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Citations for Bio-Rad :
51 - 100 of 5902 citations for Cow Ataxin 10 ATXN10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: The 100µl plasma was inactivated by heating at 56°C for 30 min 10 .60µl plasma was used for albumin and globulin depletion using an Aurum serum mini kit (BioRad, USA). The six depleted plasma samples were used for SWATH-MS analysis ...
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bioRxiv - Plant Biology 2021Quote: ... 10% (Bio-Rad). For each sample ...
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bioRxiv - Biophysics 2024Quote: ... 10% acrylamide (BIORAD), 0.1% SDS ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We used a precast gel (Biorad, 10% Mini-Protean, 10 wells) to load 15 µl of sample per well and a Biorad PAGE system for 5 min at 50 V ...
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bioRxiv - Immunology 2021Quote: ... TMB substrate was added to stop the reaction before performing the reading at 450 nm in the ELISA plate reader (iMark Microplate Reader, Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... 5 μL of DNase-treated samples were reverse transcribed in a final volume of 10 μL using the iScript cDNA synthesis kit (Bio-Rad) following the manufacturer’s protocol and diluted 5-fold in nuclease-free water before qPCR ...
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bioRxiv - Biochemistry 2021Quote: ... Cell lysates were boiled for 10 min and quantified with the DC protein assay kit following the microplate protocol provided by the company (Bio-Rad). Whole cell lysates (15-40μg protein ...
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bioRxiv - Microbiology 2021Quote: ... 0.5 µg of each RNA sample was used in a 10-µL reverse transcription reaction set up using the iScript Select cDNA synthesis kit (Bio-Rad), together with gene-specific reverse primers (Table S2 ...
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bioRxiv - Developmental Biology 2021Quote: ... eluted total RNA in 10 μl of Elution buffer and reverse transcribed RNA in 20 μl using the iScript cDNA synthesis kit (Bio-Rad). We incubated the samples for 5 min at 25°C ...
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bioRxiv - Genomics 2021Quote: ... According to kit instruction 10 μl reaction system was prepared and q-PCR was operated using CFX96 Real-Time System (BIO-RAD). For data analysis ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... BP176-100) and run on a 10% SDS-PAGE using the TGX Stain-free FastCast Acrylamide Kit (Bio-Rad Cat# 1610183) and the Mini-PROTEAN system (Bio-Rad Cat# 1658006) ...
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bioRxiv - Microbiology 2022Quote: ... methanol in 1X TBE buffer with 10% (v/v) acetic acid) and RNA was visualised using the Silver Stain Plus Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA was synthesized in duplicate using 500 ng RNA in 10 uL iScript Advanced cDNA Synthesis Kit (Bio-Rad, 172-5038). Reactions were diluted 1:5 in water ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was purified with QIAquick® PCR purification kit and 1-10 ng of immunoprecipitated or input DNA were amplified with iQ™ SYBR Green Supermix (BioRad) using a Real-Time PCR machine (BioRad IQ™ 5) ...
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bioRxiv - Microbiology 2023Quote: ... 35 µg of lysate was loaded on 10% SDS-PAGE after quantifying the protein concentration using Bradford assay kit (Bio-Rad). After transferring bands to nitrocellulose membrane Ponceau S staining was performed for checking equal loading of protein on SDS-PAGE ...
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bioRxiv - Plant Biology 2024Quote: ... 10 μL first-strand cDNA was synthesized using 500 ng RNA with iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad, USA) following the manufactureŕs instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and 500 ng RNA was reverse transcribed into cDNA in a 10 μl reaction using the iScript cDNA synthesis kit (Bio-Rad). cDNA was then diluted 1:10 and 4 μl was used for quantitative RT-PCR using the LuminoCt SYBR Green master mix on a Roche LightCycler 480 machine ...
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bioRxiv - Zoology 2021Quote: The quantitative determination of IFN-ɣ in buffalo serum was assayed by a commercial bovine IFN gamma sandwich ELISA test (Bio-Rad) following the manufacture’s specifications ...
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bioRxiv - Immunology 2020Quote: ... RBD-specific antibody titres in oral and nasal swab fluids were determined by ELISA as detailed above except that the conjugated secondary antibody was replaced with either goat anti-porcine IgG HRP (Bio-Rad Antibodies) at 1:20,000 dilution in PBS with 1% (w/v ...
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bioRxiv - Plant Biology 2021Quote: ... and treated aliquots amounting to 10 ng RNA were used for cDNA synthesis (iScript™ cDNA Synthesis Kit, BioRad, Hercules, CA, USA). Quantitative real-time PCR was carried out using a StepOnePlus (Applied Biosystems ...
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bioRxiv - Cancer Biology 2020Quote: The cell harvesting and extraction of proteins followed the method described previously.10 A protein assay kit (Bio-Rad, Hercules, CA, USA) was used to determine protein concentrations ...
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bioRxiv - Developmental Biology 2021Quote: ... Five hundred ng of total RNA (from 21EM15 cells or mouse lenses) or 10 ng of total RNA (from explants) were reverse transcribed using the iScript cDNa Synthesis Kit (Bio-Rad, CA) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and 10-14h after egg laying and reverse transcribed using oligo-dT primers with a One-Step RT kit (Bio-Rad 1725140). The coding sequence for each gene encoding a gene of interest was amplified from a cDNA template using Phusion High Fidelity DNA polymerase (Thermo Fisher F-530XL) ...
