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Citations for Bio-Rad :
551 - 600 of 1031 citations for Hepatitis C Virus Core Antigen HCcAg since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The reaction mixture was incubated for 30 minutes at 30°C and stopped by adding 12 μl of Laemmli sample buffer (Biorad). After HMT reactions ...
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bioRxiv - Microbiology 2021Quote: ... for 10 minutes at 37 °C and analyzed with Bio-Rad S3 Cell Sorter (Bio-Rad Laboratories Inc., California, USA) and ProSort™ Software using green fluorescence as a trigger.
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bioRxiv - Immunology 2021Quote: Steady-state equilibrium binding of hCoV-2IG lots was monitored at 25°C using a ProteOn surface plasmon resonance (BioRad). The purified recombinant SARS-CoV-2 RBD proteins were captured to a Ni-NTA sensor chip with 200 resonance units (RU ...
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bioRxiv - Microbiology 2020Quote: ... coated with PBS alone or with 2 µg/ml of SARS-CoV-2 S protein in PBS overnight at 4°C were blocked for 1 hour with 3% nonfat dry milk (Biorad) in PBS ...
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bioRxiv - Genomics 2021Quote: ... centrifuged at 14,000 rcf for 20 min at 4°C and protein amounts in the supernatant determined using the Bradford assay (BioRad) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was subsequently washed 3 times in TBS+0.05 % Tween-20 over 45 min and incubated with secondary antibodies for 1 h at RT (20 °C) after washing 3 times in TBS+0.05 %Tween-20 and developed by enhanced chemiluminescence (Bio-Rad) with Image Quant™ LAS 4000.
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bioRxiv - Neuroscience 2021Quote: ... and equal protein amounts were boiled at 95°C for 10 minutes prior to being run on a gel (BioRad) at 120V ...
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bioRxiv - Microbiology 2022Quote: ... as counterstain to denote stained areas and incubated at 37°C in a humidified chamber according to the manufacturer’s instructions (Bio-Rad kit 357-2112 ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were heated at 95°C for 5 minutes and loaded on 4-15% TGX gels (Bio-Rad, no. 4568083) in running buffer (25 mM Tris ...
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bioRxiv - Cell Biology 2022Quote: ... Electrophoresis was performed for 21 h at 14°C in 0.9% (wt/vol) Pulse Field Certified Agarose (Bio-Rad Laboratories) in exactly 2.4L of 0.5x Tris-borate/EDTA buffer in a Rotaphor apparatus (Biometra ...
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bioRxiv - Molecular Biology 2022Quote: ... The mixtures were incubated for 20 min at 37°C and loaded onto a Bio-Dot Microfiltration Apparatus (Bio-Rad) loaded with nitrocellulose (0.2 μm Amersham Protran ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates were centrifuged at 14,000g for 15 min at 4°C and supernatant protein concentrations were determined by Bradford assay (Bio-Rad).
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... The solution was centrifuged 16,000g for 10 minutes at 4°C and the supernatant containing the mitochondrial or cell lysate proteins quantified (Bio-Rad DC Protein Assay ...
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bioRxiv - Immunology 2022Quote: ... and read fluorescence for 45 seconds at 60°C using the CFX384 Touch Real-Time PCR Detection System (Bio-Rad). The expression levels for each gene were then calculated per the 2-ΔΔCT equation [25].
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bioRxiv - Genetics 2022Quote: ... Approximately 43µl of the newly formed droplet solution were transferred into a ddPCR 96-well plate and then sealed at 180°C for 5 seconds in the PX1 PCR Plate Sealer (BioRad). PCR was carried out with the following conditions ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR was achieved in 12 cycles and at optimized melting temperature (tm) of 68.2 °C thorough C1000 touch thermal cycler (Bio-rad). For single stranded PCR product (ssPCRP ...
