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Citations for Bio-Rad :
351 - 400 of 407 citations for Cy7 Dyes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... 5 µL of sample was incubated with 195 µL of Bradford reagent (Quick Start™ Bradford 1x Dye Reagent #5000205, BioRad). Absorbance was measured at 550 nm after a 5-10 minutes incubation protected from the light ...
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bioRxiv - Microbiology 2023Quote: ... Purity of the DENV VLP was assessed by SDS-PAGE followed by Coomassie dye-based staining using QC colloidal Coomassie stain (Bio-Rad). The morphologies of DENV VLPs were analyzed at the National Institute of Infectious Disease in Japan (NIID ...
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bioRxiv - Plant Biology 2023Quote: ... 5 mM 2-mercaptoethanol) and protein concentration measured using Bradford method (Bio-Rad Protein Assay Dye Reagent Concentrate; Bio-Rad Laboratories).
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bioRxiv - Biochemistry 2024Quote: ... and 5 to 20 mM imidazole (wash-buffer) until the eluate was protein-free as judged by the lack of reaction with Bradford dye reagent (Bio-Rad). The calpain-3-titin/I81-I83 complex was eluted with 300 mM imidazole in wash-buffer ...
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bioRxiv - Biochemistry 2024Quote: Thermal unfolding of GCase was followed by thermal shift assays using SYPRO orange fluorescence as dye and a CFX connect real-time PCR system (Bio-Rad)48 ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative real-time PCR reactions were carried out using the SYBR Green PCR master mix and ROX Passive Reference Dye (Bio-Rad) with Micro-Amp Optical 384-well plates ...
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bioRxiv - Biophysics 2021Quote: ... The labeling efficiency of dye was analyzed by SDS-PAGE followed by fluorescence imaging using Gel ChemiDoc system (Bio-Rad, Hercules, CA). The concentration of SNAP-gp17 was quantified using Image Quant software normalized to standard protein markers ...
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bioRxiv - Molecular Biology 2019Quote: ... Concentrations of the purified proteins were determined by Bradford Assay using the Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad 5000006) and calculating the ratio of the samples’ absorbance at 595 nm and 472 nm ...
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bioRxiv - Molecular Biology 2020Quote: ... QPCR was performed using Precision Melt Supermix containing EvaGreen dye (cat# 172-5110) using CFX96 Touch™ Real-Time PCR Detection System (BioRad, USA). Sequences of PCR primers are listed in Table.
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bioRxiv - Biophysics 2019Quote: ... The concentration of the dialyzed protein was estimated using the Quick Start®Bradford Dye Reagent 1X (BIO-RAD Cat. #500-0205). The protein was stored at 4 °C before use.
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bioRxiv - Biophysics 2020Quote: ... 50 µl of a 10− 6 M biotinylated and 10−7 M dye-coupled oligonucleotide solution were co-incubated to anneal the oligonucleotides at 80 °C using a Thermocycler (BioRad, C1000 ThermoCycler), followed by sample cooling to 4 °C with -1 °C/min ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative PCR was performed on three biological replicates along with three technical replicates using the SYBR-green dye premixed master-mix (Thermo Fischer Scientific) in a C1000 Touch© thermal cycler (BIO-RAD). CT values were obtained using Bio-Rad CFX manager 3.1 ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell lysates were centrifuged in v-bottom 96-well plates rather than microcentrifuge tubes and total protein concentration was determined using Protein Assay Dye (BioRad #500-0006) following standard protocols ...
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bioRxiv - Biochemistry 2020Quote: ... The protein concentration of the supernatants was determined using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-rad Laboratories, Inc., CA, USA); the absorbance was measured at 595 nm with a Thermo Scientific Multiscan® GO (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... levels were quantified by real time polymerase chain reaction (RT-PCR) using SYBR® Green DNA intercalating dye and master mix (Bio-Rad) and the ABI 7900HT PCR machine ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative PCR was performed on three biological replicates along with three technical replicates using the SYBR-green dye premixed master-mix (Thermo Fischer Scientific) in a C1000 Touch© thermal cycler (BIO-RAD). CT values were obtained using Bio-Rad CFX manager 3.1 ...
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bioRxiv - Plant Biology 2021Quote: ... The PowerUp™ SYBR® Green fluorescence dye was used for quantitative gene expression analysis on CFX connect Real-time PCR detection system (Bio-Rad). The details of genes used for expression analysis and the primers are presented in Supplementary Table S3 and S4 ...
