Labshake search
Citations for Bio-Rad :
651 - 700 of 1368 citations for 5 METHYLPICENE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2023Quote: Western blot – The different protein lysates were fractionated on 5-12% SDS-PAGE precast gels (Bio-Rad) and transferred onto Trans-Blot Turbo Mini 0.2 µm PVDF membranes using the Trans-Blot Turbo Transfer System (Bio-Rad ...
-
bioRxiv - Molecular Biology 2023Quote: ... and incubating the samples at 95°C for 5 min in a thermal cycler (Bio-Rad, USA) and decreasing the temperature of 1.5°C/min until it reaches 25°C ...
-
bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated shaking for 1 hour at RT with 4% blocking solution (5% milk powder (BioRad) in TBS-T) ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Synthesized cDNA was diluted 1:10 and 5 µl mixed with SsoAdvanced Universal Sybr Green supermix (Biorad). qPCR was performed using the Biorad CFX96 PCR machine ...
-
bioRxiv - Cell Biology 2024Quote: ... 5% v/v 2-mercaptoethanol) and fractionated by 4-15% Tris-Glycine gel (Bio-Rad Cat#5678084) using Tris-Glycine running buffer (0.2M Tris-HCl ...
-
bioRxiv - Molecular Biology 2024Quote: ... Membranes were washed twice for 5 minutes with TBST and an enhanced chemiluminescence (ECL Bio-Rad 1705061) solution was applied ...
-
bioRxiv - Immunology 2024Quote: ... blocked with 5% FBS for 1 hour and incubated with anti TGN46 antibody (#AHP500GT, BioRad, 1:2000) at 4°C overnight ...
-
bioRxiv - Cell Biology 2024Quote: ... 5% β-mercaptoethanol) and resolved on Any kD Criterion TGX Precast Stain-free gels (5678124, Bio-Rad). Proteins were transferred to 0.2 µm PVDF membranes using a TransBlot Turbo Transfer System (Bio-Rad) ...
-
bioRxiv - Bioengineering 2023Quote: ... and 5 μL of each dilution was loaded into a 25 μL PCR reaction (Bio-Rad, #1863024). 23 μL of the PCR reaction was used to generate droplets (Bio-Rad ...
-
bioRxiv - Molecular Biology 2023Quote: ... Mitochondrial (5 μg) fractions and heart homogenates (25 μg) were denatured in 1X Laemmli Buffer (Bio-Rad) containing 2-Mercaptoethanol at 95°C for 10 min and separated by electrophoresis in Any kDTM Mini-PROTEAN TGX precast gel (Bio-Rad ...
-
bioRxiv - Cell Biology 2020Quote: ... TBST (50 mM Tris-HCl pH 7.4, 1% Triton X-100) with 5% non-fat milk (Bio-Rad) was used for blocking ...
-
bioRxiv - Genomics 2021Quote: ... The proteins were separated in the first dimension on 4-7 and 5-8 IPG strips (Bio-Rad). The strips were then loaded onto 8-20% gradient PAGE for separation on the second dimension ...
-
bioRxiv - Biophysics 2020Quote: HEK293T cells cultured in 12 cm dishes were transfected with 5 μg plasmid using Transfectin lipid reagent (Biorad) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... samples were incubated for 5 minutes at 95 °C in T100 Thermal Cycler (Bio-Rad, Hercules, CA, USA). Samples were separated in 12.5 % sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE ...
-
bioRxiv - Neuroscience 2020Quote: ... Protein samples were prepared to a final concentration of 5 μg/μl in 4x Laemmli buffer (Bio-Rad) containing 25 mM DTT ...
-
bioRxiv - Genetics 2021Quote: ... Samples were then diluted 1:5 and placed into a 96 well plate with Supermix (no dUTPs, BioRad) and the required target locus primers and MGB-NFQ probes (Applied Biosystems ...
-
bioRxiv - Biochemistry 2020Quote: ... 500 mM NaCl and 0.02% [w/v] NaN3) using Bio-Select SEC 250-5 column (BioRad, California, USA) with a fractionation range of 1000 - 250 000 Da ...
