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Citations for Bio-Rad :
1 - 50 of 8780 citations for Anti Flag Affinity Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: 100 µL of well mixed anti-FLAG M2 Affinity Gel aliquots were loaded on columns (Micro Bio-Spin Columns, Bio-Rad) and washed two times with 1 mL of cell lysis buffer by gravity flow ...
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bioRxiv - Cell Biology 2022Quote: ... The anti-FLAG immunoprecipitates were subjected to gel electrophoresis and visualized by Oriole fluorescent gel staining (BioRad). Specific bands were excised and digested in gels with trypsin ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and affinity purified using Affi-gel 10 (Bio-Rad, 153-6099). Commercial available antibodies are mouse anti-Flag (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Antibodies against CBX2 were affinity purified using the Affi-gel (Bio-Rad, Affi-Gel 10, Cat# 1536099)-coupled FLAG-CBX2-ΔCBox ...
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bioRxiv - Genetics 2023Quote: ... The rabbit polyclonal antiserum was raised against purified recombinant Thp1 protein and subsequently affinity purified as Thp1p coated affinity beads (Affi-Gel 10 beads, Bio-Rad) according to standard procedures ...
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bioRxiv - Microbiology 2022Quote: ... Anti-FLAG clone 1/27 was coupled to Affi-Gel 10 resin following the manufacturer’s instructions (Bio-Rad). A549 cells were inoculated at an MOI of 0.2 with S009 PB2-FLAG or S009 PB2-627K-FLAG in a 10 cm dish ...
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bioRxiv - Plant Biology 2019Quote: Affinity purified proteins were separated by SDS-PAGE using Mini-PROTEAN TGX gels (BioRad) and stained with the Novex colloidal blue staining kit (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: Anti-Flag antibody precipitated proteins in the concentrated eluate were resolved in a 4-15% Criterion TGX gel (BioRad) and stained by Colloidal Blue dye (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... Anti–UNC-45 was affinity-purified using Affigel (BioRad)-conjugated to the MBP fusion ...
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bioRxiv - Neuroscience 2022Quote: ... Antisera to GST-MTD was affinity purified by pre-absorption on an Affi-Gel (Bio-Rad) column coupled with GST ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The DMC1 antibody was then purified from antisera following affinity purification (using Affi-Gel 15 from Biorad) protocol ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins immunoprecipitated with anti-FLAG antibody were separated by SDS-PAGE (4-15% Mini-PROTEAN®TGX™ Precast Protein Gels (Bio-RAD)) ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies were affinity-purified over the corresponding GST-LRRCC1 fusion bound to Affi-Gel 10 resin (Bio-Rad). Other primary and secondary antibodies used in this study are listed in Table 1.
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bioRxiv - Biophysics 2023Quote: ... NICD-FLAG-containing lysates were separated on a 7.5% acrylamide Mini-PROTEAN TGX gels (Bio-Rad) and transferred to PVDF membranes using a Trans-Blot Turbo semi-dry transfer system (Bio-Rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... The labeled GyrA14-I491C was loaded onto a pre-equilibrated CcdB affinity column prepared using Affi-gel 10 (Bio-Rad), and incubated at 4°C for 4 hours on a rotary mixer to enable binding ...
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bioRxiv - Molecular Biology 2022Quote: ... His-tagged recombination proteins were purified with cobalt-chelating affinity chromatography (Pierce) and desalted with HEPES buffer (pH 7.15) using the gel filtration column (Bio-Rad).
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bioRxiv - Biochemistry 2021Quote: Primary screening for nanobody binding was achieved by a small-scale pulldown method in which Affi-gel 10 affinity resin (Bio-Rad) was bound to laforin and subsequently to each purified VHH clone34 ...
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bioRxiv - Biochemistry 2022Quote: ... FLAG elutions were analyzed by SDS-PAGE on 4-20% Mini- PROTEAN TGX Precast Protein Gels (Bio-Rad) without prior heating to preserve the GFP fluorescence ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was filtered through a 0.45-μm syringe filters and solubilized NFL was then purified from the supernatant by affinity chromatography using Bio-gel® HT hydroxyapatite (130-0150, Bio-Rad) and following the protocol of Leung and Liem [37] ...
