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Citations for Bio-Rad :
301 - 350 of 735 citations for 6 Fluoroindole 3 Acetonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The binding reaction was resolved on a 6% polyacrylamide gel in 0.5X TBE buffer (pH 8.0) and was semi-dry blotted (Transblot, BIO-RAD, USA) onto a positively charged nylon membrane (BrightStar ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein concentration of VSW-3 RNAP was determined by Bradford protein quantitative kit (Bio-rad), and protein purity and concentration were analyzed along with T7 RNAP (New England Biolabs ...
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bioRxiv - Biochemistry 2019Quote: ... with or without CLEC-2 antibody (3 μg/ml final concentration; Bio-Rad, Oxford, UK). The plate was incubated in the dark for the indicated time and the reaction was stopped by addition of 200 μl 1% ice-cold formalin ...
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bioRxiv - Synthetic Biology 2021Quote: Supernatant or periplasmic fractions were diluted 3:1 in 4× Laemmli sample buffer (Bio-Rad) and were boiled at 100 °C for 10 min ...
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bioRxiv - Immunology 2020Quote: ... Development was performed after 3 washes in TBS-T and using Clarity ECL reagents (BioRad) and automatic exposure set up on ChemiDoc imaging system (BioRad).
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bioRxiv - Microbiology 2020Quote: ... The membrane was then washed 3 times in TBST and once in TBS (Bio-rad) before being exposed to 2ml of Western Lightning Plus-ECL ...
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bioRxiv - Plant Biology 2022Quote: ... The membrane was washed 3 times before image acquisition by ChemiDoc Touch Imaging System (Biorad). sRNA libraries were constructed with QIAgen miRNA library (QIAgen #331502 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Membranes washed 3 × 5 minutes in TBST then incubated in ECL reagent (BioRad, 170-5061) and imaged using a Chemidoc MP (BioRad) ...
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bioRxiv - Biochemistry 2020Quote: ... The 3 mg/mL protein sample was injected onto NGC Liquid Chromotography System (Bio-Rad) equipped with a HiPrep Sephacryl S-500 HR column equilibrated in FPLC buffer and separated into 156 ...
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bioRxiv - Molecular Biology 2019Quote: ... in Tris-glycine-SDS running buffer or 3-8% Criterion XT Tris-acetate gels (BioRad) in Tris-acetate-SDS running buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein samples were diluted (3:1) with a 4x Laemmli sample buffer (Biorad, Cat# 1610747) and heated at 98 °C for 5 min ...
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bioRxiv - Molecular Biology 2019Quote: ... after 3-4 hrs the beads were passed through Econo-Pac chromatography columns (Bio-Rad) and washed with 20x bead volume of wash buffer in the following order ...
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bioRxiv - Cell Biology 2020Quote: ... Gene expression was measured with the BioRad CFX Man 3 (Bio-Rad laboratories, CA, USA). The measured gene expression was corrected with housekeeping gene Gapdh ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were then blocked with 5% skim milk or 3% blotting-grade blocker (BioRad 1706404) for 1 hr ...
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bioRxiv - Immunology 2021Quote: ... blocking was conducted with 100ul per well of 3% milk solution made from Bio-Rad nonfat dry milk powder and 1xPBS ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were separated by 3-8% or 8-16% SDS-PAGE (NU-PAGE or BioRad) and transferred to immobilon-P membranes (Millipore) ...
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bioRxiv - Biochemistry 2021Quote: ... Gels were dried (3 hr, 80 °C) on a Model 583 Gel Dryer (Bio-Rad) and exposed to a phosphor screen ...
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bioRxiv - Microbiology 2022Quote: ... listed in Table 3 on the CFX96 Touch Real-Time PCR Detection System (Bio-Rad). Gene expression was calculated using the ΔΔct method using hprt as a housekeeping gene and normalized to the average WT S ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cell-free reactions were boiled for 3 min in 2x Tricine buffer (Bio-Rad, #1610739), loaded in 16.5% Mini-PROTEAN polyacrylamide Tris/Tricine gels (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... The membrane was blocked with 3% skin-milk-powder in Tris-buffered saline (Bio-Rad) with 0.1% Tween® 20 ...
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bioRxiv - Genomics 2023Quote: ... 3-2) Protein Enrichment: The serum samples were processed with a ProteoMiner kit (Bio-Rad) and the protein concentration was determined using the BCA and fluorescence assays ...
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bioRxiv - Microbiology 2023Quote: ... probe 5’-TGCAGTCCTCGCTCACTGGGCACG-3’ using the following conditions on a CFX96 Real Time PCR (Biorad): 50°C for 5 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 3 µg protein was separated by SDS-PAGE gel electrophoresis (Bio-Rad) and transferred to 0.2 µm nitrocellulose membrane (Amersham™ Protran™ ...
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bioRxiv - Microbiology 2023Quote: ... 40 µg of proteins were loaded onto 3–8% precast polyacrylamide gel (Bio-Rad, 3450129). After migration ...
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bioRxiv - Bioengineering 2023Quote: ... for 1–3 hours at 100 V and then transferred to PVDF membrane (Bio-Rad) at 40 V for 2–8 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA bands were stained with 3 × GelRed and visualized on a Chemidoc imager (Bio-Rad).
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bioRxiv - Cancer Biology 2024Quote: ... bromophenol blue) was applied onto IPG strip (7 cm, pH 3-10, Bio-Rad, 1632000) for 24 h ...
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bioRxiv - Biochemistry 2024Quote: ... DNA bands were stained with 3 × GelRed and visualized on the Chemidoc imager (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... and qPCR was then performed on a Quantstudio 3 with iTaq Universal SYBR Green (Biorad). RQ values were calculated using a standard curve ...