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bioRxiv - Cancer Biology 2021Quote: ... and 20 μg of proteins per sample were resolved in 10 or 12% gels (TGX™ FastCast™ Acrylamide kit, Bio-Rad) via electrophoresis ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting pellets were resuspended in homogenization buffer and centrifuged again at 9000 g for 10 min at 4°C and protein concentration was measured using the Quick Start Bradford Protein Assay Kit 1 (Bio-Rad 5000201). Mitochondrial respiration was measured in Mitochondrial Assay Solution (MAS ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein measurements were performed using a BCA kit (Pierce) and 150 µg was loaded on a 10-20 % tris/tricine SDS-page gels (Bio-Rad Laboratories) and blotted onto 0,2 µm nitrocellulose membrane (Bio-Rad Laboratories) ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged 10 minutes at 13,000 x g and the supernatant was quantified using a DC assay kit (Bio-Rad, Hercules, California). 200 µg of total protein were diluted into a total of 1.5 mL Array Buffer and rocked over the pre-blocked array membrane containing various total RTK antibodies overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were centrifuged at 4°C at 20,160 x g for 10 minutes and supernatants were collected for protein measurement using the DC Protein Assay Kit (Bio-Rad, 5000111). Equal amounts of protein were loaded to run the gels (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Samples were heated for 10 min at 95°C before loading 10 µl onto a 10% polyacrylamide precast mini-PROTEAN TGX gel (Bio-Rad). After electrophoresis the gel was washed twice with distilled water ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Absorption was read at 450 nm with wavelength correction set to 540 nm using an ELISA plate reader (Bio-Rad, Hercules, CA, USA). Intra- and inter-assay coefficients of variation were <10% for all EIAs.
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bioRxiv - Molecular Biology 2021Quote: ... The plates were incubated at 37°C for different time points (0,4hr,12hr,24hr,72hr) and was measured on an ELISA reader (Bio-Rad, Hercules, CA, USA) at a wavelength of 570 nm and O.D was recorded.
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by electrophoresis on a 10% SDS polyacrylamide gel and visualised with Silver Stain Plus Kit (Bio-Rad Laboratories AB, Sweden) according to manufacturer’s modified silver stain protocol.
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bioRxiv - Biochemistry 2023Quote: ... 10% SDS and 250 mM Tris-Cl pH 6.8) for 10 minutes prior to SDS-PAGE separation (Bio-Rad, 10% acrylamide, 150 V) for subsequent analysis by either Coomassie staining or blotting on nitrocellulose ...
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bioRxiv - Microbiology 2021Quote: ... boiled at 95°C for 10 min and loaded on 10% gels (Bio-Rad) for electrophoresis ...
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bioRxiv - Neuroscience 2020Quote: ... Each sample was mixed 1:1 (10 μl + 10 μl) with Laemmli buffer (BioRad) and boiled at 95°C for 5 min before loaded onto a 4-12% SDS/PAGE gel and run at 200 V before electrotransferred to a membrane using Transblot-Turbo Transfer system (BioRad) ...
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bioRxiv - Physiology 2021Quote: ... Precast 10% or 4-20% mini-PROTEAN TGX 10-well gels (Bio-Rad, 4561034) were used for protein separation electrophoresis ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Proteins (10-50 µg) were loaded onto 10% sodium dodecyl sulfate polyacrylamide gel (BioRad), separated by electrophoresis (SDS-PAGE) ...
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bioRxiv - Cell Biology 2020Quote: ... 10%-gels (Bio-Rad, Bulletin_6201) were cast in a PROTEAN Plus multi casting chamer (Bio-Rad) ...
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bioRxiv - Developmental Biology 2022Quote: ... and 10% donkey serum (Biorad) for three hours at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... 10% Criterion TGX gels (BioRad) in Tris-glycine-SDS running buffer or 3-8% Criterion XT Tris-acetate gels (BioRad ...
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bioRxiv - Cancer Biology 2021Quote: ... dimethyl sulfoxide (DMSO) was added and the absorbance of each well was measured at 490 nm using an ELISA microplate reader (Bio-Rad, Hercules, CA, USA) (33).
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bioRxiv - Physiology 2019Quote: ... Defined amounts of mRNAs at 10-fold dilutions were amplified via real-time PCR using iTaq™ Universal SYBR® Green One-Step kit (Bio-Rad, Italy) using the following RT-PCR conditions ...
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bioRxiv - Plant Biology 2021Quote: ... 10 μg of microsomes were fractionated using 10% precast SDS-PAGE gel electrophoresis (Bio-Rad). After staining with Coomassie blue (R250 ...
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bioRxiv - Immunology 2024Quote: ... diluted 1:8000 in PBT for 1 h at 37 °C before the ELISA was read out using ABTS and H2O2 at an iMark Microplate Absorbance Reader (Bio-Rad Laboratories, Inc., CA, USA). ACPA IgG levels were quantified based on an in-house standard of pooled RA patient plasma using the Microplate manager software MPM-6 (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2022Quote: ... 10 μL was injected onto a ENrich size-exclusion chromatography 650 10 × 300 column (Bio-rad) pre-equilibrated in 20 mM HEPES pH 7.4 100 mM NaCl ...
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bioRxiv - Physiology 2022Quote: ... 10-20 µg protein samples in SDS-PAGE were resolved in 7.5% -10% gels (Bio-Rad) and transblotted onto polyvinylidene fluoride membranes (Millipore) ...
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bioRxiv - Physiology 2022Quote: ... with 10% mercaptoethanol (Bio-Rad, 1610710) was heated to 95⁰C for 5 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... 7.5% or 10% (Bio-Rad, 1610173TA) at 120 V ...
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bioRxiv - Biochemistry 2022Quote: ... loaded onto gels (10% Bio-Rad Mini-PROTEAN® TGX Stain-Free™ Precast Gels ...
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bioRxiv - Cell Biology 2021Quote: ... and 9:10 laemmli (Bio-Rad)) ...