-
bioRxiv - Biochemistry 2020Quote: ... followed by electroblotting to a PVDF membranes and blocked in 5% non-fat dry milk (Biorad, Hercules, CA) in Tris-buffered saline + 0.05% Tween-20 (TBST ...
-
bioRxiv - Cancer Biology 2021Quote: ... Complementary DNA (cDNA) was synthesized with 1-5 μg of RNA using iScript cDNA synthesis kit (Bio-Rad). mRNA levels were measured using qRT-PCR with SYBR Green Master Mix (BioRad) ...
-
bioRxiv - Physiology 2021Quote: ... Relative NKA and NHE protein abundance (n = 5 per treatment) was quantified using the Image Lab software (version 6.0.1; BioRad) and normalized by the protein content in each lane.
-
bioRxiv - Biochemistry 2020Quote: ... or 5 µL of plasma was added to 8.25 µL 4x Laemmli Sample Buffer (Bio-Rad, 161-0747) and 0.75 µL 55 mM 2-mercaptoethanol (Gibco ...
-
bioRxiv - Cell Biology 2021Quote: ... Plates were incubated at 30°C for 3-5 days and photographs were taken by Chemi Doc (BioRad).
-
bioRxiv - Microbiology 2020Quote: ... Soluble protein suspension was loaded onto the Bio-Scale Mini Profinity IMAC Cartridge (5 mL; Bio-Rad Laboratories) connected to the NGC Scout Chromatography system (Bio-Rad Laboratories) ...
-
bioRxiv - Microbiology 2021Quote: ... Immunohistochemistry was performed on paraffin sections (5 μm) using antibody F4-80 (Bio-rad, France; diluted 1:100). Sections were incubated overnight at 4°C ...
-
bioRxiv - Neuroscience 2019Quote: Organotypic slices were biolistically transfected at 3-5 days in vitro(div) using a Helios GeneGun (Bio-Rad), bullets were prepared using PI4KIIα shRNA alone or in combination with either wild type or S9/51A shRNA resistant PI4KIIα ...
-
bioRxiv - Molecular Biology 2021Quote: ... The dialyzed sample was loaded onto a 5 mL Bio-Scale Macro-Prep High Q Column (Bio-Rad). The resin was washed with 20 mM Tris-HCl buffer containing 7.5 M urea ...
-
bioRxiv - Neuroscience 2022Quote: ... 40 μg of samples 3 were mixed with 2× laemmli buffer + 5% 2-mercaptoethanol (BioRad Laboratories, Hercules, CA). Samples were then briefly vortexed and centrifuged ...
-
bioRxiv - Genomics 2022Quote: ... 5 μl of the pre-amplified reactions were amplified by qPCR with SsoFast™ EvaGreen® Supermix (BioRad) and 0.25 μl (25 μM ...
-
bioRxiv - Microbiology 2020Quote: ... Plates were incubated at room temperature for 5 minutes and imaged using a UV imaging system (ChemiDoc, BioRad) to assess agglutination ...
-
bioRxiv - Cell Biology 2019Quote: ... 5 min) and incubated for 1 h at room temperature with goat anti-rabbit IgG (H+L) (BioRad). Membranes were washed and signal was detected using the ChemiDoc XRS+ (Biorad ...
-
bioRxiv - Plant Biology 2021Quote: ... heated to 60°C for 5 min and electrophoresed into a 12% Tris-HCl Criterion gel from BioRad at 50V for ∼20 min or until the dye front moved just below the bottom of the sample wells ...
-
bioRxiv - Neuroscience 2021Quote: ... 10-15 μg of protein was boiled at 95°C for 5 min in Laemmli buffer (Bio-Rad), separated on a 4–12 % SDS/PAGE gel and then transferred using the Transblot®-Turbo™ Transfer system (Bio-Rad) ...
