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Citations for Bio-Rad :
151 - 200 of 314 citations for Human NID1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... Collected sera were subjected to ELISA by capturing with human anti-rituximab idiotype antibody (HCA186, Bio-Rad Laboratories), detected with rat-anti-rituximab-HRP antibody (MCA2260P ...
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bioRxiv - Bioengineering 2020Quote: ... Isolated EVs were stained with PE-labelled anti-human TNFR1 (Bio-Rad, cat #MCA1340PE, clone H398, lot 0407) and APC-labelled anti-mouse gp130 antibodies (R&D Systems ...
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bioRxiv - Immunology 2022Quote: ... We used mouse anti-human HLA-ABC (W6/32) conjugated to Alexa Fluor® 647 (MCA81A647, Bio-Rad), which is known to also react with MHC-I of African Green monkey from which Vero E6 cells are derived(48 ...
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bioRxiv - Immunology 2019Quote: ... fatty acid free BSA Efflux was measured after a 4-hour incubation period with 0µg/ml and 50µg/ml of human HDL (#5685-2004, BioRad). Supernatants were collected and the cells lysed with 1% (w/v ...
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bioRxiv - Immunology 2020Quote: ... The binding was detected by an HRP-conjugated anti-human IgG (Fc) CH2 Domain antibody (Bio-Rad MCA647P) used at a 1:5,000 dilution at RT for 1 h and developed as described above.
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bioRxiv - Neuroscience 2023Quote: ... and Bio-Plex Pro Human Inflammation Panel 1 (37-plex) (Cat. No. 171AL001M) from Bio-Rad (Hercules, CA).
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bioRxiv - Plant Biology 2019Quote: ... 5 µg of plasmid DNA were bound to 3 mg gold micro-carriers (1 µm, Bio-Rad) by adding 50 µL of 2.5 M CaCl2 and 20 µL of 0.1 M spermidine ...
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bioRxiv - Bioengineering 2019Quote: ... mobilis competent cells were prepared as previously described (30) and transformed with plasmids by electroporation (Bio-Rad Gene Pulser ...
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bioRxiv - Biochemistry 2022Quote: ... The plasmid DNA was purified by agarose gel electrophoresis using the Model 491 Prep Cell (Bio-Rad). All DNA concentrations are expressed in moles of molecules.
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bioRxiv - Microbiology 2020Quote: ... pBGC plasmid was introduced into our isolate collection by electroporation (Gene Pulser Xcell Electroporator, BioRad, CA, USA). Of note ...
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bioRxiv - Synthetic Biology 2020Quote: ... Transformation of bacterial cells with plasmid DNA was performed by electroporation using a MicroPulser Electroporator (Bio-Rad). GeneJET gel extraction kit and plasmid miniprep kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... or 500 ng to 3 µg for integrative plasmids (MicroPulserTM electroporator Biorad in 2 mm cuvettes (Eurogentec) at 25 µF ...
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bioRxiv - Neuroscience 2023Quote: ... mixed plasmid DNA (50 μg total) was coated on 1 μM-diameter gold particles (Bio-Rad, 1652263) in 0.5 mM spermidine ...
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bioRxiv - Cancer Biology 2023Quote: ... were transformed with the ligated plasmids using the Gene Pulser instrument (Bio-Rad Laboratories, Inc., Hercules, CA). The library densities were monitored and maintained at >107 colony-forming units per microlitre with limiting dilution ...
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bioRxiv - Bioengineering 2023Quote: ... Electrocompetent DH5a bacteria were transformed with the library plasmids using the Gene Pulser Electroporation System (Bio-Rad). The diversity of the plasmid library was determined by the number of clones growing from a representative aliquot of the transformed bacteria on LB agar containing 150 mg/ml ampicillin ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmid transfections and siRNA mediated knock downs were done along with cell seeding using Transfectin reagent (BioRad) and Dharmafect reagent (Horizon discovery ...
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bioRxiv - Cell Biology 2022Quote: Cytokine and growth factor secretion was measured with the Bio-Plex Pro Human Cytokine Screening Panel (Bio-Rad #M500KCAF0Y), a 27-plex assay ...
