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Citations for Bio-Rad :
251 - 300 of 692 citations for LAG 3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... with or without CLEC-2 antibody (3 μg/ml final concentration; Bio-Rad, Oxford, UK). The plate was incubated in the dark for the indicated time and the reaction was stopped by addition of 200 μl 1% ice-cold formalin ...
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bioRxiv - Synthetic Biology 2021Quote: Supernatant or periplasmic fractions were diluted 3:1 in 4× Laemmli sample buffer (Bio-Rad) and were boiled at 100 °C for 10 min ...
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bioRxiv - Immunology 2020Quote: ... Development was performed after 3 washes in TBS-T and using Clarity ECL reagents (BioRad) and automatic exposure set up on ChemiDoc imaging system (BioRad).
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bioRxiv - Microbiology 2020Quote: ... The membrane was then washed 3 times in TBST and once in TBS (Bio-rad) before being exposed to 2ml of Western Lightning Plus-ECL ...
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bioRxiv - Plant Biology 2022Quote: ... The membrane was washed 3 times before image acquisition by ChemiDoc Touch Imaging System (Biorad). sRNA libraries were constructed with QIAgen miRNA library (QIAgen #331502 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Membranes washed 3 × 5 minutes in TBST then incubated in ECL reagent (BioRad, 170-5061) and imaged using a Chemidoc MP (BioRad) ...
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bioRxiv - Biochemistry 2020Quote: ... The 3 mg/mL protein sample was injected onto NGC Liquid Chromotography System (Bio-Rad) equipped with a HiPrep Sephacryl S-500 HR column equilibrated in FPLC buffer and separated into 156 ...
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bioRxiv - Molecular Biology 2019Quote: ... in Tris-glycine-SDS running buffer or 3-8% Criterion XT Tris-acetate gels (BioRad) in Tris-acetate-SDS running buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein samples were diluted (3:1) with a 4x Laemmli sample buffer (Biorad, Cat# 1610747) and heated at 98 °C for 5 min ...
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bioRxiv - Molecular Biology 2019Quote: ... after 3-4 hrs the beads were passed through Econo-Pac chromatography columns (Bio-Rad) and washed with 20x bead volume of wash buffer in the following order ...
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bioRxiv - Cell Biology 2020Quote: ... Gene expression was measured with the BioRad CFX Man 3 (Bio-Rad laboratories, CA, USA). The measured gene expression was corrected with housekeeping gene Gapdh ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were then blocked with 5% skim milk or 3% blotting-grade blocker (BioRad 1706404) for 1 hr ...
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bioRxiv - Immunology 2021Quote: ... blocking was conducted with 100ul per well of 3% milk solution made from Bio-Rad nonfat dry milk powder and 1xPBS ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were separated by 3-8% or 8-16% SDS-PAGE (NU-PAGE or BioRad) and transferred to immobilon-P membranes (Millipore) ...
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bioRxiv - Biochemistry 2021Quote: ... Gels were dried (3 hr, 80 °C) on a Model 583 Gel Dryer (Bio-Rad) and exposed to a phosphor screen ...
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bioRxiv - Microbiology 2022Quote: ... listed in Table 3 on the CFX96 Touch Real-Time PCR Detection System (Bio-Rad). Gene expression was calculated using the ΔΔct method using hprt as a housekeeping gene and normalized to the average WT S ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cell-free reactions were boiled for 3 min in 2x Tricine buffer (Bio-Rad, #1610739), loaded in 16.5% Mini-PROTEAN polyacrylamide Tris/Tricine gels (Bio-Rad ...
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bioRxiv - Genomics 2023Quote: ... 3-2) Protein Enrichment: The serum samples were processed with a ProteoMiner kit (Bio-Rad) and the protein concentration was determined using the BCA and fluorescence assays ...
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bioRxiv - Microbiology 2023Quote: ... probe 5’-TGCAGTCCTCGCTCACTGGGCACG-3’ using the following conditions on a CFX96 Real Time PCR (Biorad): 50°C for 5 minutes ...
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bioRxiv - Immunology 2023Quote: ... The membrane was blocked with 3% skin-milk-powder in Tris-buffered saline (Bio-Rad) with 0.1% Tween® 20 ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 3 µg protein was separated by SDS-PAGE gel electrophoresis (Bio-Rad) and transferred to 0.2 µm nitrocellulose membrane (Amersham™ Protran™ ...
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bioRxiv - Microbiology 2023Quote: ... 40 µg of proteins were loaded onto 3–8% precast polyacrylamide gel (Bio-Rad, 3450129). After migration ...
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bioRxiv - Bioengineering 2023Quote: ... for 1–3 hours at 100 V and then transferred to PVDF membrane (Bio-Rad) at 40 V for 2–8 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... bromophenol blue) was applied onto IPG strip (7 cm, pH 3-10, Bio-Rad, 1632000) for 24 h ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA bands were stained with 3 × GelRed and visualized on a Chemidoc imager (Bio-Rad).
