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Citations for Bio-Rad :
151 - 200 of 7407 citations for Endothelin 1 ET 1 ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... cDNA was prepared from 1 µg of RNA using iScript cDNA synthesis kit (Bio-Rad). Quantitative RT-PCR was performed with Lightcycler 480 SYBR Green I Master (Roche ...
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bioRxiv - Neuroscience 2020Quote: ... 1 µg of RNA was reverse transcribed using the iScript cDNA synthesis kit (Bio-Rad). Quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2022Quote: ... RNA (1 ug total) was reverse transcribed using iScript cDNA Synthesis Kit (Bio-Rad, 1708891) and diluted to 100 ul in ddH2O for subsequent qPCR analyses ...
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bioRxiv - Neuroscience 2019Quote: ... 1 µg of RNA was reverse transcribed using the iScript cDNA synthesis kit (Bio-Rad). Quantitative PCR (qPCR ...
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bioRxiv - Biochemistry 2021Quote: ... cDNA synthesis was performed with 1 µg RNA using the iScript cDNA Synthesis kit (www.Biorad.com). cDNA was diluted in water in a 1:10 ratio and 1µl was used for RT-PCR was performed with an iCycler (Biorad ...
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bioRxiv - Cell Biology 2021Quote: ... We used 1 μg RNA to transcribe cDNA with a reverse transcription kit (Bio-Rad). Most of RT-qPCR primers were from the Harvard Primer Bank (https://pga.mgh.harvard.edu/primerbank/) ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of RNA was reverse transcribed using iScript™ Advanced cDNA Synthesis Kit (Biorad). qRT-PCR was then carried out to assay TF (TF ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA (1 μg) was reverse transcribed using the iScript cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was synthesized from 1 μg of total RNA using iScript cDNA Synthesis Kit (BioRad) and BioRad T100 thermocycler ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was synthesized from 1 μg total RNA using the iScript Reverse Transcriptase kit (Biorad). qPCR reactions were carried out 2x IQ SYBR green (Biorad ...
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bioRxiv - Neuroscience 2023Quote: 1 μg of total RNA was reverse transcribed using iScript cDNA Synthesis Kit (Bio-Rad). A volume corresponding to 5 ng of initial RNA was employed for each real-time PCR reaction using PowerUp SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg of total RNA was reverse-transcribed using iSCRIPT cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s protocol ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 1 μg of RNA was used to synthesize complementary DNA using the iScript kit (BioRad). DNA was diluted to 10 ng/μl and 2 μl were used in each RT-qPCR reaction with 10 μl of iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Genetics 2023Quote: ... RNA (1 µg) was reverse transcribed to cDNA using iScript Advanced cDNA synthesis kit (BioRad). Each 96-well hypoxia array (BioRad ...
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bioRxiv - Bioengineering 2023Quote: ... Total RNA (1 μg) was reverse transcribed by an iScript cDNA synthesis kit (Bio-Rad) and qPCR was performed using a TaqMan Mastermix kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcriptions were performed for 1 µg RNA using iScript cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: cDNA was synthesized from 1 µg of the total RNA using iScript Kit (BIO-RAD Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... 500 ng to 1 µg RNA was reverse transcribed using iScript cDNA synthesis kit (Biorad). RT-qPCR was performed using SsoAdvanced Universal SYBR Green Supermix (Biorad) ...
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bioRxiv - Neuroscience 2023Quote: ... 1 ug of RNA was used for cDNA synthesis using iScript cDNA synthesis kit (Biorad), according to the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2023Quote: ... 1 μg total RNA was converted to cDNA using the iScript cDNA synthesis kit (BioRad). RT-qPCR reactions were performed using Fast Sybr Master Mix (ABI ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μg RNA was used for cDNA synthesis using the iScript cDNA Synthesis Kit (BioRad) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized from 1 µg RNA with the iScript cDNA Synthesis Kit (Bio-Rad). Real-time PCR (RT-PCR ...
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bioRxiv - Cell Biology 2024Quote: ... 1 μg of total RNA was reverse transcribed using iScript cDNA Synthesis Kit (Bio-Rad). qPCR used PowerUp SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: ... and the Bio-Plex Pro™ Human Inflammation Panel-1 Kit (37-Plex, Bio-Rad).
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies were anti-mouse (Bio-Rad, #170-6516, dilution 1:1000 or 1:2000) and anti-rabbit (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... 1:5000 and 1:10000 dilutions of the libraries in triplicate on iQ5 (Bio-Rad) and CFX96 Touch qPCR machines ...
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bioRxiv - Plant Biology 2021Quote: ... 0.05% SDS) and a semi dry transfer cell (Bio-Rad, 1 mA/cm2, 1 h). Membrane was blocked with protein free blocking solution T20 (Pierce ...
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bioRxiv - Microbiology 2019Quote: ... was diluted 1:5000 in 1% (w/v) casein blocking buffer (Bio-Rad, 161-0782). Blots were incubated at room temperature for 2 h ...
