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Citations for Bio-Rad :
51 - 90 of 90 citations for Clostridium Difficile Toxin B Ribotype 078 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and sealed with adhesive Microseal ‘B’ PCR Plate Sealing Film (Bio-Rad Cat. No. MSB1001). Each biological replicate was analyzed in duplicate together with no reverse transcriptase and no template controls ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Samples were then run on either 4-15% (a) or 8-16% (b) precast gels (Biorad), transferred onto 0.1μm nitrocellulose membranes and used for immunoblot detection ...
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bioRxiv - Physiology 2019Quote: ... After loading wells were sealed with Microseal® ‘B’ seal (Bio-Rad Laboratories, the United Kingdom) avoiding that air bubbles ...
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bioRxiv - Immunology 2020Quote: cDNA was generated from sorted B cells by adding 4µl of iScript (Bio-Rad Cat# 1708891) and cycling according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... cDNA synthesis from RNA was completed using 5x iScript RT Supermix (Bio-Rad, Catalog #L001404 B) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Single antigen-binding B cells were sorted using an S3 cell sorter (Bio-Rad, Hercules, CA), and deposited into a PCR tube with 5 μL lysis buffer (Takara Bio ...
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bioRxiv - Biochemistry 2024Quote: ... 2) Arm B was purified on an ENrich™ SEC 650 24 mL column (Bio-Rad), and 3 ...
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bioRxiv - Physiology 2021Quote: ... RNA was transferred onto a nylon membrane (Hybond-N; Biodyne B) using Trans-Blot Turbo (Bio-Rad) at 25 V for 30 min ...
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bioRxiv - Synthetic Biology 2023Quote: ... Reactions were performed using a 96 well plate with an optically clear microseal ‘B’ film (Bio-Rad). Three technical replicate reactions were performed for each biological replicate ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then measured to 20μg total protein in 2x Laemmli buffer containing 10% b-mercaptoethanol (Bio-rad), heated at 70°C for 10min and loaded into 4-20% precast gels (Bio-rad ...
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bioRxiv - Molecular Biology 2020Quote: ... After 2 weeks cells were stained by the sulforhodamine B and photographed by E-Gel Imager (Bio-Rad). The cloning numbers in each group were counted to calculate the inhibition rate.
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bioRxiv - Immunology 2019Quote: ... Endotoxin was removed by passing over two polymyxin-B columns (Affi-Prep Polymyxin Resin; Bio-Rad, Hercules, CA). Additionally ...
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bioRxiv - Biochemistry 2021Quote: ... in thin-walled 96-well PCR plates with transparent Microseal® ‘B’ seals (Bio-Rad, Hercules, California, USA). The temperature ramp was between 25 °C and 94 °C with a 0.5 °C increase per cycle for 140 cycles and a cycle duration of 30 seconds ...
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bioRxiv - Plant Biology 2021Quote: ... The membrane was incubated with an anti-cardosin B antibody (Antibody generated by HuCAL technology, Bio-Rad, USA) as described in 16 diluted in PBS-T (0.4 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... primary B cells were activated as above and then lysed with 4X Laemmli buffer with beta-mercaptoethanol (BioRad) to a final concentration of 1X ...
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bioRxiv - Physiology 2023Quote: ... Individual cDNA samples were run in duplicate using iTaq Universal SYBR Green Supermix (Bio-Rad, Catalog #L001752 B), with a final volume in each well equal to 15uL ...
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bioRxiv - Biophysics 2023Quote: ... Plates were sealed with a quantitative PCR adhesive optical seal sheet (Microseal ‘B’ Adhesive Sealing Films, BIO-RAD) and then spun at 1000 rpm for 1 min to remove bubbles ...
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bioRxiv - Biochemistry 2020Quote: ... In 384-well plates (Hard-Shell 384-well microplate and Microseal B clear using an adhesive seal (Bio-Rad)) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and 15μg protein in 5x Laemmli buffer with 5% b-mercaptoethanol was loaded on Mini-PROTEAN TGX Precast Gels (BioRad). Gel lanes were cut into three sections for peptide extraction ...
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bioRxiv - Immunology 2019Quote: ... After quantifying the optimal virus-to-erythrocyte concentration (4HA units), serial two-fold dilutions of Fc, control IVIG (GammaGard, Baxter Healthcare) and polyclonal goat anti-influenza B (Biorad) were prepared ...
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bioRxiv - Biochemistry 2021Quote: ... The membrane was first blocked with B-TBST (TBS buffer with 0.05% Tween-20 and supplemented with 5% blotting grade non-fat dry milk; Bio-Rad) for 1 hr at room temperature and subsequently blotted with the primary antibodies in B-TBST overnight at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... plants were irradiated with UV-B lamps using fixtures mounted 30 cm above the plants (2 W m−2 UV-B and 0.6 W m−2 UV-A, Bio-Rad ChemiDoc™XRS UV-B lamps ...
