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Citations for Bio-Rad :
351 - 400 of 453 citations for Human CLEC4M L SIGN His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... 1x Dream Taq buffer A and 1 μL sample DNA in a total reaction volume of 10 uL using CFX96 Touch Real-Time PCR Detection System (BIO-RAD). The PCR amplification cycle was as follows ...
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bioRxiv - Cell Biology 2022Quote: Cytokine and growth factor secretion was measured with the Bio-Plex Pro Human Cytokine Screening Panel (Bio-Rad #M500KCAF0Y), a 27-plex assay ...
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bioRxiv - Neuroscience 2021Quote: ... The isolated placental CD34+ single cell suspension was incubated with human CD34- phycoerythrin (PE) (Bio-Rad; dilution 1:25), human CD45-FITC (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... Mono-Mac-6 effector cells previously stimulated with recombinant human IFN-ɣ (Bio-Rad, Hercules, CA, USA; cat. PHP050) at 0.2 µg ml-1 for 3h at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cytokine levels were measured using the magnetic bead-based Bio-Plex Pro Human cytokine 27-Plex (Bio-Rad) according to manufacturer’s instructions and measured the cytokines using the Bio-Plex 200 instrument ...
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bioRxiv - Microbiology 2023Quote: ... The samples were analyzed in duplicates using the Bio-Plex Pro™ Human Cytokine panel (Bio-Rad, Hercules, CA), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Cytokines were quantified in media isolated from MN cultures using the Bio-Plex Pro Human Cytokine kit (Biorad, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... smegmatis cells were transformed with 2 µL of the mycobacterial expression vector pNIT cloned with the gene Rv1630 (RpsA) using electroporation with a gene pluser (Bio-Rad, USA). Electrocompetent M ...
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bioRxiv - Molecular Biology 2019Quote: ... in a cartridge containing 20 µL of the reaction mix and 70 µL of Droplet Generation Oil for Probes (ddPCR™ 96-Well Plates #12001925, Bio-Rad). The entire emulsion volume was transferred from the cartridge to a 96-well PCR plate (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... membranes were washed 3x with TBS-T and incubated with either Goat Anti-Mouse IgG (H+L)-HRP Conjugate (Bio-Rad, #1721011) or Goat Anti-Rabbit IgG (H+L)-HRP Conjugate (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was washed three times for 10 minutes with 1x PBS and incubated with Goat Anti-Mouse IgG (H+L)-HRP Conjugate (BioRad, #172-1011). Chemiluminescence was induced by incubation with SuperSignal West Femto Maximum Sensitivity Substrate (ThermoFisher ...
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bioRxiv - Biophysics 2022Quote: ... TMEM87A in buffer 1 was exchanged into soybean l-α-phosphatidylcholine (Soy PC, MillaporeSigma) with the addition of Biobeads SM2 (Bio-Rad) and an hour incubation at a protein:lipid ratio of 1:10 (corresponding to 0.4 mg purified TMEM87A and 4 mg of Soy PC lipid or 1:50 in buffer (5 mM HEPES pH 7.2 ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was soaked with Chemi-Lumi One L or Chemi-Lumi One Super (Nacalai Tesque) for the signal detection using ChemiDoc XRS+ (Bio-Rad Laboratories).
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bioRxiv - Microbiology 2022Quote: ... Milli-Q water was treated with Chelex 100 resin 100–200 mesh sodium form via batch method (50 g/L, Bio-Rad Laboratories). A concentrated stock of SD media not containing Cu2+ ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein samples were combined with the 4x Laemli sample buffer (900 μl of sample buffer and 100 μl β-Mercaptoethanol), heated (95°C, 5mins) and run on SDS-PAGE (protean TGX pre-casted gradient gel, 4%-20%, Bio-rad) and transferred to the Nitro-Cellulose membrane (Bio-Rad ...
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bioRxiv - Plant Biology 2021Quote: ... 20 μl of eluted protein samples were separated on SDS gel (12%) and transferred to PVDF membrane (Immun-Blot® PVDF, Bio-Rad) through semi-wet blotting protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μL of cDNA (10 ng of starting RNA) was amplified per reaction using the iTaq Universal SYBR Green Supermix (Bio-Rad, #1725124) and the Bio-Rad CFX96 qPCR system.
