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Citations for Bio-Rad :
51 - 100 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade C 16055 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... in nuclear and cytoplasmic reagents from the kit NE-PER as described by the manufacturer (Thermo Scientific).The cytoplasmic and nuclear supernatants were stored at −80°C until used and lysate protein concentration was determined using the DCTM Protein assay (Biorad).
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bioRxiv - Neuroscience 2022Quote: ... The supernatants were stored at −80°C until used and lysate protein concentration was determined using the DCTM Protein assay (Biorad).
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bioRxiv - Neuroscience 2022Quote: ... The supernatants were stored at −80°C until used and lysate protein concentration was determined using the DCTM Protein assay (Biorad).
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bioRxiv - Neuroscience 2022Quote: ... The supernatants were stored at −80°C until used and lysate protein concentration was determined using the DCTM Protein assay (Biorad).
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bioRxiv - Neuroscience 2022Quote: ... The supernatants were stored at −80°C until used and lysate protein concentration was determined using the DCTM Protein assay (Biorad).
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bioRxiv - Neuroscience 2022Quote: ... The supernatants were stored at −80°C until used and lysate protein concentration was determined using the DCTM Protein assay (Biorad).
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bioRxiv - Biochemistry 2023Quote: ... and flash-frozen in liquid nitrogen for storage at −80°C The protein concentration was determined using the DC protein assay (BioRad) per the manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: ... All patients were HIV tested using point-of care Genie™ HIV1&2 test kits (BioRad, USA).
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bioRxiv - Cell Biology 2020Quote: ... lysates were cleared by centrifugation at 16000xg for 5 min at 4°C and the total protein concentration in the lysates was measured by Bradford protein assay (Bio-Rad). For western blot analysis 25 µg of each lysate was boiled for 5 min at 98°C (for the detection of LC3B and ATG9A samples were heated at 60°C for 10 min ...
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bioRxiv - Pathology 2022Quote: ... 2-5%β-mercapto-ethanol for 10min at 70°C and then migrated with protein ladder (Precision plus protein all blue standards, Bio-Rad) in Bio-Rad gels (Criterion TGX ...
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bioRxiv - Cell Biology 2019Quote: ... After centrifugation at 14000 rpm for 8min at 4° C the supernatant was collected and protein concentration determined by the Bradford protein assay (Bio-Rad). The proteins were run on 7%-15% SDS-PAGE gels (50μg per lane ...
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bioRxiv - Immunology 2021Quote: Lysates were centrifuged for 15 minutes at 4°C to recover supernatant and quantified for protein concentration using Protein Assay Dye Reagent Concentrate (Bio-Rad). 40ug of protein was loaded per well for polyacrylamide gel electrophoresis using Mini-PROTEAN Precast Polyacrylamide Gels (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... After centrifugation at 14000 rpm for 20 min at 4°C the supernatant was collected and protein concentration determined by the Bradford protein assay (Bio-Rad). Fifty micrograms of total extract were then loaded in SDS-polyacrylamide gels and transferred onto nitrocellulose membranes for western blot analysis ...
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bioRxiv - Molecular Biology 2022Quote: ... 1× AP3B1-HEX-labelled human reference assay (dHsaCP1000001, BioRAD), 1/40 HindIII (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... 1× AP3B1-HEX labelled human reference assay (Bio-Rad), 1/40 HaeIII (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... and human proinflammatory cytokines were quantified using the Bio-Plex Pro™ Human Inflammation Panel 1 (BioRad). Fluorescence was read using a MAGPIX System (Luminex ...
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bioRxiv - Immunology 2021Quote: ... HIV DNA copy number was evaluated using the QX-100 Droplet Digital PCR system (Bio-Rad, Hercules, CA). The PCR-positive and PCR-negative droplets for HIV gag and RPP30 were determined and template concentrations were calculated by Poisson analysis ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged at full speed for 10 minutes at 4⁰C and the total protein concentration in the supernatant was quantified using the detergent compatible (DC) protein assay (BioRad, 500-0114). Equal amounts of protein were mixed with anionic detergent (4x LDS loading buffer ...
