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Citations for Bio-Rad :
51 - 88 of 88 citations for FabFc ZAP Hug M since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Labeling efficiency was determined by measuring the absorbance of pyrene (χ346nm = 42,000 M-1cm-1) and protein concentration by 660 nm assay (Bio-Rad). An SDS-PAGE shift assay for isolating single-mSA bound ...
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bioRxiv - Microbiology 2021Quote: Two micrograms of each RNA sample was loaded on 10% polyacrylamide gels containing 7 M urea or loaded onto 10% Criterion TBE-urea precast gels (Bio-Rad) and electrophoresed at 85 V ...
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bioRxiv - Biochemistry 2021Quote: ... The cell extract was clarified by filtering through 0.4 µm and 0.22 µm filters prior to loading onto ~4 mL of Ni-NTA resin (Profinity™ IMAC resin, Bio-Rad). To allow the His-tag protein to bind the resin it was incubated overnight at 4°C on a rolling shaker ...
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bioRxiv - Microbiology 2021Quote: ... or wet transferred using 0.5 M sodium phosphate transfer buffer (at a constant 1 Amp for an hour using a Bio-Rad apparatus). Membranes were blocked in 5% milk in PBS-T and probed with primary antibody (overnight at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... supplemented with DTT (0.1 M endconcentration) and then subjected to SDS-PAGE on 12% or 4–20% gradient gels (mini-PROTEAN TGX; Bio-Rad). Proteins were transferred to PVDF membranes (TransBlot® TurboTM LF PVDF ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were then transferred to a 0.2 µm nitrocellulose membrane (Amersham) in Tris/glycine/ 20% MeOH at 400 mA for 2h using a tank blot system (Bio-Rad). In order to detect any blotting artifacts the proteins on the membranes were visualized with Ponceau red solution ...
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bioRxiv - Pathology 2021Quote: ... 0.10 M) at 50°C overnight followed by cleanup on 150 μL cation exchange media (AG50WX8, Bio-Rad, Hercules, CA, US) on top of C18 SPE columns (Strata C18-E ...
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bioRxiv - Biochemistry 2019Quote: ... was transferred to a new tube with 50 μL 2 M HCl and boiled for 2 hours at 95°C in a C1000 thermocycler (Bio-Rad) to hydrolyze all glucans to glucose ...
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bioRxiv - Microbiology 2020Quote: ... and washed three times with phosphate buffered saline (PBS) buffer containing 0.1 M EDTA followed by two chelex (BioRad, cat # 1422822)-treated PBS buffer washes to remove all external metal ions ...
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bioRxiv - Synthetic Biology 2021Quote: ... Boiled samples were fractionated on a 37.5:1 Acrylamide:Bis-Acrylamide SDS-Page gel and then transferred to a 0.2 μ m nitrocellulose membrane using a Mini-PROTEAN tank (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... Primers and probes used for IAV-M, IBV-HA were previously reported (World Health Organization, 2017) and synthesized by Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2022Quote: ... The immunoprecipitated proteins from three treatments were mixed and either denatured by 9 M Urea/20 mM HEPES buffer or mixed with 2x Laemmli Sample Buffer (1610737, Bio-Rad) and separated by SDS-PAGE to analyze individual fractions ...
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bioRxiv - Neuroscience 2022Quote: ... The pellet was resuspended in 50 μL 8M urea buffer (8 M urea, 100 mm NaHPO4, pH 8.5) and boiled in Laemmli sample buffer (BioRad, 161-0737) at 98°C for 5 min ...
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bioRxiv - Molecular Biology 2023Quote: ... The fragmentation step was then stopped by adding Tris pH 6.8 (0.4 M final concentration) and then samples were cleaned-up on Micro Bio-Spin P-30 gel columns (Bio-Rad Laboratories). 4sU-RNA was then biotinylated using 0.1 mg/ml MTSEA biotin-XX linker (Biotium ...
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bioRxiv - Neuroscience 2023Quote: ... The pellet was resuspended in 8M urea buffer (8 M urea, 100 mm NaHPO4, pH 8.5) and boiled in Laemmli sample buffer (BioRad, 161-0737) at 98°C for 5 min ...
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bioRxiv - Microbiology 2024Quote: Total RNA was loaded onto 8 to 12% PAGE–7 M urea gels and transferred to Hybond-N+ (GE) membranes using a Trans-Blot Turbo Transfer system (Bio-Rad) in 1X TBE ...
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bioRxiv - Biophysics 2024Quote: ... supernatant was filtered with 0.45 µm filter (Fisher) and loaded to HiTrap Q column (Cytiva) on an NGC Fast Protein Liquid Chromatography (FPLC) station (Bio-Rad). The flow-through fraction was mixed with CM Sepharose FF resin (GE HealthCare Lifesciences ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were resuspended in a small amount of buffer (6 M urea, 50 mM Tris–Cl pH 7.5) and quantified by the BCA quantification method (Bio-Rad, Hercules, CA). Urea concentration was reduced to 4 M and the pH was adjusted to 8.8 with 25 mM Tris–HCl ...
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bioRxiv - Immunology 2021Quote: ... One-step RT-PCR was conducted using matrix (M) segment specific primers (20) and performed on a T100™ Thermal Cycler (Bio-Rad) utilizing the PrimeScript™ One-Step RT-PCR kit (Takara Bio ...
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bioRxiv - Biophysics 2020Quote: ... 50 µl of a 10− 6 M biotinylated and 10−7 M dye-coupled oligonucleotide solution were co-incubated to anneal the oligonucleotides at 80 °C using a Thermocycler (BioRad, C1000 ThermoCycler), followed by sample cooling to 4 °C with -1 °C/min ...
