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Citations for Bio-Rad :
251 - 300 of 2968 citations for 6 Oxo 5 6 7 8 tetrahydronaphthalene 2 carbonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... 30 μl of purified protein in 0.02% DDM at ∼1 mg/ml was buffer exchanged into 200 mM ammonium acetate pH 7.5 and 0.02% DDM using Biospin 6 gel filtration spin columns (Bio-Rad, Hercules, CA). 2 μl of buffer exchanged ELIC was loaded into a borosilicate capillary emitter (Thermo Scientific ...
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bioRxiv - Microbiology 2019Quote: A549 or DF-1 cells in 6-well plates were infected with the indicated IAVs and cells were lysed with 2X Laemmli sample buffer (Bio-rad) plus 2% beta-mercaptoethanol at 8 hpi ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... were then generated using the piggyBac transposon system as described.12 Protein expression was induced with doxycycline and secreted fusion protein was separated from expression media using IgG Sepharose 6 Fast Flow resin (GE-Healthcare) in a 10 mL Poly-Prep chromatography column (Bio-Rad). Resin was washed with 50 column volumes of wash buffer (10 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2020Quote: ... The concentrations of mouse and human IL-6 and TNF-α were determined by a multiplex Bio-Plex assay (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... and 50 µl of a 10−4 dilution was loaded into selective ura− agar in a 6 cm petri plate and subjected to UVC radiation in a UVP gel Imaging and Documentation device (Bio-Rad) for 15 and 30 sec ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were dried out at RT and then exposed to a phosphoscreen cassette (GE) for 6 days for further scanning in a phosphoimager analyzer (Bio-Rad).
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bioRxiv - Genetics 2020Quote: ... chromosomal DNA plugs were prepared and separated on 1% agarose gel at 6 V/cm for 48 hrs (initial time = 20s, final time = 30s) by using the CHEF DRII apparatus (Bio-Rad), followed by Southern blotting and hybridization with a TRP1 sequence as a probe.
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bioRxiv - Genomics 2022Quote: ... and 20 ug of each sample were loaded on a 6% or 10% SDS-PAGE gel and transferred to a nitrocellulose membrane using Trans-Blot Turbo Transfer System (Bio-Rad). Western Blot was blocked using 5% ELK and incubated overnight with primary antibody mouse monoclonal anti-UHRF1 (Santa Cruz ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were grown at 30°C for 3-6 days and images captured using a Chemidoc XRS+ imager (BioRad, Hercules, CA). All images were evenly adjusted in Photoshop (Adobe Systems Incorporated ...
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β-catenin programs a tissue-specific epigenetic vulnerability in aggressive adrenocortical carcinomabioRxiv - Cancer Biology 2022Quote: Samples were prepared by boiling at 95°C for 6 minutes in lysis buffer supplemented with 4x Laemmli Sample Buffer (Bio-Rad), with 355 mM 2-mercaptoethanol freshly added according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The 6× His-tag blots were washed with TMST and TSM and developed with the Clarity Western ECL kit (Bio-Rad) and imaged in a Gel-Doc system (Bio-Rad) ...
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bioRxiv - Physiology 2023Quote: ... The incorporated radioactive methionine and cysteine signal was detected using Typhoon Imager (GE) and quantified using Image Lab 6 software (Bio-Rad). Afterwards ...
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bioRxiv - Cell Biology 2023Quote: Samples were diluted in 5x loading buffer and ∼30 mg run out on a 6-14% SDS-PAGE gel as described before semi-dry Western blotting (BioRad TurboID). Ponceau stained images were taken ...
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bioRxiv - Immunology 2023Quote: ... 10 µg of RNA was transfected into 4×10^6 S10-3 cells by electroporation with a Gene Pulser II apparatus (Bio-Rad) in 0.4 cm Gene Pulser cuvettes (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... Each sample was separated by non-reducing SDS-PAGE (6% acrylamide gel) with Precision Plus Protein Dual Color Standards (Bio-Rad) and transferred to nitrocellulose membrane overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... PBMCs isolated from two donors were infected with GFP-EBV for 28 days in 6-well plates and subsequent B-cells blasts were photographed using using a ZOE Fluorescent Cell Imager (BIO-RAD). Using similar strategy ...
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bioRxiv - Molecular Biology 2024Quote: ... or immunoprecipitated samples were resolved by SDS-PAGE (6% to 10%) and transferred onto methanol-treated immun-blot PVDF membrane (Bio-Rad). The membranes were incubated overnight at 4°C with corresponding primary antibodies (antibody information ...
