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Citations for Bio-Rad :
251 - 300 of 675 citations for 7 Azaspiro 3.4 octane 6 8 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The protein was purified on a Bio-Spin® P-6 Gel Columns (BioRad) desalting column equilibrated in BC-0 buffer to separate labeled protein from excess fluorophore and then stored at −80 °C ...
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bioRxiv - Biophysics 2021Quote: ... and subsequently purified using Micro Bio-Spin P-6 gel columns (Bio-Rad 7326221). The BG-oligos were then conjugated to SNAP-proteins by mixing at a 2:1 molar ratio in storage buffer (20 mM HEPES [pH 7.5] ...
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bioRxiv - Molecular Biology 2019Quote: ... Gel exclusion chromatography on Bio-Gel P-6 acrylamide resin (Bio-Rad #150-0740) in renaturation and storage buffer RN#5 (0.1 M NaH2PO4 ...
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bioRxiv - Cancer Biology 2019Quote: ... The 6-well plates were imaged using a chemidoc (Bio-Rad, Hercules, CA, USA) and the number of colonies was counted in CellProfiler using an automated analysis pipeline ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons at DIV 4-6 were transfected using a biolistic gene gun (Bio-Rad) and were assayed 3 days after transfection as described previously (5,6,26,27) ...
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bioRxiv - Cancer Biology 2020Quote: ... Products were resolved via 6% PAGE and imaged using a ChemiDoc XRS (Bio-Rad). The percent spliced in (PSI ...
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bioRxiv - Biophysics 2021Quote: ... Free dye was removed by Micro Bio-Spin 6 Columns (Bio-Rad Laboratories, 7326222).
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bioRxiv - Genetics 2019Quote: ... Transcribed RNAs were then purified by micro Bio-Spin 6 chromatography columns (Bio-rad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Unquenched periodate was removed using Micro Bio-Spin P-6 columns (Bio-Rad Laboratories) according to manufacturer instructions ...
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bioRxiv - Biophysics 2023Quote: ... pH 7.5 using a Micro Bio-Spin™ 6 column (Bio-Rad 732-6221). The compound of interest (5 μM ...
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bioRxiv - Biophysics 2023Quote: ... with free dye removed using a Bio-Spin P-6 gel column (BioRad, 7326227) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... the samples were passed through a Micro Bio-Spin 6 Chromatography column (Bio-Rad), added to 445 μL Sonication buffer (20 mM Tris-HCl pH7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Free nucleotides were removed with BioRad Micro Bio-Spin 6 gel columns (BioRad 7326222) equilibrated to BRB80 buffer according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Aortic root sections (6 µm) were stained for CD68 (Bio-Rad MCA1957; Hercules, CA) to detect macrophages ...
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bioRxiv - Molecular Biology 2023Quote: ... the RNA was purified using Micro Bio-Spin™ P-6 Gel Columns (Biorad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... or homemade 6-15% SDS-PAGE gels and transferred to a nitrocellulose membrane (Biorad). The membrane was blocked in 10% non-fat dry milk in TBS-T and stained with primary antibody overnight at 4°C or 2-3 hours at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The reaction was cleaned up by P-6 Micro Bio-Spin Column (Bio-Rad). For fluorophore labeling ...
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bioRxiv - Immunology 2024Quote: RNA from 5×10^6 splenocytes was extracted with the RNeasy Mini Kit (BioRad) and converted to cDNA using the Maxima First Strand cDNA Synthesis Kit (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... The 10 µl reactions were purified with Bio-Spin® 6 Columns (Bio-Rad), and mixed with 10 µl of RNA loading dye (95% formamide ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 mM EDTA) using the Mini-PROTEAN Tetra Vertical Electrophoresis cell (gel size 10×8 cm, BioRad). Each run was preceded by a 30 min pre-run at 300 V to warm the gel ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were separated on an 8-16% gradient gel (Pierce) and transferred to a PVDF membrane (Biorad). The membrane was blocked in 5% milk for 1 h and incubated overnight with primary antibody (diluted 1:200 ...
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bioRxiv - Cancer Biology 2022Quote: ... histones were separated at 100V and 250mA on 8-16% Criterion TGX precast gels (Bio-Rad Laboratories). After fixation with a buffer containing 7% acetic acid and 10% methanol ...
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bioRxiv - Microbiology 2020Quote: ... and loaded into the wells of a polyacrylic gradient gel (8-16%) using a Kaleidoscope kit (BioRad) as a molecular weight marker ...
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Trypanosoma brucei J protein 2 functionally cooperates with the cytosolic Hsp70.4 and Hsp70 proteinsbioRxiv - Molecular Biology 2019Quote: ... and densitometric analyses of bands were conducted using the Image Lab v5.1 built 8 (Bio-Rad, USA).
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bioRxiv - Cell Biology 2021Quote: ... sample aliquots were used for protein separation on 8-16% TGX-Criterion gels (Bio-Rad, Hercules, CA). After electrophoresis ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal protein loads of 8ug were resolved on precast 8% to 16% Bis-Tris gels (Bio-Rad) under reducing conditions ...
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Negative curvature-promoting lipids instruct nuclear ingression of low autophagic potential vacuolesbioRxiv - Cell Biology 2021Quote: ... 10–15 μL of this supernatant was loaded onto a commercial 3–8% acrylamide gradient gel (BioRad) and migrated 70 min at 150 V in 1x Tris-Acetate buffer ...
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bioRxiv - Cell Biology 2022Quote: ... 10–15 µL of this supernatant was loaded onto a commercial 3–8% acrylamide gradient gel (BioRad) and migrated 70 min at 150 V to separate Rad53 isoforms ...
