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Citations for Bio-Rad :
101 - 150 of 348 citations for IL 9 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... His-tagged proteins in the cleared lysate were purified using Profinity™ IMAC Nickel Charged Resin (Bio-Rad) and eluted in 50 mM sodium phosphate buffer with 300 mM NaCl pH 8 and 300 mM imidazole (Fischer Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... monoclonal rat anti-human CD3 (Bio-Rad), rat anti-mouse B220/CD45R (clone RA3-6B2 ...
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bioRxiv - Immunology 2023Quote: ... goat-anti-human 1:3000 (Biorad /1721050); goat-anti-rabbit 1:2000 (CST / 7074) ...
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bioRxiv - Bioengineering 2020Quote: ... EG depletion was monitored using on an Aminex HPX-87H ion exclusion column (300 mm x 7.8 mm, particle size 9 μm; Bio-rad). The column was maintained at 40°C and samples were isocratically eluted using 0.014 N H2SO4 at a flow rate of 0.55 ml min-1 and read on a refractive index detector (RID) ...
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bioRxiv - Immunology 2022Quote: ... Lysates containing 40 μg of proteins were analyzed by SDS-PAGE on 9-13% gels and proteins were transferred onto nitrocellulose membranes (BioRad). The blots were blocked in non-fat milk diluted in tris-buffered saline (TBS ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was isolated from 6-week-old rosette leaves and bolting flower buds of Col-0 and one of the RPF2-atp1 (RPF2-atp1-9) transformed lines (T3) with PureZol reagent (BioRad). Three independent libraries for each genotype were made from total RNA treated with 250 ng of Turbo DNase (Ambion ...
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bioRxiv - Synthetic Biology 2022Quote: ... All reagents except the double-stranded beacon were first assembled in a total volume of 9 μL and run at 37 °C in a CFX96 real-time PCR detection system (Biorad). Both WT SpCas9 and SpCas9-NG are highly active at this temperature ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was extracted using PureZOL reagent (Bio-Rad Laboratories, Des Plaines, IL, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The plate was subsequently loaded into the QX200 Plate Reader (Bio-Rad, IL USA) and data was analysed using the QuantaSoft Software (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... run on a CFX96 Real-Time System (Bio-Rad Laboratories, Des Plaines, IL, USA). Relative mRNA expression of each gene of interest (GOI ...
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bioRxiv - Bioengineering 2021Quote: ... Membranes were stained overnight at 4°C with a 1:1000 diluted mouse anti-his primary antibody (MCA1396, RRID:AB_322084, Bio-Rad) and then for 1 hour at room temperature with a 1:4000 diluted rabbit anti-mouse HRP secondary antibody (SouthernBiotech Cat# 6170-05 ...
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bioRxiv - Molecular Biology 2023Quote: ... His-tagged recombinant protein was purified using Ni-NTA purification with the help of Ni-charged resins (Bio-Rad). Purified protein was further used for kinase assay ...
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bioRxiv - Immunology 2021Quote: ... sections were stained with 0.4 µg/mL rabbit -anti-human CD20 polyclonal antibodies (Neomarkers) and 2 µg/mL rat-anti-human CD3 antibodies (clone MCA1477, BioRad). Then ...
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bioRxiv - Microbiology 2020Quote: ELISA plates were coated overnight at 4°C with 0.1 µg/mL of mouse anti-human IgG (human CH2 domain with no cross-reactivity to rhesus macaque IgG; clone R10Z8E9; BioRad) and then blocked for 2 hours ...
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bioRxiv - Immunology 2023Quote: ... IL-17A titres in human serum samples were quantified using a multiplex cytokine panel (Bio-Plex Pro Human Cytokine Assay, BioRad) and a LuminexCorp Luminex 100 machine as per the manufacturer’s instructions.
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bioRxiv - Genomics 2020Quote: ... were seeded per well in 6-well plates (2 ml per well). 24 hrs later cells were transfected with siRNA (Supp. Table 9) using SilentFect (Bio-Rad) in biological triplicates ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were replenished with compounds every 24 h and counted on days 1-9 using automated cell counter (Bio-Rad). KYSE-520 cells were plated onto 96-well plates (500 cells per well ...