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bioRxiv - Neuroscience 2022Quote: ... Regional lysates were centrifuged at 21,000 x g for 20 minutes at 4 °C and the supernatant was removed and suspended in 4X laemmli buffer (BioRad) with 2-mercaptaethanol (BioRad ...
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bioRxiv - Synthetic Biology 2022Quote: ... All reagents except the double-stranded beacon were first assembled in a total volume of 9 μL and run at 37 °C in a CFX96 real-time PCR detection system (Biorad). Both WT SpCas9 and SpCas9-NG are highly active at this temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... The reaction mixtures were held at 10°C until the PCR tubes were removed from the thermocycler (C1000 Touch, BioRad). After ...
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bioRxiv - Molecular Biology 2022Quote: ... The plates were incubated at 30°C and pictures were taken with the setting “Blots: Colorimetric” using a ChemiDoc (BioRAD).
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bioRxiv - Cell Biology 2022Quote: ... PIK3CA c.1633G>A (p.E545K) and PIK3CA c.1624G>A (p.E542K) point mutations were performed on the QX200 ddPCR system (Bio-Rad Laboratories) by using PrimePCR ddPCR Mutation assays according to manufacturer’s instructions (Bio-Rad Laboratories) ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein lysates in Laemmli buffer were separated by electrophoresis on 12% SDS-PAGE gels and transferred for 2 hours at 4°C on to a PVDF membrane (BioRad). After blocking for an hour in 5% dry milk ...
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bioRxiv - Synthetic Biology 2022Quote: ... PFGE was undertaken by running samples on a 1.0% agarose gel in 0.5 X TBE solution at 14 °C on a Bio-Rad clamped homogeneous electric field apparatus (CHEF-DR III, BioRad). 6 V/cm were used with 15 h switch time of 60 s followed by 15 h 120 s at 120° ...
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bioRxiv - Synthetic Biology 2023Quote: ... were cultured regularly in DMEM supplemented with 10% FBS (and 5% penicillin and streptomycinat 37°C with 5% CO2 34,35. Transfection was performed by electroporation using the Bio-Rad Gene Pulser Xcell system ...
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bioRxiv - Molecular Biology 2023Quote: ... plates were incubated for 4d at 30°C and photographed before washing using the ChemiDoc XRS+ molecular imager (Bio-Rad) under the blot/chemicoloric setting with no filter ...
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bioRxiv - Synthetic Biology 2023Quote: ... The reaction was kept at 37°C and recorded using a CFX96 Touch Real-Time PCR Detection System (Bio-Rad). To prevent evaporation the plate was sealed with a transparent sticker ...
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bioRxiv - Neuroscience 2022Quote: ... in nuclear and cytoplasmic reagents from the kit NE-PER as described by the manufacturer (Thermo Scientific).The cytoplasmic and nuclear supernatants were stored at −80°C until used and lysate protein concentration was determined using the DCTM Protein assay (Biorad).
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bioRxiv - Neuroscience 2022Quote: ... The supernatants were stored at −80°C until used and lysate protein concentration was determined using the DCTM Protein assay (Biorad).
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bioRxiv - Neuroscience 2022Quote: ... The supernatants were stored at −80°C until used and lysate protein concentration was determined using the DCTM Protein assay (Biorad).
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bioRxiv - Neuroscience 2022Quote: ... The supernatants were stored at −80°C until used and lysate protein concentration was determined using the DCTM Protein assay (Biorad).
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bioRxiv - Neuroscience 2022Quote: ... The supernatants were stored at −80°C until used and lysate protein concentration was determined using the DCTM Protein assay (Biorad).
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bioRxiv - Neuroscience 2022Quote: ... The supernatants were stored at −80°C until used and lysate protein concentration was determined using the DCTM Protein assay (Biorad).
