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Citations for Bio-Rad :
1 - 50 of 5656 citations for Anti FLAG M5 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... ELISAs were performed with the Bio-Rad TeSeE Short Assay Protocol (SAP) Combo Kit (BioRad Laboratories Inc., Hercules, CA, USA). Importantly ...
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bioRxiv - Molecular Biology 2022Quote: ... and their CWD status was independently identified utilizing the Bio-Rad TeSeE Short Assay Protocol (SAP) Combo Kit (BioRad Laboratories Inc., Hercules, CA, USA). Positive RPLNs were confirmed by IHC at the Colorado State University Veterinary Diagnostic Laboratory) ...
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bioRxiv - Immunology 2024Quote: ... rabbit anti-FLAG-HRP (clone D6W5B, CellSignalingTechnology #86861S, RRID:AB_2800094) or anti-Strep (clone Strep-tag II StrepMAB-Classic, Biorad #MCA2489P, RRID:AB_609796) detection Abs were added for 30 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were blocked using 5% BSA in 1X TBST and incubated overnight with primary antibody (anti-Cas9; 1:1000 dilution, Diagenode #C15200216, Anti-FLAG; 1:2000 dilution, Sigma-Aldrich #F1804, anti-β-Tubulin; 1:1000 dilution, Bio-Rad #12004166). Then membranes were washed with 1X TBST 3 times (10mins each wash ...
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bioRxiv - Microbiology 2021Quote: ... anti-FLAG (Cell Signalling Technologies, 2908S), anti-SARS-CoV-2-ORF8 (MRCPPU, University of Dundee, DA088, [37]) and anti-Tubulin (BioRad, MCA77G). Donkey anti-rabbit IRDye 800 and goat anti-rat IRDye 680 (Li-cor Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... membranes were subject to sequential incubation with anti-FLAG mouse antibody (1: 2000 dilution) and secondary anti-mouse IgG conjugated to HRP (Bio-Rad). After washing three times with TBST buffer ...
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bioRxiv - Immunology 2022Quote: ... prior to overnight incubation in the same buffer with 1:2000 anti-FLAG-HRP (clone D6W5B, CellSignallingTechnology #86861S) or anti-Strep (clone Strep-tag II StrepMAB-Classic, Biorad #MCA2489P) or goat anti-mouse Igκ-HRP (SouthernBiotech #1050-05) ...
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bioRxiv - Cell Biology 2022Quote: ... The anti-FLAG immunoprecipitates were subjected to gel electrophoresis and visualized by Oriole fluorescent gel staining (BioRad). Specific bands were excised and digested in gels with trypsin ...
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bioRxiv - Microbiology 2022Quote: ... Anti-FLAG clone 1/27 was coupled to Affi-Gel 10 resin following the manufacturer’s instructions (Bio-Rad). A549 cells were inoculated at an MOI of 0.2 with S009 PB2-FLAG or S009 PB2-627K-FLAG in a 10 cm dish ...
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bioRxiv - Molecular Biology 2020Quote: ... FLAG (EGFR) signals were quantified for the FLAG immunoprecipitation using Image Lab (BioRad Laboritories, version 6.0 build 26). Lanes and bands were manually defined using the volume tool ...
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bioRxiv - Cell Biology 2024Quote: Anti-Flag antibody precipitated proteins in the concentrated eluate were resolved in a 4-15% Criterion TGX gel (BioRad) and stained by Colloidal Blue dye (Invitrogen) ...
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bioRxiv - Molecular Biology 2019Quote: ... Membranes were incubated with HRP-conjugated secondary antibodies for both FLAG and GFP stainings (Rabbit anti-goat-HRP conjugate, #1721034, Bio-Rad) diluted at 1:5000 ...
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bioRxiv - Microbiology 2020Quote: 100 µL of well mixed anti-FLAG M2 Affinity Gel aliquots were loaded on columns (Micro Bio-Spin Columns, Bio-Rad) and washed two times with 1 mL of cell lysis buffer by gravity flow ...
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bioRxiv - Microbiology 2019Quote: ... and FLAG-cMCF-HA recovered as described above were prepped using the Bio-Rad ReadyPrep 2-D Cleanup Kit (Bio-Rad 1632130). The sample was then loaded onto 11 cm pH 3-10 immobilized pH gradient (IPG ...
