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Citations for Bio-Rad :
1 - 50 of 10000+ citations for Charged Multivesicular Body Protein 2b CHMP2B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Proteins were purified by Nickel charged Immobilized Metal Affinity Chromatography (BioRad, CA). The affinity tags were removed during overnight incubation with SENP (made in-house) ...
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bioRxiv - Neuroscience 2023Quote: ... the His-FUS proteins were mixed with Profinity IMAC Ni2+-charged resin (Bio-Rad) for 30 min at 20 °C ...
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bioRxiv - Cell Biology 2023Quote: ... The recombinant protein was purified using the Ni-NTA (Biorad IMAC Ni-Charged Resins) affinity chromatography under denaturing conditions ...
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bioRxiv - Plant Biology 2022Quote: ... Ni-charged (Bio-Rad, 1560133). The eluted protein concentration was estimated using a Bradford assay (Quick Start™ Bradford 1x Dye Reagent #5000205 ...
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bioRxiv - Biochemistry 2021Quote: ... Soluble proteins were extracted by using Profinity IMAC Ni-charged resin (Bio-Rad, Hercules, CA) as recommended by the manufacturer ...
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bioRxiv - Biophysics 2022Quote: ... His6-tagged proteins were purified using an Ni-NTA affinity column (Profinity IMAC Ni-Charged, BioRad).
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bioRxiv - Biochemistry 2023Quote: ... The DBC1354-396 protein was collected using 5mL Bio-Scale Mini Nuvia IMAC Ni-charged Cartridges (Bio-Rad). Digestion efficacy was assessed with SDS-PAGE analysis ...
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bioRxiv - Microbiology 2023Quote: ... His-tagged proteins in the cleared lysate were purified using Profinity™ IMAC Nickel Charged Resin (Bio-Rad) and eluted in 50 mM sodium phosphate buffer with 300 mM NaCl pH 8 and 300 mM imidazole (Fischer Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... His-tagged recombinant protein was purified using Ni-NTA purification with the help of Ni-charged resins (Bio-Rad). Purified protein was further used for kinase assay ...
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bioRxiv - Synthetic Biology 2021Quote: ... we manually introduced Ni-charged profinity resin (Bio-Rad) into the channels before use ...
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bioRxiv - Molecular Biology 2020Quote: Following electrophoretic separation, oligonucleotide-protein complexes were transferred by electro-blotting (330 mA, 1 h) onto specific positively charged nylon membranes (Bio-Rad) and detected using an anti-digoxigenin antibody conjugated with alkaline phosphatase.
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bioRxiv - Microbiology 2023Quote: ... containing 500 μl of profinity IMAC resin Ni-charged (BioRad) and mixed for 30 min rotating at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified lysates were incubated with nickel-charged Profinity IMAC resin (Biorad, CA) for one hour at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Profinity immobilized metal affinity chromatography (IMAC) Ni-charged Resin was from Bio-Rad; all IMAC buffers contained 20 mM Tris HCl pH 8 ...
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bioRxiv - Biochemistry 2023Quote: ... and loaded onto 2 mL Nuvia IMAC Ni-charged resin (Bio-Rad Laboratories). The resin was washed with 10 column volumes of buffer containing 5 mM imidazole ...
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bioRxiv - Microbiology 2023Quote: ... Resulting supernatants were mixed with Ni-charged resin beads (Bio-Rad Profinity IMAC) to purify the His-tagged reductases ...
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bioRxiv - Molecular Biology 2019Quote: ... Duplex-bound complexes were transferred onto Zeta-Probe positively-charged nylon membranes (Bio-Rad) by semi-dry transfer at 25 V for 20 min ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting supernatant was loaded onto the nickel-charged Profinity IMAC Resin (Bio-Rad, USA) and washed with 15 column volumes (CV ...
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bioRxiv - Molecular Biology 2022Quote: ... Clarified lysate was bound to a 5 ml Mini Nuvia IMAC Ni-Charged column (Bio-Rad). The resin was washed extensively with a solution of 40 mM Tris pH 8.0 ...
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bioRxiv - Developmental Biology 2021Quote: ... for fat bodies and Bradford standard assay (Bio-Rad 500-0006) for muscles ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein-antibody complexes were collected by incubating protein extracts with 20 μl SureBeads Protein G magnetic beads (Bio-Rad) for 30 min at room temperature on a rocking shaker ...
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bioRxiv - Biochemistry 2020Quote: ... and the supernatant then batch bound overnight at 4 °C to nickel-charged IMAC resin (Profinity, BioRad) which had been equilibrated with LC Lysis Buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Antibody-bound DNA was recovered using the Protein A or Protein G magnetic beads (Bio-Rad), washed similarly as DRIP samples and treated with Proteinase K and RNAse after elution ...
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bioRxiv - Microbiology 2024Quote: ... Supernatants of cell lysates were passed through a nickel bead column (Profinity IMAC Ni-Charged Resin, Bio-Rad) to allow binding of lm_ApbE-6xHis ...
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bioRxiv - Molecular Biology 2020Quote: ... including unmodified antibodies and a protein ladder (Bio-rad, #1610375) were also used ...
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bioRxiv - Immunology 2021Quote: ... RMA-S (H-2b) was transfected with pcDNA3.1-HHD-A24 by electroporation (Gene Pulser Xcell, Bio-Rad, Hercules, CA), and cloned by the FACSAria II cell sorter (BD Biosciences ...
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bioRxiv - Biochemistry 2020Quote: ... and the supernatant was mixed with urea (0.5 M final concentration) before batch binding overnight at room temperature (RT) to nickel-charged IMAC resin (Profinity, BioRad) that had been previously equilibrated with the DARET Lysis Buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Bioscale Nuvia-IMAC Ni-charged and ENrich SEC70 and SEC650 10/300 size exclusion columns were purchased from Biorad, USA and used with BioRad NGC Quest plus FPLC system ...
