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Citations for Bio-Rad :
1 - 50 of 5364 citations for 5 Chloro 1 benzothiophen 2 yl methanol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... and stained with antibodies to α-tubulin (YL 1/2; 1:1000, Bio-Rad) and centromeres (HCT-0100 ...
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bioRxiv - Molecular Biology 2020Quote: ... 5% methanol) using a trans-blot system (Bio-Rad). Blocking the PVDF membrane was performed by shaking in TBS-T (10 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were washed three times with 1X PBS for 5 min and developed with nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate in alkaline phosphatase buffer (Bio-Rad).
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bioRxiv - Cancer Biology 2020Quote: ... Alkaline phosphatase was developed with 5-bromo-4-chloro-3-indolyl phosphate and Nitroblue tetrazolium (BCIP/NBT; BioRad, Germany) as substrate ...
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bioRxiv - Biophysics 2020Quote: ... Color Development was obtained using the chromogenic substrate 4-chloro-1-naphthol (4CN, Bio-Rad) and H2O2.
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bioRxiv - Molecular Biology 2024Quote: ... The blots were washed again after secondary antibody incubation and developed using either nitroblue tetrazolium (NBT)–5-bromo-4-chloro-3-indolylphosphate (BCIP) for AP-conjugated secondary antibodies or the ECL substrate (BioRad) for the HRP labelled secondary antibody after incubating for about 10 to 15 mins at room temperature with gentle shaking ...
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bioRxiv - Developmental Biology 2023Quote: ... Transfer buffer was prepared as a 1X final solution by mixing 7:2:1 of ddH2O: methanol: 10X Tris/Glycine Transfer Buffer (Bio-Rad #1610734). Following transfer ...
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bioRxiv - Microbiology 2023Quote: ... were detected as dark spots after a 10-min reaction with 5-bromo-4-chloro-3-idolyl phosphate and nitro blue tetrazolium using an alkaline-phosphatase-conjugate substrate (Bio-Rad, CA, USA). SFUs were counted using the AID ELISpot Reader System (Autoimmun Diagnostika) ...
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bioRxiv - Microbiology 2021Quote: ... fixed with methanol supplemented with 2% H2O2 and incubated with a biotinylated anti-RSV antibody (Bio-Rad) in PBS containing 1% bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2022Quote: ... 60 mg ml-1 of methanol-activated Bio-Beads SM2 resin (Bio-Rad) was added and allowed to mix overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... at 150V for 1 hour then transferred onto methanol-activated PVDF membrane (BioRad). Following a 1 hour block in 5% powdered milk ...
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bioRxiv - Cell Biology 2020Quote: ... anti-alpha-Tubulin (rat, YL1/2, MCA77G, Bio-Rad, 1:2000 (Figure 5) or 1:10000 (other figures) ...
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bioRxiv - Biochemistry 2021Quote: ... The membrane was washed with the washing solution twice and then reacted with 4-chloro-1-naphthol (Bio-Rad Laboratories) in the HRP color development buffer (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2024Quote: ... The methanol was then aspirated and 1 drop of Pathfinder Chlamydia Culture Confirmation System (BioRad) was added to each well ...
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bioRxiv - Cell Biology 2023Quote: ... 20% methanol for 1 h at 0.38 A (Bio-Rad Mini Trans-Blot Cell apparatus) or dry transfer (programme 0 ...
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bioRxiv - Immunology 2021Quote: ... purified recombinant NP samples were loaded to a 15% SDS-PAGE and then transferred to a nitrocellulose membrane of 0.2 μm cutoff using Tris-Methanol buffer (25mM TRIS-HCl, 192 mM glycine, 20% methanol, 0.1% SDS) in a Semi-Dry system (BioRad). The NP-containing membrane was then divided in test strips.
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bioRxiv - Bioengineering 2024Quote: ... 1-5 µg of target protein was resuspended in 2× Laemmli Sample Buffer (Bio-Rad) with adding 1:20 Beta-mercaptoethanol and 15 µL of the mixture was added onto Any kD™ Criterion™ TGX Stain-Free™ Protein gel (Bio-Rad) ...
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bioRxiv - Molecular Biology 2022Quote: ... at a 1:5 w/w ratio for 2 hours before addition of 100 mg mL-1 Bio-Beads SM-2 resin (Bio-Rad) and then incubated overnight at 4 °C with gentle agitation to remove detergent ...
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bioRxiv - Cancer Biology 2022Quote: ... Gels were transferred to methanol-activated PVDF (BioRad) at 90V for 120 minutes ...
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bioRxiv - Biophysics 2024Quote: ... then diluted below the CMC of C12E8 with reconstitution buffer and added to 0.5 g methanol-activated Biobeads SM-2 (Bio-Rad; 1523920). The mixture was rotated end over end for 18 hours and then subject to room temperature size exclusion chromatography on a Superose 6 10/300 GL column ...
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bioRxiv - Pathology 2022Quote: ... with 5% 2-Mercaptoethanol (Bio-Rad, 161-0710) and subjected to electrophoresis ...
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bioRxiv - Biophysics 2024Quote: ... and incubated for 3 hours at 37 °C and 5% CO2 with GPMV-vesiculation buffer consisting of 25 mM methanol-free formaldehyde (FisherScientific, cat# 11586711) and 2 mM dithiothreitol (Biorad, cat# 1610610) dissolved in HBSS ...
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bioRxiv - Immunology 2023Quote: ... and transferred to methanol-activated PVDF membranes (Bio-Rad) by wet transfer ...