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bioRxiv - Immunology 2020Quote: ... A total of 50μg protein was diluted 1:1 with 2x Laemmli buffer (Bio-Rad), resolved by SDS-PAGE and transferred into PVDF membranes ...
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bioRxiv - Bioengineering 2021Quote: ... samples were mixed 1:1 with 2x Laemmli sample buffer with 2-mercaptoethanol (Bio-Rad) and boiled for 10min ...
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bioRxiv - Biochemistry 2021Quote: Purified protein was mixed in 1:1 ratio with 2X laemmli loading dye (Bio-rad) prepared according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 1× iTaq Universal SYBR Green Supermix or 1× iTaq Universal Probe Supermix (Bio-Rad, USA), 300 nM each of forward primer (late RT product ...
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bioRxiv - Physiology 2021Quote: ... Sections were incubated with F4/80 antibody (Clone Cl:A3-1, Bio-Rad, MCA497R, 1:100) followed by biotinylated antibody (Vector ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated with the HRP-conjugated secondary antibodies (1:2500-1:10000, Bio-Rad) for 1h at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein lysates were mixed 1:1 with 2X Laemmli Sample Buffer (Bio-Rad, Hercules, CA) (5% β-mercaptoethanol ...
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bioRxiv - Physiology 2023Quote: ... prepared at a 1:1 ratio and acquired using the ChemiDoc MP System (Bio-Rad).
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bioRxiv - Genetics 2022Quote: Samples taken from prior assays were mixed 1:1 with Laemmli Sample Buffer (Bio-Rad) with 10% 2-mercaptoethanol and vortexed to ensure adequate mixing ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was diluted 1:1 with Laemmli Sample Buffer (1610737, BIO-RAD, Hercules, CA) and heated to 80°C for 30 min.
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bioRxiv - Immunology 2024Quote: Samples were prepared in 1 x Laemmli buffer with or without 1 x dithiothreitol (Biorad). Samples were then heated for 10 min at 95 °C and loaded onto a precast NuPAGE™ 4-12 % Bis-Tris polyacrylamide gel in NuPAGE™ MES SDS running buffer (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2019Quote: ... or BIC (20 μM) were added to the culture and cells were harvested with a 1:1 mixture of 2X Laemmeli buffer (BioRad, 1610737, 1:20 beta-mercaptoethanol) and radioimmunoprecipitation assay (RIPA ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated with secondary antibodies for 1 hour at room temperature (anti-rabbit or anti-mouse, 1:10.000, Bio-Rad, anti-goat, 1:7500, Pierce). Antibody binding was detected by chemiluminescence(Clarity kit ...
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bioRxiv - Biophysics 2023Quote: ... Single-stranded cDNA was synthesized from 1 µg of total RNA as template using a commercially available kit (iScript cDNA Synthesis Kit, Biorad). RT-qPCR analysis of nascent mRNA abundance was performed in duplicate using iQ SYBR Green Supermix (Biorad #1708880 ...
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bioRxiv - Molecular Biology 2023Quote: ... One aliquot was used to synthesize single-stranded cDNA starting from 1 µg of total RNA using a commercially available kit (iScript cDNA Synthesis Kit, Biorad).
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bioRxiv - Microbiology 2020Quote: ... 10.5 μl template DNA and 1 μl respective primer pairs (200 nM final concentration, supplementary table 1) were mixed and distributed into a 96-well qPCR plate (Bio-Rad). The plate was incubated 10 min at room temperature to digest the target DNA before droplets were generated in an Automated Droplet Generator (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... 5 µl HeLa lysate was preincubated with 1 µl of accessory protein for 10 min at room temperature in PCR plates (HSP9601, Bio-Rad). Reaction was started by adding 2 µl reaction mix ...
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bioRxiv - Microbiology 2023Quote: ... 550 µl of the assembled community was added into each well of the “Community Plates Day 1” with an epMotion 96 (Bio-Rad) semi-automated electronic 96 channel pipette ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal amounts of protein (25 µg) were denatured 1:1 in 2X Laemmli buffer (BIO-RAD) at 95°C for 10 minutes then held on ice ...
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bioRxiv - Pathology 2021Quote: ... Protein samples were first diluted 1:1 with Laemmli sample buffer (Bio-Rad, Hemel Hempstead, UK) containing 5% β-mercaptoethanol (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... followed by secondary antibodies (1:4000 donkey anti-goat, Abcam; 1:4000 goat anti-mouse, Biorad). Immunoblots were developed using SuperSignal West Pico PLUS Chemiluminescent Substrate (ThermoFisher).
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bioRxiv - Microbiology 2021Quote: ... 20 uL cell suspension was diluted 1:1 with 0.4% Trypan Blue solution (Bio-Rad #1450021) and cells were counted by hemocytometer (RPE-1 cells ...