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bioRxiv - Molecular Biology 2024Quote: ... The membrane was then incubated overnight at 4°C with a 1:1000 concentration of anti-6×His tagged monoclonal antibody produced in mouse (ABD Serotec Biorad, UK). Then ...
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bioRxiv - Immunology 2024Quote: ... The gel was imaged under the Silver Stain setting on Image Lab 6 on a Gel Doc EZ Imager (Bio-Rad). ImageJ was used to quantify band intensities and determine peptide loading efficiencies.
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bioRxiv - Neuroscience 2022Quote: ... 40 μg of samples 3 were mixed with 2× laemmli buffer + 5% 2-mercaptoethanol (BioRad Laboratories, Hercules, CA). Samples were then briefly vortexed and centrifuged ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... The protein extract was solubilized in 2-D lysis buffer (30 mM Tris-HCl pH 8.8, containing 7 M urea, 2 M thiourea and 4% CHAPS. Protein concentration was measured using Bio-Rad protein assay method ...
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bioRxiv - Plant Biology 2020Quote: ... Plates were incubated for 30 °C for 5 or 7 days and imaged using a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Synthetic Biology 2020Quote: ... under constant electric current of 0.1 A per gel and voltage of 5-7 V using Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad, USA). Alternatively ...
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bioRxiv - Immunology 2021Quote: ... P2 was dialyzed against 1% glycine containing 2% ampholytes pH 3-10 (first run) or pH 5-7 (second run) and then loaded onto a liquid isoelectric focusing system (Rotofor, Bio-Rad). Individual fractions were harvested and their pH and absorbance at 280nm were measured ...
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bioRxiv - Immunology 2021Quote: ... pooled and subjected to two consecutive preparative isoelectric focusing (IEF) at pH 3-10 (first separation) and pH 5-7 (second separation) using a Rotofor Cell (Bio-Rad). The individual IEF fractions were harvested ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were resuspended in a small amount of buffer (6 M urea, 50 mM Tris–Cl pH 7.5) and quantified by the BCA quantification method (Bio-Rad, Hercules, CA). Urea concentration was reduced to 4 M and the pH was adjusted to 8.8 with 25 mM Tris–HCl ...
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bioRxiv - Developmental Biology 2021Quote: ... Additional pre-absorption controls were performed in which the anti-IL-6 antibody was incubated overnight at 4 °C with a 10 fold molar excess of recombinant chicken IL-6 (1.67 μM; Bio-Rad Laboratories, Inc.) before immunolabeling fixed sections of chick ocular tissues ...
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bioRxiv - Neuroscience 2019Quote: ... 5uL of the sample was loaded into 4-6% polyacrylamide gel along with Precision Plus Protein™ Dual Color Standard (#1610374S, Bio-Rad) and was run at 120 V ...
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bioRxiv - Neuroscience 2021Quote: ... Membrane homogenates (50 μg) were separated on 6% SDS-PAGE gels and transferred onto presoaked PVDF membranes (Bio-Rad, Pleasanton, CA, USA). Blots were then blocked with 10% non-fat milk in PBST (100 nM phosphate buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein were run on 6% SDS-PAGE gels and blotted onto nitrocellulose using a semi-dry Trans-Blot (Bio-Rad, 1703940). Blots were incubated overnight at 4°C with a rabbit anti-myosin 18A polyclonal antibody that recognizes the C-terminal 18 residues of both myosin 18Aα and 18Aβ (Bruun et al. ...
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bioRxiv - Microbiology 2020Quote: ... pH 7.4) in D2O using Micro Bio-Spin® Columns with Bio-Gel® P-6 (Bio-Rad Laboratories, Hercules, CA, USA). To avoid aggregation ...
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bioRxiv - Bioengineering 2021Quote: ... were desalted and buffer exchanged into 10 mM ammonium acetate using a spin column (Micro Bio-Spin™ P-6 Gel Columns, Bio-Rad). Octylamine was added to aliquots of NCB solution at a concentration of 0.1% (v/v) ...
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bioRxiv - Biochemistry 2022Quote: ... pH 7.6 with HCl) for one hour and then divided for blotting on the basis of the prestained protein ladder (Bio-Rad, 1610373). Primary antibodies (see antibody table for vendor and dilutions ...