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bioRxiv - Cancer Biology 2020Quote: ... and 2 μl cDNA (diluted 1:5) using a CFX384 real-time system qRT-PCR machine (BioRad). Primers were designed using Benchling and purchased from Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... and supplemented with 5% 2-mercaptoethanol (Bio-Rad Laboratories) for 8 minutes at 100 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... Laemmli sample buffer (Bio-Rad #1610747, 5% 2-mercaptoethanol) was added to 20 µg protein and samples were boiled at 95°C for 5 minutes before being loaded onto a 10% SDS-polyacrylamide gel (Bio-Rad #4568034) ...
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bioRxiv - Cell Biology 2024Quote: ... containing 5% 2- mercaptoethanol (1610710, Bio-Rad, Hercules, CA) and heated at 95°C for 5 min to denature ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein was transferred to methanol-activated PVDF membranes (Bio-rad) by wet transfer (1x Pierce Transfer Buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Gels were then equilibrated in transfer buffer (20% methanol) (BioRad) and total protein was imaged using a ChemiDoc Imaging System (BioRad).
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bioRxiv - Microbiology 2020Quote: ... 15 % methanol (v/v) in distilled water) for 1 hour at 100 volts (Mini Trans-Blot Cell, Bio-Rad). The nitrocellulose membrane was blocked in blocking buffer (5% (w/v ...
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bioRxiv - Pathology 2024Quote: ... for 1 hr at 75 V in ice-cold 20% methanol in 1X Tris-glycine buffer (Bio-Rad, #1610771EDU).
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bioRxiv - Pathology 2024Quote: ... for 1 hour at 75 V using ice-cold 20% methanol in 1X Tris-glycine buffer (Bio-Rad, #1610771EDU). Following transfer ...
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bioRxiv - Microbiology 2024Quote: ... Approximately 2-5 µg of the non-denatured protein samples were diluted 1:1 with the native sample buffer (BIO-RAD #161-0738) and loaded into the polymerized gel ...
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bioRxiv - Microbiology 2024Quote: ... 1 % 2-Mercaptoethanol (Biorad)) and bead beat for 2 minutes at maximum speed (Biospec Mini Beadbeater) ...
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bioRxiv - Bioengineering 2022Quote: ... 20% methanol using a TransBlot Turbo Semi-Dry Transfer Unit (BioRad) for 7 min ...
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bioRxiv - Plant Biology 2021Quote: ... the gel was rinsed with acetic acid/methanol (Destain, Bio-Rad). Each lane was cut into 4 bands ...
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bioRxiv - Developmental Biology 2022Quote: ... and transferring to methanol-activated PVDF membranes (Bio-rad, cat# 1620177). Specific protein detection was performed using diluted primary antibodies as follows ...
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bioRxiv - Molecular Biology 2024Quote: Proteins were then transferred to methanol-preactivated PVDF membranes (Bio-Rad) for 2 hours at 200 mA ...
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bioRxiv - Molecular Biology 2024Quote: ... 20% SDS and transferred using Transfer Buffer (Running Buffer, 20% Methanol) for 1 h and 30’ on Nitrocellulose membrane (BioRad). Membranes were blocked in 5 % milk ((W/V ...
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bioRxiv - Cell Biology 2024Quote: ... Digested DNA was purified and 2–5 μg of DNA was run on a 1% PFGE agarose gel (Bio-Rad) in 0.5 × TBE buffer using the CHEF-DRII system (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... VNR was detected on cells fixed in cold methanol by CD51/61 immunocytochemistry (clone 23C6, 1:400; Bio-Rad, Oxford, UK). Resorption tracks on dentine discs were visualised by staining with 0.5% toluidine blue under reflected light ...
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bioRxiv - Neuroscience 2024Quote: ... via wet transfer at 30V for 1 hour at 4 °C in 10% Methanol Tris/Glycine buffer using a Criterion™ Blotter (BioRad). Blots were all blocked for 1 hour at room temperature using Intercept (TBS ...
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bioRxiv - Plant Biology 2024Quote: Proteins from SDS-PAGE gels were transferred onto a PVDF (polyvinylidene difluoride) membrane (pre-activated in methanol for 1 min) using Trans-Blot Turbo transfer system (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were transferred onto PVDF membranes (activated in methanol for 1 minute) using the Trans-Blot® TurboTM Transfer System (BioRad). Transferred membranes were washed in tris-buffered saline (TBS ...
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bioRxiv - Molecular Biology 2024Quote: ... for 30 min at 100 V in 20 % methanol Towbin buffer (BioRad). Labeled proteins were visualized on the PVDF membrane using a Storage Phosphor screen (Molecular Dynamics ...
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bioRxiv - Microbiology 2023Quote: ... and then transferred onto methanol-activated Immun-Blot PVDF membrane (Bio-Rad) using Mini Blot Module (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were transferred to methanol-activated polyvinylidene difluoride (PVDF) membranes (Bio-Rad) at 100 V for 1,5 h ...
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bioRxiv - Physiology 2024Quote: ... Proteins were then transferred to methanol-preactivated PVDF membranes (Bio-Rad Laboratories) for 2 hours at 200 mA ...
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bioRxiv - Cancer Biology 2024Quote: ... Transfer was done overnight in 10% methanol in Tris/Glycine buffer (BioRad) onto either PVDF or Nitrocellulose membranes ...
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bioRxiv - Physiology 2024Quote: ... Proteins were then transferred to methanol-preactivated PVDF membranes (Bio-Rad Laboratories) for 2 h at 200 mA ...