-
bioRxiv - Microbiology 2020Quote: ... The membranes were incubated overnight at 4°C in blocking solution 5% (w/v) Blotting-Grade Blocker (BioRad) in PBS-Tween buffer (1× PBS ...
-
bioRxiv - Microbiology 2022Quote: The membranes were blocked with 5% (w/v) Blotting Grade powdered milk blocker (Bio-Rad Laboratories, Hercules, CA) and 0.5% (w/v ...
-
bioRxiv - Microbiology 2022Quote: ... Oligo-dT first strand cDNA were synthesized from 5 μg total RNA by iScriptAdv cDNA kit (Biorad, # 1725038) following the manufacturers recommendations ...
-
bioRxiv - Cell Biology 2022Quote: ... Samples were further denatured at 80 °C for 5 minutes and loaded in 12% acrylamide gels (Bio-Rad). Electrophoresis was carried in Tris-Acetate SDS running buffer (Bio-Rad ...
-
bioRxiv - Cell Biology 2022Quote: Equal volumes (5 µg) of the prepared co-IPs were separated by SDS-PAGE (4–15%, Bio-Rad) and transferred to PVDF membranes (Millipore) ...
-
bioRxiv - Physiology 2019Quote: ... qPCR was performed using Taqman probes for β-actin (assay #Mm02619580_g1) and MMP13 (assay #Mm00439491_m1 which targets exons 4-5) and using iQ SYBR Green Supermix (BioRad) with β-actin as the housekeeping gene (primer sequences given in Supp ...
-
bioRxiv - Genetics 2019Quote: ... and the PVDF membrane was blocked in 5% non-fat dry milk-based blocking-grade blocker (Bio-Rad) for 1 hr at room temperature ...
-
bioRxiv - Genetics 2019Quote: ... genomic DNA was extracted using 250 μL of 5% Chelex® 100 Resin (Bio-Rad laboratories, Hercules CA) and 3 μL of Proteinase K (20 mg/ mL ...
-
bioRxiv - Synthetic Biology 2019Quote: ... Samples were vortexed a 5 µL was loaded onto a mini-protean TGX stain free gel (Biorad, 4568095) alongside 5 µL of Precision Plus ladder (Biorad ...
-
bioRxiv - Developmental Biology 2020Quote: ... boiled 5 min and separated by SDS-PAGE on Mini-PROTEAN® TGX™ precast gels (Bio-Rad). Bound Pnr 1-291 was identified by Western-blot (1:5000 rabbit anti-HA polyclonal ...
-
bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked for 1 h at rtp with 5% (w/v) non-fat milk block (Bio-Rad) in 1x TBS (in mM ...
-
bioRxiv - Genetics 2020Quote: ... Then 5 μL of worms were mixed with 40 μL of electroporation buffer (Gene Pulser Electroporation buffer, Biorad) in 1.5 mL tubes ...
-
bioRxiv - Genetics 2020Quote: ... The protein was pulled down by a disposable column loaded with 5 ml Nuvia IMAC resin (BioRad Lab) at 1 ml min-1 ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples were left on ice for 5 min and then analyzed in a S3e Cell Sorter (Bio-Rad) with a laser excitation at 488 nm ...
-
bioRxiv - Physiology 2021Quote: ... Membranes were blocked 1 hour in skim milk 5% TBS-Tween buffer (TBST, Bio-Rad, 0.05% Tween 20), and incubated overnight with the primary antibodies diluted in blocking buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... incubated 5 min at 95 °C and separated on gradient gels (BioRad 4-12% TGX pre-cast gels). The modified proteins could be detected with an infrared imager (Odyssey ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... 2 μl of the cDNA sample were supplemented with 5 μL of SsoADV Universal SYBR Green Supermix (BioRad) mix 2X ...
-
bioRxiv - Immunology 2020Quote: ... and transferred onto a PVDF membrane (Thermo Fisher Scientific).The membranes were blocked by 5% skim milk in TBST (Tris-buffered saline /0.1% Tween 20, Bio-Rad) and then incubated with antibodies to collagen a1 (IV ...