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Citations for Bio-Rad :
201 - 250 of 277 citations for 8 Fluoroisoquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... 10nM of templates with each promoter type were added to TXTL reactions and incubated at 30 °C for 8 hours (T100 Thermal Cycler, Bio-Rad). Post-incubation ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed in Native NiNTA lysis buffer (50mM NaH2PO4, 300mM NaCl, pH 8, 10mM imidazole) and protein concentration was measured via Bradford assay (Bio-Rad). 500-800μg of protein were incubated with NiNTA beads at 4°C overnight ...
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bioRxiv - Genetics 2021Quote: ... or 1:8 (for all other quantifications) and 2ul was used to quantify gene expression using iTaq Universal SYBR Green Supermix (Bio-Rad) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... cell lysates were separated by 8% SDS-PAGE and Western blotted onto a PVDF membrane (semidry transfer, 15 V, 45 min, Trans blot Bio-Rad). The membranes were incubated overnight at 4°C in blocking solution 5% (w/v ...
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bioRxiv - Neuroscience 2021Quote: ... Thirty micrograms of total protein from each sample were loaded on 8% SDS-PAGE and transferred to PVDF membranes (Bio-Rad).
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bioRxiv - Immunology 2021Quote: ... Cell lysates were separated on 8-12% SDS-PAGE gel and transferred onto a PVDF membrane (Bio-Rad, Hercules, CA, USA). The membrane was incubated overnight with the respective primary antibodies at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... 50 μl of eluted samples were electrophoresed for 8 min at 200 V on a 10% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and protein was stained using Quick Blue Stain ...
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bioRxiv - Neuroscience 2022Quote: ... The pellet was resuspended in 50 μL 8M urea buffer (8 M urea, 100 mm NaHPO4, pH 8.5) and boiled in Laemmli sample buffer (BioRad, 161-0737) at 98°C for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... and treated with or without heat denaturation (96°C, 8 min) and ran on a TGX stain-free precast gel (Bio-Rad) for 38 min at 200 V ...
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bioRxiv - Neuroscience 2022Quote: ... and 500 ng RNA (RIN>8) was transcribed to cDNA using the iScript first strand cDNA synthesis kit (Bio-Rad 1708891). qPCR was carried out on a 384-well Quantstudio Flex system (ThermoFisher ...
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bioRxiv - Immunology 2022Quote: ... Equal volumes of diluted (1:8) cDNA were loaded for qPCR analysis along with the SsoAdvanced Universal SYBR Green Supermix (BioRad #1725272) and 0.5 μM forward and reverse primers for target genes (listed below) ...
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bioRxiv - Microbiology 2023Quote: ... Blots were washed 8 × 5 minutes in TBST and then ladder lanes were reacted with Precision Plus Strep Tactin HRP Conjugate (BioRad 1610380) diluted 1:10,000 in TBST ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by isoelectric focusing on immobilized linear pH gradient strips (pH 5 to 8, Bio-Rad, Hercules, CA, USA) before separation by SDS-PAGE ...
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bioRxiv - Cancer Biology 2023Quote: ... and then the gel was washed in destain buffer (8% acetic acid) until the background was washed off for visualization by ChemiDoc Imaging system (Bio-Rad).
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bioRxiv - Immunology 2023Quote: ... 8 µg protein per condition were separated by SDS-PAGE and blotted onto a 0.2 µm nitrocellulose membrane (Bio-Rad, #1704158) using a Trans-Blot® Turbo™ Transfer System (Bio-Rad) ...
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bioRxiv - Physiology 2023Quote: ... The protein extract (40μg) was separated on 8 or 10% polyacrylamide mini gel and transferred to a PVDF membrane (Bio-Rad, USA). Blotted membranes were incubated overnight at 4°C in SuperBlock(PBS ...
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bioRxiv - Bioengineering 2023Quote: We used conjugated magnetic beads (6.5 μm) (Bio-Plex Pro Human Chemokine IL-8 / CXCL8 Set #171BK31MR2, BioRad, Hercules, CA, USA) for the binding assay ...