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bioRxiv - Neuroscience 2023Quote: ... The cell suspension was collected and centrifuged at 4°C for 15 minutes at 10,000g and cell protein extracts were stored at −80°C or brought forward for total protein quantification using Bradford assay (Bio-Rad; USA). Cell protein samples were suspended in 2x Laemmli buffer (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... The denatured lysate was then clarified by centrifugation (13200 rpm, 10 min at 4°C) and protein concentration was determined using the DC™ Protein Assay (Bio-Rad). Either equal amounts or a serial dilution of the lysates from various strains were resolved using SDS-PAGE and transferred onto a polyvinylidene difluoride membrane ...
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bioRxiv - Microbiology 2020Quote: ... was added to each well and samples were heated from 25 to 95 °C in 1 °C steps of 1 min each in a CFX96 Touch™ Real-Time PCR Detection System (BioRad). Excitation/emission filters of 492 and 516 nm were used to monitor the fluorescence increase resulting from binding of the Sypro Orange to exposed hydrophobic regions of the unfolding MsSepFcore ...
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bioRxiv - Microbiology 2023Quote: ... was added to each well and the mixture was heated from 25 to 95°C in 1°C steps of 1 min each in a CFX96 Touch™ Real-Time PCR Detection System (BioRad). Excitation and emission filters of 492 and 516 nm respectively ...
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bioRxiv - Microbiology 2022Quote: The concentrations of the 37°C samples were determined with a DC Protein Assay (BioRad). A volume corresponding to 50 μg of the 37°C sample was used for further analysis ...
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bioRxiv - Cancer Biology 2022Quote: ... Lysate was clarified by centrifugation at 4 °C and protein quantified using bradford (Bio-Rad) assay ...
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bioRxiv - Molecular Biology 2020Quote: ... goat anti-human IgG Fc (Biorad; 5211-8004; 1:2000) and rabbit anti-Vinculin (EPR8185 ...
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bioRxiv - Cancer Biology 2021Quote: ... Bio-Plex Pro Human Cytokine ICAM-1 (BioRad, USA; 171B6009M) and Bio-Plex Pro Human Cytokine VCAM-1 (BioRad ...
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bioRxiv - Neuroscience 2020Quote: ... 15 min at +4°C) and the supernatant was used to measure the protein concentrations using the Lowry method (Biorad DC protein assay, Bio-Rad, Richmond, CA) (Lowry et al ...
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bioRxiv - Neuroscience 2023Quote: ... Protein was denatured with 1:1 Laemlli loading buffer (BIORAD) by boiling for 10 minutes on a dry bath (Thermolyne Dri-Bath ...
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bioRxiv - Microbiology 2021Quote: ... and anti-canine distemper virus nucleoprotein mouse monoclonal primary antibody (Biorad) in selected fox tissues.
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bioRxiv - Neuroscience 2024Quote: ... The final virus samples were titered by digital droplet PCR (BioRad) using a REP-specific primer/probe set for libraries and a CMV primer/probe set for recombinant virus (Supplementary Table 2) ...
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bioRxiv - Cell Biology 2019Quote: ... 1:500 Rat anti-C-Myc (BioRad, Hercules, CA), Mouse anti-COMMD1 (Novus Biologicals ...
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bioRxiv - Immunology 2019Quote: ... Biotinylated rat anti-mouse IgG1/c (1:2000, Biorad) or rat anti-mouse IgG2a (1:1000 ...
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bioRxiv - Physiology 2022Quote: ... rabbit polyclonal anti-Cytochrome C (AHP2302, BioRad, 1:200), and mouse monoclonal anti-β-actin (A5441-.2ML ...