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bioRxiv - Molecular Biology 2024Quote: Nuclear lysates of inputs and affinity-purified elution were loaded onto 4-15 % Criterion TGX Stain-Free precast polyacrylamide gels (BIO-RAD, Cat# 5678085) and transferred to nitrocellulose membranes which were subsequently blocked by 5% BSA for 1Chour at RT ...
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bioRxiv - Biochemistry 2021Quote: ... Ni-NTA and FLAG elutions were analyzed by SDS-PAGE on AnykD Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad). The samples were not heated before loading to preserve the fluorescence of the GFP and mCherry tags ...
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bioRxiv - Developmental Biology 2021Quote: After affinity purification anti-GFP beads were resuspended in 15 μl of 4× Laemmli sample buffer (Biorad), boiled at 99 °C for 10 min and supernatants were loaded on 4–12% Criterion XT Bis–Tris precast gel (Biorad) ...
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bioRxiv - Developmental Biology 2019Quote: ... pH 8.0) prior to affinity purification by immobilized metal ion affinity chromatography (IMAC) using Chelex 100 Resin (Bio-Rad). Purified protein in PBS was mixed 1:1 with Freund’s complete adjuvant for immunization or with Freund’s incomplete adjuvant for booster injections ...
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bioRxiv - Molecular Biology 2022Quote: ... 30 μg protein of whole cell lysate or variable volume of Flag-IP samples was loaded into 4-20% Mini-PROTEAN® TGX Stain-Free(tm) Gels (Bio-Rad) and then transferred onto a PVDF membrane using the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... the soluble fraction containing recombinant MBP-RON11-His6 protein was affinity purified twice on immobilized-metal affinity chromatography (IMAC) column using FPLC (Biorad) and further purified on CHT Ceramic Hydroxyapatite column ...
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bioRxiv - Immunology 2024Quote: ... rabbit anti-FLAG-HRP (clone D6W5B, CellSignalingTechnology #86861S, RRID:AB_2800094) or anti-Strep (clone Strep-tag II StrepMAB-Classic, Biorad #MCA2489P, RRID:AB_609796) detection Abs were added for 30 minutes ...
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bioRxiv - Developmental Biology 2020Quote: Streptavidin and anti-GFP beads after affinity purification were resuspended in 15 μl of 4× Laemmli sample buffer (Biorad), boiled at 99 °C for 10 min and supernatants were loaded on 4–12% Criterion XT Bis–Tris precast gel (Biorad) ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were blocked using 5% BSA in 1X TBST and incubated overnight with primary antibody (anti-Cas9; 1:1000 dilution, Diagenode #C15200216, Anti-FLAG; 1:2000 dilution, Sigma-Aldrich #F1804, anti-β-Tubulin; 1:1000 dilution, Bio-Rad #12004166). Then membranes were washed with 1X TBST 3 times (10mins each wash ...
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bioRxiv - Microbiology 2021Quote: ... anti-FLAG (Cell Signalling Technologies, 2908S), anti-SARS-CoV-2-ORF8 (MRCPPU, University of Dundee, DA088, [37]) and anti-Tubulin (BioRad, MCA77G). Donkey anti-rabbit IRDye 800 and goat anti-rat IRDye 680 (Li-cor Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... membranes were subject to sequential incubation with anti-FLAG mouse antibody (1: 2000 dilution) and secondary anti-mouse IgG conjugated to HRP (Bio-Rad). After washing three times with TBST buffer ...
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bioRxiv - Immunology 2022Quote: ... prior to overnight incubation in the same buffer with 1:2000 anti-FLAG-HRP (clone D6W5B, CellSignallingTechnology #86861S) or anti-Strep (clone Strep-tag II StrepMAB-Classic, Biorad #MCA2489P) or goat anti-mouse Igκ-HRP (SouthernBiotech #1050-05) ...
