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Citations for Bio-Rad :
451 - 500 of 675 citations for 7 8 Dihydro 6 5H quinolinone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Flow cytometric staining and analysis was done as described [8] and acquired on ZE5 cell analyzer (Bio-Rad, Hercules, CA, USA). Analysis was performed using Flow Jo software.
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were separated by isoelectric focusing on immobilized linear pH gradient strips pH 5-8 for CDK4 (BioRad, Hercules, CA, USA) or pH 3–10 for CDK2 (Amersham Biosciences ...
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bioRxiv - Neuroscience 2019Quote: ... unless otherwise stated) were subjected to sodium docedyl sulfate-polyacrylamide (SDS-PAGE) electrophoresis on an 8% acrylamide gel (MiniProtean, Bio-Rad) including PageRuler Plus Prestained Protein Ladder (Thermo) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: PCR Products were separated on a 0.8% agarose gel and visualized after staining with DNA Stain Clear G (SERVA, Heidelberg) on a ChemiDoc imaging system (Biorad, Germany).
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bioRxiv - Synthetic Biology 2021Quote: ... 10nM of templates with each promoter type were added to TXTL reactions and incubated at 30 °C for 8 hours (T100 Thermal Cycler, Bio-Rad). Post-incubation ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed in Native NiNTA lysis buffer (50mM NaH2PO4, 300mM NaCl, pH 8, 10mM imidazole) and protein concentration was measured via Bradford assay (Bio-Rad). 500-800μg of protein were incubated with NiNTA beads at 4°C overnight ...
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bioRxiv - Genetics 2021Quote: ... or 1:8 (for all other quantifications) and 2ul was used to quantify gene expression using iTaq Universal SYBR Green Supermix (Bio-Rad) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... cell lysates were separated by 8% SDS-PAGE and Western blotted onto a PVDF membrane (semidry transfer, 15 V, 45 min, Trans blot Bio-Rad). The membranes were incubated overnight at 4°C in blocking solution 5% (w/v ...
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bioRxiv - Neuroscience 2021Quote: ... Thirty micrograms of total protein from each sample were loaded on 8% SDS-PAGE and transferred to PVDF membranes (Bio-Rad).
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bioRxiv - Immunology 2021Quote: ... Cell lysates were separated on 8-12% SDS-PAGE gel and transferred onto a PVDF membrane (Bio-Rad, Hercules, CA, USA). The membrane was incubated overnight with the respective primary antibodies at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... 50 μl of eluted samples were electrophoresed for 8 min at 200 V on a 10% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and protein was stained using Quick Blue Stain ...
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bioRxiv - Neuroscience 2022Quote: ... The pellet was resuspended in 50 μL 8M urea buffer (8 M urea, 100 mm NaHPO4, pH 8.5) and boiled in Laemmli sample buffer (BioRad, 161-0737) at 98°C for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... and treated with or without heat denaturation (96°C, 8 min) and ran on a TGX stain-free precast gel (Bio-Rad) for 38 min at 200 V ...
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bioRxiv - Neuroscience 2022Quote: ... and 500 ng RNA (RIN>8) was transcribed to cDNA using the iScript first strand cDNA synthesis kit (Bio-Rad 1708891). qPCR was carried out on a 384-well Quantstudio Flex system (ThermoFisher ...
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bioRxiv - Immunology 2022Quote: ... Equal volumes of diluted (1:8) cDNA were loaded for qPCR analysis along with the SsoAdvanced Universal SYBR Green Supermix (BioRad #1725272) and 0.5 μM forward and reverse primers for target genes (listed below) ...
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bioRxiv - Microbiology 2023Quote: ... Blots were washed 8 × 5 minutes in TBST and then ladder lanes were reacted with Precision Plus Strep Tactin HRP Conjugate (BioRad 1610380) diluted 1:10,000 in TBST ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by isoelectric focusing on immobilized linear pH gradient strips (pH 5 to 8, Bio-Rad, Hercules, CA, USA) before separation by SDS-PAGE ...
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bioRxiv - Cancer Biology 2023Quote: ... and then the gel was washed in destain buffer (8% acetic acid) until the background was washed off for visualization by ChemiDoc Imaging system (Bio-Rad).
