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Citations for Bio-Rad :
501 - 550 of 635 citations for L lactate dehydrogenase B chain LDH B Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: Samples in 1x Laemmli loading buffer (generally equivalent to 1 µL replication reaction or chromatin recovered from 8 µL plasmid pull-down) were resolved on 10% or 4-15% acrylamide Mini-PROTEAN or Criterion TGX precast gels (Bio-Rad) and transferred to PVDF membranes (Perkin Elmer) ...
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bioRxiv - Immunology 2021Quote: ... 1/10 dilutions were used in triplicates with 0.2 μM primer and 10 μL LightCycler 480 SYBR Green I Master in a 20 μL reaction and qPCR executed in a 98- well block on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad). Absolute transcript levels for TDP-43 ...
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bioRxiv - Cancer Biology 2019Quote: ... The PCR reactions were performed with 0.2 µl of cDNA template in 25 µl of reaction mixture containing 12.5µl of iQ SYBR Green Supermix (Bio-Rad Laboratories, Inc.) and 0.25 µmol/L each primer ...
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bioRxiv - Bioengineering 2020Quote: ... Equal volumes of 10 mg/L (GT)15- or (GT)6-Cy5-SWCNTs and 160 mg/L protein were added to a 96-well PCR plate (Bio-Rad) to a total volume of 50 μL ...
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bioRxiv - Microbiology 2021Quote: ... The soluble fraction was subjected to affinity chromatography at 4°C composed of 75 μL of amylose resin bound to 200 pmol of MS2-MBP protein in a Bio- Spin disposable chromatography columns (Bio-Rad). After washing ...
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bioRxiv - Bioengineering 2022Quote: ... Approximately 20 μL of the denatured phage was loaded in a 4-20 % gradient gel (Mini-PROTEAN® TGX Stain-Free gel, Biorad) and separated by electrophoresis at 120 V ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed thrice before the addition of horseradish peroxidase (HRP)-conjugated anti-mouse IgG (H+L) (Bio-rad, 170-6516) at 1:3,000 ...
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bioRxiv - Molecular Biology 2019Quote: ... 80 μL of the supernatant was removed (termed ‘whole cell extract [WCE] sample’) and 5 μg of antibody (anti-PK (Bio-Rad) or anti-HA (Roche) ...
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bioRxiv - Plant Biology 2021Quote: ... First-strand cDNA was synthesized in a 10-μl volume containing 350 ng of plant total RNA that was reverse transcribed employing an iScript cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... isolates with a meropenem minimum inhibitory concentration (MIC) greater than 0.125 mg/L had rapid phenotypic confirmation of carbapenemase production using the β-carba test (Bio-Rad). In addition ...
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bioRxiv - Immunology 2020Quote: ... A chemiluminescent signal visualized by Chemi-Lumi One L (07880-70, Nacalai Tesque) was detected with a ChemiDoc Touch imaging system (Bio-Rad) and analyzed with Image Lab software version 6.0.1 (Bio-Rad).
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bioRxiv - Cancer Biology 2019Quote: ... This 20-μL reaction mix was added to a DG8 cartridge together with 70 μL of Droplet Generation Oil for Probes (Bio-Rad) and used for droplet generation ...
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bioRxiv - Biophysics 2020Quote: ... the dynamics of PEN reactions were recorded in 20 μL solutions inside 150 μL qPCR tubes using a Qiagen Rotor-Gene qPCR machine or a CFX96 Touch Real-Time PCR Detection System (Bio-Rad). For biological relevance ...
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bioRxiv - Microbiology 2020Quote: ... Icecold freshly prepared competent cells (25 μl) were mixed with 4.5 μg of linear DNA and transferred to 0.1 cm-gap electroporation cuvettes (Bio-Rad, CA, USA). A pulse of 1.5 kV was applied to the cuvette with a MicroPulser electroporation apparatus (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... equipped with a diode array detector (L-7450) and separated on an AMINEX HPX-87H column (7.8 x 300 mm; BioRad, Munich, Germany) with a cation H guard pre-column ...
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bioRxiv - Neuroscience 2022Quote: ... at room temperature [goat anti-mouse 170-6516 or goat anti-rabbit 170-6515 IgG (H+L) HRP conjugate (Bio-Rad)] were all used at 1:2000 in 3 % BSA in 0.1 % Tween 20 (PBS/T) ...
