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Citations for Bio-Rad :
251 - 300 of 314 citations for Human KRT12 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: 10 μg of protein samples harvested from xenografted human cells were separated in pre-casted 4-15% gradient SDS gels (Biorad, #456-1084) and transferred to nitrocellulose membranes (Millipore #IPVH00010) ...
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bioRxiv - Cell Biology 2020Quote: ... TNF-β were detected by using the premixed 48-plex Bio-Plex Pro Human Cytokine Assay (Bio-Rad, Hercules, CA, USA; 12007283) according to the manufacturer’s instructions.
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bioRxiv - Genomics 2020Quote: Human cytokines in the serum were measured by Bio-plex Pro TM Human Cytokine Screening 48 plex Bio-PlexTM 200 System (# 12007283, Bio-Rad, US) and Bio-PlexTM 200 System (Bio-Rad) ...
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bioRxiv - Immunology 2020Quote: ... Multiplexed cytokine measurements on macrophage cell culture supernatants were performed using a custom Bio-Plex Express Human Cytokine kit (Bio-Rad 17004073) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: Autaptic murine striatal neurons (DIV 15-18) were incubated with 1 μg/ml human GABAAR mAb or control antibody (anti-CD52, Bio-Rad, #HCA175) at 37°C for 24 hours ...
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bioRxiv - Cancer Biology 2023Quote: Human and murine breast cancer cells were washed with PBS and lysed on ice using 1x Laemmli Sample Buffer (Biorad #161-0737) supplemented with protease (Sigma-Aldrich #P8340 ...
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bioRxiv - Bioengineering 2022Quote: ... Levels of inflammatory cytokines were quantified using Bio-Plex Pro Human Inflammation Panel 1 37-plex (#171AL001M) magnetic bead-based assay (Bio-Rad Laboratories) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Serum binding antibody assays were performed to evaluate IgG responses to SARS-CoV-2 nucleocapsid and ancestral spike S2 domains and RBD using the Bio-Plex Pro Human SARS-CoV-2 IgG (N, S2, RBD) 4-Plex Panel serology assay (Bio-Rad 12014634) as previously described (8).
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bioRxiv - Immunology 2023Quote: Monitoring of cytokine and chemokine levels was performed using Bio-Plex Pro Human Chemokine 40-plex Panel (Bio-Rad Laboratories, Hercules, California) using a Luminex 200 System ...
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bioRxiv - Immunology 2023Quote: Serum SARS-CoV-2-specific binding antibody assays were performed using the Bio-Plex Pro Human SARS-CoV-2 IgG 4-Plex Panel serology assay (Bio-Rad 12014634) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Pro-inflammatory and pro-fibrotic mediators were measured in 50µl of cell-free tissue culture supernatants using a 37-Plex Bio-Plex Pro™ Human Inflammation Panel 1 (Bio-Rad #171AL001M) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... They were immediately transformed by bombardment with plasmid-coated 1-μm gold particles with a helium-driven particle accelerator (PDS-1000/He; Bio-Rad) using 1350 psi rupture disks and a vacuum of 28 inches of mercury ...
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bioRxiv - Molecular Biology 2021Quote: Samples in 1x Laemmli loading buffer (generally equivalent to 1 µL replication reaction or chromatin recovered from 8 µL plasmid pull-down) were resolved on 10% or 4-15% acrylamide Mini-PROTEAN or Criterion TGX precast gels (Bio-Rad) and transferred to PVDF membranes (Perkin Elmer) ...
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bioRxiv - Molecular Biology 2020Quote: ... 5.0 millions of stably transduced cells were electroporated with 50 μg plasmids by the Gene Pulser X cellTM Electroporation System (Bio-Rad) at 250 V and 1500 microfarads ...
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bioRxiv - Molecular Biology 2019Quote: ... 5-10 pg of pUC18 plasmid were electroporated at 18,000V/cm at a time constant of 7.5 msec using Gene Pulser Xcell (Bio-Rad, Hercules, CA) into 10 μl competent cells ...
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bioRxiv - Evolutionary Biology 2020Quote: ... coli cells were prepared using 10% glycerol (Applichem) and transformed with the corresponding plasmids using a “MicroPulser” electroporator (BioRad, Hercules, US) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... About 0.5 μg of plasmid DNA were mixed with the cell suspension and transferred to ice-cold 1mm gap cuvettes (Bio-Rad). Cells were electroporated in a Bio-Rad Gene Pulser Xcell system using settings ...
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bioRxiv - Developmental Biology 2021Quote: ... or with ACVR1 ECD plasmid DNA that was precipated onto gold beads and administered using a HELIOS Gene Gun (Bio-Rad).
