Labshake search
Citations for Bio-Rad :
51 - 100 of 155 citations for Degassed Water since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... we used 10µL of desalted assembly reaction products to transform competent E.coli S17-1 cells (mid-log cells, washed three times in deionized ice-cold water) by electroporation (V=1.8kV; MicroPulser, BioRad). After 1h recovery at 37°C in 1mL of LB ...
-
bioRxiv - Genomics 2022Quote: ... and then destained with deionized water for 30 min before imaging on a ChemiDoc MP imaging system (Bio-Rad).
-
bioRxiv - Microbiology 2020Quote: ... 15 % methanol (v/v) in distilled water) for 1 hour at 100 volts (Mini Trans-Blot Cell, Bio-Rad). The nitrocellulose membrane was blocked in blocking buffer (5% (w/v ...
-
bioRxiv - Cell Biology 2019Quote: ... the gels were stained with 0.4 µg/mL ethidium bromide in water for 10 min and visualized using the ChemiDocTM XRS+ (Biorad) imaging system ...
-
bioRxiv - Cell Biology 2022Quote: ... it was briefly rinsed in deionized water and immediately imaged using the ChemiDoc XRS+ Gel Imaging System (Bio-Rad) equipped with the Image Lab 4.1 software ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... together with 2.5 µl of single (1 µl assay + 1.5 µl NF water) or multiplexed (0.5 µl of each assay) PrimePCR Probe Assays (Bio-Rad) as follows with single or multiplex reactions grouped in parentheses and formatted as Gene/UniqueAssayID ...
-
bioRxiv - Microbiology 2023Quote: ... diluted to 20 ng/µL in molecular grade water and 1 µL used for Sybr Green reactions as per manufacturer’s instructions on a BioRad CFX384 (BioRad). Relative expression was calculated by ΔΔCt method to GapDH 66 ...
-
bioRxiv - Biochemistry 2023Quote: ... The gels were then transferred to a water-activated nitrocellulose membrane at 1.3A to 25V for 7 minutes (BioRad TurboBlotter ...
-
bioRxiv - Microbiology 2023Quote: ... Samples were diluted to 4 M urea with water for Bradford Assay quantification (Bio-Rad Laboratories, Inc, Hercules, CA) and trypsin digestion ...
-
bioRxiv - Developmental Biology 2023Quote: ... and aliquots were 1:50 in water prior to Bradford protein assay (Bio-Rad, Hercules, CA, USA, Cat# 5000006). Samples were then normalized to 1 mg/mL protein in 0.1% Azo prior to reduction with 30 mM DTT at 37C for 1 h and alkylation with 30 mM chloroacetamide for 45 min ...
-
bioRxiv - Cell Biology 2024Quote: ... rinsed twice with water and resuspended in 20 µL Laemmli sample buffer (Bio-Rad, 2X with 5% β-mercaptoethanol). After boiling for 5 min at 100°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... The resulting cDNA was diluted with nuclease-free water and subsequently analyzed by qPCR using iTaq Universal SYBR Green Supermix (BioRad) on a Roche LightCycler 96 instrument (Roche) ...
-
bioRxiv - Cancer Biology 2019Quote: ... DNA template (up to 8 μL) and nuclease-free water were loaded into a disposable droplet generator cartridge (Bio-Rad). After adding 70 μL of droplet generation oil (Bio-Rad) ...
-
bioRxiv - Microbiology 2020Quote: ... and destained with 30% methanol,10% glacial acetic acid in double distilled water and subjected to film by ChemiDoc systerm (BioRad).
-
bioRxiv - Molecular Biology 2019Quote: ... The pellet was dissolved in RapiGest SF (Waters) and the protein concentration was determined (Bio-Rad protein assay, Bio-Rad). The mitoribosomal lysates were frozen in liquid nitrogen and analyzed at the Proteomics core facility of the Medical University of Vienna ...
-
bioRxiv - Molecular Biology 2019Quote: ... The pellet was dissolved in RapiGest SF (Waters) and the protein concentration was determined (Bio-Rad protein assay, Bio-Rad). The mitoribosomal lysates were frozen in liquid nitrogen and analyzed at the Proteomics core facility of the Medical University of Vienna ...
-
bioRxiv - Microbiology 2022Quote: ... rinsed 3x with DI water 10 minutes each and stained overnight with agitation in QC colloidal Coomassie stain (Bio-Rad). The gel was rinsed with DI water 3x for 10 min and imaged using a ChemiDoc Touch Imaging System (Bio-Rad ...
