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Citations for Bio-Rad :
1 - 50 of 3162 citations for Human PDGF alpha receptor PDGFRA qPCR Primer Pair since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The following primer pairs were used to carry out qPCR reactions in a CFX96 Real Time System (BioRad) using SYBR Green (BioRad):
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bioRxiv - Microbiology 2020Quote: ... 10.5 μl template DNA and 1 μl respective primer pairs (200 nM final concentration, supplementary table 1) were mixed and distributed into a 96-well qPCR plate (Bio-Rad). The plate was incubated 10 min at room temperature to digest the target DNA before droplets were generated in an Automated Droplet Generator (Bio-Rad) ...
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bioRxiv - Bioengineering 2021Quote: ... Commercial primer pairs were used for GJA1 (Bio-Rad, qHsaCID0012977), as well as B2M (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... mixed with primer pairs for each gene and SYBRgreen (BioRad). All primer pairs were designed using Primer3 with spliced sequences retrieved from Wormbase (wormbase.org ...
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bioRxiv - Immunology 2022Quote: ... and 1 µl of respective primer pairs (200 nM final concentration) were mixed and distributed into 96-well qPCR plate (Bio-Rad; cat. #12001925). Droplets were generated in an Automated Droplet Generator (Bio-Rad) ...
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bioRxiv - Cancer Biology 2022Quote: ... qPCR was performed using human-specific primers (Table S1) and iQ SYBR Green Supermix (Bio-Rad), with annealing temperature optimized by gradient PCR ...
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bioRxiv - Biochemistry 2022Quote: ... For RT-qPCR, cDNA was amplified using 150 nM gene-specific primers (IDT, PrimeTime qPCR primers) with SsoFast EvaGreen Supermix (Bio-Rad) on a Bio-Rad CFX96 real-time PCR detection system ...
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bioRxiv - Neuroscience 2021Quote: ... SYBR Green intron-spanning pre-designed qPCR primers (BioRad) were used ...
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bioRxiv - Physiology 2024Quote: ... and 100mM primers using CFX96 qPCR machine (Bio-Rad, Hercules ...
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bioRxiv - Bioengineering 2023Quote: ... All primers were qPCR Prime PCR Assays (Bio-Rad), and the unique IDs for each primer set are provided in Supplemental Table 1 ...
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bioRxiv - Molecular Biology 2020Quote: ... primer pairs (see Table S5) were mixed with cDNA and iQ SYBR’Green Supermix (Bio-Rad), and qRT-PCR was run on an iQs Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Physiology 2022Quote: ... and 100nM primers at a CFX96 qPCR machine (Bio-Rad, Hercules ...
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bioRxiv - Physiology 2023Quote: ... P2Y2R qPCR primers were obtained from Bio-Rad (Hercules, CA). Results were analyzed using the ΔΔCt method.
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bioRxiv - Molecular Biology 2023Quote: ... and subsequently qPCR-amplified using SYBR Primer Assay kits (Biorad). Notably ...
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bioRxiv - Physiology 2023Quote: ... and 100nM primers at a CFX96 qPCR machine (Bio-Rad, Hercules ...
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bioRxiv - Cancer Biology 2023Quote: ... Human GAD1 PrimePCR primers were purchased from BioRad and the sequence for human GAPDH primers are as follows ...
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bioRxiv - Cancer Biology 2020Quote: ... and a desired primer pair on the CFX Connect™ Real-Time PCR Detection System (Bio-Rad). The cycling conditions used were ...
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bioRxiv - Cancer Biology 2020Quote: ... with certified human GAPDH and LOX primers (Bio-Rad). The reaction was run in CFX Connect 96 Real Time PCR system (Bio-Rad) ...
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bioRxiv - Molecular Biology 2022Quote: ... Angel1 qPCR was carried out using pre-validated primers (Bio-Rad, 10025636). All other qPCR primer pairs are listed in Supplementary Table S2.