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bioRxiv - Microbiology 2020Quote: ... Cell suspensions were clarified by centrifugation (20 000 x g, 10 minutes, 4°C) and total soluble protein quantified58 using a kit (Bio-Rad Protein Assay Kit) and BSA as a standard.
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bioRxiv - Cell Biology 2020Quote: ... Lysates were clarified for 5 min at 10,000 × g at +4□C and protein concentration was determined using DC Protein Assay (Bio-Rad Laboratories, Hercules, CA, USA). For immunoprecipitation ...
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bioRxiv - Biochemistry 2019Quote: ... The microsomal fraction was aliquoted and stored at −80 °C after protein quantification with the Quick Start Bradford Protein Assay Kit (Bio-Rad Laboratories, Munich, Germany).
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bioRxiv - Neuroscience 2023Quote: ... and denaturated by β-mercaptoethanol at 75°C before loading 50-60 µg protein onto 4-20% Mini-PROTEAN TGX Stain-FreeTM Protein Gels (Bio-Rad, Hercules, CA, USA) and electrophoresed at 120 V for 120 min ...
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bioRxiv - Neuroscience 2023Quote: ... and denaturated by β-mercaptoethanol at 75°C before loading 20-30 µg protein onto 4-20% Mini-PROTEAN TGX Stain-FreeTM Protein Gels (Bio-Rad, Hercules, CA, USA) and electrophoresed at 120 V for 120 min ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were incubated at 60°C for 15 min in 4x Laemmli sample buffer (Bio-Rad). 20–40µg liver lysate protein was separated by SDS-PAGE and transferred to a PVDF membrane ...
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bioRxiv - Immunology 2022Quote: ... 25μg of protein were denatured at 95°C for 5min in Laemmli Sample Buffer (BIORAD #1610747) with DTT 0.1M ...
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bioRxiv - Immunology 2022Quote: ... the proteins were incubated 5 minutes at 95°C in Laemmli sample buffer (Bio-Rad laboratories), with or without addition of β-mercaptoethanol (Sigma-Aldrich Chemie) ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 μg protein samples were heated at 95 °C in 4x Laemmli sample buffer (Bio-Rad) for 5 min Samples were run down a Novex 4–12% Tris-glycine gel (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... at 100 °C for 10 minutes and then loaded onto precast protein gels (Bio-Rad, #4561084). The gels were transferred onto PVDF membranes using iBlot PVDF gel transfer (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... and Bio-Plex Pro Human Cytokine VCAM-1 (BioRad, USA; 171B6022M) were used ...
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bioRxiv - Biophysics 2022Quote: ... Samples (1 mg of protein per well) were loaded onto a protein gel (BioRad, 4-15% Criterion TGX Stain-Free Precast Gel) ...
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bioRxiv - Microbiology 2021Quote: ... or a median 588ng (IQR 488-697ng) (HIV) of genomic DNA was combined with ddPCR Supermix for Probes (no dUTPs, BioRad), primers (final concentration 900nM ...
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bioRxiv - Microbiology 2023Quote: HIV-1/N and M strains were quantified by targeting the long terminal repeat (LTR) region using GENERIC HIV Charge virale kit (Biocentric) performed on CFX96 Deep-Well (Biorad) with the followed program RT 10 min at 50°C and 5 min at 95°C ...
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bioRxiv - Biochemistry 2021Quote: ... Virus was inactivated by adding 50 μl of 2X Laemmli buffer (BioRad) and incubating at 95 °C for 10 minutes.
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bioRxiv - Microbiology 2021Quote: Virus or pseudovirus concentrates were lysed in 4x Laemmli buffer (Bio-rad) with 10% β-mercaptoethanol and run on SDS-PAGE gels ...
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bioRxiv - Microbiology 2022Quote: Virus or pseudovirus concentrates were lysed in 4x Laemmli buffer (Bio-rad) with 10% β-mercaptoethanol and run on SDS-PAGE gels ...