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Citations for Bio-Rad :
351 - 400 of 459 citations for L Phenylalanine N T Boc 13C9 97 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... versions with an N-terminal cysteine were additionally made in order to conjugate a TMR-C5-maleimide Bodipy dye (Bio-Rad, CA, USA), to make fluorescent TMR-C5-maleimide-SPTISITAPIDVLRKTWAKENMRKQMQINREYLKNLQamide (DH37-F ...
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bioRxiv - Microbiology 2021Quote: ... 40 μg of ΔORF3-E mNG RNA and 20 μg of N-gene RNA were electroporated into 8×106 Vero-ORF3-E cells using the Gene Pulser XCell electroporation system (Bio-Rad, Hercules, CA) at a setting of 270V and 950 μF with a single pulse ...
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bioRxiv - Biochemistry 2020Quote: ... Total RNA (1 µg) from each sample sets (n=3) was taken for cDNA preparation using iScript™ cDNA Synthesis Kit (Bio-Rad, USA). Real-Time qPCR was performed using PowerUp SYBR Green Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2023Quote: Rabies virus N gene was amplified by real-time quantitative PCR using iTaq™ Universal SYBR® Green One-Step Kit (BIO-RAD). The primers are as follow ...
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bioRxiv - Immunology 2019Quote: ... we measured bacterial growth by detection of luminescence at λ = 470 using a Spectramax L luminometer (Bio-Rad) or by dilution of infected cultures on BYCE agar plates for enumeration of CFU ...
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bioRxiv - Cell Biology 2019Quote: ... 5 min) and incubated for 1 h at room temperature with goat anti-rabbit IgG (H+L) (BioRad). Membranes were washed and signal was detected using the ChemiDoc XRS+ (Biorad ...
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bioRxiv - Neuroscience 2022Quote: Goat anti-mouse IgG (H+L) horseradish peroxidase-conjugated secondary antibody (Bio-Rad, 1:5000; Cat. 170-6516,) and Goat anti-rabbit Igg (H+L ...
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bioRxiv - Plant Biology 2022Quote: ... For α-ACTIN-2 the secondary antibody Goat Anti-Mouse IgG (H + L)-HRP conjugate (1706516, Bio-rad) was used at a dilution of 1:5000 ...
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bioRxiv - Immunology 2023Quote: ... unreacted ITC-DTPA was removed by dialysis against 0.25 M ammonium acetate (NH4Ac) pH 5.4–5.5 (metal free) supplemented with 2 g/L Chelex (Bio-Rad) using 20,000 molecular weight cut-off dialysis cassettes (Slide-a-Lyzer ...
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bioRxiv - Biophysics 2022Quote: ... then 6 L of RO water with Ca2+ chelating beads (BIO-RAD Chelex 100 Resin Cat#142-1253), and finally 6 L of RO water alone ...
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bioRxiv - Microbiology 2023Quote: ... folic acid 500 μg/L) or on Columbia agar solid support enriched with 5% horse blood (COH) (Biorad). Anoxic conditions were generated in Gaspack (BD ...
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bioRxiv - Cell Biology 2023Quote: ... the membranes were incubated with goat anti-rabbit IgG (H+L chains) HRP-conjugated secondary antibodies (Bio-Rad) and detected using SuperSignal West Pico ECL (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were incubated in 1:5,000 goat anti-mouse IgG (H+L)-HRP (Bio-Rad, catalog number 1706516) in 6% milk in PBS-T ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 g SDS in 1 L distilled water) using the Mini-PROTEAN® Tetra electrophoresis system (Bio-Rad). Then ...
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bioRxiv - Immunology 2024Quote: ... SDS 10g/L) and transferred onto a PVDF membrane with the Trans-Blot Turbo Transfer System (Bio-Rad). The membrane was blocked for 2h with a blocking solution (TBS ...
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bioRxiv - Systems Biology 2021Quote: ... which contained 200 nM of TSWV-N or thrips internal reference Actin gene primers in the SYBR Green Supermix (Bio-Rad, Hercules, CA, USA). Samples with missing and/or high (>35 ...
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bioRxiv - Microbiology 2023Quote: ... ddPCRs for each sample (n=50) were prepared in an initial volume of 72 µl comprising 37.5 µl EvaGreen Supermix (QX200; Bio-Rad, South Granville, NSW, Australia), 31.5 µl sterile water ...