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bioRxiv - Biochemistry 2023Quote: ... Fraction collection was performed using a Masterflex L/S easy-load II (Cole Parmer, 77200-50) running at approx 1 mL/min and BioFrac™ fraction collector (Bio-Rad) with a fraction time window of 2 min ...
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bioRxiv - Microbiology 2023Quote: ... before destaining with Tris buffered saline Tween-20 (TBST) and incubation for 1 hour with blocking buffer (TBST with 50 g/L blocking agent (Bio-Rad, 1706404)) ...
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bioRxiv - Biochemistry 2023Quote: ... 1 μl primer mix (final concentration of 200 nM per primer) were prepared in Hard-Shell PCR 384-well plates (Bio-Rad, USA). Amplification conditions were as follows ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was incubated with secondary antibodies for 1 h at room temperature: goat anti-rabbit IgG (H+L)-HRP conjugate (170-6510, Biorad, 1:10000) and goat anti-mouse IgG (H+L)-HRP conjugate (170-6516 ...
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bioRxiv - Biophysics 2022Quote: ... from Sigma-Aldrich (St. Louis, MO, US). Goat anti-human IgA (IgG, Catalog No. STAR141) was purchased from BIO-RAD (Hercules, CA, US). Human colostrum IgA (Catalog No ...
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bioRxiv - Immunology 2022Quote: ... Primary antibody incubation was performed by incubating slides with diluted monoclonal mouse α-human pan-cytokeratin antibody (Bio-Rad MCA1907T) overnight at 4 °C followed by secondary antibody incubation with HRP-labeled α-mouse antibody for 30 minutes RT.
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bioRxiv - Immunology 2021Quote: Multiplexed cytokine assays were performed according to the manufacturer instructions by using a Bio-Plex Pro Human cytokine screening panel (Biorad) containing 48 human cytokines ...
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bioRxiv - Cancer Biology 2021Quote: ... 48 cytokines were quantified in the cell supernatant using a Bio-Plex Pro Human Cytokine Screening Panel (Bio-Rad, #12007283) according to manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: 17 cytokines secreted from the HCCoC were analyzed using Bio-Plex Pro Human Cytokine Group-I Panel (M5000031YV, BioRad, CA). HCCoCs were exposed to physiological WSS for 6 hours after completion of the preconditioning protocol ...
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bioRxiv - Microbiology 2020Quote: The assay was validated through a combined effort of six veterinary or human diagnostic laboratories by using the following qPCR platform and chemistry combinations: i) CFX 96 (BioRad) and LC480 I (Roche)-QuantiNova Probe RT-PCR Kit (Qiagen) ...
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bioRxiv - Immunology 2021Quote: ... cell-free supernatant was collected and analyzed by Bio-Plex ProTM Human Cytokine Th1/Th2 Assay according to the manufacturer’s instructions (Bio-Rad). All tests were performed in duplicates ...
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bioRxiv - Immunology 2021Quote: Concentration of 27 inflammation-related human proteins were measured in cell culture supernatants by using Human 27-plex BIORAD cytokine panel (Cat. No.171-A1112, BIORAD). Standard curves were generated for all 27 cytokine standards with eight 2-fold serial dilutions starting from 32 ng/ml ...
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bioRxiv - Microbiology 2021Quote: ... Binding of the anti-NTD antibody was detected using a goat anti-human kappa light chain conjugated to horseradish peroxidase (HPR) (BioRad). To normalize for the amount of the bound probes ...
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bioRxiv - Immunology 2023Quote: ... Blocked membranes were incubated with HuCAL anti-bovine Mincle CRD antibodies (2.5 µg mL-1) before incubation with secondary goat-anti-human IgG F(ab’)2: HRP (STAR126P, Bio-Rad) antibody ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with primary antibodies (mouse anti-human Aβ 82E1 [1:500, IBL 27725], rat anti-CD68 [1:250, BioRad MCA1957] ...
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bioRxiv - Cancer Biology 2023Quote: ... The appropriate house-keeping genes to use for data normalization were determined employing the Human Reference Gene Panel (Bio-Rad). Data was normalized with TBP and HPRT1 analysis.