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bioRxiv - Immunology 2023Quote: ... 8 µg protein per condition were separated by SDS-PAGE and blotted onto a 0.2 µm nitrocellulose membrane (Bio-Rad, #1704158) using a Trans-Blot® Turbo™ Transfer System (Bio-Rad) ...
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bioRxiv - Physiology 2023Quote: ... The protein extract (40μg) was separated on 8 or 10% polyacrylamide mini gel and transferred to a PVDF membrane (Bio-Rad, USA). Blotted membranes were incubated overnight at 4°C in SuperBlock(PBS ...
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bioRxiv - Bioengineering 2023Quote: We used conjugated magnetic beads (6.5 μm) (Bio-Plex Pro Human Chemokine IL-8 / CXCL8 Set #171BK31MR2, BioRad, Hercules, CA, USA) for the binding assay ...
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bioRxiv - Neuroscience 2022Quote: ... and then subjected to SDS polyacrylamide gel electrophoresis (PAGE) with 8% polyacrylamide and transferred to polyvinylidene difluoride (PVDF) membrane (BioRad Laboratories). The membranes were blocked for 1 h in Tris buffered saline with Tween 20 (TBST ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were boiled for 8–10 min at 95°C and loaded onto precast 12% Bis-Tris gels (Bio-Rad) with MES SDS running buffer (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: ... were separated by 8% acrylamide SDS-PAGE and transferred to a nitrocellulose membrane using the Trans-Blot Turbo transfer kit (Bio-Rad). 5% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... Aliquots of cell lysates (40-60 μg of protein) were subjected to SDS/PAGE on an 8 to 15% (vol/vol) acrylamide gel (MiniProtean; Bio-Rad) including PageRuler Prestained Protein Ladder (Thermo) ...
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bioRxiv - Immunology 2023Quote: ... Even amounts of each protein sample were separated on a 8–10% polyacrylamide gel using Mini-PROTEAN Tetra Cell electrophoresis system (Bio-Rad). Upon completion of electrophoresis ...
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bioRxiv - Microbiology 2023Quote: ... Parasites were incubated with drug for 8 or 12 h (short or long assays, respectively) and stained with 5 μg/ml ethidium bromide (EtBr, Bio-Rad) for 10 min followed by immediate flow-cytometry analysis (Becton Dickinson ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were heated at 95°C for 5 minutes and cooled to room temperature before being separated on an 8% tris-glycine SDS-PAGE gel and transferred to a nitrocellulose membrane using a semi-dry transfer machine (Bio-Rad). Membranes were blocked in 5% skim milk dissolved in Tris-buffered saline with 0.1% Tween-20 (TBST ...
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bioRxiv - Neuroscience 2023Quote: ... The pellet was resuspended in 8M urea buffer (8 M urea, 100 mm NaHPO4, pH 8.5) and boiled in Laemmli sample buffer (BioRad, 161-0737) at 98°C for 5 min ...
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bioRxiv - Immunology 2024Quote: ... Equal amounts of pre-cleared lysate samples were separated in 8 – 16% Mini protean-TGX stain free protein gel (Bio-Rad) and the gel was transferred to a Trans-Blot Turbo Mini-PVDF membrane (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... Approximately 20-30 μg of protein was resolved on 8% polyacrylamide gels and transferred onto nitrocellulose membranes using semi-dry Trans-Blot TurboTM (Bio-Rad). Following antibody incubation (anti-GFP ...
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bioRxiv - Plant Biology 2023Quote: ... The total proteins were separated by 8%-12% SDS-PAGE gels and blotted onto an Immune-Blot polyvinylidene difluoride (PVDF) membrane (Bio-Rad). All the antibodies were obtained from commercial suppliers (anti-GFP and anti-MYC antibodies from Roche ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein (20-30 μg) were resolved by 8 to 15% SDS PAGE MiniPROTEAN® TGX gels (Bio-Rad), from samples previously prepared with Laemmli Buffer 4X™ (Bio-Rad ...
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bioRxiv - Immunology 2019Quote: ... Rosa26LSL-tdTomato/+ animals (6-12 weeks) were given 1 μg of FITC-conjugated rat anti-CD169 antibody (BioRad) diluted in a total volume of 20 μl of PBS into the right footpad to label CD169+ subscapular macrophages inside the draining LN ...
