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Citations for Bio-Rad :
101 - 150 of 10000+ citations for Heat Shock Factor Protein 1 HSF1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Protein lysates were mixed 1:1 with 2X Laemmli Sample Buffer (Bio-Rad, Hercules, CA) (5% β-mercaptoethanol ...
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bioRxiv - Microbiology 2020Quote: ... while the HN protein was detected by a mouse monoclonal antibody 8H2 (MCA2822, Bio-Rad). The membranes were then washed with PBST on a shaker 3 times (10 min at RT each time ...
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bioRxiv - Neuroscience 2022Quote: ... Solubilized proteins were then subjected to immunoprecipitation using magnetic beads bound to antibodies (Bio-Rad SureBeads Protein A ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were detected using appropriate secondary HRP-conjugated antibodies and enhanced chemiluminesence reagent (Bio-Rad). Band intensities were quantified using ImageJ software.
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bioRxiv - Immunology 2021Quote: ... antibody bound protein A beads were collected with Poly-Prep® Chromatography Columns (BIO-RAD). After 3 times wash with DPBS ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins attached with antibodies were hybridized with Clarity Western ECL Substrate reagent (Bio-Rad) and visualized in a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was immunoprecipitated with anti-CATSPER1 antibody and Protein A magnetic beads (BIO-RAD) with the same method for recombinant protein co-IP ...
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bioRxiv - Developmental Biology 2023Quote: ... The protein concentration das measured of each eluate sample in a 1:20 dilution by Bradford (BioRad protein assay). Per sample ...
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bioRxiv - Immunology 2020Quote: ... heat-sealed and reverse transcription was performed using a c1000 touch thermal cycler (Bio-Rad). After reverse transcription ...
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bioRxiv - Developmental Biology 2024Quote: ... which was heat-sealed with foil and placed in C1000 Touch Thermal Cycler (Bio-Rad). Thermal cycling conditions were as follows ...
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bioRxiv - Physiology 2023Quote: ... The ddPCR plate was sealed with a Pierceable Foil Heat Seal (Bio-Rad Cat #1814040) and the PX1™ PCR Plate Sealer (Bio-Rad Cat #1814000) ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentrations were determined using Bradford Assay Kit 1 (Bio-Rad) and 30µg of protein were loaded onto 8% SDS-Page Gels (Thermo Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... incubated with a secondary antibody (1:3000 HRP–linked Anti-Rabbit antibody Bio-Rad) and protein bands were visualized with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermofisher Scientific #34580 ...
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bioRxiv - Molecular Biology 2021Quote: ... 1:3000 or 1:10000 (for H3) dilutions of secondary antibodies (BioRad) (1 h at RT ...
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bioRxiv - Immunology 2021Quote: ... F4/80 antibody | Cl:A3-1 #MCA497PBT (Bio-Rad); BV650 Hamster Anti-Mouse CD11c Clone HL3 (RUO ...
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bioRxiv - Biochemistry 2021Quote: ... primary antibody (MCA4976, 1:50, BioRad, Hercules, CA) were incubated at 4°C overnight ...
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Stem cell-derived macrophages as a new platform for studying host-pathogen interactions in livestockbioRxiv - Immunology 2021Quote: ... Antibodies used were CD14 (Biorad, #MCA1218F, 1:50) with isotype control (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-CD63 antibody (1:200 dilution, Biorad, MCA4754GA), and anti-Hsp70 antibody (1:500 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... CD68 (MCA1957, Bio-Rad Antibodies; 1:200 dilution), TDP-43 (12892-1-AP ...
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bioRxiv - Biochemistry 2023Quote: ... horseradish peroxidase-conjugated secondary antibodies (1:10000, BioRad) were used followed by detection by chemiluminescence (SuperSignal Pico ...
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bioRxiv - Neuroscience 2024Quote: ... CD68 primary antibody (1:200, Bio-Rad, MCA1957GA) was diluted in blocking buffer and solution was left on the slides overnight at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... and α-mouse secondary antibodies (BioRad; 1:10,000), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... gossypii proteins) or TBS (S. cerevisiae proteins) supplemented with 1 mM DTT using pre-equilibrated BioSpin-6 spin columns (BioRad) or ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal amounts of protein (25 µg) were denatured 1:1 in 2X Laemmli buffer (BIO-RAD) at 95°C for 10 minutes then held on ice ...
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bioRxiv - Pathology 2021Quote: ... Protein samples were first diluted 1:1 with Laemmli sample buffer (Bio-Rad, Hemel Hempstead, UK) containing 5% β-mercaptoethanol (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were detected using secondary HRP-conjugated α-mouse antibody and enhanced chemiluminesence reagent (Bio-Rad).
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: ... 20 mg of antibody were loaded on 50 µl Affi-Prep® Protein A Resin (Biorad) (multiply this with the number of 2nd CHIP’s planned and plan one sample to check the ChIP efficiency of the 1st ChIP step) ...