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bioRxiv - Neuroscience 2021Quote: ... The isolated placental CD34+ single cell suspension was incubated with human CD34- phycoerythrin (PE) (Bio-Rad; dilution 1:25), human CD45-FITC (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... Mono-Mac-6 effector cells previously stimulated with recombinant human IFN-ɣ (Bio-Rad, Hercules, CA, USA; cat. PHP050) at 0.2 µg ml-1 for 3h at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cytokine levels were measured using the magnetic bead-based Bio-Plex Pro Human cytokine 27-Plex (Bio-Rad) according to manufacturer’s instructions and measured the cytokines using the Bio-Plex 200 instrument ...
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bioRxiv - Microbiology 2023Quote: ... The samples were analyzed in duplicates using the Bio-Plex Pro™ Human Cytokine panel (Bio-Rad, Hercules, CA), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Cytokines were quantified in media isolated from MN cultures using the Bio-Plex Pro Human Cytokine kit (Biorad, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: HEK293T cells cultured in 12 cm dishes were transfected with 5 μg plasmid using Transfectin lipid reagent (Biorad) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... the crystal jelly Aequorea victoria gfp was amplified from the pGlo™ Plasmid (Bio-Rad, Hercules, CA, USA) using gfpNF2 and gfpNR at 94°C for 1 min ...
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bioRxiv - Microbiology 2021Quote: ... Demethylated recombined plasmids were then transformed into ZM401 or ZM4 through electroporation using Gene Pulser (Bio-Rad, USA) with 0.1 cm gap cuvettes operated at 18 kV/cm ...
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bioRxiv - Molecular Biology 2020Quote: ... All plasmids were introduced into recipient strains using a standard electroporation protocol according to the manufacturer’s instructions (BioRad Gene Pulser Xcell ...
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bioRxiv - Biochemistry 2021Quote: ... Guide RNA plasmids were transformed into electrocompetent cells containing the Cas protein using a MicroPulser Electroporator (Bio-Rad). Cells were recovered for 100 min at 37°C in LB broth ...
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bioRxiv - Microbiology 2020Quote: ... 100μl of electrocompetent cells was mixed with 10μl (500ng-1μg) of plasmid DNA and transferred to 2mm ice-cold electroporation cuvettes (Gene Pulser Cuvette (BioRad) and tapped gently to avoid air bubbles ...
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bioRxiv - Immunology 2022Quote: ... 25 µg (1 µg/µL concentration) of plasmid DNA was added to electroporation 4 mm cuvettes (Bio-Rad) first and then cells added to the cuvettes ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting plasmids were introduced into each WT cell using particle bombardment (PDS-1000/He, BioRad, Tokyo, Japan), and transformants expressing GFP were screened by fluorescence microscopy (Tachibana et al. ...
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bioRxiv - Microbiology 2024Quote: ... The cell/plasmid mixture was transferred to a chilled Gene Pulser 0.1 cm Cuvette (Bio-Rad 165-2089) and electroporated in a Gene Pulser Xcell (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... and transformed with 300-500 ng of methylated plasmid DNA using a Gene Pulser Xcell (Bio-Rad, Germany) at 2000 V ...
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bioRxiv - Biophysics 2022Quote: ... from Sigma-Aldrich (St. Louis, MO, US). Goat anti-human IgA (IgG, Catalog No. STAR141) was purchased from BIO-RAD (Hercules, CA, US). Human colostrum IgA (Catalog No ...
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bioRxiv - Immunology 2022Quote: ... Primary antibody incubation was performed by incubating slides with diluted monoclonal mouse α-human pan-cytokeratin antibody (Bio-Rad MCA1907T) overnight at 4 °C followed by secondary antibody incubation with HRP-labeled α-mouse antibody for 30 minutes RT.
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bioRxiv - Immunology 2021Quote: Multiplexed cytokine assays were performed according to the manufacturer instructions by using a Bio-Plex Pro Human cytokine screening panel (Biorad) containing 48 human cytokines ...