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bioRxiv - Developmental Biology 2021Quote: ... by immunoprecipitation of endogenous or endogenously Flag-tagged Piwi (eF-Piwi): The anti-Piwi antibody and Surebeads Protein A Magnetic bead (Bio-Rad, 1614013) were used to immunoprecipitated endogenous Piwi-piRNAs from witld type OSC ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins immunoprecipitated with anti-FLAG antibody were separated by SDS-PAGE (4-15% Mini-PROTEAN®TGX™ Precast Protein Gels (Bio-RAD)) ...
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bioRxiv - Microbiology 2020Quote: ... HRP-conjugated anti-rabbit and anti-mouse were used for detection using the chemiluminescence detection kit Clarity ECL (Biorad).
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bioRxiv - Cancer Biology 2020Quote: Fifteen micrograms pGuide-HCFC1-N and 10 μg pCRIS-mCherry-FLAG-dTAG-HCFC1 were electroporated (BioRad, Hercules ...
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bioRxiv - Biophysics 2023Quote: ... NICD-FLAG-containing lysates were separated on a 7.5% acrylamide Mini-PROTEAN TGX gels (Bio-Rad) and transferred to PVDF membranes using a Trans-Blot Turbo semi-dry transfer system (Bio-Rad) ...
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bioRxiv - Physiology 2020Quote: ... Bradford protein assay kit as well as anti-mouse and anti-rabbit secondary antibodies were obtained from Bio-Rad (Hercules, CA). Antibodies against ERα (F-10 mouse – for WB) ...
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bioRxiv - Bioengineering 2020Quote: ... anti-CD63 and anti-CD81 detection antibodies (supplied in the MACSPlex kit, 5 µl each) or anti-decoy receptor antibodies (AlexaFluor647-labelled anti-human TNFR1, Bio-Rad, cat #MCA1340A647 ...
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bioRxiv - Cell Biology 2022Quote: ... the proteins were eluted from the FLAG beads with 60 μL 2x Laemmli buffer (Bio-Rad, 1610747) supplemented with 10 mM DTT and incubated at 95°C for 10 min ...
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bioRxiv - Biochemistry 2022Quote: ... FLAG elutions were analyzed by SDS-PAGE on 4-20% Mini- PROTEAN TGX Precast Protein Gels (Bio-Rad) without prior heating to preserve the GFP fluorescence ...
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bioRxiv - Biochemistry 2023Quote: ... Clear lysate was passed onto M1-FLAG resin pre-packed into glass Econo columns (Biorad, Cat. no. 7372512) and allowed to gravity-flow at 1-2mL min-1 ...
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bioRxiv - Microbiology 2023Quote: ... anti-CD45-APC (clone UM16-6, LYNX Rapid APC Antibody Conjugation Kit, both Bio-rad) and thrombocyte marker K1-PE (LYNX Rapid RPE Antibody Conjugation Kit ...
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bioRxiv - Microbiology 2022Quote: ... the samples were washed extensively with lysis buffer and the proteins were eluted by boiling 5 ul of the resin with 5x SDS loading buffer and the proteins were detected by western blot analysis using rabbit antibody to FLAG (1:2,000, BioRad) or mouse antibody to Myc (1:2000 ...
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bioRxiv - Physiology 2019Quote: ... ACPA was detected using the BioPlex® 2200 System anti-CCP reagent kit (Bio-Rad, US) and cutoff levels used were according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... After loading the lysate into the packed volume of 100 µL of FLAG agarose on a gravity column (Bio-Rad) and reloading the flow through twice ...
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bioRxiv - Biochemistry 2021Quote: ... Ni-NTA and FLAG elutions were analyzed by SDS-PAGE on AnykD Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad). The samples were not heated before loading to preserve the fluorescence of the GFP and mCherry tags ...
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bioRxiv - Microbiology 2023Quote: ... anti-CD8-PerCP-Cy5.5 (clone CT8, Southern Biotech, LYNX Rapid PerCP Antibody Conjugation Kit, Bio-rad, Feldkirchen, Germany), anti-CD45-APC (clone UM16-6 ...