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bioRxiv - Biochemistry 2021Quote: ... Real-time PCR was then performed with each unique primer pair according to manufacturer’s protocols (iTaq Universal SYBR Green Supermix, BioRad) to quantify undigested (that is ...
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bioRxiv - Microbiology 2022Quote: ... using a standard protocol from the manufacturer and this pair of primers in a S1000 thermal cycler (Bio-Rad). Expression and purification of FimT3s-R160AR162A were performed as described above (see SI Materials and Methods ...
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bioRxiv - Developmental Biology 2021Quote: ... and a relative quantification of specific genes was performed in a Bio-Rad qCycler using either TaqMan probes in iTaq Universal Probes Supermix or specific primer pairs in iTaq Universal SYBR Green Supermix (BioRad). Probes and Primers used are listed in Suppl ...
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bioRxiv - Plant Biology 2023Quote: ... a standard curve was generated to estimate the PCR efficiency of each primer pair using CFX Maestro software v.2.0 (Bio-Rad). The slope and coefficient of determination (R2 ...
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bioRxiv - Biochemistry 2021Quote: ... Human primers were designed by using Beacon Designer software (Bio-Rad). Quantitative PCR was performed on a CFX96 real-time PCR thermocycler (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... Primers/probes to detect human RPP30 (BioRad Custom Assay, Hercules, CA) were used as an endogenous reference in human samples ...
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bioRxiv - Genetics 2021Quote: ... supplemented with gene-specific primer pairs (Supplementary Table S10) on a CFX Connect Real-Time PCR Detection System (Bio-Rad Laboratories). The ACTB transcript was used for the normalization of the target gene.
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bioRxiv - Plant Biology 2021Quote: ... Quantitative real-time PCR was performed in triplicates using 10-20 ng cDNA as template and gene specific primer pairs shown in Table S8 in CFX Connect Real-Time PCR System (Bio-Rad). The PCR conditions were 95◻°C for 15◻min ...
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bioRxiv - Molecular Biology 2020Quote: ... the primer pairs (Table 2; exception: S. umbla) were used to create EvaGreen-based droplet dPCR assays using the AutoDG (Bio-Rad) for droplet generation ...
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bioRxiv - Biochemistry 2024Quote: ... and gene-specific primer pairs (Table 4) on the CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA). The mRNA levels were normalized with respect to Gapdh.
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bioRxiv - Bioengineering 2024Quote: ... qPCR was performed with TaqMan Fast Advance Mastermix and primer pairs listed in Supplementary Table 2 and analysed using a CFX96TM Real-Time PCR System (Bio-RAD). Gene expression was normalised to glyceraldehyde 3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Immunology 2021Quote: ... specific for the various human chemokine receptor genes and IQ™ SYBR green reagent (# 170882, BIO-RAD) on CFX96 real-time PCR detection system (BIO-RAD) ...
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bioRxiv - Immunology 2022Quote: ... Pre-designed human gene primers were purchased from Bio-Rad (supplemental Table). Human PPIA was amplified in parallel and used as the reference gene in quantification ...
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bioRxiv - Cancer Biology 2022Quote: ... Human primers for HLA-DRB1 and VCAM1 were purchased from Bio-Rad.
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bioRxiv - Physiology 2020Quote: ... Gene expression was assessed using commercially available Taqman primer/probe sets (Thermo Fischer) or specific oligonucleotide pairs (Table 1) and SYBR® Green supermix (Bio-Rad). Relative expression of the target genes was calculated with the comparative Ct method [43] using glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Neuroscience 2021Quote: ... Master mixes containing primer pairs against FIBCD1 or B2M (Beta-2-microglobulin, housekeeping gene) were prepared with iTaq Universal SYBR Green Supermix (Bio-Rad #1725122). The mastermixes were dispensed into each well containing cDNA and incubated on ice for 15 minutes to allow the cDNA to dissolve ...
