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Citations for Bio-Rad :
101 - 150 of 4379 citations for 6 Chlorotetrazolo 1 5 b pyridazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Nonspecific binding was blocked for 1 hr at RT with 5% BLOTTO (Bio-Rad) in Tris-buffered saline with 0.1% Tween (TBST) ...
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bioRxiv - Neuroscience 2020Quote: ... After 1 hour blocking in 5% non-fat milk solution (Bio-Rad 170-6404) at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... Membranes were blocked for 1 hr using 5% non-fat dry milk (Bio-Rad) and incubated with primary antibody overnight at 4 °C ...
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bioRxiv - Bioengineering 2019Quote: ... Membranes were blocked for 1 h with 5% fat free milk powder (Bio-Rad) in TBS + 0.05% tween-20 (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2019Quote: ... Nonspecific binding was blocked for 1 h at room temperature with 5% blotto (Biorad) in Tris-buffered saline with 0.1% Tween (TBST) ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were boiled for 5 min in 1 x Laemmli Sample Buffer (Bio-Rad) containing 5% β-mercaptoethanol ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was diluted 1:5 and SsoFast EvaGreen Supermix was used (BioRad, California, USA). BipA was used as a housekeeping gene.102 Each repeat was performed in a single qPCR plate ...
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bioRxiv - Microbiology 2020Quote: Surface Plasmon Resonance (SPR) binding experiments were carried out on a ProteOn XPR36 6×6 channels protein interaction array system (Bio-Rad). Proteins BRD4-BD1 an BRD4-BD2 were diluted to a final concentration of 50μg/ml in HEPES buffer 25 mM pH 7.0 and immobilized to a GLM chip using amine coupling ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was washed four times in TBS-T for 5 min each before incubation for 1 h with secondary antibody (1:2000 dilution of BioRad affinity purified mouse α-rabbit IgG conjugated to alkaline phosphatase ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was washed four times in TBS-T for 5 min each before incubation for 1 h with secondary antibody (1:2000 dilution of BioRad affinity purified goat α-rabbit or goat α-mouse IgG conjugated to alkaline phosphatase ...
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bioRxiv - Genetics 2020Quote: ... nduo-5 and ctb-1 with act-1 as control by iQ™ SYBR® Green Supermix (BIO-RAD, #1708880).
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bioRxiv - Developmental Biology 2022Quote: ... membranes were incubated in a 5 ml 1:1 A:B solution of Clarity Western ECL substrate (BioRad cat. #170-5060) for 5 minutes at room temperature with rotation ...
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bioRxiv - Cancer Biology 2020Quote: ... qRT-PCR was performed with C-kit variant 1 forward primer 5’-CAACAAAGAGCAAATCCATCCC-3’ and reverse primer 5’-CATCACAATAATGCACATCATGCC-3’ with iQ SYBR Green supermix (BioRad, Hercules, CA, Cat No. 1708882). The PCR program was as follows ...
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bioRxiv - Biophysics 2021Quote: ... sealed with optical quality sealing tape (Microseal® ‘B’ seal from BIO-RAD). Data were analysed using the software Origin 8.5 ...
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bioRxiv - Cell Biology 2020Quote: ... GAPDH and b-globin RNA was then quantified using digital droplet PCR (Biorad). Values in each eluate were normalized to the corresponding values in the input and the enrichment of GFP-RAB13 RNA over GAPDH was assessed.
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bioRxiv - Microbiology 2023Quote: ... The PCR plate was sealed with a Microseal ‘B’ seal (Bio-Rad, MSB1001) and spun to remove air bubbles ...
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bioRxiv - Molecular Biology 2024Quote: ... with Optical Microseal ‘B’ PCR Plate Sealing Film (Bio-Rad, cat. no: MSB1001). PowerUp SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... The deamination reaction was then carried out by incubating in a thermal cycler for four cycles of 5 minutes at 70°C followed by 1 hour at 60°C and then desalted with Micro Bio-spin 6 chromatography columns (Bio-Rad). RNA was desulphonated by adding an equal volume of 1 M Tris (pH 9.0 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... antigen retrieval was performed in trypsin (pH 7.8) or 10 mM sodium citrate buffer (pH 6) and then incubated with F4/80 antibody (1:50; MCA497, Bio-Rad) or UCP-1 antibody (1:500 ...
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bioRxiv - Microbiology 2019Quote: ... The libraries were quantified and checked for amplifiable adapters using the Library Quantification DNA standards 1-6 (Kappa Biosystems, Wilmington, USA) with the SsoFast EvaGreen qPCR supermix (Bio-Rad) using 10 μL EvaGreen master mix ...
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bioRxiv - Cell Biology 2021Quote: ... as previously described 58 and a custom Bio-Rad 6-plex based on the Human Inflammation Panel 1 (BioRad, Cat# 171AL001M). For both assays ...
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bioRxiv - Biochemistry 2022Quote: ... and purified by vertical gel electrophoresis on a 6% (60:1 Acrylamide/Bisacrylamide) native gel column using a 491 Prep Cell (Bio-rad) run at 10W and 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The membrane was then incubated overnight at 4°C with a 1:1000 concentration of anti-6×His tagged monoclonal antibody produced in mouse (ABD Serotec Biorad, UK). Then ...
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bioRxiv - Cell Biology 2020Quote: ... 0.01% (w/v) bromophenol blue) and loaded on a 5% poly-acrylamide (29:1, BioRad) 0.5 x TBE gel ...