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bioRxiv - Microbiology 2023Quote: ... 8% SDS) and subjected to immunoblot analysis using the Stain-Free Imaging Technology (BioRad, Hercules, CA, USA). After SDS-Page ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were separated by SDS-PAGE in an 8-16% gradient Tris/Glycine gel (Bio-Rad #5671105) then transferred onto a nitrocellulose membrane by using the Trans-Blot Turbo RTA transfer kit (Bio-Rad #1704271) ...
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bioRxiv - Plant Biology 2023Quote: ... and 8 µL of the supernatant were loaded onto a precast mini-PROTEIN TGX Polyacrylamide Gel (BIORAD). After running for around 100 min at 160 V ...
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bioRxiv - Microbiology 2023Quote: ... were electrophoretic separated on Mini-PROTEAN TGX Precast Gels (8-16% polyacrylamide, Bio-Rad Laboratories, California, USA) at 70V for 15 min ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 8) and fractions analyzed via 12 % SDS-PAGE followed by Bio-Safe Coomassie Stain (Biorad,1610787). The peak fractions were combined and dialyzed in dialysis buffer (50 mM Tris pH 7.6 ...
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bioRxiv - Biochemistry 2024Quote: ... The eluate was analysed by SDS-PAGE using 3-8% Criterion XT Tris-Acetate gels (Bio-Rad) and XT Tricine running buffer (Bio-Rad) ...
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bioRxiv - Developmental Biology 2020Quote: ... 30 second at 72°C and a final extension of 7 minutes at 72 °C in a thermal cycler (T100TM Thermal Cycler, Bio-Rad). Five μl of PCR product was visualized on a non-denaturing 1% agarose gel with a 100bp ladder ...
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bioRxiv - Neuroscience 2021Quote: ... 40 min) and were subsequently transferred to nitrocellulose membranes for semi-dry immunoblotting (25 V, 1,3 A, 7 min, Bio-Rad®). Membranes were dried 5 min at 37°C and then incubated with 5% non-fat dried milk in Tris-buffered salineTween-20® (TBST containing 28 mM Tris ...
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bioRxiv - Plant Biology 2020Quote: ... Plates were incubated for 30 °C for 5 or 7 days and imaged using a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: Two micrograms of each RNA sample was loaded on 10% polyacrylamide gels containing 7 M urea or loaded onto 10% Criterion TBE-urea precast gels (Bio-Rad) and electrophoresed at 85 V ...
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bioRxiv - Pathology 2021Quote: ... Real-time PCR was performed on a QuantStudio 7 Flex Real-Time PCR System (Thermo-Fisher) using the iTaq Universal SYBR green Supermix (Bio-Rad). The following primer sequences were used ...
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bioRxiv - Molecular Biology 2022Quote: ... 20-30 μg of total protein was resolved on 8-16% TGX stain-free gels and transferred onto nitrocellulose membrane using the Trans-Blot Turbo RTA Transfer kit and the Trans-Blot Turbo Transfer system set to 7 min transfer protocol (all from Bio-Rad). Stain-free gel and blots were imaged using the Gel Doc EZ imaging system (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were transferred electrophoretically at a constant voltage of 25 V for 7 min using a Trans-Blot Turbo Transfer System (Bio-Rad). After transfer ...
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bioRxiv - Synthetic Biology 2020Quote: ... under constant electric current of 0.1 A per gel and voltage of 5-7 V using Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad, USA). Alternatively ...
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bioRxiv - Immunology 2021Quote: ... P2 was dialyzed against 1% glycine containing 2% ampholytes pH 3-10 (first run) or pH 5-7 (second run) and then loaded onto a liquid isoelectric focusing system (Rotofor, Bio-Rad). Individual fractions were harvested and their pH and absorbance at 280nm were measured ...
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bioRxiv - Immunology 2021Quote: ... pooled and subjected to two consecutive preparative isoelectric focusing (IEF) at pH 3-10 (first separation) and pH 5-7 (second separation) using a Rotofor Cell (Bio-Rad). The individual IEF fractions were harvested ...
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bioRxiv - Microbiology 2021Quote: ... Plates were incubated at 30 °C for 7 days and imaged daily using a gel documentation system (Gel Doc™ XR+, BIORAD). When needed ...
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bioRxiv - Biochemistry 2020Quote: ... the buffer of the purified DNAJB6 was exchanged into 10 mM ammonium acetate pH 7 solution using Micro Biospin P6 centrifuge columns (Bio-Rad) and protein concentration determined using a NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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Modifications in the T arm of tRNA globally determine tRNA maturation, function and cellular fitnessbioRxiv - Molecular Biology 2023Quote: ... RNA was transferred to a nylon membrane (Amersham Hybond-XL) at 23 V for 7 minutes using a Trans-Blot Turbo Transfer System (Bio-Rad). Following transfer ...
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bioRxiv - Cell Biology 2023Quote: Protein samples were resolved on 7-15% gradient polyacrylamide (SDS-PAGE) gels and transferred to nitrocellulose membranes via Trans Blot Turbo (Bio-Rad). Membranes were then blocked with 5% milk in Tris-buffered saline and 0.05% Tween 20 (TBST ...
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bioRxiv - Microbiology 2024Quote: Total RNA was loaded onto 8 to 12% PAGE–7 M urea gels and transferred to Hybond-N+ (GE) membranes using a Trans-Blot Turbo Transfer system (Bio-Rad) in 1X TBE ...
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bioRxiv - Molecular Biology 2020Quote: ... The adaptor was cleaned up on a Micro Bio-Spin 6 chromatography column (Bio-Rad) equilibrated with water ...