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bioRxiv - Cell Biology 2022Quote: ... 5% sorbitol synchronized ring stage Pf NF54 parasites at 5-8% parasitemia were electroporated with 100 µg of pFC-LINUP plasmid at 0.31 kV and 950 µF using a BioRad Gene Pulser (BioRad, Hercules, CA). Positive selection with 8 nM WR99210 (WR ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in 2 ml of 10% sorbitol and 40 μl per transformation mixed with up to 5 μl of plasmids were electroporated in 2 mm cuvettes (Gene Pulser Xcell BIO-RAD). For autoactivation controls ...
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bioRxiv - Cell Biology 2023Quote: ... 2 × 106 of MEL cells were suspended in 100ul of High-Performance Electroporation Solution (BTXpress, 45-0801) with 5-10 µg of plasmid DNA and electroporated with the Gene Pulser Xcell System (Bio-Rad) using 2 pulses at 200 V for 5 ms ...
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bioRxiv - Microbiology 2024Quote: ... 80µl of ice-cold bacterial cells were mixed with 1µl plasmid (pBAD28 == pEMPTY or pHND10 == pAPY, 100-200ng, key resources table) in chilled 0.1cm cuvettes (Bio-Rad, cat# 1652089) and immediately electroporated (Voltage = 1.8kV ...
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bioRxiv - Molecular Biology 2024Quote: Samples in 1X Laemmli loading buffer (generally representing 1-2 µL of replication reaction or 9 µL of a plasmid pulldown sample) were resolved on 10% or 4-15% acrylamide Mini-PROTEAN or Criterion TGX precast gels (Bio-Rad) and transferred to polyvinyl difluoride (PVDF ...
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bioRxiv - Biochemistry 2024Quote: ... were split into 24-well plates at 90.000c/ well and after 24h the indicated plasmids were transfected using TransFectin Lipid reagent (Bio-Rad 1703352) at a total of 50-66ng/well ...
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bioRxiv - Neuroscience 2019Quote: ... The samples were then analysed in duplicate by means of Invitrogen’s Inflammation 20-Plex Human ProcartaPlex™ Panel (#EPX200-12185-901) and read on the Bio-Plex® 200 system (Bio-Rad, 2016). The data is expressed in pg⋅mL−1 ...
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bioRxiv - Synthetic Biology 2019Quote: ... Roughly 5 ng of genomic DNA was mixed with primers and probes for a human RPP30 copy number assay labeled with HEX (Bio-Rad, Hercules, CA) as an internal control ...
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bioRxiv - Genetics 2020Quote: ... and was quantified by using a QuantifilerR Human DNA Quantification Kit (Applied Biosystem, USA) on a Bio-Rad Real-time PCR System (Bio-Rad Laboratories, USA). The quantification process was performed per the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: ... Cell viability dye Viva Fix 498/521 (#1351115) and mouse anti-human CD138 Alexa Fluor 647 (#MCA2459A647) were obtained from Bio-Rad (Hercules, California). CD138 magnetic beads were procured from Miltenyi Biotec (Auburn ...
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bioRxiv - Cancer Biology 2023Quote: PDEC cytokine secretion was analyzed from cleared PDEC culture supernatants using Bio-Plex Pro Human Cytokine 27-plex assay kit (Bio-Rad, cat. M500KCAF0Y) and Bio-Plex 200 System (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... Cytokine levels in cell culture supernatants was determined using the Magnetic Luminex Performance Assay (Human Base Kit A; R&D Systems, coupled with the Bio-Plex 200 (Bio-Rad). The trimmed median value was used to derive the standard curve and calculate sample concentrations.
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bioRxiv - Neuroscience 2023Quote: The reference gene used in combination with the designed human PMP22 assay was the commercially established AP3B1 (dHsaCP1000001; #10031245, Bio-Rad, Basel, Switzerland) ddPCR copy number variation assay.
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bioRxiv - Immunology 2023Quote: IgE receptor cross-linking (IgECL) on HSMCs was accomplished through sensitization with 1 μg/mL chimeric human IgE anti-NP antibody (MCA333S, BIO-RAD, Hercules, CA) for 24 hours ...
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bioRxiv - Molecular Biology 2019Quote: ... BAC plasmid DNA was extracted by alkali.13 Native and digested BAC DNA was separated using CHEF mapper (Bio-Rad, Hercules, CA).
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bioRxiv - Microbiology 2021Quote: ... 100 ng plasmid DNA and 100 μL of electrocompetent mycobacteria were mixed and transferred to a 2 mm electroporation cuvette (Bio-Rad #1652082). Where necessary ...