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bioRxiv - Plant Biology 2019Quote: ... the membrane was incubated with the secondary antibody: the goat antirabbit IgG (H + L)-horseradish peroxidase conjugate (Bio-Rad). Blots were visualized by an Amersham Imager 600 gel scanner (GE HealthCare Life Sciences ...
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bioRxiv - Biochemistry 2020Quote: ... The plate was then washed two times with TBST and then incubated with a 1:5,000 dilution of secondary antibody (Goat Anti-Rabbit IgG (H+L)-HRP (BioRad)) for 1 hour ...
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bioRxiv - Biochemistry 2020Quote: ... The plate was then washed with TBST and then incubated with a 1:10,000 dilution of secondary antibody (Goat Anti-Rabbit IgG (H+L)-HRP (BioRad)) for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... PCR reactions were carried out in a final volume of 20 ◻ l reactions containing iQ Supermix (Bio-Rad), 5 nM FITC dye ...
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bioRxiv - Genetics 2019Quote: ... 10 mM L-serine) to a UapA concentration of 20 μM using Micro Bio-Spin 6 columns (Bio-Rad). UapA was loaded into gold coated capillaries and the protein sprayed into a Synapt G2-Si (Waters ...
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bioRxiv - Microbiology 2021Quote: ... SOB motility plates (containing 10-4 M IPTG and 2.4 g/L MgSO4) at 37°C and pictures were taken using a Gel Doc (Biorad) imager at the beginning and the end of the linear swimming motility period (from 5 to 8 hours) ...
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bioRxiv - Neuroscience 2022Quote: ... and Goat anti-rabbit Igg (H+L) horseradish peroxidase-conjugated secondary antibody (Bio-Rad, 1:5000; Cat. 170-6515,) were used as secondary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... IgM-containing Capto L fractions were pooled and subjected to cation exchange chromatography using Nuvia HR-S (Bio-Rad) with an equilibrating and wash buffer (20 mM acetate ...
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bioRxiv - Cell Biology 2023Quote: ... secondary antibodies (IgG(H+L)-HRP) were used at 1:10000 dilution for detection using a Versadoc system (Biorad).
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bioRxiv - Bioengineering 2020Quote: ... A 10 μL cell-free sample was injected into a BioRad chromatographic column (Aminex HPX-87H, 7.8 mm 300 mm, Biorad, USA). The HPLC was equipped with a P680 pump ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesized using 600 ng input total RNA per 40 ¼l reaction with the iScript cDNA Synthesis Kit (BioRad), cycling for 5 min at 25°C ...
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bioRxiv - Molecular Biology 2019Quote: ... and goat anti-rabbit IgG H&L HRP conjugate at a dilution of 1:2000 (Bio-Rad, Hercules, CA, USA) was used as the secondary antibody ...
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bioRxiv - Biochemistry 2020Quote: ... proteins were dialyzed in HEPES sodium salt-HCl (75 mM, pH 7.0) with Chelex-100 resin (5 g/L, Bio-Rad) to remove any divalent metal contaminants ...
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bioRxiv - Neuroscience 2020Quote: ... Goat anti-mouse IgG (H+L) horseradish peroxidase-conjugated secondary antibody (1: 8000; Cat. 170-6516, Bio-Rad, Milan, Italy) was used ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... The 20 μL reaction mix contained 5 μL sample or standard DNA together with 10 μL 2x SsoAdvanced Universal Probes Supermix (Biorad), 1 μL of each primer (5 μM stock solution) ...
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bioRxiv - Microbiology 2020Quote: ... 5 μl of the supernatant was used to normalize the level of total protein in each sample via BCA assay (BioRad) and the supernatant was then flash frozen on dry ice ...
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bioRxiv - Biophysics 2021Quote: ... folding was quenched by taking 10 μL of sample and adding it to 10 μL of 2x Laemmli SDS gel-loading buffer without reducing agent (BioRad), vortexed and stored at 4°C until analysis ...
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bioRxiv - Genetics 2022Quote: ... followed by goat anti-mouse IgG (H+L) alkaline phosphatase (AP)-conjugated (1:3000, Bio-Rad Cat# 170-6520, RRID:AB_11125348) antibody or Alexa Fluor 546 goat anti-mouse IgG (H+L ...
