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Citations for Bio-Rad :
101 - 150 of 1031 citations for Hepatitis C Virus Core Antigen HCcAg since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... followed by 15 s at 95 °C and 60 s at 58 °C for 40 cycles using Biorad CFX384 thermocycler (Biorad). The mRNA levels of genes encoding cytokine expression were normalized relative to the mean levels of the housekeeping gene and compared using the 2−ΔΔCt method as described previously2 ...
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bioRxiv - Systems Biology 2020Quote: ... reverse transcription for 20 mins at 46 °C and inactivation for 1 min at 95 °C) in a thermal cycler (C1000 Touch, Biorad). TaqMan Fast Advanced Master Mix (Applied Biosystems ...
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bioRxiv - Systems Biology 2020Quote: ... 40 cycles of PCR denaturing for 5 secs at 95 °C and annealing/extending for 30 secs at 55 °C) in real-time PCR system (CFX384 Touch, Biorad).
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bioRxiv - Microbiology 2020Quote: ... 73105b and 73106f pseudotyped viruses were incubated at temperatures from 37°C to 50°C for 60 minutes using temperature gradient on PCR thermal cycler (BioRad). Pseudoviruses were then aliquoted in a 96-well culture plate ...
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bioRxiv - Biophysics 2022Quote: ... The protein sample was incubated at 40°C and 80 °C respectively for 1 h in a PCR cycler (BioRad) with a heated lid to prevent evaporation ...
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bioRxiv - Microbiology 2019Quote: ... Quantitative real-time PCR was carried out in triplicates and was performed at a 2-step reaction with 95°C denaturation and 56°C annealing and extension for 35 cycles on a CFX96 Real-Time System (BIORAD). Relative quantification of target genes was determined using Bio-Rad CFX manager 3.1 software ...
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bioRxiv - Neuroscience 2022Quote: ... then denatured for 5 min at 95°C and annealed by gradual cooling down at −0.1 °C/sec using a PCR thermocycler (BioRad T100). Annealed oligos were subsequently inserted into BbsI-digested vector pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene #42230) ...
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bioRxiv - Biophysics 2022Quote: ... The mixture was then heated up to 95°C for 5 minutes and cooled down to 30°C at the rate of 1°C per minute using a thermal cycler (BioRad).
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bioRxiv - Molecular Biology 2022Quote: ... at a ratio of 1:2 in 100 µL of TE by heating to 95°C for 3 min followed by cooling to 25°C at 0.1 °C/min in a T100 thermal cycler (Bio-Rad). In Figures ...
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bioRxiv - Molecular Biology 2022Quote: ... and heating to 95°C for 3 min followed by cooling to 25°C at 0.1 °C/min in a T100 thermal cycler (Bio-Rad). Primer extension assays were performed either as time courses (up to 240 min ...
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bioRxiv - Zoology 2022Quote: ... and folded by heating to 95°C for 2 min and slowly cooling down at 0.1 °C per second using a thermocycler (Bio-Rad). DsRNA concentrations were adjusted to 3 or 14 μg / μl using SpeedVac Concentrator (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... The prepared assay plate was exposed to temperature increases from 25 ⁰C to 95 ⁰C with 1 ⁰C increments for 5 s each using a CFX Connect Real-Time PCR Detection System (BioRad). SYBR fluorescence was detected after every temperature increase ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 40 cycles of 95 °C for 5 sec and 60 °C for 30 sec using the CFX 96 real-time systems (Biorad). The 2−ΔΔCt method was used to analyze the data (Schmittgen and Livak ...
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bioRxiv - Systems Biology 2023Quote: ... 40 cycles of PCR denaturing for 5 secs at 95 °C and annealing/extending for 30 secs at 55 °C) in real-time PCR system (CFX384 Touch, Biorad). The results were normalized to GAPDH from the same well ...
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bioRxiv - Cancer Biology 2022Quote: ... 95°C for 2 min followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min (BioRad). The following primers from Integrated DNA Technologies (IDT ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were heated from 10 °C to 95 °C (10 s at each 0.5 °C step) using a CFX Connect qPCR with CFX Manager software (Bio-Rad). Melting temperatures were calculated with DSFWorld27 (by model 2).