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bioRxiv - Biochemistry 2023Quote: ... The soluble fraction was loaded onto a 5 mL Bio-Scale Mini Nuvia IMAC Ni-charged Cartridges (Bio-Rad) which was pre-equilibrated with 5 column volumes (CV ...
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bioRxiv - Biochemistry 2024Quote: ... The enzyme was purified using a 5-mL Ni-charged EconoFit Nuvia IMAC Column (Bio-Rad, Hercules, CA, USA) and a linear gradient of 10 - 250 mM imidazole in binding buffer (100 mL in total at 2.5 mL/min flow rate) ...
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bioRxiv - Neuroscience 2022Quote: ... The following antibodies were used: rat anti-myelin basic protein (MBP, 1:100, Bio-Rad antibodies MCA409S), mouse anti-myelin-associated glycoprotein (MAG ...
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bioRxiv - Cell Biology 2023Quote: ... Concentrated conditioned medium was fractionated by size exclusion chromatography (SEC) by application to Sepharose CL-2B resin (17014001, Cytiva) stacked in a disposable Econo-Pac column (7321010, Bio-Rad). The column was eluted with PBS and 0.5 ml fractions were collected ...
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bioRxiv - Cell Biology 2024Quote: ... The larvae body was immediately snap-frozen in an empty 96-well plate (Biorad; #12001925) on dry ice and stored at -80°C until sample preparation ...
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bioRxiv - Neuroscience 2020Quote: ... Depending on the primary antibody separated proteins were transferred to nitrocellulose (BioRad) or ...
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bioRxiv - Immunology 2022Quote: ... Antibodies were eluted from Protein G beads in chromatography columns (Bio-Rad) after centrifugation and washing with PBS using 0.1 M glycine (pH=3.0 ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were expressed in TOP10 competent cells (Themo Fisher Science) and were purified using a Nuvia IMAC Nickel-charged column (Bio-Rad) on an NGC Quest chromatography system (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: ... the lysate was loaded onto a pre-equilibrated Ni2+-charged Bio-Scale Mini Nuvia IMAC Cartridge (Bio-Rad Laboratories, Hercules, California), washed with 30 mM imidazole and eluted with a fractioned gradient from 30 mM – 350 mM imidazole over 20 CV.
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bioRxiv - Synthetic Biology 2020Quote: Samples with equal amount of RNA were applied onto a positively charged membrane using the Bio-Dot Microfiltration Apparatus (Bio-Rad). The membrane was placed on the gasket and wetted with 2x SSC ...
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bioRxiv - Molecular Biology 2019Quote: ... The resolved genomic DNA was then transferred to the positively charged nylon membranes (Hybond-N+, Amersham Pharmacia Biotech) using a model 785 vacuum blotter system (Bio-Rad). The Bar amplified fragment (Table 1 ...
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bioRxiv - Microbiology 2022Quote: ... DNA/protein complexes were transferred to a Hybond-N+ positively charged nylon membrane (Amersham Pharmacia Biotech) using an electroblot transfer system (Bio-Rad) and cross-linked with short-wave UV light in a GS gene linker UV chamber (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... were added with 2X Laemmli buffer and were resolved on SDS-PAGE followed by their transfer on methanol charged Polyvinylidene fluoride (PVDF) membrane using semidry transfer system (Biorad, USA). Membrane was further treated using 5% skimmed milk in PBST for 1 hour and then probed with primary antibody and incubated overnight at 4[ ...
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bioRxiv - Plant Biology 2024Quote: Each clarified lysate was incubated with 500 µL bed volume of ProfinityTM IMAC Ni-Charged Resin (156-0135, Bio-Rad, USA) in Poly-Prep® Chromatography Columns (731-1550 ...
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bioRxiv - Biophysics 2023Quote: ... Solubilized protein was cleared by ultracentrifugation at 46,400 g and purified by metal affinity chromatography using a Nickel-charged EconoFit Nuvia IMAC column (Bio-Rad, USA). Protein was eluted in 20 mM HEPES ...
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bioRxiv - Neuroscience 2022Quote: ... Beads were then transferred to fresh low- protein binding tubes and antibody-protein complexes were eluted by incubating in 60 μL 1x Laemmli Sample Buffer (Biorad, cat ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were detected using HRP-conjugated antibodies and enhanced chemiluminesence reagent (Bio-Rad). Band intensities were quantified using ImageJ software.
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bioRxiv - Immunology 2022Quote: ... antibodies bound to Protein G beads were eluted in chromatography columns (Bio-Rad) using 0.1 M glycine (pH=3.0 ...
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bioRxiv - Neuroscience 2019Quote: ... Protein bands were visualized using horse radish peroxidase (HRP)-coupled secondary antibodies (BioRad) and SuperSignal West Dura solution as substrate (Thermofisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein bands were detected using Clarity Chemiluminescent HRP Antibody Detection Reagent (Bio-Rad) and visualized with a chemiluminescence reader (Fusion SL ...
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bioRxiv - Microbiology 2022Quote: ... His6 tagged proteins were detected with anti-His6 primary antibody (Pierce; 1:6,000) and antimouse secondary antibody (Biorad; 1:10,000). GroEL was used as cytoplasmic control and detected as above ...
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bioRxiv - Plant Biology 2023Quote: ... Native protein extracts from E.coli or Synechococcus ΔCS were incubated in IMAC HEPES binding buffer supplemented with potential effector molecules and Ni-charged iminodiacetic acid Profinity™ IMAC Resin (Bio-Rad, USA) on a low speed rotator for 1 h at 23°C ...