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bioRxiv - Cell Biology 2020Quote: ... blocked for 1 hour in 5% non-fat milk in TBS-T (Bio-Rad, USA), and incubated with primary antibodies anti-ACE2 (1:1000 ...
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bioRxiv - Synthetic Biology 2020Quote: ... We used 5% (w/v) skimmed milk in 1× tris-buffered saline (1706435, Bio-Rad) as the blocking reagent ...
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bioRxiv - Biophysics 2023Quote: ... Membranes were then incubated with ECL solution diluted 1:5 in deionized water (BioRad #1705061) for 30 seconds ...
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bioRxiv - Neuroscience 2024Quote: ... serum samples were diluted 1:5 with Laemmli sample buffer (Bio-Rad Laboratories, Hercules, CA) and heated at 100 °C for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... pH6.9 using a micro biospin 6 column (Bio-Rad). The FOS sample was diluted to ∼500 µM with 0.5 M ammonium acetate ...
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bioRxiv - Microbiology 2020Quote: ... and Bio-Plex Manager software (v.6, Bio-Rad). The data were expressed as median fluorescence intensity (MFI ...
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bioRxiv - Cancer Biology 2019Quote: ... transferred onto PVDF membrane and probed with primary antibody (Table 1) at a dilution of 1:1000 prepared in 5% blotting grade blocker (Bio-Rad) for 12-16 h at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were transferred to a PDVF membrane for 1 hour at 100V and blocked for 1 hour (5% blotting-grade blocker (Bio-Rad) in PBS containing 0.05% Tween 20) ...
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bioRxiv - Neuroscience 2022Quote: Tissue sections stained by mRNA FISH and fixed primary neurons were washed with 1 x PBS and blocked in 1 x PBS supplemented with 5 % (vol/vol) goat serum (Bio-Rad), 1 % (wt/vol ...
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bioRxiv - Microbiology 2023Quote: ... Tissues were rinsed in DBPS and moved to semi-solid media (6:4 ratio of 1% agarose (BIO-RAD, Hercules, CA, USA) in DPBS:DMEM with 10% FBS) ...
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bioRxiv - Pathology 2023Quote: ... Heparin Sepharose 6 Fast Flow beads (1 ml) (Healthcare Life Sciences) were loaded into disposable Bio-Spin® chromatography columns (Bio-Rad) and packed with 2 ml of equilibration buffer (0.15 M NaCl in 25 mM HEPES ...
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bioRxiv - Biophysics 2024Quote: ... 5% acrylamide (BIORAD), 0.1% SDS ...
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bioRxiv - Molecular Biology 2023Quote: ... Plates were firmly sealed with Microseal ’B’ PCR Plate Sealing Film (Bio-Rad MSB1001), mixed several times by inversion and low-intensity vortexing ...
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bioRxiv - Molecular Biology 2023Quote: ... Table 1) was performed at 4°C overnight at a 1:1000 dilution in 5% nonfat dry milk (Bio-Rad No. 1706404). Incubation of HRP conjugated secondary antibody (different species ...
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bioRxiv - Developmental Biology 2023Quote: ... All cDNA samples were diluted 1:5 and 1 μL was used in quantitative PCR (qPCR) reactions with iTaq Universal SYBR Green Supermix (Bio-Rad, #1725124), according to manufacturer’s instruction and using the Applied Biosystems QuantStudio 7 flex qPCR System ...
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bioRxiv - Cell Biology 2020Quote: ... X-ray film (Figs 1-5) and the ChemiDoc MP Imaging System (Bio-Rad, cat#17001402) (Supplemental Fig 3).
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bioRxiv - Microbiology 2019Quote: ... Protein was applied at 1 ml/min to a 5 ml ceramic hydroxyapatite column (BioRad BioscaleTM) in the dialysis buffer ...
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bioRxiv - Microbiology 2021Quote: ... Protein was applied at 1 ml/min to a 5 ml ceramic hydroxyapatite column (BioRad BioscaleTM) in the dialysis buffer ...
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bioRxiv - Cell Biology 2020Quote: Protein bands intensities were quantified with Image Lab 6 (BioRad). Equal rectangles were drawn around the total protein gel lane and the band of interest ...
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bioRxiv - Biochemistry 2019Quote: ... BioSpin 6 desalting columns were purchased from BioRad (Hercules, CA). Diethylamine NONOate (DEA NONOate) ...
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bioRxiv - Developmental Biology 2021Quote: ... 6 μL of Sybr Green Super Mix 2X (Bio-Rad), 2,6 μl MQ H2O.
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bioRxiv - Molecular Biology 2020Quote: ... resolving gels between 6% and 15% acrylamide/bis-acrylamide (BioRad) were used ...
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bioRxiv - Cancer Biology 2019Quote: ... 6’,-diamidino-2-phenylindole (DAPI; BIO-RAD, Hercules, CA, USA) for 30’ at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Unreacted linker was separated with Bio-Spin 6 columns (BioRad), that were previously equilibrated in PBS ...
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bioRxiv - Biochemistry 2023Quote: ... the samples were desalted using Biospin 6 columns (BioRad, CA), equilibrated with 50 mM triethylammonium bicarbonate and 0.02%(w/v ...
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bioRxiv - Biochemistry 2022Quote: ... then desalted with Micro Bio-Spin 6 columns (Bio-Rad). To 22.5 μL of the resulting solutions was added KCl (final concentration 100 mM ...