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bioRxiv - Microbiology 2020Quote: ... Immunopurified proteins were also stained with Pro-Q® Diamond phosphoprotein gel dye (Thermo) or Phospho-Tag (ABP Biosciences, MD, USA) and visualized in a ChemiDoc MP imaging system (Bio-Rad systems) under specific emission/excitation wavelengths (550/580 nm).
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bioRxiv - Genomics 2022Quote: ... and was targeted by a pre-designed assay with a HEX fluorescent dye and an Iowa Black quencher (BioRad, Assay ID: dMmuCNS420644255). The restriction enzyme ...
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bioRxiv - Biochemistry 2021Quote: ... Harvested cells were lysed in TNI lysis buffer and 10 μg of protein (as measured using Bio-Rad protein assay dye reagent) was aliquoted and precipitated via methanol/chloroform precipitation as described below ...
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bioRxiv - Plant Biology 2019Quote: ... qRT-PCR technical replicate reactions were prepared for each of the genes using the iTAq Universal SYBR Green Supermix with ROX reference dye (BioRad, Hercules, CA) per the manufacturer’s protocols with 100ng of template cDNA ...
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bioRxiv - Physiology 2021Quote: ... Protein concentration was determined in triplicate using a Bradford assay (Bio-Rad protein assay dye reagent concentrate, Bio-Rad Laboratories, Hercules, CA), against bovine serum albumin standards (BSA ...
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bioRxiv - Plant Biology 2019Quote: ... qRT-PCR technical replicate reactions were prepared for each of the gene targets using the iTAQ Universal SYBR Green Supermix with ROX reference dye (BioRad, Hercules, CA) per the manufacturer’s protocols with 20ng of template cDNA ...
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bioRxiv - Immunology 2022Quote: ... and the amount of protein was quantified by Bradford assay [31] using a colorimetric Protein Assay Dye (500-0006, Bio-Rad, USA) and Bovine Gama Globulin in known concentration for the standard curve ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the whole cell membrane pellets were resuspended in buffer and protein concentration was estimated by dye binding (Coomassie Brilliant Blue R-250; Bio-Rad, USA). The binding reaction was carried out in a final volume of 0.1 ml of Na+-containing buffer (20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was kept and the total protein concentration was determined by standard Bradford method (Bio-Rad Protein Assay Dye Reagent #5000006) using a BSA calibration curve (MP BIomedicals #160069 ...
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bioRxiv - Developmental Biology 2023Quote: ... the supernatant in a new tube received 20% glycerol and protein concentration was estimated by Bradford’s Dye Reagent Concentrate (BioRad Laboratories, Hercules, CA). For gel electrophoresis ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration was monitored periodically during the process using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, Hercules, California, USA) and concentrated to approximately 100-200 μM.
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bioRxiv - Plant Biology 2024Quote: ... The protein concentration of the filtered lysate was determined using the Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad; www.bio-rad.com) and bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2024Quote: ... the concentration of the resultant solution containing labelled αSyn was adjusted to 2 mg/mL and excess unbound dye was removed using Bio-Spin 6 size exclusion spin columns (Bio-Rad Laboratories). ATT565-αSyn was added to LUHMES cells on day six in vitro at a final concentration of 2 µM ...
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bioRxiv - Biophysics 2024Quote: ... Labeled RPA variants were separated from excess dye using a Biogel-P4 gel filtration column (Bio-Rad Laboratories; 65 ml bed volume) using storage buffer (30 mM HEPES ...
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bioRxiv - Genetics 2021Quote: ... Total protein concentration of the supernatant was measured using the Bradford assay (Bradford Reagent Protein Assay Dye Reagent, Bio-Rad, cat. number 5000006). Unboiled protein samples were then mixed with 4x Laemmli sample buffer and 100 mM dithiothreitol (DTT) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.08 of each M13 dye and 1 μL of the DNA template using a Bio-Rad thermocycler T100 (Bio-Rad, Pleasanton, CA, USA). PCR products were analyzed on an ABI 3500 genetic analyzer and sized against the LIZ500 internal standard (Applied Biosystems ...
