561026) and transferring to methanol-activated PVDF membranes (Bio-rad ...
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bioRxiv - Immunology 2022Quote: 2 µg of GST or GST-IFITM3 protein was loaded into a 12% acrylamide Criterion XT Bis-Tris Precast Gel (Bio-Rad). Electrophoresis was performed with NuPage MES SDS Running Buffer (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: 40 µg of proteins were separated using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) on a 10% acrylamide gel (BIO-RAD) and transferred to nitrocellulose membrane by semi-dry blotting using the Trans-Blot Semi-Dry Transfer Cell (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... The lysates were centrifuged at 18,000 x g for 30 minutes at 4 °C and the supernatant was incubated with SurebeadsTM Protein A Magnetic Bead (Bio-rad) conjugated with rabbit monoclonal DYKDDDDK antibody at RT for two hours ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 20 μg protein was denatured at 95 °C for 5 min and loaded on a 4-20% precast polyacrylamide gel (BioRad, 4568094) for gel electrophoresis ...
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bioRxiv - Cell Biology 2023Quote: ... 200 μg aliquots of mitochondria were flash-frozen in liquid nitrogen after measuring the protein concentration by a Bradford assay (Bio-Rad) and stored at −80°C.
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bioRxiv - Cell Biology 2024Quote: ... Samples were centrifuged for 30 min at 20,000 x g at 4 °C and protein concentrations of the supernatants were measured with standard Bradford assay (Bio-Rad Laboratories). 25 or 50 µg of proteins were diluted in Laemmli buffer 3× (188 mM Tris-HCl ...
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bioRxiv - Microbiology 2021Quote: ... serial dilutions of recombinant NS1 Antigen (Ag) (Bio-Rad) was used ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μg of total cell proteins were run in a 12% SDS-polyacrylamide gel and blotted to the PVDF membrane (Bio-Rad, USA). Membranes were incubated overnight at 4 °C with mature BDNF primary antibody (ThermoFisher OSB00017W ...
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bioRxiv - Neuroscience 2019Quote: ... and 50 μg protein per sample were run on an SDS-PAGE gel and transferred to an Immun-Blot PVDF Membrane (Bio-Rad Laboratories) using standard protocols ...
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bioRxiv - Cell Biology 2021Quote: ... 10 µg of the cleared lysate were separated by SDS-PAGE using 4%-15% Mini-PROTEAN TGX Precast Protein gels (Bio-Rad, #4561085) and blotted onto a Amersham Protran Premium Western blotting nitrocellulose membrane (Sigma ...
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bioRxiv - Physiology 2021Quote: ... 10 μg of total protein for each sample was separated by SDS-PAGE and transferred to Immun-Blot PVDF membrane (Bio-Rad Laboratories). Subsequently ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared by centrifugation at 4°C for 15 minutes at 16,000 g and protein concentration was measured by the Bradford method (Bio-Rad, Hercules, USA). Next ...
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bioRxiv - Cancer Biology 2019Quote: ... Lysates were precleared with control antibody plus 30 µL of 1:1 Protein-A/G magnetic beads (BIO-RAD, Hercules, CA, USA) for 1 h at 40C ...
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bioRxiv - Cancer Biology 2020Quote: 10 μg of protein samples harvested from xenografted human cells were separated in pre-casted 4-15% gradient SDS gels (Biorad, #456-1084) and transferred to nitrocellulose membranes (Millipore #IPVH00010) ...