-
bioRxiv - Bioengineering 2020Quote: 50 μL of PCR mastermix solution in Nuclease-free ultrapure water contained 60 mM Tris-HCl Buffer pH 8.8 (Bio-Rad), 200 μM dNTPs ...
-
bioRxiv - Bioengineering 2020Quote: ... 50 μL of PCR mix solution in nuclease-free water contained 60 mM Tris-HCl Buffer pH 8.8 (Bio-Rad), 200 μM dNTPs ...
-
bioRxiv - Systems Biology 2020Quote: ... cDNA was diluted in nuclease-free water and quantified using SsoAdvanced Universal SYBR Green Supermix on a CFX Connect Real-Time System (BioRad) with the following amplification scheme ...
-
bioRxiv - Molecular Biology 2020Quote: ... RT+ and RT-reverse transcription reactions were diluted 67-fold in RNase/DNase free water and RT-qPCR was performed on the CFX96 Real Time System (BioRad) using GoTaq qPCR Master Mix (Promega) ...
-
bioRxiv - Bioengineering 2021Quote: ... The RNA concentration was quantified with a nanodrop and the samples concentration were evened with nucleoside-free water (Bio-Rad). To synthesize cDNA from RNA ...
-
bioRxiv - Biochemistry 2019Quote: ... Gels were then incubated in distilled water for 10 minutes and visualized by UV transillumination (Bio-Rad ChemiDoc Imaging System). The results were quantified by ImageJ software (Schneider et al. ...
-
bioRxiv - Molecular Biology 2020Quote: ... gels were submerged into 0.5 µg/ml ethidium bromide in water for 15 minutes and imaged with Gel Doc XR+ system (Bio-Rad). Banding pattern of Mlh1+/− samples were compared to the controls to identify the allele with and without Mlh1 promoter methylation.
-
bioRxiv - Microbiology 2020Quote: ... glycine and 0.1% (w/v) SDS in distilled water) for 1 hour at 150 volts (Mini-protean vertical electrophoresis cell, Bio-Rad). The gels were stained in Coomassie stain (45% methanol ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were centrifuged at 3000g and the pellet was washed with ice-cold water twice before resuspending in 10% glycerol solution for electroporation using a GenePulser XCell (BioRad) electroporator with the pre-set bacterial protocol for E ...
-
bioRxiv - Molecular Biology 2021Quote: ... The resulting cDNA was diluted with nuclease-free water and subsequently analyzed by qPCR using iTaq Universal SYBR Green Supermix (BioRad) on a Roche LightCycler 96 instrument (Roche) ...
-
bioRxiv - Molecular Biology 2021Quote: ... The resulting cDNA was diluted with nuclease-free water and subsequently analyzed by qPCR using iTaq Universal SYBR Green Supermix (BioRad) on a Roche LightCycler 96 instrument (Roche) ...
-
bioRxiv - Molecular Biology 2021Quote: ... The resulting cDNA was diluted with nuclease-free water and subsequently analyzed by qPCR using iTaq Universal SYBR Green Supermix (BioRad) on a Roche LightCycler 96 instrument (Roche) ...
-
bioRxiv - Biochemistry 2023Quote: ... 7% acetic acid for 30 minutes at room temperature followed by two 5-minute washes in Mill-Q water for imaging on the ChemiDoc (BioRad). Alternatively ...
-
bioRxiv - Cancer Biology 2023Quote: ... Samples were diluted with distilled water to achieve equal concentrations and a loading buffer (4x Laemmli Sample Buffer, Biorad, 1610747) containing 100 mM DTT (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2023Quote: ... Recovered DNA suspended in molecular grade water was used for Sybr Green reactions (1 µL) on a BioRad CFX384 (BioRad). ChIP-qPCR primers (50-150 bp ...
-
bioRxiv - Microbiology 2023Quote: ... gels were briefly washed with water and proteins were transferred (25 V, 1.3 A, 7 min) onto nitrocellulose membranes (Bio-Rad) using Bio-Rad Trans Blot Turbo Transfer system ...
-
bioRxiv - Animal Behavior and Cognition 2023Quote: ... aliquots constituting 250 ng of RNA were diluted with nuclease-free water to a volume of 15 µL and processed using the iScriptTM cDNA Synthesis Kit (BioRad Laboratories Inc. ...
-
bioRxiv - Developmental Biology 2023Quote: ... and sample aliquots were diluted 1:50 in water before Bradford protein assay (Bio-Rad, Hercules, CA, USA, Cat# 5000006). Samples were normalized to 1 mg/mL in 0.1% Azo ...