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bioRxiv - Cell Biology 2020Quote: The number of integrated plasmid copies in the different lines were quantified using iTaq Universal SYBR Green Supermix (Bio-rad #172-5121) in an iQ cycler and iQ5 Multi-color detection system (Bio-Rad) ...
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bioRxiv - Microbiology 2019Quote: ... The mixture was loaded in a chilled 0.1-cm cuvette with plasmid DNA and subjected to two square pulses at 5 s intervals using the Gene Pulser Xcell (Bio-Rad Laboratories) set at 0.55 kV voltage ...
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bioRxiv - Microbiology 2022Quote: ... 100 ng plasmid DNA and 100 μL of electrocompetent mycobacteria were mixed and transferred to a 2 mm electroporation cuvette (Bio-Rad #1652082). Where necessary ...
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bioRxiv - Biochemistry 2022Quote: ... The plasmids was transformed into NAB69 rho0 strain by high-velocity microprojectile bombardment (Biolistic® PDS-1000/He Particle Delivery System, BIO-RAD) (Bonnefoy & Fox ...
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bioRxiv - Molecular Biology 2023Quote: ... 200 μL aliquots were mixed with 1 μg of the plasmid and transferred to 0.2 cm electro cuvettes (BIO-RAD, Hercules, USA), chilled for 15 min on ice ...
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bioRxiv - Immunology 2020Quote: Staining of 2×105 cells per well was performed with mouse anti-human CD79α (clone HM57, Bio-Rad AbD Serotec, Puchheim, Germany, 1:100) for identification of B cells and Alexa Fluor 647-conjugated rat anti-human CD3∊ (clone CD3-12 ...
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bioRxiv - Microbiology 2022Quote: Cytokine/chemokine concentration was assessed in cell culture supernatants 48 h post transfection by using immunoassays formatted on magnetic beads (Bio-Plex Pro Human Cytokine 27-plex Assay #M500KCAF0Y) (Bio-Rad, Hercules, CA, USA), according to the manufacturer’s protocol via Luminex 100 technology (Luminex ...
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bioRxiv - Biochemistry 2024Quote: ... 3 µL of each of the human plasma samples from the small cohort were diluted in 17 µL of Laemmli sample buffer (Bio-Rad; Hercules, CA, USA) followed by denaturing at 70 °C for 10 min and the resulting denatured 20 µL samples were loaded onto 12% Bis-Tris PAGE gel (GenScript ...
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bioRxiv - Plant Biology 2020Quote: Temperature sensitive mutant cells were transformed with the linearized plasmid by electroporation (500 V, 50 μF, 800□Ω) using GenePulser Xcell (Bio-Rad, CA). The cells were recovered in TAP containing 40□mM sucrose ...
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bioRxiv - Biophysics 2021Quote: ... and transfected with Fluorescence Multiplex-Host Cell Reactivation (FM-HCR) reporter plasmids using the Gene Pulser MXCell Plate Electroporation System (Bio-Rad, #165-2670) using conditions 260 V ...
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bioRxiv - Bioengineering 2021Quote: ... All plasmids in this study have been transformed into cells using electroporation with the Bio-Rad Micropulser™ (Bio-Rad Laboratories, Hercules, CA) and were created using Gibson Assembly[51] using the Gibson Assembly Master Mix (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... and transformed with plasmids by electroporation using Bio-Rad Gene Pulser (0.1-cm gap cuvettes, 1.6 kV, 200 Ω, 25 μF) (Bio-Rad, Hercules, CA, USA) following the method developed for Z ...
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bioRxiv - Microbiology 2024Quote: ... Then 300 ng of plasmids were transformed into 100 μl of competent cells using the bacteria program on the MicroPulser Electroporator (Bio-Rad, CA, USA). After 1 h recovery culture at 37°C in 500 μl of LB ...
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bioRxiv - Microbiology 2024Quote: ... 200 µL of competent cells were mixed with 4 µg of library plasmids and then subject to electroporation (1000V, 50μF, 200Ω) using a Gene Pulser Xcell microbial electroporation system (Bio-Rad, Hercules, CA, USA). With this setting ...
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bioRxiv - Microbiology 2020Quote: ... Levels of the following 27 cytokines were analyzed using a BioPlex Pro™ Human Cytokine 27-plex Assay kit (#M500KCAF0Y, Bio-Rad, Hercules, CA, USA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... utilizing the bead-based immunoassay approach Bio-Plex Pro™ Human Cytokine 40-plex Assay (Cat# 171AK99MR2) on a Bio-plex 200 system (Cat# 171000201) (Bio-Rad Laboratories, Hercules, CA) and the Human Cytokine/Chemokine Magnetic Bead Panel (Cat# HCYTMAG-60K-PX30 ...