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Citations for Bio-Rad :
201 - 250 of 1534 citations for 5 β DIHYDRO 17 HYDROXYPROGESTERONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... Blocked membrane in 5% Blotting-Grade Blocker (BioRad) in TBS-T (50 mM Tris pH 7.6 ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were blocked in 5%-milk (Biorad 1706404) for 30 minutes and washed in PBS/Tween20-0.05% ...
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bioRxiv - Neuroscience 2022Quote: ... After being blocked in 5% milk (Bio-Rad), the membrane was incubated with primary anti-tau polyclonal antibody (Dako ...
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bioRxiv - Genomics 2022Quote: ... and 5 kbp ladder (BioRad,Cat #170–3624) were used as standards ...
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bioRxiv - Neuroscience 2020Quote: ... 5’-tetramethylbenzidine (TMB Peroxidase EIA Substrate Kit, Biorad), a stop solution (H2SO4 2M ...
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bioRxiv - Pathology 2022Quote: ... with 5% 2-Mercaptoethanol (Bio-Rad, 161-0710) and subjected to electrophoresis ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA extractions using a 5% Chelex (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... and 5% beta-mercaptoethanol (Bio-Rad, Hercules, CA) and separated by SDS-PAGE with NuPAGE 4-12% Bis-Tris 1.5 mm 15-well gels (Thermo Scientific) ...
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bioRxiv - Immunology 2020Quote: ... IL-7 (5 ng/mL; BioRad, Paris France), and IL-2 (10 ng/mL ...
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bioRxiv - Synthetic Biology 2021Quote: ... 5 μl of 4X Laemmli loading dye (BioRad) was added to 15 μl of either total lysate ...
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bioRxiv - Microbiology 2020Quote: ... 5 μl of 4X loading buffer (Bio-Rad) was added ...
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bioRxiv - Biochemistry 2022Quote: ... with ImageLab Version 5 (Bio-Rad, Hercules, CA). Each experiment has three independent repeats.
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bioRxiv - Neuroscience 2023Quote: ... 5 µL of Sosofast Eva Green (Bio-Rad) 2X master mix ...
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bioRxiv - Neuroscience 2022Quote: ... and blocked in 5% Blotting Grade Blotter (Biorad) diluted in Tris buffered saline (TBS ...
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bioRxiv - Microbiology 2023Quote: ... 5 μl of SYBR Green Supermix (Bio-Rad), and 2 μl of cDNA (100 ng/mL) ...
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bioRxiv - Neuroscience 2023Quote: ... 5% (w/v) NFDM (Biorad, cat no: 1706404) blocking was also performed for each antibody ...
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bioRxiv - Developmental Biology 2023Quote: ... blocked in 5% non-fat milk (Bio-Rad) in TBST and incubated in primary antibodies (1:5000 rabbit anti-GFP ...
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bioRxiv - Cancer Biology 2023Quote: ... and 5% Serva Blue G dye (Bio-Rad) in 1M aminocaproic acid/50mM Bis–Tris/HCl ...
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bioRxiv - Developmental Biology 2023Quote: ... Non-denaturing 5% polyacrylamide 1xTBE Criterion gels (BioRad) were pre-run 30 minutes before loading the reactions.
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bioRxiv - Immunology 2024Quote: ... 72°C for 5 minutes (BioRad, Hercules, CA). Genomic PCR was performed using the following primers ...
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bioRxiv - Synthetic Biology 2021Quote: Biotinylated and non-biotinylated protein antigens and OMVs were mixed with loading buffer containing β-mercaptoethanol and boiled for 10 min prior to loading onto Mini-PROTEAN TGX polyacrylamide gels (Bio-Rad). To determine protein purity ...
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bioRxiv - Molecular Biology 2021Quote: ... and 2% β-mercaptoethanol) and separated on a 10% gel by SDS-PAGE (TGX Stain-Free™ FastCast™ Acrylamide Solutions; BIORAD) or 4-15% gel (Mini-PROTEAN® TGX Stain-Free™ ...
