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Citations for Bio-Rad :
601 - 650 of 1009 citations for 5α CHOLESTAN 3 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... to allow detection of both in one reaction droplet ddPCR was performed with a BioRad QX100 ddPCR machine using the recommended protocols and reagents (BioRad Labatories, Hercules, CA). ddPCR was performed on biological quadruplicates of males and females ...
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bioRxiv - Microbiology 2024Quote: ... one-step reverse transcription droplet digital PCR (RT-ddPCR) assays were performed with the One-step RT-ddPCR Advanced Kit for probes (Bio-Rad Laboratories Ltd.), using primers and probes targeting 2’,5’-oligoadenylate synthetase 1 (OAS1) ...
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bioRxiv - Neuroscience 2019Quote: ... capacitance of 3 μF and a resistance of 400 Ω (Gene Pulser II (BioRad)) ...
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bioRxiv - Genomics 2020Quote: Lysates were separated by SDS-PAGE on 3-8% Tris-acetate gels (BioRad #3450129) for 2.5 hours at 150V ...
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bioRxiv - Biophysics 2021Quote: ... Detergents were removed by adding 2-3 batches of Bio-beads SM2 (Bio-Rad) with constant rotation for overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... and 3 µl was used for qPCR with iTaq Universal SYBR Green Supermix (BioRad). Relative levels of RNA were calculated by the ΔΔCt method using GAPDH as an internal control ...
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bioRxiv - Microbiology 2021Quote: ... The nitrocellulose membrane was soaked (blocked) in 3% milk (dry milk from Bio-Rad) solution in 1x PBS following by incubation with appropriate primary antibody solution (1:1000 dilution ...
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bioRxiv - Evolutionary Biology 2022Quote: ... washed 3 times and developed with ECL reagent (Bio-Rad Laboratories, Hercules, CA, USA). Invertase blots were prepared similarly with an anti-invertase antibody and anti-goat IgG HRP ...
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bioRxiv - Molecular Biology 2021Quote: ... SDS-PAGE and Western blotting were performed using the Mini-PROTEAN 3 system (Biorad). Blotted membranes were blocked in 5% BSA / TBS-Tween ...
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bioRxiv - Developmental Biology 2023Quote: ... Following separation via SDS-PAGE (12%, Mini PROTEAN 3 System, Bio-Rad, Hercules, CA), proteins were transferred to PVDF membrane for 1hr using a Genie electroblot chamber (Idea Scientific ...
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bioRxiv - Physiology 2023Quote: ... Blotting membrane was primed for 3 min prior in cold transfer buffer (BIORAD 10026938), according to manufacturer specifications ...
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bioRxiv - Evolutionary Biology 2023Quote: ... kinase-dead extracts were incubated 3 consecutive times with protein A-beads (Bio-Rad) previously coated with the anti-B55δ antibody (1 hour incubation at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... samples were run through Criterion XT Tris-acetate precast gels (3-8% gradient) (Biorad) in a XT tricine buffer (Biorad) ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were loaded onto a 3% agarose gel and visualized on a Chemidoc (BioRad). Bands were densitometry quantified by ImageJ and the percentage of methylated DNA was calculated over the total intensity of all the quantified bands.
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bioRxiv - Molecular Biology 2023Quote: ... 3 μg of the protein samples were prepared with 4X Laemmli loading buffer (Biorad) and denatured at 95°C for 5 minutes ...
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bioRxiv - Immunology 2023Quote: ... 3) live cell populations using live-dead exclusion dye (Zombie Aqua, #423101, Bio-Rad); 4 ...
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bioRxiv - Molecular Biology 2023Quote: ... SDS-PAGE and Western blotting were performed using the Mini-PROTEAN 3 system (Biorad). Blotted membranes were blocked in 5% BSA / TBS-Tween ...
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bioRxiv - Immunology 2024Quote: ... Samples were diluted in Laemmli sample loading buffer supplemented with |3-mercaptoethanol (Bio-Rad), heated for 5 min at 95°C ...
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bioRxiv - Biophysics 2022Quote: ... Experiments were performed in triplicate and densitometry was carried out using a DNA standard curve and Quantity One® software (Bio-Rad, Hercules, CA). The values reported in Figure 2 were calculated by dividing the portion of Oct4-nucleosome complex by the value of starting free nucleosome (control) ...
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bioRxiv - Molecular Biology 2020Quote: ... Chemiluminescence was detected with an ImageQuant Las 4000 instrument (Cytiva, Little Chalfont, UK) and quantified using Bio-Rad Quantity One software (Bio-Rad, Hercules, CA, USA). For a reference standard we used an in-house produced monoclonal antibody of known concentration.
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bioRxiv - Developmental Biology 2020Quote: ... and one-dimensional electrophoresis was carried out for 4 hours at 75-100 volts in a Tris-glycine-SDS (Bio-Rad, catalog 161-0732) running buffer ...