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bioRxiv - Biochemistry 2021Quote: ... RQN > 9) were prepared using the NEBNext Ultra II Directional RNA Library Kit for Illumina with NEBNext rRNA depletion (Bio-Rad). Protocols were followed as per the manufacturer’s guidelines except that 40X adaptor dilutions were utilized ...
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bioRxiv - Microbiology 2021Quote: ... Sugar consumption and the metabolite production were analyzed using HPLC Prominence (Shimadzu) with ion exclusion column Aminex ®HPX-87H (300×7.8mm×9 μm) (Bio-RAD), isocratically eluted at 60 °C with 5 mM sulfuric acid flow rate of 0.6 mL.min-1 ...
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bioRxiv - Cancer Biology 2022Quote: ... The immunoprecipitated proteins from three treatments were mixed and either denatured by 9 M Urea/20 mM HEPES buffer or mixed with 2x Laemmli Sample Buffer (1610737, Bio-Rad) and separated by SDS-PAGE to analyze individual fractions ...
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bioRxiv - Physiology 2023Quote: ... Samples were run 16 h at 9 °C at 4 V with a 1–10 running ratio in TBE buffer using CHEF-DRII system (Bio-Rad). After the run ...
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bioRxiv - Genomics 2023Quote: Pooled blood cells from 9 adults were counted and their viability was assessed using an automatic cell counter (Bio-Rad TC20) with Trypan Blue staining ...
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bioRxiv - Genetics 2023Quote: ... China) using TBE (65 mM Tris-HCl, 27 mM boric acid, 1 mM EDTA, pH 9; Bio-Rad, Hercules, CA, USA) as the buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was generated by reverse transcription of up to 9 ng RNA from each sample using iScript Reverse Transcription Supermix for RT-qPCR (BioRad, 1708841). mRNA expression was determined on a StepOnePlusTM Real-Time PCR System (Thermo Fisher ...
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Tracing production instability in a clonally-derived CHO cell line using single cell transcriptomicsbioRxiv - Cell Biology 2021Quote: ... The recombinant Human IgG1 Kappa (Biorad, cat.no. HCA192) was used as a positive control ...
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bioRxiv - Microbiology 2019Quote: ... anti–human IgG conjugated with HRP (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2021Quote: ... anti-human IgG-HRP (Bio-Rad 172-1033) and anti-Rat IgG-HRP (Thermo Fisher 31470).
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bioRxiv - Cancer Biology 2023Quote: ... Human GAD1 PrimePCR primers were purchased from BioRad and the sequence for human GAPDH primers are as follows ...
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bioRxiv - Neuroscience 2024Quote: The human cytokine Luminex 8-plex assay (Biorad) was utilised to measure the concentration of IFNγ ...
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bioRxiv - Genetics 2021Quote: ... RNA was reverse-transcribed using an iScript cDNA synthesis kit (Bio-Rad, Des Plaines, IL). Assay of RNA via quantitative PCR [qPCR] was performed with iTaq universal SYBR green supermix (Bio-Rad) ...
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bioRxiv - Cancer Biology 2022Quote: ... qPCR was conducted using iQ SYBR green supermix (Biorad, cat# 1708880, Des Plaines, IL, USA) and Bio-Rad CFX96 Fast Real-Time PCR Systems ...
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bioRxiv - Biochemistry 2022Quote: ... The refolded mature FAM237A was eluted from the C4 reverse-phase column (Hi-Pore reversed-phase column, 4.6 × 250 mm, Bio-Rad) by the acidic acetonitrile gradient.
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bioRxiv - Microbiology 2023Quote: ... The was filtered through a 0.45 μm filter and His-MSI-2 was purified with HisTrapTM FF column (Cytiva) using the Econo Gradient Pump (BIORAD) according to the manufacturer’s protocol and overnight dialysis ...
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bioRxiv - Microbiology 2023Quote: ... Ni-NTA agarose with the bound His-SUMO-SigN was loaded on a Poly-Prep® Chromatography Column (Bio-Rad), washed with P2 buffer and subsequently with the P2 buffer with the 30 mM imidazole ...
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bioRxiv - Molecular Biology 2019Quote: ... Denatured protein extracts were resolved using 4-9% SDS-PAGE (4–15% Mini-PROTEAN® TGX™ Precast Protein Gelscat# 4561083, Biorad) and then transferred to a nitrocellulose membrane.