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bioRxiv - Synthetic Biology 2022Quote: ... Yields were between 8 and 12 mg/L. Proteins were analyzed by SDS-PAGE (Supplementary Fig. 2a) using Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad). The gels were run at 200 V for 30 min.
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bioRxiv - Microbiology 2020Quote: ... were added separately to two 45 μL volumes of amplification buffer in PCR tubes and snap cooled by placement in a C1000 Touch Thermal Cycler (BioRad, Hercules, CA, USA) for 3 min at 95°C ...
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bioRxiv - Physiology 2023Quote: ... 50µL from this mixture was transferred to a new tube containing 950µL of PBS and stored at 5°C for subsequent the cell counting (using a TC20 Automated Cell Counter, Bio-Rad, Solna, Sweden) 10 to 30 min later (when the mitochondrial measurements had started) ...
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bioRxiv - Biophysics 2023Quote: ... The ‘free’ dye was separated from the labeled protein by binding the reaction mixture to a 250 μL cobalt affinity resin column equilibrated with 15 CV CoWB (no TCEP) in a Micro-Bio spin chromatography column (Bio-Rad Laboratories # 7326204), washed 15 CV with CoWB and then eluted with 400 mM imidazole in CoWB ...
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bioRxiv - Neuroscience 2023Quote: ... Two µl of RLN3 peptide were added to the center of a 0.2 μm nitrocellulose membrane (Cat. No.1620146, Bio-Rad, Hercules, CA, USA). Non-specific sites were blocked by incubation with 5% BSA in Tris-buffered saline with 0.1% Tween® 20 detergent (TBS-T ...
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bioRxiv - Cell Biology 2024Quote: ... 4 µl of the diluted cDNA was added to a Master mix containing 4 µl SsoAdvanced Universal SYBR® Green Supermix (Bio-rad #1725270) and 0.5 µl of a 10 µM stock of each Forward and Reverse primer (Table 5 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The culture supernatant was obtained by centrifugation and used with the Bio-Plex Pro Human Cytokine 8-plex Assay (Bio-rad) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... For immunohistochemistry (IHC) experiments following primary antibodies were used: IgG anti-human CD3 antibody (1:200, clone CD3-12, Bio-Rad) and anti-PCNA antibody (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated overnight at 4 °C with the following primary antibodies (1:100, in blocking buffer): mouse anti-human MBP (BMK-13, antihuman MBP (BMK-13, MCA5751 Bio-RAD), rabbit anti-human PD-L1 (E1L3N ...
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bioRxiv - Immunology 2021Quote: Recombinant human GFAP protein were run on 12% SDS-PAGE and transferred onto membrane via Trans-Blot Turbo Transfer System (BIO-RAD). Membrane was incubated in blocking buffer (PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... and growth factors in culture supernatants of control or R1881 treated LNCaP cells was determined using the Bioplex Pro Human Cytokine 17-plex assay system (Bio-Rad) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... as previously described 58 and a custom Bio-Rad 6-plex based on the Human Inflammation Panel 1 (BioRad, Cat# 171AL001M). For both assays ...
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bioRxiv - Microbiology 2019Quote: ... Isotypes of HuMAbs in culture supernatant were determined by western blot using anti–human IgG conjugated with horseradish peroxidase (HRP) (Bio-Rad Laboratories ...
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bioRxiv - Developmental Biology 2022Quote: ... anti-SARS CoV-2 Spike Neutralizing antibody (SAD-S35, ACRO, USA) or anti-Human IgG Kappa (STAR 127, Bio-Rad, USA) antibody ...
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bioRxiv - Biochemistry 2021Quote: The details of the protein purification protocol for human plasma sample are described in the Aurum serum mini kit leaflet and a detailed was followed(BioRad, USA). The sample was prepared for 2-D gel electrophoresis following passive rehydration for 16-24 h ...
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bioRxiv - Microbiology 2022Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second serum panel (n=29 ...
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bioRxiv - Molecular Biology 2019Quote: The SPR studies to determine conformation binding of the human chimeric 1A8 and 7C8 mAbs were carried out with the ProteOn XPR36 protein interaction array system (Bio-Rad) based on SPR technology ...