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bioRxiv - Plant Biology 2019Quote: ... Quantitative RT-PCR was performed on a CFX384 Touch™ Real-Time PCR Detection System using SYBR Green dye (iQ™ SYBR® Green Supermix, Biorad) using the following primers ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Purification was performed at 4 °C and protein concentration after the dialysis was determined by DC protein assay (Protein Assay Dye Reagent, Bio-Rad, Hercules, CA) using BSA as standard.
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bioRxiv - Physiology 2020Quote: ... versions with an N-terminal cysteine were additionally made in order to conjugate a TMR-C5-maleimide Bodipy dye (Bio-Rad, CA, USA), to make fluorescent TMR-C5-maleimide-SPTISITAPIDVLRKTWAKENMRKQMQINREYLKNLQamide (DH37-F ...
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bioRxiv - Biochemistry 2020Quote: Protein lysate from DENV infected and non-infected Huh7 cells (10 µg of protein as measured using Bio-Rad protein assay dye reagent) was aliquoted and purified using methanol/chloroform precipitation ...
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bioRxiv - Plant Biology 2021Quote: ... 1976) samples mixed with loading dye were loaded on the polyacrylamide matrix (Mini-PROTEAN® Tetra System 12-0625 BioRad, Hercules, California, USA). To detect the separated ADH isoforms ...
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bioRxiv - Genetics 2021Quote: ... Samples were then centrifuged at 4°C for 5 min at 20,000 g and protein concentrations in the supernatants were measured using the Bradford assay (Bio-Rad Protein Assay Dye). Aliquots equivalent to 1 μg and 3 µg protein were separated on NuAGE 12% Bis-Tris Gels using MOPS 1X (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell viability dye Viva Fix 498/521 (#1351115) and mouse anti-human CD138 Alexa Fluor 647 (#MCA2459A647) were obtained from Bio-Rad (Hercules, California). CD138 magnetic beads were procured from Miltenyi Biotec (Auburn ...
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bioRxiv - Microbiology 2022Quote: ... Cells were lysed using RIPA buffer and total protein concentration was quantified using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, 500-0006). Twenty micrograms of protein were incubated in Laemmli sample buffer at 95°C for 5 min and subjected to electrophoresis on a 3-15% gradient polyacrylamide gel ...
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bioRxiv - Neuroscience 2022Quote: ... PCR Reactions were then performed in triplicate (3n) using a Dye Based iTaq Universal SYBR® Green Supermix (Bio-Rad, Hercules California #1725121). The Thermal Cycling protocol was performed on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged at 16,000Xg for 30 min at 4°C then protein concentrations were determined by Quick Start™ Bradford 1x Dye Reagent (Bio-Rad #5000205). For each sample ...
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bioRxiv - Cell Biology 2024Quote: ... The protein concentration in samples was determined with the Bradford assay using a Protein Assay Dye Reagent (#500-0006, Bio-Rad, Richmond, CA) with optical density reading at 595 nm (OD595) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The concentration of total protein mite extracts was determined using the Quick Start Bradford Protein Assay (Quick start Bradford 1x dye reagent, Bio-Rad, Cat# 500-0205), with Bovine Serum Albumin (Sigma-Aldrich ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... The triglyceride content was normalized to the total protein content of the flies estimated by Quick Start™ Bradford 1X Dye Reagent (BioRad, Cat. # 500-0205). Freshly emerged flies feed less and possess larval triglyceride energy storage ...
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bioRxiv - Biochemistry 2020Quote: To visualize protein expression 1 μL of the TXTL reactions was mixed with 2 μL of water and 3 μL of 2x Laemmli loading dye (Bio-Rad, Hercules, CA, USA) and incubated at 90°C for 3 min ...
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bioRxiv - Cell Biology 2021Quote: ... Fluorescent dye-conjugated secondary antibodies were used at 1:3000 and the proteins were detected using ChemiDoc MP Imaging System (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Biochemistry 2022Quote: ... 10μL of each time point were mixed with RNA loading dye and heated to 95°C before loading on a 1.0 cm well PAGE casting plate (Bio-Rad Laboratories, Mississauga, ON, Canada). Samples were run on a 7.5% Urea PAGE gel for 30 minutes at 300V in 0.5X TBE buffer ...
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bioRxiv - Plant Biology 2021Quote: ... Protein quantities were measured by Bradford assays according to the manual provided with the Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad Laboratories; Hercules, USA). Proteins were immunoprecipitated by 50 µl Anti-HA MicroBeads (Miltenyi Biotec ...