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bioRxiv - Molecular Biology 2022Quote: ... The bombardment was performed (a distance of 6 cm using 1350 psi rupture disks) with a PDS-1000/He Biolistic Particle Delivery system (Bio-Rad, USA). The antibiotic concentration was determined after a sensitivity test ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... was homogenized in 10 mM sodium acetate buffer (500 μL, pH 6.5) and filtered through Bio-spin 6 column (Bio-Rad, Hercules, CA) prewashed with 10 mM sodium acetate buffer (pH 6.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Purification of the desalted bacteriocin protein was performed using an Uno S-6 prepacked monolith cation exchange column (12×55 mm, Bio-Rad, USA) on a FPLC system (BioLogic DuoFlow System ...
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bioRxiv - Biochemistry 2020Quote: ... the desalted protein fraction was purified using the Uno S6 prepacked monolith cation exchange column (12 × 53 mm, 6 mL, Bio-Rad, USA). The eluted fraction containing purified bacteriocin was concentrated to 4.6 mg/mL in an Amicon centrifugal filter concentrator with a 3 kDa cutoff membrane (Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... SDS-PAGE and size exclusion chromatography (SEC) using a Superose® 6 Increase 10/300 GL column (Cytiva) and an NGC Quest 10 Plus System (Bio-Rad) showed that both wild-type and variant proteins were pure and monodisperse (Figure 2-figure supplement 1).
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bioRxiv - Microbiology 2023Quote: ... Tissues were rinsed in DBPS and moved to semi-solid media (6:4 ratio of 1% agarose (BIO-RAD, Hercules, CA, USA) in DPBS:DMEM with 10% FBS) ...
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bioRxiv - Pathology 2023Quote: ... Heparin Sepharose 6 Fast Flow beads (1 ml) (Healthcare Life Sciences) were loaded into disposable Bio-Spin® chromatography columns (Bio-Rad) and packed with 2 ml of equilibration buffer (0.15 M NaCl in 25 mM HEPES ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The qPCR was performed with a QuantStudio 6 Flex system equipped with a 384-well block and the SspAdvanced Unversal SYBR® Green Supermix (#1725272, BioRad, Switzerland) under the following conditions ...
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bioRxiv - Plant Biology 2023Quote: ... and proteins were separated in SDS/urea–PAGE (15% polyacrylamide, 6 M urea) (Laemmli, 1970) using mini-protean apparatus (Bio-Rad USA). These separated proteins were transferred to a nitrocellulose membrane using trans-blot apparatus (Bio-Rad USA) ...
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bioRxiv - Microbiology 2024Quote: The tRNA modified product from MenT3 in vitro reactions underwent the following steps: it was initially purified using Bio-Spin® 6 Columns (Bio-Rad), followed by incubation with a putative exoribonuclease (10 µM ...
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bioRxiv - Genomics 2023Quote: ... The nuclei were isolated from cell homogenate by centrifugation and counted on an automated cell counter (TC20 BioRad, range 4-6 um). Transposition of 100k (weeks 1 ...
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bioRxiv - Developmental Biology 2022Quote: ... for 2-5 min and scanned with GS-800 densitometer (Bio-Rad).
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TNFR1/p38αMAPK signaling in Nex+ supraspinal neurons regulates sex-specific chronic neuropathic painbioRxiv - Neuroscience 2023Quote: ... Membranes were blocked for 2 hours in 5% non-fat milk (BioRad) in 1x TBS-T (10 mM tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... DNA fragments was separated on 20% denaturing acrylamide gel (5% crosslinker, 7 M urea, 1X TBE buffer) using Criterion™ cell apparatus (Bio-Rad) at 300 V for 40 to 60 minutes ...
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bioRxiv - Microbiology 2024Quote: ... 2 % fetal bovine serum and 8 % chelated fetal bovine serum using Chelex® 100 Resin (BioRad, Ref. 142-1253). To differentiate HaCaT cells ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were separated on 6-10 % SDS-polyacrylamide gels or 4-20 % Mini-PROTEAN TGX precast gradient gels (Bio-Rad, CA, USA) together with Precision Plus Protein Dual Color Standards (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... and their respective complexes with Hap40 protein were buffer-exchanged into 150 mM aqueous ammonium acetate (pH=7.5) by using P-6 Bio-Spin gel filtration columns (Bio-rad, Veenendaal, the Netherlands). The protein’s resulting concentration was estimated to be ~2-5 μM before native MS analysis ...