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bioRxiv - Molecular Biology 2020Quote: ... The deamination reaction was then carried out by incubating in a thermal cycler for four cycles of 5 minutes at 70°C followed by 1 hour at 60°C and then desalted with Micro Bio-spin 6 chromatography columns (Bio-Rad). RNA was desulphonated by adding an equal volume of 1 M Tris (pH 9.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... IB analysis was performed after 6-7.5% SDS-PAGE or 4-20% Mini-PROTEAN TGX Precast Protein Gels (BioRad, Hercules, CA), with overnight incubation with a 1:1000 dilution of primary antibody and followed by a 1:5000 dilution of horseradish peroxidase-conjugated anti-rabbit or anti-mouse antibody (Jackson ImmunoResearch ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... antigen retrieval was performed in trypsin (pH 7.8) or 10 mM sodium citrate buffer (pH 6) and then incubated with F4/80 antibody (1:50; MCA497, Bio-Rad) or UCP-1 antibody (1:500 ...
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bioRxiv - Microbiology 2019Quote: ... The libraries were quantified and checked for amplifiable adapters using the Library Quantification DNA standards 1-6 (Kappa Biosystems, Wilmington, USA) with the SsoFast EvaGreen qPCR supermix (Bio-Rad) using 10 μL EvaGreen master mix ...
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bioRxiv - Bioengineering 2019Quote: Equal volumes of (GT)6-Cy5-SWCNT and FAM-tagged protein at 2X working concentration were added to a 96-well PCR plate (Bio-Rad) to a total volume of 50 μL ...
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bioRxiv - Bioengineering 2020Quote: ... Equal volumes of 10 mg/L (GT)15- or (GT)6-Cy5-SWCNTs and 160 mg/L protein were added to a 96-well PCR plate (Bio-Rad) to a total volume of 50 μL ...
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bioRxiv - Biophysics 2021Quote: ... beads were washed with wash buffer 6-8 times in poly-prep chromatography columns (cat. no. 7311550, BIO-RAD laboratories Inc). Protein was eluted using Ni-NTA elution buffer (50□mM NaPi pH 7.6 ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples (20 µg/lane) were electrophoresed on a 6–12% SDS polyacrylamide gel in a Mini-PROTEAN Tetra Cell (Bio-Rad) and subsequently transferred to a nitrocellulose membrane (Sartorius AG ...
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bioRxiv - Cell Biology 2021Quote: ... as previously described 58 and a custom Bio-Rad 6-plex based on the Human Inflammation Panel 1 (BioRad, Cat# 171AL001M). For both assays ...
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bioRxiv - Cell Biology 2021Quote: ... inside an anaerobic glove-box and the excess DTT was completely removed via a Bio-Gel P-6 DG gel-desalting column (Bio-Rad) in thoroughly de-oxygenated MOPS buffer (20 mM ...
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bioRxiv - Biophysics 2021Quote: ... DIV15-30 neurons were transfected 2-6 days before imaging via biolistic gene transfer (160 psi, Helios gene gun, Bio-Rad), as described in a detailed video protocol (Woods and Zito 2008) ...
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bioRxiv - Biochemistry 2022Quote: ... and purified by vertical gel electrophoresis on a 6% (60:1 Acrylamide/Bisacrylamide) native gel column using a 491 Prep Cell (Bio-rad) run at 10W and 4°C ...
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bioRxiv - Microbiology 2019Quote: ... 4 µl of the diluted DNA sample was added to 6 µl of a mixture containing SYBR Green Supermix (Bio-Rad) and gene-specific primers (0·4 µM total ...
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bioRxiv - Biophysics 2019Quote: ... 30 μl of purified protein in 0.02% DDM at ∼1 mg/ml was buffer exchanged into 200 mM ammonium acetate pH 7.5 and 0.02% DDM using Biospin 6 gel filtration spin columns (Bio-Rad, Hercules, CA). 2 μl of buffer exchanged ELIC was loaded into a borosilicate capillary emitter (Thermo Scientific ...
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bioRxiv - Microbiology 2019Quote: A549 or DF-1 cells in 6-well plates were infected with the indicated IAVs and cells were lysed with 2X Laemmli sample buffer (Bio-rad) plus 2% beta-mercaptoethanol at 8 hpi ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... were then generated using the piggyBac transposon system as described.12 Protein expression was induced with doxycycline and secreted fusion protein was separated from expression media using IgG Sepharose 6 Fast Flow resin (GE-Healthcare) in a 10 mL Poly-Prep chromatography column (Bio-Rad). Resin was washed with 50 column volumes of wash buffer (10 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2020Quote: ... The concentrations of mouse and human IL-6 and TNF-α were determined by a multiplex Bio-Plex assay (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... and 50 µl of a 10−4 dilution was loaded into selective ura− agar in a 6 cm petri plate and subjected to UVC radiation in a UVP gel Imaging and Documentation device (Bio-Rad) for 15 and 30 sec ...
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bioRxiv - Microbiology 2021Quote: ... cells were infected with SINV at an MOI of 2 and pictures were taken 6 hours post-infection using ZOE fluorescent cell imager (Bio-Rad). Proteins were collected with lysis buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... 2% (w/v) SDS and 6% (v/v) β-mercaptoethanol) and protein concentrations were quantified using Bradford Protein Assay (Bio-Rad). Equal concentrations of protein were fractionated on NuPAGE 4-12% Bis-Tris Protein Gels ...
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bioRxiv - Biochemistry 2021Quote: ... was incubated at 55°C for 2 h and then purified by 6% non-denaturing PAGE using a Prep Cell apparatus (Bio-Rad).