-
bioRxiv - Molecular Biology 2024Quote: ... the SDS-PAGE gel was rinsed once in water and the GFP fluorescence was imaged in-gel using a ChemiDoc Imaging System (Biorad). The SDS-PAGE gel was then washed twice for 15 minutes in 25% isopropanol in water ...
-
bioRxiv - Microbiology 2021Quote: ... excess stain was washed away with deionized water and the gel was imaged on a ChemiDoc Touch Imaging System (Bio-Rad). DNA molecular weight standard (BioRad ...
-
bioRxiv - Molecular Biology 2021Quote: ... 10 μl of the bead-water mixture was taken for immunoblotting analysis where the beads were mixed with 2X Laemmeli Sample Buffer (Bio-Rad) supplemented with DTT and boiled at 95°C for five minutes prior to loading onto a BOLT 4-12% Bis-Tris Plus gel (Life Technologies) ...
-
bioRxiv - Pathology 2019Quote: ... 20% methanol and MilliQ water) overnight at 4°C in a wet transfer apparatus at 70 mA constant voltage (Bio-Rad). Detection of the chemiluminescence immunochemical reaction was performed on a ChemiDoc ™ XRS + Molecular Imager® (Bio-Rad ...
-
bioRxiv - Developmental Biology 2019Quote: ... and nuclease-free water controls were spotted on a pre-wetted (10X SSC buffer) nylon membrane using a Bio-Dot Microfiltration Apparatus (Bio-Rad). The blotted membrane was air-dried and UV-cross-linked at 1200 J/m2 using a UV Stratalinker 1800 (Stratagene) ...
-
bioRxiv - Neuroscience 2019Quote: ... 30 μg of protein was heated in a 70°C water bath for 10 min prior to loading into into a 4-15% CriterionTM TGXTM precast Midi protein gel (Bio-Rad) for SDS-PAGE followed by wet transfer to nitrocellulose membrane ...
-
bioRxiv - Plant Biology 2019Quote: ... qPCR analyses were performed with cDNAs diluted 50-fold with nuclease-free water using iTag Universal SYBR Green Supermix (Bio-Rad) or Brilliant III Ultra-Fast SYBR Green qPCR Master Mix (Agilent ...
-
bioRxiv - Bioengineering 2019Quote: ... Cells were then washed three times with DI water and stained with 2 ml hematoxylin (Bio-Rad Laboratories, Hercules, CA, USA) for 15 min at room temperature ...
-
bioRxiv - Genetics 2020Quote: ... twice in 200 μl nuclease free water and once in 100μl 1x SSC (150 mM NaCl, 15mM sodium citrate, pH 7.0, BIO-RAD Laboratories, Inc.) and finally resuspended in 50 μl 1X SSC ...
-
bioRxiv - Microbiology 2021Quote: ... The cDNA samples were then diluted 1:4 in water and used in quantitative real-time PCR with genespecific primers using SsoAdvanced Universal Sybr green Supermix (1725271, Bio-Rad) according to the manufacturer’s protocol (Supplementary Table 1) ...
-
bioRxiv - Cancer Biology 2022Quote: ... was used for qPCR employing 2 μL of cDNA sample and 18 μL mix of master mix (water, primers and qPCR mix) per well in 96-well plates (HSP9601, Bio-Rad), which were sealed using transparent film (MSB1001 ...
-
bioRxiv - Molecular Biology 2022Quote: ... for 30 minutes at room temperature was followed by three 5 minute washes in milliQ water before imaging on a ChemiDoc MP (Bio-Rad).
-
bioRxiv - Bioengineering 2021Quote: ... and were scanned and monitored using high-performance liquid chromatography (HPLC) (Waters, 2707, NL) equipped with an Aminex HPX-87H column (BioRad, USA) column ...
-
bioRxiv - Microbiology 2020Quote: ... The resulting cDNA was diluted 1:4 in nuclease free water and each cDNA was combined with segment specific primers (Supplementary Table 2) and Precision Melt Supermix (Bio-Rad) and analyzed by qPCR in a CFX384 Touch real-time PCR detection system (Bio-Rad ...
-
bioRxiv - Microbiology 2020Quote: ... cDNA was diluted 1:4 with nuclease-free water and used as template (4 µL per reaction) for segment-specific qPCR using SsoFast EvaGreen Supermix (Bio-Rad) in 10 µl reactions ...