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bioRxiv - Developmental Biology 2021Quote: ... following by 6 cycles of pre-amplification with pooled 47 pairs of MTE primers designed for NanoString CodeSet (SsoAdvanced™ PreAmp Supermix, Cat# 1725160, Bio-Rad); amplified dsDNA was analysed using the NanoString expression protocol.
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bioRxiv - Immunology 2024Quote: ... RT-qPCR was conducted using HiScript II One Step qRT-PCR SYBR Green Kit (Q221-01, Vazyme) with gene-specific primer pairs on a CFX Connect Real-Time PCR Detection System (Bio-Rad Laboratories). The thermal cycling protocol was as follow ...
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bioRxiv - Plant Biology 2023Quote: ... Transcript abundance in cDNA dilutions of 1/100 was measured in 25 µl reactions using qPCRBIO SyGreen LO-ROX RT-qPCR reagent (PCR Biosystems) with relevant primers on a CFX96 qPCR machine (Bio-Rad, California, US). Transcript abundance was normalized to TaHK4 (TraesCS5A02G015600 ...
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bioRxiv - Neuroscience 2020Quote: ... and assessed using qPCR with optimized primers and SsoAdvanced SYBR Green Master Mix (BIO-RAD). Cycle time (Ct ...
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bioRxiv - Cell Biology 2023Quote: ... qPCR was performed using primers (Table S1) and Sso Advanced Universal SYBR Green Supermix (BioRad). Samples were processed as technical triplicates with the number of independent experimental replicates reported in the figure legends ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Final qPCR was performed using TLR4 (qMmuCIP0035732) and HPRT (qMmuCEP0054164) primer-probe assays obtained from BioRad and the associated SSO Advanced Universal Probe Super Mix kit (BioRad 1725281).
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bioRxiv - Cancer Biology 2020Quote: ... using gene-specific primers for stem-loop qPCR or the iScript cDNA Synthesis Kit (BioRad, 1708890). cDNA was analyzed using SYBR Green Master Mix (BioRad ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA abundance was measured using 300nM of indicated primers and a iQ5 qPCR system (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative PCR (qPCR) was performed with primer sets corresponding to primer list (Table 3) and using iTaq Universal SYBR Green Supermix (Bio-Rad, Cat. No. 172-5125) on an Applied Biosystems 7500 Fast Real-Time PCR system or ThermoFisher Scientific QuantStudio 3 or 5 Real-Time PCR system ...
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bioRxiv - Molecular Biology 2021Quote: ... 300nM final volume of each forward and reverse primer pair, 5 μL SYBR Green Supermix (50% total volume of reaction, rxn) (Bio-Rad Inc. Hercules, CA), and nuclease-free water (to make a final reaction volume of 10 μL) ...
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bioRxiv - Cell Biology 2020Quote: ... For qPCR the primers listed in the Supplementary Information were used with iQ Sybr Green (Bio-Rad). Fold change in RNA expression was determined by the ΔΔCt method with normalization to PSMA5 ...
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bioRxiv - Cancer Biology 2021Quote: ... quantitative PCR (qPCR) was performed using 500 nM primers and SsoAdvanced Universal SYBR Green Supermix (Bio-Rad). The reaction conditions consisted of a 30-s 95°C enzyme-activation cycle ...
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bioRxiv - Microbiology 2021Quote: ... assay primers and cDNA were prepared in 20 uL reaction and run on CFX96 qPCR machine (Bio-Rad). Zika RNA levels were measured by RT-qPCR and either normalized to GAPDH or calculated to copy number from an external standard with ΔΔCt method ...
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bioRxiv - Physiology 2024Quote: ... All primers used in this study were mRNA specific and designed for qPCR (Bio-Rad CFX Manager, USA) using ChamQ Universal SYBR qPCR Master Mix (Q711-02 ...
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bioRxiv - Microbiology 2024Quote: ... and gyrA (control) gene-specific qPCR F/R primers in a 1X SYBR green master mix (Bio-Rad). Threshold and baseline parameters were kept consistent for experiments performed on different days ...