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bioRxiv - Cancer Biology 2021Quote: ... 48 cytokines were quantified in the cell supernatant using a Bio-Plex Pro Human Cytokine Screening Panel (Bio-Rad, #12007283) according to manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: 17 cytokines secreted from the HCCoC were analyzed using Bio-Plex Pro Human Cytokine Group-I Panel (M5000031YV, BioRad, CA). HCCoCs were exposed to physiological WSS for 6 hours after completion of the preconditioning protocol ...
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bioRxiv - Microbiology 2020Quote: The assay was validated through a combined effort of six veterinary or human diagnostic laboratories by using the following qPCR platform and chemistry combinations: i) CFX 96 (BioRad) and LC480 I (Roche)-QuantiNova Probe RT-PCR Kit (Qiagen) ...
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bioRxiv - Immunology 2021Quote: ... cell-free supernatant was collected and analyzed by Bio-Plex ProTM Human Cytokine Th1/Th2 Assay according to the manufacturer’s instructions (Bio-Rad). All tests were performed in duplicates ...
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bioRxiv - Immunology 2021Quote: Concentration of 27 inflammation-related human proteins were measured in cell culture supernatants by using Human 27-plex BIORAD cytokine panel (Cat. No.171-A1112, BIORAD). Standard curves were generated for all 27 cytokine standards with eight 2-fold serial dilutions starting from 32 ng/ml ...
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bioRxiv - Microbiology 2021Quote: ... Binding of the anti-NTD antibody was detected using a goat anti-human kappa light chain conjugated to horseradish peroxidase (HPR) (BioRad). To normalize for the amount of the bound probes ...
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bioRxiv - Immunology 2023Quote: ... Blocked membranes were incubated with HuCAL anti-bovine Mincle CRD antibodies (2.5 µg mL-1) before incubation with secondary goat-anti-human IgG F(ab’)2: HRP (STAR126P, Bio-Rad) antibody ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with primary antibodies (mouse anti-human Aβ 82E1 [1:500, IBL 27725], rat anti-CD68 [1:250, BioRad MCA1957] ...
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bioRxiv - Cancer Biology 2023Quote: ... The appropriate house-keeping genes to use for data normalization were determined employing the Human Reference Gene Panel (Bio-Rad). Data was normalized with TBP and HPRT1 analysis.
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bioRxiv - Evolutionary Biology 2021Quote: ... Young leaves of aseptically grown tobacco plants were bombarded with plasmid-DNA-coated 0.6 μm gold particles (Bio-Rad) and selected on RMOP media with 500 μg ml-1 spectinomycin (Svab and Maliga 1993) ...
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bioRxiv - Neuroscience 2022Quote: ... At 8DIV cultures were shot with 1.6 micron Gold micro-carriers coated with 30 μg of hSyn.iGABASnFR.F102G plasmid using the Helios gene-gun system (Bio-Rad). Following transfection cultures remained for 5-10 days before experiments were carried out.
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bioRxiv - Synthetic Biology 2022Quote: ... 50 μL of Bt resuspension was electroporated in a 0.1 cm cuvette at RT with 0.5 μL of pDonor plasmid (average ~200 ng) at 2.2 kV using a MicroPulser (Bio-Rad). The cells were recovered in 1 mL of SOB at 37 °C for 2 hrs ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 μg of in vitro-transcribed RNA (2.5 μg of SNX27/LRFN2 RNA and 2.5 μg of the defective helper plasmid) was electroporated into 0.6×107 BHK-21 cells using a Gene Pulser II electroporation system (BioRad) in a gene pulser cuvette (0.2 cm gap) ...
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bioRxiv - Genetics 2020Quote: ... Plasmids were transfected into PEFs by electrical transfection in the condition of 170V/15ms (BIO-RAD, Gene Pulser Xcell). Selection culture medium supplemented with 400 μg/ml G418 for random integration cells and 400 μg/ml G418 ...
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bioRxiv - Immunology 2021Quote: ... Cells and plasmid DNA in solution were transferred into 0.2 cm generic electroporation cuvettes (Biorad Gene Pulser; Bio-Rad Laboratories ...