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bioRxiv - Microbiology 2024Quote: ... software in 0.2 ml 96-well PCR plates (dot scientific inc.) sealed with transparent Microseal B Adhesive Sealer (Bio-Rad). Data were analyzed using the comparative Ct (2-ΔΔCt ...
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was blocked with B-TBST (TBS buffer with 0.05% Tween-20 and 5% blotting grade non-fat dry milk; Bio-Rad) for 1 hr at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... The membranes were then blocked with B-TBST (TBS buffer with 0.05% Tween-20 and supplemented with 5% blotting grade non-fat dry milk; Bio-Rad) for 1 hr at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... The membranes were then blocked with B-TBST (TBS buffer with 0.05% Tween-20 and supplemented with 5% blotting grade non-fat dry milk; Bio-Rad) for 1 hr at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... and incubated with 1:200 rabbit-anti NF-B p65 polyclonal antibody (Pierce) (2 h) and 1:5000 Goat anti-rabbit IgG (H+L)-HRP conjugate (Bio-Rad) (1 h) ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Protein concentration was quantified using a BCA assay and loaded at 25- 50ug in 1x Lamelli Buffer with B-mercaptoethanol onto a 4-15% TGX protean gel (Bio-Rad). In some experiments to achieve greater separation to detect the N-truncation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... SYBR Green qPCR was performed using a ViiA7 (A&B Biosystems, Waltham, MA) with iTaq Universal SYBR Green One Step Kit (BioRad, #1725151) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 μL re-suspended Fraction B pellets were run 1 cm onto an Any kD Mini-Protean TGX gel (Bio-Rad) followed by Coomassie Blue staining ...
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bioRxiv - Microbiology 2023Quote: ... PBMCs isolated from two donors were infected with GFP-EBV for 28 days in 6-well plates and subsequent B-cells blasts were photographed using using a ZOE Fluorescent Cell Imager (BIO-RAD). Using similar strategy ...
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bioRxiv - Cell Biology 2023Quote: ... proteins incubated with Mal-B were electrotransferred to PVDF membranes using a Trans-blot SD semi-dry transfer cell (Bio-Rad). Biotin incorporation was assessed by incubating blots with HRP-streptavidin (1:1000 v/v ...
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bioRxiv - Neuroscience 2022Quote: ... macrophages were plated in a 24-well plate containing cover slips and incubated with the Magic Red cathepsin B substrate (1/250) (Bio-Rad, ICT938) for 2h ...
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bioRxiv - Plant Biology 2019Quote: ... All reactions were run in hard-shell skirted 96-well plates with a white top and clear wells and sealed with Microseal ‘B’ adhesive film (BioRad, Hercules, CA). A CFX-96 detection system (BioRad ...
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bioRxiv - Cancer Biology 2020Quote: ... Wells were filled with E3 containing 0.28ug/mL Tricaine-S and the plate sealed with a PCR microseal (BioRad Microseal ‘B’ Film, #MSB1001). Up to 45 larval zebrafish per experiment were imaged in parallel on a GE IN Cell Analyzer 6000 every 15-20 minutes for 24-30h ...
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bioRxiv - Plant Biology 2022Quote: ... CLso mature effectors (without signal peptide) were amplified from haplotype A and B infected tomatoes genomic DNA under standard PCR conditions using iProof (Biorad, Supplementary Table 1). Annealing temperatures of primer pairs were calculated using FastPCR (Kalendar et al. ...
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bioRxiv - Immunology 2020Quote: ... labelled with viability stain and surface antibodies as above and fixed and permeabilised using Leucoperm A and B reagents (Bio-Rad, Watford, UK). Cells were labelled intracellularly with antibodies (Table S2 ...
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bioRxiv - Systems Biology 2023Quote: ... resolved in a 4-12% Invitrogen B-T gel using MOPS buffer and transferred onto a polyvinylidene difluoride (PVDF) membrane using BioRad transblot (Bio-Rad, Hercules, CA, USA). The membrane was blocked with 5% skim milk ...
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bioRxiv - Plant Biology 2022Quote: ... was chosen as a target for the amplification of CY phytoplasma DNA using the primer pair rp(I-B)F2/rp(I-B)R2 (Lee et al., 2003; Pagliari et al., 2017) and a CFX96 real-time PCR detection system (Bio-Rad Laboratories, Richmond, CA, USA). A standard curve was established by 10-fold serial dilutions of a plasmid DNA containing the 1260 bp ribosomal protein fragment from CY phytoplasma ...
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bioRxiv - Immunology 2021Quote: ... with 0.5 μl of b-mercaptoethanol per well and heated at 70°C for 10 minutes before separation on a polyacrylamide gel (Bio-Rad Mini-PROTEAN TGX Gel 4-15%) and transferred to a PVDF membrane ...