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bioRxiv - Biochemistry 2021Quote: ... The treated lysates were mixed with the 2 × SDS-PAGE sample buffer with or without 100 mM β-mercaptoethanol and run on 12% Mini-PROTEAN TGX Precast Gel (Bio-Rad) without a boiling step ...
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bioRxiv - Biochemistry 2021Quote: ... the reactant was mixed with the 2 × SDS-PAGE sample buffer containing 100 mM β-mercaptoethanol and run on 12% Mini-PROTEAN TGX Precast Gel (Bio-Rad) without a boiling step ...
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bioRxiv - Developmental Biology 2020Quote: ... Arl4c or β-actin transcripts were quantified by real-time PCR using gene-specific primers and iTaq Universal SYBR Green Supermix (Bio-Rad). ΔCt (ß-actin Ct – Arl4c-Ct ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein samples were combined with the 4x Laemli sample buffer (900 μl of sample buffer and 100 μl β-Mercaptoethanol), heated (95°C, 5mins) and run on SDS-PAGE (protean TGX pre-casted gradient gel, 4%-20%, Bio-rad) and transferred to the Nitro-Cellulose membrane (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... TNF-β were detected by using the premixed 48-plex Bio-Plex Pro Human Cytokine Assay (Bio-Rad, Hercules, CA, USA; 12007283) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... or lacking (non-reducing) 100 mM β-mercaptoethanol and resolved by SDS-PAGE in a 4-20% Mini-PROTEAN TGX Precast Protein Gel (BIO-RAD). Proteins were transferred to nitrocellulose membranes using a Trans-Blot SD Semi-Dry Transfer Cell (BIO-RAD ...
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bioRxiv - Immunology 2021Quote: ... Parasite lysate was reduced with β-mercaptoethanol (BME) and separated via SDS-PAGE in 4-20% Mini-PROTEAN TGX pre-cast gels (cat # 4561096, Bio-Rad) before transfer to PVDF membrane using a Trans-Blot Turbo Mini PVDF Transfer Pack (cat # 1704156 ...
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bioRxiv - Cancer Biology 2023Quote: ... Between 40mg and 120mg of each protein sample was boiled in Laemmli buffer with 10% β-Mercaptoethanol and equal percentages of each sample were run on 4-15% polyacrylamide gels (BioRad, 4561086). Resolved proteins were wet-transferred to nitrocellulose membranes (Amersham) ...
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bioRxiv - Cancer Biology 2023Quote: ... All samples were mixed with loading buffer containing SDS and β-mercaptoethanol and run on homemade 15% or commercial 4-15% gradient acrylamide gels (Bio-Rad). Semi-dry transfer was performed on a Trans-Blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... The spun down lysates were measured for total protein using a BCA assay and 15 μg of protein was diluted in 2x Laemmli buffer containing β-mercapto ethanol (Bio-Rad) and heated to 95 °C for 5 mins ...
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bioRxiv - Microbiology 2024Quote: ... of input RNA was amplified using KSHV-specific unique coding sequence primers (53) or β-actin primers (54) in a CFX96/C1000 instrument (Bio-Rad). Reaction conditions were 10 mins 50°C for RT ...
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bioRxiv - Immunology 2022Quote: ... and mouse hypoxanthine-guanine phosphoribosyltransferase gene (Hprt) (forward, 5’-GGACTTGAATCAAGTTTGTG-3’; reverse, 5’-CAGATGTTTCCAAACTCAAC-3’) in a CFX96 Real-Time PCR Detection System (Bio-Rad). The qRT-PCR results were analyzed using the ΔCt method (relative expression ...
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bioRxiv - Microbiology 2019Quote: ... of total 16S rRNA gene copies was carried out in triplicate 10µL reactions with 200nM 891F(5’-TGGAGCATGTGGTTTAATTCGA-3’)/1003R(5’-TGCGGGACTTAACCCAACA-3’) primers using a BioRad CFX384 thermocycler with iTaqTM Universal Probes Supermix (BioRad 1725132) and probe 1002P ([Cy5]CACGAGCTGACGACARCCATGCA[BHQ3] ...