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bioRxiv - Cancer Biology 2022Quote: ... the lysates were incubated with primary antibody conjugated SureBeads Protein G Magnetic Beads (Bio-Rad Laboratories) for 1 hour rotating at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Dynabeads-bound antibody-proteins (IP samples) were eluted with 1x Laemmli Sample Buffer (1610737EDU, Bio-Rad) for 20 min at 65°C while shaking at 1000 rpm in ThermoMixer F1.5 (Eppendorf) ...
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bioRxiv - Cancer Biology 2023Quote: ... conjugated antibodies was used to detect desired protein by chemoluminescence with ECL (Bio-Rad, #170-5061). For immunoprecipitation ...
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bioRxiv - Cancer Biology 2023Quote: ... fresh 1ng/ml recombinant human fibroblast growth factor (FGF) basic (BioRad, Veenendaal, The Netherlands) and 0.1mM L-ascorbic acid (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... fresh 1ng/ml recombinant human fibroblast growth factor (FGF) basic (BioRad, Veenendaal, The Netherlands) and 0.1mM L-ascorbic acid (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... secondary antibodies were incubated with the PVDF membrane for 1 hour at room temperature (Goat anti-rabbit antibody, BioRad, 1:3000). Then ...
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bioRxiv - Molecular Biology 2019Quote: ... the membrane was incubated overnight at 4°C with primary antibodies diluted 1:1000 followed by a further 1 h incubation in the corresponding horseradish peroxidase conjugated secondary antibody (Bio-Rad) at a 1:10000 dilution ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: ... Labeled EV and cells treated or not with these EV were lysed in PBS containing 1% SDS and protein concentration was determined using the DC™ Protein Assay from Biorad. Samples were then processed and analyzed using nano-LC-MS/MS ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: ... EV were lysed in PBS containing 1% SDS and protein concentration was determined using the DC™ Protein Assay from Biorad. Samples were then processed and analyzed using nano-LC-MS/MS ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: Cells or EV were lysed in PBS containing 1% SDS and protein concentration was determined using the DC™ Protein Assay from Biorad. 2μg of proteins were electrophoresed on SDS-PAGE ...
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bioRxiv - Biophysics 2022Quote: ... Resulting membranes were resuspended in 100 μL solubilization buffer (1xPBS, 1% DDM, EDTA-free protease inhibitors) and total protein concentration was quantified using DC protein assay (Bio-Rad). For each condition ...
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bioRxiv - Neuroscience 2020Quote: ... rat anti-Myelin Basic Protein (BIO-RAD, reference MCA409S, 1/1000 dilution), rabbit anti-β-Tubulin III (Tuj1 ...
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bioRxiv - Biochemistry 2021Quote: Purified proteins concentrated to 1 mL or gel filtration standards (BioRad, 1511901) were injected on to Hi-Load pg200 column and 150 mL eluate was collected as 1.0 mL fractions at 1.0 mL/min. ...
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bioRxiv - Neuroscience 2021Quote: ... All Blue Prestained Protein Standards (1-3 µl; BioRad Laboratories, Hercules, CA) were used to identify band molecular weights ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were prepared in 1× Laemmli buffer (Bio-Rad, Cat. No. 1610747) supplemented with β-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... 100 µl/ well of protein G-HRP conjugate (1:3000, Bio-Rad) in PBS was added and incubated for 1 hr at 37°C and then washed with PBST ...
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bioRxiv - Cell Biology 2021Quote: ... overnight following with 15 μL prewashed protein A/G beads (1:1 mixture, 1614013 and 1614023, Biorad) incubation for another 1.5 hr ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked in 5% dry non-fat milk in PBST and probed using PRP8a antibody (1:500) and an HRP coupled anti-Rabbit secondary antibody (Biorad, 1:10000). All antibodies were diluted in 1% dry nonfat milk in PBST ...
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bioRxiv - Neuroscience 2019Quote: ... Antibodies used were: F4/80 (BioRad MCA497G 1/400), Iba1 (Wako 019-19741 ...
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bioRxiv - Neuroscience 2022Quote: ... rat anti-MBP (1:250, Bio-Rad antibodies MCA409S), rabbit anti-TPPP/p25 (1:1000 ...
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bioRxiv - Plant Biology 2023Quote: ... anti-rabbit secondary antibodies (BIO-RAD, 1:5000 dilution) or Pierce™ HRP-conjugated Streptavidin (Thermo scientific ...
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bioRxiv - Neuroscience 2023Quote: ... rat monoclonal antibody against Cd68 (1:200, BioRad, MCA1957), rabbit polyclonal antibody against Map2 (1:200 ...
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bioRxiv - Molecular Biology 2023Quote: ... and/or fluorophore conjugated secondary antibodies (Biorad, 1:2,000), washed three times and imaged ...