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Citations for Bio-Rad :
51 - 100 of 9557 citations for Borrelia burgdorferi C p Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The denatured lysate was then clarified by centrifugation (13200 rpm, 10 min at 4°C) and protein concentration was determined using the DC™ Protein Assay (Bio-Rad). Either equal amounts or a serial dilution of the lysates from various strains were resolved using SDS-PAGE and transferred onto a polyvinylidene difluoride membrane ...
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bioRxiv - Biochemistry 2023Quote: ... DsbAΔSP was oxidized with a 10-fold molar excess of AT-2 and roGFP2 was reduced with a 10-fold molar excess of DTT for 10 min at 20 °C and then the oxidant or reductant were removed using Micro Bio-Spin™ P-30 gel columns (BioRad, Feldkirchen, Germany, #7326223) or NAP™-5 columns (Cytiva ...
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bioRxiv - Microbiology 2022Quote: The concentrations of the 37°C samples were determined with a DC Protein Assay (BioRad). A volume corresponding to 50 μg of the 37°C sample was used for further analysis ...
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bioRxiv - Cancer Biology 2022Quote: ... Lysate was clarified by centrifugation at 4 °C and protein quantified using bradford (Bio-Rad) assay ...
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bioRxiv - Plant Biology 2023Quote: ... for 1 h at 4 °C and incubated with protein-G magnetic beads (Bio-rad) for 0.5 h at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... and electrotransfer to Immobilon P (Biorad, France), the membrane was incubated with primary antibodies and then HRP-labelled secondary antibodies (Jackson ImmunoResearch Laboratories) ...
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bioRxiv - Neuroscience 2020Quote: ... 15 min at +4°C) and the supernatant was used to measure the protein concentrations using the Lowry method (Biorad DC protein assay, Bio-Rad, Richmond, CA) (Lowry et al ...
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bioRxiv - Microbiology 2021Quote: ... and phospho-Akt (p-Akt) were quantified in 10 μg of total protein using the Bio-Plex Pro magnetic phosphoprotein detection assay (Bio-Rad, United Kingdom). Phosphoprotein expression was normalized to β-actin and expressed as fold-change relative to the uninfected (UI ...
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bioRxiv - Microbiology 2020Quote: ... Cell suspensions were clarified by centrifugation (20 000 x g, 10 minutes, 4°C) and total soluble protein quantified58 using a kit (Bio-Rad Protein Assay Kit) and BSA as a standard.
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bioRxiv - Cell Biology 2020Quote: ... Lysates were clarified for 5 min at 10,000 × g at +4□C and protein concentration was determined using DC Protein Assay (Bio-Rad Laboratories, Hercules, CA, USA). For immunoprecipitation ...
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bioRxiv - Biochemistry 2019Quote: ... The microsomal fraction was aliquoted and stored at −80 °C after protein quantification with the Quick Start Bradford Protein Assay Kit (Bio-Rad Laboratories, Munich, Germany).
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bioRxiv - Neuroscience 2023Quote: ... and denaturated by β-mercaptoethanol at 75°C before loading 20-30 µg protein onto 4-20% Mini-PROTEAN TGX Stain-FreeTM Protein Gels (Bio-Rad, Hercules, CA, USA) and electrophoresed at 120 V for 120 min ...
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bioRxiv - Neuroscience 2023Quote: ... and denaturated by β-mercaptoethanol at 75°C before loading 50-60 µg protein onto 4-20% Mini-PROTEAN TGX Stain-FreeTM Protein Gels (Bio-Rad, Hercules, CA, USA) and electrophoresed at 120 V for 120 min ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were incubated at 60°C for 15 min in 4x Laemmli sample buffer (Bio-Rad). 20–40µg liver lysate protein was separated by SDS-PAGE and transferred to a PVDF membrane ...
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bioRxiv - Immunology 2022Quote: ... 25μg of protein were denatured at 95°C for 5min in Laemmli Sample Buffer (BIORAD #1610747) with DTT 0.1M ...
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bioRxiv - Immunology 2022Quote: ... the proteins were incubated 5 minutes at 95°C in Laemmli sample buffer (Bio-Rad laboratories), with or without addition of β-mercaptoethanol (Sigma-Aldrich Chemie) ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 μg protein samples were heated at 95 °C in 4x Laemmli sample buffer (Bio-Rad) for 5 min Samples were run down a Novex 4–12% Tris-glycine gel (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... at 100 °C for 10 minutes and then loaded onto precast protein gels (Bio-Rad, #4561084). The gels were transferred onto PVDF membranes using iBlot PVDF gel transfer (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2019Quote: ... with a Carbo-P precolumn (Bio-Rad, USA) and an inline deashing system (Bio-Rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... gel filtration bead (P-200 beads from BioRad) was soaked in water O/N at room temp ...
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bioRxiv - Biophysics 2023Quote: ... Bands (P) were integrated using ImageLab software (BioRad) and divided by the total RNA per lane (T ...
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bioRxiv - Pathology 2019Quote: ... Protein concentration in thawed samples (stored at –80°C) was measured using the Bradford method (Bio-Rad).
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bioRxiv - Neuroscience 2020Quote: ... 20 to 40μg of protein were boiled at 95°C for 5 minutes in Laemmli buffer (Biorad), separated on a SDS-PAGE gel and then electrotransfered (100V ...
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bioRxiv - Neuroscience 2019Quote: ... The blotting of the proteins was done at 4 °C overnight at 40 V (PowerPac, Bio-Rad).