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bioRxiv - Genetics 2020Quote: ... 1 μL diluted cDNA (5 ng) in a total volume of 5 μL using a CFX384 Real-Time System (Bio-Rad). For each experimental sample (four source colonies ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were washed three times with 1X PBS for 5 min and developed with nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate in alkaline phosphatase buffer (Bio-Rad).
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bioRxiv - Neuroscience 2023Quote: ... Membranes were washed 5 X 5 min in TBST and signals were detected using the Clarity Western ECL Substrate (Biorad #1705060). At least 2 separate gels were immunoblotted with cortical extracts from independent litters and used for quantitation.
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bioRxiv - Microbiology 2022Quote: ... qPCR was performed by mixing 5 μl of 5-times diluted cDNA with 10 μl of iTaq Universal SYBR Green mix (Bio-Rad), 3.6 μl of water and 0.8 μl of forward and reverse primers listed in Supplementary Table 1 ...
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bioRxiv - Microbiology 2023Quote: ... US28 (sense, 5’-CCAGAATCGTTGCGGTGTCTCAGT-3’; antisense, 5’-CGTGTCCACAAACAGCGTCAGGT-3’) and GAPDH were detected with CFX Connect Real Time System (Bio-Rad) using iTaq Universal SYBR Green Supermix (Bio-Rad) ...
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bioRxiv - Physiology 2023Quote: ... Lysates were then incubated for 5 min at 37 °C and 5 min at 75 °C in a thermocycler (T100, Bio-Rad), immediately followed by storage at -80 °C until further processing.
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bioRxiv - Microbiology 2023Quote: ... and primer set previously described [25] CtPl+ 5’-TAGTAACTGCCACTTCATCA-3’ and CtP2 5’-TTCCCCTTGTAATTCGTTGC-3’) and using a CFX96TM real-time thermocycler (Bio-Rad)) ...
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bioRxiv - Neuroscience 2019Quote: ... The supernatant was collected and equal parts of the supernatant and Laemmle sample loading buffer with β-mercaptoethanol (Bio-Rad, Hercules, CA, USA) was added for each sample ...
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bioRxiv - Immunology 2021Quote: ... Expression of negative co-stimulatory molecules together with β-actin and GAPDH endogenous controls were analyzed using CFX96 Touch Real-Time system (BIO-RAD Inc.) in 96-well MicroAmp® optical fast-plates (Applied Biosystems® ...
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bioRxiv - Neuroscience 2020Quote: ... 20 μg samples were boiled in gel loading buffer containing β-mercaptoethanol as a reducing agent and loaded onto a 12% or “AnyKD” precast polyacrylamide gel (Bio-Rad 4569033) for western blot analysis ...
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bioRxiv - Developmental Biology 2020Quote: ... protein samples were boiled with β-mercaptol and ran in SDS-PAGE gels casted from 30% Acrylamide/Bis solution 29:1 (Bio-rad, 1610156) using the Mini-PROTEAN® system (Bio-rad) ...
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bioRxiv - Genetics 2019Quote: ... Total protein was quantified using the BCA assay and 25-50μg of protein in 1x Lamelli Buffer with β-mercaptoethanol was loaded onto 4-15% TGX protean gels (Bio-Rad, Hercules, CA). The protein was transferred to a .2μm PVDF membrane by wet transfer ...
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bioRxiv - Neuroscience 2023Quote: ... 20µg samples were boiled in gel loading buffer containing β-mercaptoethanol and sodium lauryl sulfate as a reducing agent and loaded onto AnyKD precast polyacrylamide gels (Bio-Rad 4569033) for protein separation ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: mMEC and hMEC samples were directly homogenized using 2x Laemmli Sample Buffer and supplemented with 2-Mercaptoethanol (β-mercaptoethanol) (BioRad Hercules, California – USA). Proteins were separated by electrophoresis on a polyacrylamide gradient gel (BioRad Hercules ...
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bioRxiv - Plant Biology 2020Quote: ... The mixture was kept for 5 min at 95°C and 5 min on ice before loading on a TGX 4-20% Strainfree (Bio-Rad US) gel immersed in TGS 1X buffer ...