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bioRxiv - Neuroscience 2022Quote: ... and then subjected to SDS polyacrylamide gel electrophoresis (PAGE) with 8% polyacrylamide and transferred to polyvinylidene difluoride (PVDF) membrane (BioRad Laboratories). The membranes were blocked for 1 h in Tris buffered saline with Tween 20 (TBST ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were boiled for 8–10 min at 95°C and loaded onto precast 12% Bis-Tris gels (Bio-Rad) with MES SDS running buffer (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: ... were separated by 8% acrylamide SDS-PAGE and transferred to a nitrocellulose membrane using the Trans-Blot Turbo transfer kit (Bio-Rad). 5% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... Aliquots of cell lysates (40-60 μg of protein) were subjected to SDS/PAGE on an 8 to 15% (vol/vol) acrylamide gel (MiniProtean; Bio-Rad) including PageRuler Prestained Protein Ladder (Thermo) ...
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bioRxiv - Immunology 2023Quote: ... Even amounts of each protein sample were separated on a 8–10% polyacrylamide gel using Mini-PROTEAN Tetra Cell electrophoresis system (Bio-Rad). Upon completion of electrophoresis ...
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bioRxiv - Microbiology 2023Quote: ... Parasites were incubated with drug for 8 or 12 h (short or long assays, respectively) and stained with 5 μg/ml ethidium bromide (EtBr, Bio-Rad) for 10 min followed by immediate flow-cytometry analysis (Becton Dickinson ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were heated at 95°C for 5 minutes and cooled to room temperature before being separated on an 8% tris-glycine SDS-PAGE gel and transferred to a nitrocellulose membrane using a semi-dry transfer machine (Bio-Rad). Membranes were blocked in 5% skim milk dissolved in Tris-buffered saline with 0.1% Tween-20 (TBST ...
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bioRxiv - Neuroscience 2023Quote: ... The pellet was resuspended in 8M urea buffer (8 M urea, 100 mm NaHPO4, pH 8.5) and boiled in Laemmli sample buffer (BioRad, 161-0737) at 98°C for 5 min ...
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bioRxiv - Immunology 2024Quote: ... Equal amounts of pre-cleared lysate samples were separated in 8 – 16% Mini protean-TGX stain free protein gel (Bio-Rad) and the gel was transferred to a Trans-Blot Turbo Mini-PVDF membrane (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... Approximately 20-30 μg of protein was resolved on 8% polyacrylamide gels and transferred onto nitrocellulose membranes using semi-dry Trans-Blot TurboTM (Bio-Rad). Following antibody incubation (anti-GFP ...
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bioRxiv - Plant Biology 2023Quote: ... The total proteins were separated by 8%-12% SDS-PAGE gels and blotted onto an Immune-Blot polyvinylidene difluoride (PVDF) membrane (Bio-Rad). All the antibodies were obtained from commercial suppliers (anti-GFP and anti-MYC antibodies from Roche ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein (20-30 μg) were resolved by 8 to 15% SDS PAGE MiniPROTEAN® TGX gels (Bio-Rad), from samples previously prepared with Laemmli Buffer 4X™ (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... carried out in glass tubes filled with polyacrylamide gel using a mixture of ampholytes 3/10 and 5/8 (Bio-Rad, USA) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... Electrofocusing was performed in glass capillaries with polyacrylamide gel using a mixture of ampholytes 3/10 and 5/8 (#163-1112, #163-1192, Bio-Rad, USA). The second direction is standard SDS 5-10% PAGE followed by staining with Coomassie Brilliant Blue G-250 (#31-4-58-1 ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... Proteins were separated on SDS-containing 8 to 12 % polyacrylamide gels and transferred to PVDF membranes (Bio-Rad Laboratories, Inc., Hercules, USA). After staining with primary and secondary antibodies ...
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bioRxiv - Bioengineering 2020Quote: ... Samples were denatured by incubating at 95°C for 10 minutes and loaded onto a denaturing 10% polyacrylamide gel with 8 M Urea in a Mini-PROTEAN® Electrophoresis System (BIO-RAD). Electrophoresis was performed at 240 V for 45 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... 1:4 & 1:8) and loaded in triplicates onto a nitrocellulose membrane assembled in a 96-well dot-blot apparatus (Biorad, Cat#1703938). The membrane was then stained with Ponceau (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... Up to 8 replicate wells of up to 500 ng of DNA was added to ddPCR™ Supermix for Probes (No dUTP) (Bio-Rad) with a primer/probe set overlapping the LTR-gag junction designed to measure group-M HIV-1 proviral load (10) ...