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bioRxiv - Biochemistry 2023Quote: ... and flash-frozen in liquid nitrogen for storage at −80°C The protein concentration was determined using the DC protein assay (BioRad) per the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... Proteins were separated by SDS-PAGE then transferred to nitrocellulose membrane at 4°C (0.45 μm pore size, Bio-Rad). Membranes were blocked overnight at 4° in Intercept tris-buffered saline (TBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were cleared by centrifugation at 14,000 g for 15 min at 4°C and protein concentration was determined using the Bradford assay (Bio-Rad). Sodium dodecyl sulfate (SDS ...
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bioRxiv - Microbiology 2023Quote: ... and 0.2M DTT) for 10 minutes at 95°C before loading in a 10% SDS-PAGE Gel (Bio-Rad 4561034). Sample proteins were separated in 1X SDS Running Buffer (25mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... These mixtures were then heated up to 95 °C for 5 minutes and slowly cooled to room temperature at the rate of 1 °C per minute using a thermal cycler (BioRad).
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were boiled for 8 min at 95 °C in 1X Laemmli buffer (LB) with β-Mercaptoethanol (BioRad, 1610747), resolved on 10% polyacrylamide gels or pre-cast 4-12% Bis-Tris gradient gels (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Binding was performed with gentle mixing for 1 hour at 4°C and loaded onto a Poly Prep Chromatography Column (BioRad). The loaded resin was then washed 3-4 times with 6 ml Wash Buffer (50 mM sodium phosphate ...
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bioRxiv - Molecular Biology 2023Quote: ... Obtained samples were denatured in a 1xLaemmli buffer at 95°C for 10 min and proteins separated using electrophoresis on a 4-15% mini-protean-TGX-precast-gel (BioRad). Proteins were then transferred on nitrocellulose membranes by a semi-dry method using transblot turbo transfer system (BioRad) ...
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bioRxiv - Immunology 2023Quote: ... Protein denaturation before separation was done by treating the cell lysates for 3 min at 95°C in presence of 1x XT Sample Buffer (BioRad) and 1x XT reducing agent (BioRad) ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were stained with primary antibody in blocking buffer at 4°C overnight: anti-NP (1:5000, OBT1555, Bio-Rad), anti-PA ...
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bioRxiv - Neuroscience 2023Quote: ... using pre-treated (95°C for 10 min to reduce adherence) PCR tubes and optical flat caps (Bio-rad, Belgium). The final reaction volume was 20 µL (10 µM of miniMeCP2 or 20 µM of miniMeCP2T158M) ...
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bioRxiv - Biochemistry 2022Quote: ... The PVDF membranes were then incubated with HRP-coupled secondary antibodies (1:10,000, CST) for 2 h at 25°C and visualized by ChemiDoc XRS (Bio-Rad) instrument.
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bioRxiv - Microbiology 2022Quote: Real-time-PCR was performed with Maxima SYBR Green qPCR Master Mix (Applied Biosystem, #4309155) at an annealing temperature of 60°C on a CFX96 touch Real-Time PCR machine (Biorad) using the following primers:
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bioRxiv - Cancer Biology 2023Quote: ... held at 4 °C) was performed in a C1000 Touch Thermal Cycler with 96-Deep Well Reaction Module (Bio-Rad). After RT reaction ...
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bioRxiv - Genetics 2023Quote: ... DNA was resolved by electrophoresis in a 0.7% agarose gel which was dried for two hours at 50°C using 583 gel dryer model from Bio-Rad. Dried gel was hybridized in Church-Gilbert solution (1% BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... 25 μl of the sample were heated at 95°C for 5 min and resuspended in 4x Laemmli sample buffer (BioRad)/50 mM DTT before SDS-PAGE.
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bioRxiv - Biochemistry 2023Quote: ... Blue Native PAGE gel experiments were performed in a cold room (4 °C) using 10% precast gels (BioRad Mini-PROTEAN) and run at 170 V for 55 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... or poly-acrylamide gels and proteins were transferred overnight at 4°C on a nitrocellulose membrane using a liquid transfer system (BioRad). Non-specific sites were blocked with 5% non-fat dry milk in PBS 0.1% Tween 20 ...