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bioRxiv - Cancer Biology 2020Quote: ... a ddPCR reaction was prepared (13 μl mastermix and 9 μl cfDNA) and subsequently ran on a QX200 ddPCR system according to protocol (Bio-Rad Laboratories). Data analysis was performed using QuantaSoft v1.7.4.0917 (Bio-Rad Laboratories) ...
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bioRxiv - Molecular Biology 2019Quote: ... Denatured proteins (20-40 µg) were electrophoresed in 9% SDS-PAGE gels or MiniPROTEAN® TGX™ 4-15% Precast gels (BIORAD), transferred onto a nitrocellulose membrane and probed with specific antibodies ...
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bioRxiv - Plant Biology 2019Quote: ... A Bio-Rad Aminex HPX-87H (300 x 7.8 mm, 9 μm particle size) column with a Micro-Guard Cation H guard column (Bio-Rad, Watford, UK) was used to achieve separation with an isocratic gradient of 5 mM sulfuric acid ...
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bioRxiv - Cancer Biology 2021Quote: ... Ribonucleoprotein (RNP) complexes were made at a 9:1 ratio of sgRNA:Cas9 (90pmol:10pmol) in Gene Pulser® Electroporation Buffer (Bio-Rad, 1652677) and incubated for 10 min at RT ...
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rpoB, a promising marker for analyzing the diversity of bacterial communities by amplicon sequencingbioRxiv - Microbiology 2019Quote: ... The 16S rRNA gene was amplified (the primer sequences are indicated in Additional file 9) in a Bio-Rad thermocycler (Bio-Rad, USA). PCR products were analyzed by agarose gel electrophoresis ...
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bioRxiv - Developmental Biology 2019Quote: ... using 9% SDS polyacrylamide gels and then transferred to a 0.2µM polyvinylidene fluoride (PVDF) membrane over 10 minutes (Bio-Rad Trans-Blot Turbo). Membranes were blocked with 5% skim milk in 0.05% TBS-T for 1 hour ...
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bioRxiv - Physiology 2023Quote: ... 0.03% bromophenol blue) containing 9% beta-mercaptoethanol and separated by Mini-Protean TGX gel 4 - 15% (BioRad, stain-free gels #4568084, 4561035) and blotted onto Amersham Hybond-P PVDF membrane ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Protein lysates were boiled in loading buffer [1:9 ratio of 2-mercaptoethanol:4x Laemmli Sample Buffer (Cat.#1610747; Bio-Rad Laboratories)] for 10 min ...
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bioRxiv - Developmental Biology 2020Quote: Levels of PAI1 in human plasma were measured using a Bio-Plex multiplex enzyme immunoassay (Customized Human Cancer Biomarker Assay, Bio-Rad Laboratories). Plasma was diluted 1:4 in assay diluent prior to performing the assay ...
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bioRxiv - Microbiology 2020Quote: ... Cell culture supernatants were screened for the presence of 27 human cytokines and chemokines using the Bio-Plex Pro Human Cytokine Standard 27-Plex kit (Bio-Rad Laboratory) on a FLEXMAP 3D® analyzer (Luminex ...
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bioRxiv - Biochemistry 2023Quote: ... The culture medium after incubation was analyzed for the presence of secreted human cytokines using Bio-Plex Pro Human Cytokine 17-plex Assay (Bio-Rad, USA) and Bio-Plex 200 Systems (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... Conjugates against anti-human IgG-HRP (BioRad, Richmond, CA) or anti-human IgA-HRP (Nordic BioSite ...
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bioRxiv - Cancer Biology 2020Quote: ... with certified human GAPDH and LOX primers (Bio-Rad). The reaction was run in CFX Connect 96 Real Time PCR system (Bio-Rad) ...
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bioRxiv - Cancer Biology 2021Quote: ... Bio-Plex Pro Total Human GAPDH (BioRad, USA, 171V60019M), Bio-Plex Pro Total β-Actin (BioRad ...
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bioRxiv - Molecular Biology 2022Quote: ... 1× AP3B1-HEX-labelled human reference assay (dHsaCP1000001, BioRAD), 1/40 HindIII (Thermo Fisher Scientific ...