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bioRxiv - Microbiology 2023Quote: ... and 40 cycles of PCR (5 s at 95°C followed by 20 s at 60°C) in a CFX Opus 96 Real-Time PCR System (BioRad). Results were analyzed by the Comparative Ct Method (ΔΔCt Method ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by 15 minutes at 50°C and 5 minutes at 85°C in T100 Thermal Cycler (1861096, Bio-Rad). Real-time qPCR reactions were performed using SsoAdvanced Universal SYBR® Green Supermix (1725274 ...
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bioRxiv - Systems Biology 2023Quote: ... reverse transcription for 20 mins at 46 °C and inactivation for 1 min at 95 °C) in a thermal cycler (C1000 Touch, Biorad). For gene expression assays ...
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bioRxiv - Plant Biology 2024Quote: ... using 45 cycles of 15 sec at 95°C and 30 sec at 60°C in a CFX96 cycler (Biorad). Data were normalized to the transcript encoding PP2A subunit A3 (At1g13320 ...
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bioRxiv - Neuroscience 2024Quote: ... 72°C (30s) and 1 cycle of 72°C for 5 min (C1000 Touch Thermal Cycler, Bio-Rad, Hercules, CA). 2–5 μl of the PCR reactions were subjected to gel electrophoresis using a 3% agarose gel in tris-borate-ethylenediamine tetraacetic acid buffer ...
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bioRxiv - Plant Biology 2024Quote: ... using 45 cycles of 15 s at 95°C and 30 s at 60°C in a CFX96 cycler (Biorad). Primers are listed in Additional file 1.
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bioRxiv - Immunology 2024Quote: ... 72°C for 5 minutes (BioRad, Hercules, CA). Genomic PCR was performed using the following primers ...
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bioRxiv - Cell Biology 2020Quote: ... to pH 8.88 first for 5 min at 95 °C followed by 60 °C for 3 h on a T100 Thermal Cycler (Bio-Rad) with the heated lid set to 105 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... 30 second at 72°C and a final extension of 7 minutes at 72 °C in a thermal cycler (T100TM Thermal Cycler, Bio-Rad). Five μl of PCR product was visualized on a non-denaturing 1% agarose gel with a 100bp ladder ...
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bioRxiv - Developmental Biology 2020Quote: ... 50 seconds at 72°C and a final extension of 5 minutes at 72 °C in a thermal cycler (T100TM Thermal Cycler, Bio-Rad). In order to standardize the expression pattern of NvDsx ...
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bioRxiv - Molecular Biology 2020Quote: ... The deamination reaction was then carried out by incubating in a thermal cycler for four cycles of 5 minutes at 70°C followed by 1 hour at 60°C and then desalted with Micro Bio-spin 6 chromatography columns (Bio-Rad). RNA was desulphonated by adding an equal volume of 1 M Tris (pH 9.0 ...
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bioRxiv - Biophysics 2019Quote: ... Temperature was increased at a rate of 1°C per minute from 4°C to 100°C and fluorescence was measured every minute with a qPCR thermocycler in FRET mode (Bio-Rad). Aggregation temperature was determined by locating the global minimum of the second derivative of the raw data ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were heated at a rate of 0.5 °C per minute from 25 °C to 95 °C and fluorescence signals were recorded with the CFX Connect Real-Time PCR Detection System (Bio-Rad). The melting temperatures were analyzed using the derivative plots of the melting curve.
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bioRxiv - Microbiology 2020Quote: ... was added to each well and samples were heated from 25 to 95 °C in 1 °C steps of 1 min each in a CFX96 Touch™ Real-Time PCR Detection System (BioRad). Excitation/emission filters of 492 and 516 nm were used to monitor the fluorescence increase resulting from binding of the Sypro Orange to exposed hydrophobic regions of the unfolding MsSepFcore ...
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bioRxiv - Plant Biology 2021Quote: ... All qPCR reactions were run for 94°C for 2 min followed by 94°C for 15 s and 60°C for 1 min in a BioRad CFX384 Real Time System (Biorad, USA) qPCR machine ...
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bioRxiv - Microbiology 2022Quote: ... SYPRO orange fluorescence data in relative fluorescence unit (RFU) was collected from 10 °C to 95 °C using CFX Connect Real-Time PCR Detection System (Bio-Rad). The temperature corresponding to the lowest point of the first derivative −d(RFU)/dT was determined to be the Tm.
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bioRxiv - Cancer Biology 2022Quote: ... then 40 cycles of 15 seconds at 95°C and 60 seconds at 60°C using CFX96 Touch Real-Time PCR Detection System (Bio-rad). A standard curve was derived from the serial dilutions of DNA template following concentrations ...
