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Citations for Bio-Rad :
301 - 350 of 4202 citations for NGS ChIP Seq kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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β-catenin programs a tissue-specific epigenetic vulnerability in aggressive adrenocortical carcinomabioRxiv - Cancer Biology 2022Quote: ... reactions to verify ChIP efficacy were carried out in triplicate on specific positive control genomic regions using SYBR Green Supermix (Bio-Rad).
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bioRxiv - Developmental Biology 2023Quote: ... was generated by co-encapsulating the suspension with barcoded beads in in the 10X microfluidic chip and RT-PCR was performed in a C1000 Touch Thermal Cycler (Bio-Rad) with one of two programs ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was prepared as described in RNA preparation and integrity was assessed on an Experion StdSens RNA Chip (Bio-Rad; 7007103). RNA-Seq libraries were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... qPCR was performed in triplicate using the DNA of at least three independent ChIP experiments in a CFX96 Detection System (Bio-Rad). Quantitative analysis was carried out with the CFX96 Manager Software (version 3.1 ...
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bioRxiv - Immunology 2019Quote: ... and 100 ng of RNA was reverse transcribed using iScript (Bio-Rad). Exon-spanning pre-developed assays for each of our targets were purchased from Thermo Fisher/Applied Biosystems ...
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bioRxiv - Genomics 2024Quote: ... 4.5 µl of sample were used to generate single cell RNA-Seq libraries by following the Illumina Bio-Rad SureCell WTA 3’ Library Prep Guide (Illumina, San Diego CA and Bio-Rad, Hercules, CA) and using the ddSEQ Isolator for Single Cell Sequencing (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... Ten ng of RNA was used as input for ddRT-PCR (Bio-Rad) according to the manufacturer’s protocol with the below assays ...
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bioRxiv - Genomics 2022Quote: ... DNA template (2.5 ng) was mixed with 1× EvaGreen ddPCR Supermix (Bio-Rad), 200 nM gene-specific primers (Supplemental Table S17 ...
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bioRxiv - Pathology 2023Quote: ... 8 ng of cDNA was used for the SYBR green reagent (BioRad 1725270) reaction for qPCR analysis (BioRad) ...
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bioRxiv - Cell Biology 2023Quote: 1,000 ng of RNA was reverse transcribed using iScript (BioRad, Hercules, CA, USA) and qPCR was performed using iTaq Universal SYBR Green Supermix (BioRad ...
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bioRxiv - Physiology 2024Quote: ... 500 ng RNA was used for reverse transcription using iScript reagent (Bio-Rad), and the products were analysed by qRT-PCR (LightCycler480 ...
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bioRxiv - Genomics 2019Quote: qRT-PCR was conducted to validate the differentially expressed miRNA found in the microRNA-Seq analysis on the iCycler iQ PCR system (Bio-Rad, Hercules, CA, USA). The RT-PCR reactions were performed with a QuantiTect SYBR Green PCR Kit (Qiagen ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell concentration was calculated by adding 10 µL of cell suspension to chip for Biorad TC20 Automated Cell Counter (Bio-Rad Laboratories, Richmond, CA).
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bioRxiv - Developmental Biology 2020Quote: ... cDNA was generated using 50 ng RNA and iScript Reverse Transcription Supermix (Bio-Rad), then diluted 1:10 in UltraPure DNase/RNase-Free Distilled Water (ThermoFisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... 200 ng of the total RNA was reverse transcribed to cDNA (BIO-RAD, #1708841). The qPCR was run on Viia 7 RT-PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2019Quote: ... and 5 ng of DNA template using an automated thermal cycler (BioRad, Hercules, CA). The ITS1 locus was amplified following initial denaturation at 94°C for 1 min ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Total mRNA (600 ng) was reverse transcribed using iScript Reverse Transcriptase Supermix (Bio-Rad). Quantitative real-time PCR was performed using iQ Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Physiology 2021Quote: ... 20 ng of total DNA and the SYBR® Green Master Mix (Bio-Rad). b-Actin was amplified as an internal ...
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bioRxiv - Immunology 2020Quote: ... 0.1 ng of template DNA was combined with 10 µL iQ supermix (Bio-Rad) and 2 µL each of 20 µM F primer ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Total mRNA (600 ng) was reverse transcribed using iScript Reverse Transcriptase Supermix (Bio-Rad). Quantitative real-time PCR was performed using iQ Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Biophysics 2023Quote: ... 50 ng of cDNA were used for each reaction with iTAQ Universal Sybergreen (Biorad), using the QuantiStudio 6 Flex system (Applied Biosystems) ...
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bioRxiv - Biochemistry 2021Quote: ... D3_Sof protein was immobilized covalently on the surface of one of the vertical channels of the GLC sensor chip (Bio-Rad Laboratories, Hercules, CA USA) to a 100 RU level ...
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bioRxiv - Plant Biology 2020Quote: ... was performed using 30 ng cDNA and the iQ SYBR Green Supermix (Bio-Rad Laboratories). UBIQUITIN10 (At4g05320 ...
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bioRxiv - Cell Biology 2021Quote: ... and 5 ng/μl of DNA was subjected to Real-Time PCR analysis (Bio-Rad) using Mouse Mitochondrial DNA Copy Number Assay kit (Detroit R&D ...