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bioRxiv - Biophysics 2022Quote: ... 5 µL of the soluble fractions of cell lysates were run on a 4-20% TGX Mini-Protean gradient gel (Bio-Rad) and stained using Gelcode Blue (Thermo Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were detected by using horseradish peroxidase (HRP)-conjugated secondary antibodies (goat anti-mouse IgG (H+L)-HRP conjugate (1:10,000, Bio-Rad #1706516), goat anti-rabbit IgG (H+L)-HPR conjugate (1:10,000 ...
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bioRxiv - Microbiology 2022Quote: ... Plates were then washed six times with PBS-T and incubated with horseradish peroxidase-conjugated goat anti-rabbit IgG (H + L) (1:2000) (Bio-Rad) diluted in 2% horse serum/PBS for 1 hr at room temperature with agitation ...
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bioRxiv - Neuroscience 2022Quote: ... The pellet was resuspended in 50 μL 8M urea buffer (8 M urea, 100 mm NaHPO4, pH 8.5) and boiled in Laemmli sample buffer (BioRad, 161-0737) at 98°C for 5 min ...
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bioRxiv - Microbiology 2022Quote: LTA extracts (10 μl) were run on 15 % polyacrylamide gels and transferred to PVDF membranes using a Trans-Blot Turbo transfer system (Bio-Rad). After blocking with 5 % milk and 1 % BSA in TBST ...
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bioRxiv - Molecular Biology 2022Quote: ... The 10 µL cDNA reaction was diluted to 200 µL and 3 µL was used with iTaq Universal SYBR Green Supermix (Bio-Rad) and 670 nM of primer pairs (Table S4 ...
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bioRxiv - Microbiology 2023Quote: ... The qRT-PCR reaction was set up in a 20-μl volume with 100 - 200 ng of RNA and was conducted using the CFX Connect Real-Time PCR System (Bio-Rad).
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bioRxiv - Bioengineering 2023Quote: ... 1x DreamTaq buffer and 1 μL sample DNA in a total reaction volume of 10 μL using CFX96 Touch Real-Time PCR Detection System (BIO-RAD). The PCR amplification cycle was as follows ...
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bioRxiv - Immunology 2023Quote: ... and 100 μL of the secondary antibody solution - Goat Anti-Mouse IgG (H+L)-HRP Conjugate (Bio-Rad, Hercules, CA, USA) diluted in a 1:2,000 ratio in the antibody diluent - was then added and the plates were incubated for 1 hour in the dark at RT ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was then passed over 20 ml of Poly-L-proline conjugated beads in a disposable column (Bio-Rad, USA). The beads were first washed at room temperature in wash buffer (10 mM Tris pH 8 ...
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bioRxiv - Bioengineering 2023Quote: ... followed by goat anti-mouse IgG (H+L)-horseradish peroxidase (HRP)-linked secondary antibody (1:1000, 1721011, Bio-Rad, RRID: AB_11125936). Detection of the horseradish peroxidase signal was carried out using Clarity Western enhanced chemiluminescence (ECL ...
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bioRxiv - Immunology 2024Quote: ... 20 µl of each reaction mix and 70 µl of oil were converted to droplets with the QX200 droplet generator (Bio-Rad). Droplet-partitioned samples were then transferred to a 96-well plate ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was then passed over 20 ml of poly-L-proline conjugated beads in a disposable column (Bio-Rad, USA). The beads were first washed at room temperature in wash buffer (10 mM Tris pH 8 ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was then passed over 20 ml of Poly-L-proline conjugated beads in a disposable column (Bio-Rad, USA). The beads were first washed at room temperature in wash buffer (10 mM Tris pH 8 ...
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bioRxiv - Bioengineering 2023Quote: ... probed with a goat anti-mouse or goat anti-rabbit IgG H+ L-HRP conjugated secondary antibody (1:5000-1:10000) (Bio-Rad) for 1 hour ...
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bioRxiv - Microbiology 2024Quote: ... GFP-tagged proteins were detected using anti-GFP (Wako, mFX75, 1:5 000 dilution) antibodies combined with Goat Anti-Mouse IgG (H+L) HRP Conjugate (Bio-Rad). Images were acquired using Amersham Imager 600 (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... Gels were then stained with Coomassie Brilliant Blue G (80 mg dissolved in 1 L water with 30 mM HCl) and destained with water before visualising using a ChemiDoc MP Imaging System (Bio-Rad). For mNeonTP-loaded encapsulins ...