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bioRxiv - Bioengineering 2019Quote: ... Triplicate PCR reactions were performed in 20 µl final volume with 10 ng of fragmented gDNA using the ddPCR Supermix for Probes (No dUTP) master mix (Biorad) with 11-11 pmol primers and 50-50 pmol of TaqMan probes (for sequences of primers and probes see Table 1 below) ...
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bioRxiv - Molecular Biology 2019Quote: ... Then the membranes were incubated with goat anti-rabbit IgG (H+L) alkaline phosphatase conjugate (1:10,000 dilution) (BIO-RAD). Subsequently ...
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bioRxiv - Bioengineering 2020Quote: 50 μL of PCR mastermix solution in Nuclease-free ultrapure water contained 60 mM Tris-HCl Buffer pH 8.8 (Bio-Rad), 200 μM dNTPs ...
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bioRxiv - Immunology 2021Quote: ... 200 µL of bead mix solution were added to all wells and washed using a Bio-Plex Pro Wash Station (BIORAD). 25 µL of Universal Assay Buffer were added to all wells ...
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bioRxiv - Developmental Biology 2020Quote: ... 5 μL of PCR1 reaction (2 ng) were combined for the qPCR reaction using CFX 96 Touch Biorad qPCR thermocycler (Biorad). Δct was calculated by subtracting the mean Cq of each sgRNA to a non-targeting control ...
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bioRxiv - Biophysics 2022Quote: ... 2 μl of recombinant proteins and 1 μl of a molecular weight marker (Precision Plus Protein Kaleidoscope Prestained Protein Standards, BioRad) were applied to the PAGE lane (10 wells ...
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bioRxiv - Molecular Biology 2023Quote: ... 25 μl of the sample were heated at 95°C for 5 min and resuspended in 4x Laemmli sample buffer (BioRad)/50 mM DTT before SDS-PAGE.
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bioRxiv - Microbiology 2023Quote: ... followed by three-times washing with TBST for 5 min and subsequent incubation with the goat anti-rabbit IgG (H+L)-HRP conjugate (BioRad) (diluted 1:3000 in TBST ...
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bioRxiv - Microbiology 2024Quote: ... Plates were washed three times before the addition of HRP-conjugated anti-mouse IgG (H+L; Bio-Rad; 170-6516) at 1:3,000 and anti-mouse IgG2b (ab97250 ...
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bioRxiv - Bioengineering 2024Quote: ... Fully expanded leaves from in vitro cultured Nicotiana tabacum plants (L. cv. Petite Havana) were bombarded with 50 μg of 0.6-μm gold particles (Bio-Rad) coated with 10 μg of plasmid DNA using 1,100 psi rupture discs (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... Blocking of the membrane was carried out by incubating in blocking solution for 1 h at room temperature (5% Bio-Rad Blotting-Grade Blocker in TSB-T buffer). Membrane was then incubated with anti-PcdA polyclonal antibody diluted 1:15,000 in blocking solution for 2 h at room temperature.
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bioRxiv - Cell Biology 2020Quote: ... 2 mM L-glutamine and counted in the presence of Trypan Blue using the TC20™ Automated Cell counter (Bio-Rad) (24h floating cell count) ...
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: ... 1.5 μl for bound franction for GFP detection) were loaded on SDS–PAGE (4-20% Mini-PROTEAN TGX Stain-Free, Bio-Rad) and analyzed by Western blot.
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bioRxiv - Molecular Biology 2021Quote: Samples in 1x Laemmli loading buffer (generally equivalent to 1 µL replication reaction or chromatin recovered from 8 µL plasmid pull-down) were resolved on 10% or 4-15% acrylamide Mini-PROTEAN or Criterion TGX precast gels (Bio-Rad) and transferred to PVDF membranes (Perkin Elmer) ...
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bioRxiv - Immunology 2021Quote: ... 1/10 dilutions were used in triplicates with 0.2 μM primer and 10 μL LightCycler 480 SYBR Green I Master in a 20 μL reaction and qPCR executed in a 98- well block on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad). Absolute transcript levels for TDP-43 ...