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bioRxiv - Biochemistry 2022Quote: ... SYPRO orange fluorescence data in relative fluorescence unit (RFU) was collected from 10 °C to 95 °C using CFX Connect Real-Time PCR Detection System (Bio-Rad). The temperature corresponding to the lowest point of the first derivative ...
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bioRxiv - Microbiology 2021Quote: ... Binding reactions were incubated for 40 minutes at 37 °C then run on a precast 5% TBE acrylamide gel at 4 °C for 45 minutes at 100 V (Bio-Rad). After transfer to Biodyne B Modified Nylon Membrane (ThermoFisher) ...
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bioRxiv - Bioengineering 2020Quote: ... followed by 40 cycles of 95°C for 5 s and 60°C for 30 s (Bio-Rad CFX manager 3.1). Quantification of mRNA levels of different genes was performed using the 2−ΔΔct method ...
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bioRxiv - Microbiology 2021Quote: ... 60°C for 30 sec and 72°C for 30 sec on a Bio-Rad CFX96 qPCR cycler (Bio-Rad, Germany).
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bioRxiv - Microbiology 2022Quote: ... and 40 cycles of PCR (5 s at 95°C followed by 20 s at 60°C) in a CFX Opus 96 Real-Time PCR System (Bio-Rad). Results were analyzed by the Comparative CT Method (ΔΔCt Method) ...
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bioRxiv - Biochemistry 2022Quote: ... was measured over temperatures ranging from 10 °C to 95 °C using a CFX Connect Real-Time PCR Detection System (Bio-Rad). Melting temperature (Tm ...
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bioRxiv - Neuroscience 2022Quote: ... at either 95°C for 5 minutes or at 70°C for 10 minutes and resolved in a 4-15% Precast Gels (Bio-Rad, 12-well ...
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bioRxiv - Microbiology 2023Quote: ... was added to each well and the mixture was heated from 25 to 95°C in 1°C steps of 1 min each in a CFX96 Touch™ Real-Time PCR Detection System (BioRad). Excitation and emission filters of 492 and 516 nm respectively ...
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bioRxiv - Neuroscience 2023Quote: ... with a melt-curve from 65 to 95 °C (in 0.5 °C increments per 5 s) using CFX384 Touch Real-Time PCR Detection System (Bio-Rad, 1845384). Quantification of mRNA was determined using the comparative cycles to threshold CT (ΔΔCT ...
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bioRxiv - Immunology 2023Quote: ... extension at 72°C for 30sec and final extension at 72°C for 5minutes) were conducted in Thermocycler (Bio-Rad, USA). Positive control samples were taken from Molecular Parasitology Laboratory ...
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bioRxiv - Physiology 2023Quote: ... Lysates were then incubated for 5 min at 37 °C and 5 min at 75 °C in a thermocycler (T100, Bio-Rad), immediately followed by storage at -80 °C until further processing.
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bioRxiv - Cancer Biology 2024Quote: ... 40 cycles of 95 °C for 5 s and 60 °C for 10 s using a CFX Connect Real-Time PCR System (Bio-Rad). The relative expression levels of mRNA were calculated using the 2−ΔΔCt method.
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bioRxiv - Immunology 2020Quote: ... 40 cycles of 15 s 95°C and 1 min 60°C) was carried out using a CFX 96 (BioRad, Hercules, USA) qPCR machine ...
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bioRxiv - Cancer Biology 2019Quote: ... followed by 5 s at 95°C and 30 s at 60°C for 40 cycles using an Biorad CFX384 system (Biorad, Hercules, CA). The mRNA levels of each gene were normalized relative to the mean levels of the two housekeeping genes and compared with the data obtained from unstimulated ...
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bioRxiv - Physiology 2021Quote: ... 95°C for 20 s followed by 40 cycles at 95°C for 3 s and 60°C for 30 s using a real-time thermal cycler (CFX Connect; BioRad, Hercules, CA).
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bioRxiv - Neuroscience 2021Quote: ... was incubated at 65°C for 10 min and at 85°C for 1 min into a T100™ thermocycler (Bio-Rad). The heated lysate was subsequently used in cDNA synthesis with SuperScript™ III Reverse Transcriptase kit in a total volume of 20 μl following manufacturer protocol (Invitrogen™) ...
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bioRxiv - Zoology 2023Quote: ... followed by 40 cycles at 95°C for 3 s and 60°C for 30 s using a real-time thermal cycler (CFX Connect; BioRad, Hercules, CA).