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bioRxiv - Molecular Biology 2019Quote: ... 30 μL egg extract was applied to and eluted from a Micro Bio-Spin 6 column (Bio-Rad) equilibrated in ELB150 ...
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bioRxiv - Plant Biology 2021Quote: ... At least 4-6 technical replicate RT-qPCR reactions were performed using iTAQ with ROX and SYBR (BioRad), and 20μL reactions were prepared as per the manufacturer recommendations ...
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bioRxiv - Biochemistry 2022Quote: ... and 0.032 % (w/v) SM was applied to a centrifugal device (Micro Bio-Spin 6, Bio-Rad, Hercules) to exchange the buffer to a MS buffer containing 200 mM ammonium acetate (pH 7.4) ...
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bioRxiv - Biophysics 2022Quote: ... and 1 mM EDTA in 99.9% deuterium oxide using a spin desalting column (Micro Bio-Spin 6, BioRad). 15N-HSQC spectra were measured every 40 min for 106 hours at 25 °C on Bruker 600 MHz NMR spectrometer ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was purified by loading quenched samples onto Micro Bio-spin Columns with Bio-Gel P-6 (BioRad), followed by ethanol precipitation ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by 6 washes in 100 µL of PBST in Bio-Spin columns (catalog number 7326204, Bio-Rad). The bound immunocomplexes were eluted via 0.1 M glycine ...
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bioRxiv - Biochemistry 2021Quote: KirBac3.1 protein was buffer-exchanged to 200 mM ammonium acetate (pH=8.0) with 0.5% C8E4 (2×CMC) using a Biospin-6 column (BioRad). The protein sample was loaded into a gold coated needle prepared in-house and analysed on a modified Q-Exactive mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... then washed 6 x 5 minutes with TBS-T before developing with Clarity Western ECL Substrate (Bio-rad).
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bioRxiv - Biochemistry 2022Quote: ... solutions containing purified CytbX were buffer-exchanged into 0.2 M ammonium acetate (pH 8.0) containing 2x CMC of Lauryldimethylamine oxide (LDAO) using Biospin 6 columns (Biorad). 2-3 µL of 5 µM protein solution was introduced directly into Q-Exactive UHMR mass spectrometer (ThermoFisher ...
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bioRxiv - Biophysics 2022Quote: ... then 6 L of RO water with Ca2+ chelating beads (BIO-RAD Chelex 100 Resin Cat#142-1253), and finally 6 L of RO water alone ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was then cleaned 2-times on Micro Bio-Spin™ P-6 gel Columns (BioRad, #732-6221) where buffer was exchanged with phase separation buffer ...
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bioRxiv - Biochemistry 2022Quote: LiRecT protein was buffer exchanged into 100 mM ammonium acetate pH 7 (unadjusted) using Micro BioSpin P6 spin columns (Bio-Rad Laboratories, Hercules, CA, USA). All ssDNAs were dialyzed into 100 mM ammonium acetate with Pierce 96-well microdialysis devices with 3.5K MWCO (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2021Quote: ... before transfer onto a nitrocellulose membrane (Transblot Turbo Transfer Pack Midi 0.2 μm) for 7 min using the Transblot Turbo Transfer System (BioRad, program: Quickblot Mixed MW, Midi Gel). Membranes were incubated for 1 hour in blocking buffer (10% BSA ...
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bioRxiv - Physiology 2022Quote: ... Proteins were then either semi-dry transferred onto a polyvinylidene difluoride membrane at 1.3 A and 25 V for 7 min (Trans-Blot® Turbo™ Transfer System, Bio-Rad, Hercules, California, USA) or wet transferred onto nitrocellulose membrane at 100 V for 90 min (i.e. ...
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bioRxiv - Molecular Biology 2020Quote: ... carried out in glass tubes filled with polyacrylamide gel using a mixture of ampholytes 3/10 and 5/8 (Bio-Rad, USA) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... Electrofocusing was performed in glass capillaries with polyacrylamide gel using a mixture of ampholytes 3/10 and 5/8 (#163-1112, #163-1192, Bio-Rad, USA). The second direction is standard SDS 5-10% PAGE followed by staining with Coomassie Brilliant Blue G-250 (#31-4-58-1 ...