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bioRxiv - Bioengineering 2020Quote: ... Samples were denatured by incubating at 95°C for 10 minutes and loaded onto a denaturing 10% polyacrylamide gel with 8 M Urea in a Mini-PROTEAN® Electrophoresis System (BIO-RAD). Electrophoresis was performed at 240 V for 45 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... Aliquots of 50 μg protein in SDS-PAGE sample buffer containing 0.2 M dithiothreitol were heat-denatured for 5 min at 95 °C then electrophoresed on AnyKD midi criterion™ gels (Bio-Rad) in ProSieve™ EX running buffer (Lonza) ...
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bioRxiv - Immunology 2021Quote: ... the enzyme reaction was stopped with 50 μL of 1 M sulfuric acid per well and the absorbance was measured in Bio-Rad Model 550 microplate reader (Bio-Rad Laboratories). Sera were assayed in duplicates and antibody titer represents the last reciprocal serum dilution above blank.
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bioRxiv - Molecular Biology 2020Quote: ... 4°C and the supernatant incubated during 4 h at 4°C with 50 µl of V5 magnetic beads (20 mg/mL of Dynabeads® M-280 Tosylactivated, Invitrogen, coupled with Anti V5-Tag Antibody, clone SV5-Pk1, Bio-Rad). The unbound fraction was discarded and the beads washed 2 times with 1ml of IP Buffer and additionally twice more with 1 ml ice cold PBS + protease inhibitors ...
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bioRxiv - Molecular Biology 2023Quote: ... and 95 % humidity to let them attach and recover to a concentration of 1×106 cells/m to 20 million cells counted using an automated Cell Counter System (Countess TC20, Biorad, Hercules, CA) which assessed cell viability via trypan blue exclusion ...
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bioRxiv - Plant Biology 2023Quote: ... and proteins were separated in SDS/urea–PAGE (15% polyacrylamide, 6 M urea) (Laemmli, 1970) using mini-protean apparatus (Bio-Rad USA). These separated proteins were transferred to a nitrocellulose membrane using trans-blot apparatus (Bio-Rad USA) ...
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bioRxiv - Microbiology 2023Quote: ... extracellular proteins were separated on SDS 15% (m/v) polyacrylamide gels and electroblotted to Immun-Blot polyvinylidene difluoride membranes (Bio-Rad, USA) using a Mini Trans-Blot electrophoretic transfer cell (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: ... The reactions were stopped with 1 M H2SO4 after 20 min and the absorbance was measured in a microplate reader (Benchmark; Bio-Rad Laboratories) at 490 nm.
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bioRxiv - Neuroscience 2023Quote: ... Samples were prepared for gel electrophoresis by adding a volume containing 20 ug protein to 3 uL 1 M DTT and 7.5 μL 4X Laemmli sample buffer (BioRad Cat. No. 1610747) and topping up to 30 μL with deionized water ...
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bioRxiv - Biochemistry 2024Quote: ... DNA fragments was separated on 20% denaturing acrylamide gel (5% crosslinker, 7 M urea, 1X TBE buffer) using Criterion™ cell apparatus (Bio-Rad) at 300 V for 40 to 60 minutes ...
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bioRxiv - Microbiology 2020Quote: ... The precipitated proteins were then dissolved in 6 M urea/50 mM ammonium bicarbonate buffer (pH 8) for protein quantitation using DC™ protein assay (Bio-Rad Laboratories) and trypsin digestion.
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bioRxiv - Microbiology 2021Quote: ... 12 – 16 individual colonies from each condition were selected from the m-TSAYE plates and DNA was extracted using Bio-Rad InstaGeneTM matrix (Bio-Rad, Hercules, CA) following the manufacturer’s protocol for DNA extraction from bacterial cells ...
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bioRxiv - Cell Biology 2023Quote: ... Total nascent peptides were detected by in-gel imaging at 647 µm and total protein was detected by far red epi imaging after staining with 0.1% Coomassie brilliant blue (BioRad ChemiDoc MP Imaging System). For detection of nascent insulin and tubulin ...
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bioRxiv - Plant Biology 2020Quote: ... The qRT-PCR was performed using 3.5 μl of diluted cDNA and 5.5 μM primers listed in Table 2 (C.-M. Lee and Thomashow, 2012; Wu et al., 2008)using iTaqTM Universal SYBR® Green Supermix (Bio-Rad, cat# 1725121) with the CFX 384 TouchTM Real-Time PCR Detection System (Bio-RAD) ...
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bioRxiv - Biophysics 2020Quote: ... We visualized the transcription products by electrophoresis in a denaturant gel (23% polyacrylamide, 7 M urea) run at 800V for 2 h (Bio-rad, Hercules, CA, USA).
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bioRxiv - Synthetic Biology 2021Quote: ... diluted 1:200 in 10 mM Tris-HCl pH 7.5 and 9 μL loaded (along with 1 μL 1 M DTT and 10 μL 2X Laemmli Buffer) into an Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gel (Bio-rad). For Western blot ...
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bioRxiv - Neuroscience 2019Quote: ... or BIC (20 μM) were added to the culture and cells were harvested with a 1:1 mixture of 2X Laemmeli buffer (BioRad, 1610737, 1:20 beta-mercaptoethanol) and radioimmunoprecipitation assay (RIPA ...
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bioRxiv - Plant Biology 2021Quote: ... Leaf disks were ground to a fine powder evenly with a grinding tool and 120 μl extraction buffer added (30 μl Laemmeli sample buffer 4x [Bio-Rad], 24 μl DTT 1 M, 66 μl dH2O). Tubes were vigorously vortexed for 90 seconds ...