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bioRxiv - Biochemistry 2024Quote: ... DNA bands were stained with 3 × GelRed and visualized on the Chemidoc imager (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... and qPCR was then performed on a Quantstudio 3 with iTaq Universal SYBR Green (Biorad). RQ values were calculated using a standard curve ...
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bioRxiv - Microbiology 2020Quote: ... Analyses were performed with the Extended Bio-Plex Pro Human Cytokine 48-Plex Screening Panel (Biorad, Munich, Germany) as described previously 72 and using a 2-laser reader allowing simultaneous quantification of cytokines and chemokines ...
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bioRxiv - Cancer Biology 2019Quote: ... Collected sera were subjected to ELISA by capturing with human anti-rituximab idiotype antibody (HCA186, Bio-Rad Laboratories), detected with rat-anti-rituximab-HRP antibody (MCA2260P ...
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bioRxiv - Bioengineering 2020Quote: ... Isolated EVs were stained with PE-labelled anti-human TNFR1 (Bio-Rad, cat #MCA1340PE, clone H398, lot 0407) and APC-labelled anti-mouse gp130 antibodies (R&D Systems ...
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bioRxiv - Immunology 2022Quote: ... We used mouse anti-human HLA-ABC (W6/32) conjugated to Alexa Fluor® 647 (MCA81A647, Bio-Rad), which is known to also react with MHC-I of African Green monkey from which Vero E6 cells are derived(48 ...
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bioRxiv - Immunology 2019Quote: ... fatty acid free BSA Efflux was measured after a 4-hour incubation period with 0µg/ml and 50µg/ml of human HDL (#5685-2004, BioRad). Supernatants were collected and the cells lysed with 1% (w/v ...
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bioRxiv - Immunology 2020Quote: ... The binding was detected by an HRP-conjugated anti-human IgG (Fc) CH2 Domain antibody (Bio-Rad MCA647P) used at a 1:5,000 dilution at RT for 1 h and developed as described above.
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bioRxiv - Neuroscience 2023Quote: ... and Bio-Plex Pro Human Inflammation Panel 1 (37-plex) (Cat. No. 171AL001M) from Bio-Rad (Hercules, CA).
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bioRxiv - Developmental Biology 2022Quote: ... Membranes were washed 3 more times in TBST and imaged on a ChemiDoc Imager (Bio-Rad) using Clarity western ECL substrate (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... Plates were imaged after 3 days at 30°C using the Chemidoc Touch imaging system (BioRad) on colorimetric setting.
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bioRxiv - Genetics 2021Quote: ... were determined using Bio-Plex Pro TGF-β 3-Plex Assay (171W4001M) (Bio-Rad, Hercules, CA).
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bioRxiv - Neuroscience 2020Quote: ... the soluble fraction from 3 months old APPPS1 mice have been quantified using Bradford assay (Biorad) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Blots were washed 3 times with TBST and then incubated with Clarity ECL substrate (Bio-Rad). Blots were imaged using a ChemiDoc MP gel doc system (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... Samples were boiled for 3 minutes and separated on a 4-20% precast polyacrylamide gel (Biorad).
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bioRxiv - Neuroscience 2020Quote: ... Membranes were then wash 3 × 10 min with TBST and detected using chemiluminescence substrate (Bio-Rad).
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bioRxiv - Cell Biology 2021Quote: ... 2-3 μg of total protein were loaded onto 10% SDS-polyacrylamide (29:1 Bio-Rad) gels with 10 μM phostag reagent (FUJIFILM Wako Chemicals ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were resolved on 7 cm pH 3-10 immobilized pH-gradient (IPG) strips (Bio-Rad), under mineral oil ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were washed with TBST 3 times and imaged using the ChemiDoc MP imaging system (BioRad).
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bioRxiv - Molecular Biology 2020Quote: ... Normalised samples (1 – 3 µg) were electrophoresed on 12% Mini-Protean TGX Stain-Free gels (BioRad) or 4-20% Criterion TGX Stain-Free Precast gels (BioRad ...
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bioRxiv - Cell Biology 2021Quote: ... The beads were washed 3 times and then eluted using 2 × Laemmli Sample Buffer (Bio-Rad). The elution was subjected to SDS PAGE to run the sample into the lane ...
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bioRxiv - Systems Biology 2022Quote: ... Each aliquot was heated to different temperatures for 3 minutes in a PCR machine (Bio-Rad), with the temperature range being 42-64 °C and 2-degree intervals ...
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bioRxiv - Biochemistry 2020Quote: ... rinsed 3 times and read by the Bio-Plex (Bio-Rad, Bio-Plex MAGPIX Multiplex Reader). Each experiment was done in duplicate and repeated with media from 3 independent experiments (n=3).
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bioRxiv - Systems Biology 2020Quote: ... The samples were digested for 3 hours in a thermal cycler at 37 °C (BioRad T1000). Samples were cooled to room temperature before TMT labeling (TMT11 plex kit & TMT-Pro 16plex ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were eluted using a 20:7:3 mixture of buffer: 4x Laemmli sample buffer (Biorad):10x NuPage sample reducing agent (Invitrogen ...