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bioRxiv - Neuroscience 2020Quote: ... and 25-100 μg of total protein was loaded onto 8 to 15 % SDS-PAGE mini gels (Bio-Rad Laboratories, Hercules, CA) prior transfer onto PVDF membranes ...
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bioRxiv - Cell Biology 2020Quote: ... and 8 μg of protein was added from each sample to a 26-well Criterion TGX 4-20% precast gel (Bio-rad, 5671095). Proteins were transferred to an Immun-Blot low flouresence PVDF membrane (Bio-rad ...
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bioRxiv - Microbiology 2020Quote: ... and purified from remaining salts using Dowex 50 W X 8 50-100 mesh (Bio-Rad, 10 x 1 cm, H+ form) equilibrated with water ...
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bioRxiv - Cancer Biology 2020Quote: ... 50μg of proteins were electrophoresed in 8% or 12% polyacrylamide gel and then transferred to PVDF membranes (Bio-Rad Laboratory, CA, USA) overnight ...
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bioRxiv - Bioengineering 2022Quote: ... pH 8.3 at 100 V for 2 h at 2-8 °C using PowerPac Basic setup (Bio-Rad Laboratories Inc., Hercules, CA). Post-electrophoresis ...
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bioRxiv - Biophysics 2022Quote: ... Samples were centrifuged for 2 min at 12,000 x g before being separated on 8–16% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad) at 160 V for 60 min ...
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bioRxiv - Bioengineering 2023Quote: Cells were resuspended in regular culture media in 8-strip PCR tubes with 50□μl per tube and received heat shock (HS) in a thermal cycler (Bio-Rad, 1851148) for various durations before returning to normal culture condition ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were boiled at 100 °C for 5 min and loaded in handcrafted 8% SDS-polyacrylamide gel or 4-12% Criterion™ XT Bis-Tris XT Precast Gels (Bio-Rad) and electrophoresed in 1X Tris/Glycine/SDS buffer (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: Equal amounts of proteins were separated on 8% reducing SDS-PAGE and transferred to Nitrocellulose membranes (Ref# 162-0112, BIO-RAD, Germany). Primary antibodies anti-SERCA2a and anti-SERCA2b targeted the specific ends of SERCA2a and SERCA2b (see supplement) ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein pellet was resuspended in UTC buffer (8 M urea, 2 M thiourea, 4% CHAPS) and quantified using RC DC™ Protein Assay Kit (Bio-Rad). Sample with 80 µg proteins resuspended in rehydration buffer (8 M urea ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were separated by size by running 20 μg of the protein extract in 8% SDS-PAGE and transferred to Immuno-Blot PVDF Membrane (Bio-Rad, 1620174) using the Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Protein separation was done by 1D SDS-PAGE electrophoresis using commercial gels to ensure reproducibility (8–16% Criterion™ TGX™ Precast Midi Protein Gel, Bio-Rad). A sample of venom of ISm and ISy lines ...
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bioRxiv - Neuroscience 2020Quote: ... Diabetes-associated markers and cytokine levels in the plasma were assessed using the Bio-Plex Pro Mouse Diabetes 8-Plex Assay (Cat# 171F7001M; Bio-Rad, Carlsbad, California) and the Bio-Plex Pro Mouse Cytokine 23-Plex Assay (Cat# M60009RDPD ...
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bioRxiv - Microbiology 2020Quote: ... The precipitated proteins were then dissolved in 6 M urea/50 mM ammonium bicarbonate buffer (pH 8) for protein quantitation using DC™ protein assay (Bio-Rad Laboratories) and trypsin digestion.
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bioRxiv - Cell Biology 2020Quote: ... Total protein (5–20 μg) was resolved onto 8–12% SDS-PAGE and blotted onto 0.2 μm pore size PVDF membranes (1620177, Bio-Rad, Hercules, CA, USA). Primary antibodies were incubated overnight at 4°C after blocking membranes for 1h ...