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bioRxiv - Microbiology 2019Quote: ... and visualized with rabbit-α-FLAG (1:3,000) primary and goat-α-rabbit-HRP conjugated secondary antibodies on the ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Molecular Biology 2022Quote: ... 30 μg protein of whole cell lysate or variable volume of Flag-IP samples was loaded into 4-20% Mini-PROTEAN® TGX Stain-Free(tm) Gels (Bio-Rad) and then transferred onto a PVDF membrane using the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... and HopAM1 and C-terminal Flag-tagged IpaH4 were generated by PCR amplification using primers containing Flag sequences and flanking SacII / PacI off pIB/V5-His templates using iProof DNA polymerase (Bio-Rad; Table S4) and cloned into the pDGOpIE2 vector ...
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bioRxiv - Microbiology 2022Quote: ... a 1:1000 dilution of α-FLAG M2 monoclonal antibodies and/or 1:10,000 dilution of HRP-labeled α-rabbit secondary antibodies (BioRAD Catalog #170-6515) on nitrocellulose membranes ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti–PAI-1 antibody was coupled to carboxylated polystyrene beads (Luminex) using a Bio-Plex amine coupling kit (Bio-Rad). The coupled beads were then incubated with samples and bound PAI-1/tPA complexes detected using biotinylated rabbit anti-tPA antibody (Molecular Innovations ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-conjugated secondary anti-mouse antibody (Amersham; NA931S) and anti-rabbit antibody (Amersham; NA934VS) at 1:10,000 and Clarity western ECL substrate detection kit (Bio-Rad; 170-5060) were used to detect the signals ...
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bioRxiv - Cell Biology 2020Quote: ... and goat anti-rabbit or anti-mouse (Bio-Rad) conjugated with horseradish peroxidase (HRP ...
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bioRxiv - Cancer Biology 2019Quote: ... Secondary antibodies—anti-mouse or anti-rabbit-HRP (Bio-Rad)—were diluted at 1:5,000 ...
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bioRxiv - Cell Biology 2023Quote: ... goat anti-mouse or goat anti-rabbit StarBrightBlue700 (Bio-Rad), and hFAB Actin Rhodamine primary antibody (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... and secondary anti-goat anti-rabbit HRP conjugated antibodies (Bio-Rad). For experiments assessing specificity of murine sera and vaginal washes ...
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bioRxiv - Bioengineering 2020Quote: ... anti-infliximab (HCA213G) and anti-adalimumab (HCA205) were ordered from BioRad. Similarly ...
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bioRxiv - Cell Biology 2020Quote: ... Horseradish peroxidase-linked anti-mouse or anti-rabbit antibodies (Bio-Rad) were used at 1:7,000 ...
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bioRxiv - Immunology 2024Quote: ... Anti-F4/80 (rat anti-mouse MCA497A647, Biorad, dilution 1:500) and anti-Parp14 (sc377150 ...
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bioRxiv - Immunology 2021Quote: ... cytokine kit (Bio-Rad). LDH release was quantified using an LDH Cytotoxicity Assay Kit (Thermo scientific ...
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bioRxiv - Immunology 2020Quote: ... cytokine kit (Bio-Rad) following the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... Western blots were detected using chemiluminescence detection kit (Pierce) or chemiluminescence kit (BioRad).
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bioRxiv - Cell Biology 2023Quote: ... kits and cDNA was generated using iScript cDNA Synthesis kits (Bio-Rad #1708890) as per the manufacturer’s specifications ...
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bioRxiv - Microbiology 2023Quote: ... a reverse transcription kit (iScript select cDNA synthesis kit, Bio-Rad, Hercules, CA) was used to create cDNA from the total RNA with gene-specific antisense primers (Table S1) ...
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bioRxiv - Physiology 2019Quote: ... RNA was isolated and cDNA was formed using commercial kits according to manufacturer’s instructions (NucleoSpin miRNAs kit; Macherey-Nagel, Düren, Germany; iScript cDNA synthesis kit, Bio-Rad, Veenendaal, Netherlands). Quantity and chemical purity of RNA and cDNA were assessed using Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... HRP-conjugated anti-rabbit IgG and HRP-conjugated anti-mouse IgG (BioRad). For mass spectrometry analysis ...