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A gut-secreted peptide controls arousability through modulation of dopaminergic neurons in the brainbioRxiv - Neuroscience 2020Quote: ... 500 ng of RNA were used for the retrotranscriptase reaction (Biorad iScript cDNA Synthesis #1708890). DNA product was diluted 1:10 and used to set up quantitative PCR reactions using Sybr Green (Biorad #170-8880) ...
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bioRxiv - Neuroscience 2022Quote: ... 800 ng of extracted RNA was used for reverse transcription with iScript Reverse Transcriptase (BioRAD). The fold change of various genes was calculated through the delta-delta CT method using glyceraldehyde 3-phosphate dehydrogenase (GADPH ...
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Bacterial Polyphosphates Induce CXCL4 and Synergize with Complement Anaphylatoxin C5a in Lung InjurybioRxiv - Immunology 2022Quote: ... using 1-2 ng cDNA per sample complemented with iQ SYBR®Green Mastermix (BioRad) and specific forward and reverse primers at a concentration of 0.5 µM each ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA (50–100 ng) was used for cDNA synthesis with iScript reagent (Bio-Rad). qPCR was performed using a CFX384 detection system (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... Purified RNA (500 ng) was reverse-transcribed using the iScript cDNA synthesis system (Bio-Rad). The resulting cDNA was used for real-time quantitative PCR using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... and then 200 ng of RNA was reverse transcribed using the iScript RT Supermix (Bio-Rad), following instructions from the manufacturers ...
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bioRxiv - Neuroscience 2022Quote: ... 150 ng of RNA sample was incubated with iScript™ reverse transcriptase (cat# 1708891, Bio-rad) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... RT-qPCR reactions were performed in triplicates using 20 ng of cDNA with SYBR Green (BioRad) and were run on a CFX96 Touch™ PCR Machine for 39 cycles at ...
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bioRxiv - Biochemistry 2020Quote: ... cDNA was synthesized from 100-200 ng of total RNA using iScript Supermix (Bio-Rad, 1708840) containing random hexamer primers ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesized from 100-200 ng of total RNA using iScript Supermix (Bio-Rad, 1708840) containing random hexamer primers ...
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bioRxiv - Synthetic Biology 2019Quote: ... electroporating 1—10 ng plasmid with the EC1 setting of a Micropulser (Bio-Rad Laboratories, Hercules) and recovering for 1 hour at 37 °C in SOC media before plating on selective LB-agar.
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bioRxiv - Physiology 2021Quote: ... 20 ng of template cDNA per reaction and iQ™ SYBR® Green Supermix (Bio-Rad) were used for real-time PCR with gene-specific primers (Supplemental Table 1 ...
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bioRxiv - Cancer Biology 2021Quote: ... 600 ng of purified RNA was used for reverse transcription with the iScript supermix (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... 50 to 100 ng of total cDNA was used in real time PCR using SybrGreen (BIORAD). Sequence of primers used in real time PCR are detailed in Supplementary Table 2 ...
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bioRxiv - Neuroscience 2023Quote: ... RT-qPCR reactions were performed in triplicates using 20 ng of cDNA with SYBR Green (BioRad) and were run on a CFX96 Touch™ PCR Machine for 39 cycles at ...
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bioRxiv - Physiology 2024Quote: ... 20 ng of template cDNA per reaction and iQ™ SYBR® Green Supermix (Bio-Rad) were used for real-time PCR with gene-specific primers (Supplementary Table 4 ...
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bioRxiv - Microbiology 2020Quote: ... and 100 ng of total RNA was subjected to RT-PCR using a PCR thermocycler (Bio-Rad). The RT-PCR protocol consisted of 50°C or 30 min followed by initial denaturation at 94°C for 2 min ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was synthesized from 500 ng of extracted RNA using the iScript reverse transcription Supermix (Bio-Rad). Real-time quantitative PCR was performed in 4 technical replicates with the SsoAdvanced Universal Probes Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA was generated using 250 ng of RNA with the iSCRIPT reverse transcriptase (BioRad, Hercules, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... Approximately 150 ng of DNA sample was loaded together with CHEF DNA Size Standard Ladder (BIO-RAD) and Quick-Load 1 kb Extend DNA Ladder (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... 500 ng of purified RNA was used to synthesize cDNA using iScript Reverse Transcriptase Supermix (BioRad, 1708841). cDNA was combined with gene specific primers and iTaq Universal SYBR green master mix (BioRad ...
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bioRxiv - Plant Biology 2021Quote: Purified His and GST fusion proteins or GST alone (500 ng) were blotted onto nitrocellulose membrane (BioRad). The nitrocellulose membrane was rinsed with TBS-T buffer (10 mM Tris-HCl at pH 7.4 ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized from 500 ng total RNA using Iscript reverse transcriptase (Bio-Rad, Hercules, CA, USA), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... or 500 ng to 3 µg for integrative plasmids (MicroPulserTM electroporator Biorad in 2 mm cuvettes (Eurogentec) at 25 µF ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 ng RNA was reverse-transcribed into cDNA using the iScript Reverse Transcription Supermix (Bio-Rad, #1708841). Minigene isoforms were amplified by Taq PCR from 2 uL cDNA using forward and reverse primers complementary to the vector sequence (RG6_F ...
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bioRxiv - Cell Biology 2020Quote: ... then 50 ng gDNA was used per qPCR reaction using iQ SYBR Green Supermix (Bio-Rad 1708880). Mycoplasma-specific primer sequences are listed on Table 3 ...