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bioRxiv - Neuroscience 2021Quote: ... Aliquots of protein were boiled at 95°C in 2X Lamelli buffer (Bio-Rad, Hercules, CA, USA) for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... All steps were done at 4 °C and concentrations of all proteins were determined by Bio-Rad Protein Assay Dye Reagent Concentrate.
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bioRxiv - Cancer Biology 2023Quote: ... Bound proteins were then eluted through boiling at 100°C in Laemmli sample buffer (BIORAD, CA, USA). The bound proteins were eluted at 37°C for 10 min.
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bioRxiv - Immunology 2024Quote: ... 25µg of protein were denatured at 95°C for 5min in Laemmli Sample Buffer (161747, Bio-Rad) with DTT 0.1M ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell debris was pelleted by microcentrifugation at 4°C and protein levels quantified using Bradford Assays (BioRad) as previously described (38) ...
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bioRxiv - Synthetic Biology 2019Quote: ... Protein estimations were performed using the Bio-Rad microtiter Bradford protein assay (California, USA) according to the instructions provided (Bio-Rad version: Lit 33 Rev C) and measurements were made at 595 nm using a SpectraMax Plus ...
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bioRxiv - Molecular Biology 2021Quote: ... and purified on P-30 spin columns (Bio-Rad). Chemically synthesized oligoribonucleotides A27 and U18 were kind gifts of Prof ...
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bioRxiv - Plant Biology 2020Quote: ... RNase-free Bio-Spin columns P-30 (Bio-Rad) were used for radiolabeled RNA ...
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bioRxiv - Genomics 2024Quote: ... buffer exchange using P-30 columns (Bio-Rad Laboratories) was performed ...
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bioRxiv - Neuroscience 2021Quote: ... 10-15 μg of protein was boiled at 95°C for 5 min in Laemmli buffer (Bio-Rad), separated on a 4–12 % SDS/PAGE gel and then transferred using the Transblot®-Turbo™ Transfer system (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates of equal total protein concentration were heated at 90°C in 1 × loading buffer (Bio-Rad 1610747) for ten minutes ...
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bioRxiv - Synthetic Biology 2021Quote: ... After 30 min centrifugation at 4 °C protein concentration of the lysate was determined by Bradford assay (Biorad). In parallel ...
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bioRxiv - Genomics 2023Quote: ... proteins were transferred (80 V; 2 h; 4°C) onto a 0.2 µm nitrocellulose membrane (Bio-Rad 1620112). Membranes were air-dried ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Proteins were denatured for 5 min at 95 °C and cooled to RT before acrylamide (#1610140, Bio-Rad) was added to a final concentration of 4% (w/v ...
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bioRxiv - Biochemistry 2023Quote: ... Protein samples were boiled for 5 minutes at 95°C in 4x Laemmli Sample Buffer (Bio-Rad, 1610747) and run on SDS-acrylamide gels ...
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bioRxiv - Cell Biology 2022Quote: ... Precision Plus Protein™ Western C™ Blotting standards and Precision Protein™ Streptactin-HRP conjugate were used to estimate molecular weight (BioRad, cat# 1610380 and cat#1610376). Relative quantification was performed using ImageJ software (version 1.53p (22) ...
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bioRxiv - Microbiology 2020Quote: ... 7.5 mg of proteins were diluted in ChIP buffer supplemented with 0.01% SDS and precleared 1 h at 4 °C with 50 μl of protein A agarose beads (BioRad) and 100 μg BSA ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were heated for 5’ at 95°C in Lämmli buffer and protein lysates were resolved on polyacrylamide Mini-PROTEAN TGX gels (BioRad) and transferred onto nitrocellulose membrane by wet-transfer ...
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bioRxiv - Neuroscience 2022Quote: ... Lysates were then centrifuged at 12,000 rpm at 4°C for 20min and total protein extracts were boiled in Laemmli sample buffer (BioRad), separated by SDS-PAGE under reducing conditions and transferred to PVDF membranes (Millipore) ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was incubated at 4°C for 1 h with Bio-Beads™ SM-2 resin (Bio-Rad) equilibrated with 100 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... The immunoprecipitated proteins were eluted by heating at 100°C during 15 minutes in 4x Laemmli Sample Buffer (BIORAD). Immunoprecipitated protein complexes were subjected to Western blot analysis ...
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bioRxiv - Biochemistry 2021Quote: ... The enriched protein were eluted by heating at 95°C for 30 minutes in 1X Laemmli sample buffer (BioRad) and processed to western blot ...
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bioRxiv - Neuroscience 2020Quote: ... Lysates were cleared of debris by centrifugation at 15,000 x g for 15 min at 4°C and then normalized for protein content using Bradford assay (BioRad). Normalized protein lysates were fractionated by 10 % SDS/PAGE and transferred onto a PVDF membrane (GE Healthcare Life Sciences ...
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bioRxiv - Plant Biology 2022Quote: ... 4 °C) and the supernatant was taken up and the protein concentration was determined using the Bradford assay (Biorad).
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bioRxiv - Neuroscience 2023Quote: ... were centrifuged at 20,000 x g for 25 min at 4⁰C and supernatants were assayed for total protein (Bradford assay, BioRad). Aliquots of WCL (30 µg total protein ...
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bioRxiv - Neuroscience 2023Quote: ... Protein extracts were denatured 5 min at 95°C in a Laemmli buffer (Bio-Rad, Marnes-la-Coquette, France) with β-mercaptoethanol (1/10 ...