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bioRxiv - Bioengineering 2024Quote: ... 1x Dream Taq buffer A and 1 μL sample DNA in a total reaction volume of 10 uL using CFX96 Touch Real-Time PCR Detection System (BIO-RAD). The PCR amplification cycle was as follows ...
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bioRxiv - Cell Biology 2022Quote: Cytokine and growth factor secretion was measured with the Bio-Plex Pro Human Cytokine Screening Panel (Bio-Rad #M500KCAF0Y), a 27-plex assay ...
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bioRxiv - Neuroscience 2021Quote: ... The isolated placental CD34+ single cell suspension was incubated with human CD34- phycoerythrin (PE) (Bio-Rad; dilution 1:25), human CD45-FITC (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... Mono-Mac-6 effector cells previously stimulated with recombinant human IFN-ɣ (Bio-Rad, Hercules, CA, USA; cat. PHP050) at 0.2 µg ml-1 for 3h at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cytokine levels were measured using the magnetic bead-based Bio-Plex Pro Human cytokine 27-Plex (Bio-Rad) according to manufacturer’s instructions and measured the cytokines using the Bio-Plex 200 instrument ...
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bioRxiv - Microbiology 2023Quote: ... The samples were analyzed in duplicates using the Bio-Plex Pro™ Human Cytokine panel (Bio-Rad, Hercules, CA), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Cytokines were quantified in media isolated from MN cultures using the Bio-Plex Pro Human Cytokine kit (Biorad, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... smegmatis cells were transformed with 2 µL of the mycobacterial expression vector pNIT cloned with the gene Rv1630 (RpsA) using electroporation with a gene pluser (Bio-Rad, USA). Electrocompetent M ...
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bioRxiv - Molecular Biology 2019Quote: ... in a cartridge containing 20 µL of the reaction mix and 70 µL of Droplet Generation Oil for Probes (ddPCR™ 96-Well Plates #12001925, Bio-Rad). The entire emulsion volume was transferred from the cartridge to a 96-well PCR plate (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... membranes were washed 3x with TBS-T and incubated with either Goat Anti-Mouse IgG (H+L)-HRP Conjugate (Bio-Rad, #1721011) or Goat Anti-Rabbit IgG (H+L)-HRP Conjugate (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was washed three times for 10 minutes with 1x PBS and incubated with Goat Anti-Mouse IgG (H+L)-HRP Conjugate (BioRad, #172-1011). Chemiluminescence was induced by incubation with SuperSignal West Femto Maximum Sensitivity Substrate (ThermoFisher ...
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bioRxiv - Biophysics 2022Quote: ... TMEM87A in buffer 1 was exchanged into soybean l-α-phosphatidylcholine (Soy PC, MillaporeSigma) with the addition of Biobeads SM2 (Bio-Rad) and an hour incubation at a protein:lipid ratio of 1:10 (corresponding to 0.4 mg purified TMEM87A and 4 mg of Soy PC lipid or 1:50 in buffer (5 mM HEPES pH 7.2 ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was soaked with Chemi-Lumi One L or Chemi-Lumi One Super (Nacalai Tesque) for the signal detection using ChemiDoc XRS+ (Bio-Rad Laboratories).
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bioRxiv - Microbiology 2022Quote: ... Milli-Q water was treated with Chelex 100 resin 100–200 mesh sodium form via batch method (50 g/L, Bio-Rad Laboratories). A concentrated stock of SD media not containing Cu2+ ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein samples were combined with the 4x Laemli sample buffer (900 μl of sample buffer and 100 μl β-Mercaptoethanol), heated (95°C, 5mins) and run on SDS-PAGE (protean TGX pre-casted gradient gel, 4%-20%, Bio-rad) and transferred to the Nitro-Cellulose membrane (Bio-Rad ...
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bioRxiv - Plant Biology 2021Quote: ... 20 μl of eluted protein samples were separated on SDS gel (12%) and transferred to PVDF membrane (Immun-Blot® PVDF, Bio-Rad) through semi-wet blotting protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μL of cDNA (10 ng of starting RNA) was amplified per reaction using the iTaq Universal SYBR Green Supermix (Bio-Rad, #1725124) and the Bio-Rad CFX96 qPCR system.