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bioRxiv - Cell Biology 2022Quote: ... and affinity purified anti-HSP-90 antibodies by use of an MBP-HSP-90 antigen coupled to Affigel matrix (BioRad), using a procedure previously published (Benian et al. ...
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bioRxiv - Biophysics 2023Quote: ... gels were imaged using Gel Doc EZ gel system (Bio-Rad). Only samples that contained <5% free DNA were used for measurements.
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bioRxiv - Cell Biology 2024Quote: ... Stained gels were imaged with Gel Doc XR+ Gel Documentation System (Biorad).
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bioRxiv - Pathology 2019Quote: ... Affi-gel 10 affinity resins were covalently cross-linked to his-tagged GT198 protein as antigen for affinity purification of anti-GT198 according to the manufacturer’s protocol (Bio-Rad, Hercules, CA). FFPE tumor sections or tumor microarrays were deparaffinized and dehydrated through xylene and ethanol series ...
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bioRxiv - Developmental Biology 2023Quote: Affi-Gel Blue Gel beads (Bio-Rad) were incubated with 1 mg/ml Fgf2 (recombinant human basic Fgf ...
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bioRxiv - Molecular Biology 2020Quote: ... FLAG (EGFR) signals were quantified for the FLAG immunoprecipitation using Image Lab (BioRad Laboritories, version 6.0 build 26). Lanes and bands were manually defined using the volume tool ...
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bioRxiv - Neuroscience 2019Quote: ... to which rabbit anti-PGRN antibody-conjugated Affi-Gel 15 (Bio-Rad Laboratories) or GFP-Trap beads were added ...
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bioRxiv - Bioengineering 2023Quote: ... Gel image was taken by Gel Doc XR+ Gel Documentation System (Bio-Rad).
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bioRxiv - Biochemistry 2021Quote: ... All gels were then dried using a gel drier (Bio-Rad gel dryer 583) and the radiolabelled acyl-ACP was detected by using the phosphorimager (Amersham Typhoon FLA 9000).
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bioRxiv - Cell Biology 2023Quote: ... Gel imaging was performed using the Gel Doc XR+ Gel Documentation System (BioRad, USA). To verify mtDNA cutting at the target site ...
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bioRxiv - Genomics 2020Quote: ... Gels were imaged with Gel Doc (Bio-Rad) using Alexa 488 fluorescence settings ...
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bioRxiv - Biochemistry 2022Quote: ... Gels were dried with a gel dryer (Biorad) and bands were detected using an FLA-5000 phosphoimager (Fuji Film).
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bioRxiv - Developmental Biology 2022Quote: Affi-Gel Blue Gel Beads (Bio-Rad # 1537302) with blastocyst size were incubated with TNFα (0.1% BSA/PBS (410-MT-010 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... gels were subsequently imaged using a Gel Doc EZ Gel Documentation System (Bio-Rad, UK).
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bioRxiv - Neuroscience 2022Quote: ... Images of gels were captured by molecular gel doc (BIO-RAD GEL/CHEMI DOC, Japan).
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bioRxiv - Biochemistry 2022Quote: ... Proteins were purified by Nickel charged Immobilized Metal Affinity Chromatography (BioRad, CA). The affinity tags were removed during overnight incubation with SENP (made in-house) ...
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bioRxiv - Biochemistry 2021Quote: ... imaged using Gel Doc EZ gel documentation system (BioRad), and analyzed to determine the fractions containing both DnaB and ssDNA ...
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bioRxiv - Biochemistry 2022Quote: ... and purified on Affi-Gel Blue Gel (Bio-Rad). Hsp104 was eluted in elution buffer (50 mM Tris-HCl pH = 8.0 ...
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bioRxiv - Molecular Biology 2019Quote: ... gel purified on a 5% polyacrylamide TBE gel (BioRad) and sequenced on an Illumina HiSeq (Edinburgh Genomics) ...