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bioRxiv - Microbiology 2021Quote: The 20 μl RT-dPCR reaction mixture consisted of 5 μl One-Step RT-dPCR Advanced Supermix for Probes (Bio-Rad, Laboratories, Munich, Germany), 2 µl of Reverse Transcriptase (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... One μg RNA was reverse-transcribed using oligo(dT)18 and iScript Advanced cDNA synthesis kit for RT-qPCR (Bio-Rad Laboratories; Hercules, CA). Il1r1/IL1R1 and Gapdh/GAPDH qPCR was performed using specific primers and Lightcycler 480 Sybr Green I Master (Roche ...
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bioRxiv - Microbiology 2021Quote: ... The amount of viral RNA expressed as TCID50 equivalent per insert (TCID50e/insert) was quantified by RT-qPCR using iTaq universal probes one-step kit (Bio-Rad, catalogue no. 1725141), and a commercial mix of primers for N gene (forward primer 5’-GACCCCAAAATCAGCGAAAT-3’ ...
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bioRxiv - Biochemistry 2022Quote: ... the HRP signals were visualized by using Chemi-Lumi One Super (02230-30, Nacalai Tesque) and a ChemiDocTM Imaging System (Bio-Rad Laboratories, Hercules, CA). The primary antibodies used in this study were anti-β-actin antibody (M177-3 ...
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bioRxiv - Microbiology 2020Quote: ... IL-22 and TNF-α was analyzed by using quantitative RT-PCR (qRT-PCR) according to the Biorad iScript™ One-Step RT-PCR Kit in a CFX96 apparatus (Bio-Rad, Hercules, CA) with the following primers ...
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bioRxiv - Cell Biology 2021Quote: ... or mRNA isolated from cell lines or primary mouse cells were proceeded to RT-qPCR using iTaq universal SYBR green one step kit (Bio-Rad, Cat. No. 1725150). DNA obtained from ChIP was proceed to RT-qPCR using iQ SYBR green supermix (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... ECL substrate was added according to manufacturer’s instructions after which chemiluminescence was analyzed in a Molecular Imager ChemiDoc XRS by Quantity One Software (Bio-Rad Laboratories, Hercules, CA, USA).
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bioRxiv - Cancer Biology 2020Quote: The ddPCRs assay comprised a total volume of 20 μL containing on the one hand the droplet Supermix (Bio-Rad Laboratories, Richmond, CA, USA) at final concentration of 500 nM of forward and reverse primers and 250 nM of VIC- and FAM-labeled probes and in the other hand the DNA input containing 8 µL of eluted cfDNA (mean ...
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bioRxiv - Physiology 2019Quote: ... Defined amounts of mRNAs at 10-fold dilutions were amplified via real-time PCR using iTaq™ Universal SYBR® Green One-Step kit (Bio-Rad, Italy) using the following RT-PCR conditions ...
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bioRxiv - Immunology 2021Quote: ... Reverse transcription followed by qPCR was performed in the same reaction using the Universal Probes One-Step PCR kit (Bio-Rad Laboratories, Hercules, CA) and the Taqman primers Cftr (Mm00445197_m1) ...
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bioRxiv - Cell Biology 2023Quote: ... They were then washed thrice with TBST (5 minutes/wash) at room temperature and signal developed for one minute using Clarity™ Western ECL substrate (Bio-Rad, Cat #: 1705060) or Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were stained with coomassie blue R250 and PMMoV-I coat protein was quantified using a densitometer and the quantity one software (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Genomics 2022Quote: ... All PCRs described above were carried out using KOD One Master Mix (#KMM-101, TOYOBO, Osaka, Japan) with a C1000 Touch thermal cycler (Bio-Rad, Hercules, CA, USA). Primer sequences are listed in Table S3.
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bioRxiv - Immunology 2023Quote: ... The viral genome of the H7N1 virus was quantified using qRT-PCR that was conducted with the Bio-Rad iTaq™ Universal SYBR ® Green One-Step Kit (BioRad, California, USA) using primers designed for the detection of the M gene as published elsewhere 60.
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bioRxiv - Immunology 2024Quote: ... as detailed in “RNA/DNA extraction and sequencing.” Kars RNA expression was measured in each tumor using the iTaq Universal SYBR Green One-Step Kit (Bio-Rad Cat. No. 1725150) and the Quantstudio 6 Flex System (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein concentration of VSW-3 RNAP was determined by Bradford protein quantitative kit (Bio-rad), and protein purity and concentration were analyzed along with T7 RNAP (New England Biolabs ...
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bioRxiv - Biochemistry 2019Quote: ... with or without CLEC-2 antibody (3 μg/ml final concentration; Bio-Rad, Oxford, UK). The plate was incubated in the dark for the indicated time and the reaction was stopped by addition of 200 μl 1% ice-cold formalin ...
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bioRxiv - Synthetic Biology 2021Quote: Supernatant or periplasmic fractions were diluted 3:1 in 4× Laemmli sample buffer (Bio-Rad) and were boiled at 100 °C for 10 min ...
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bioRxiv - Immunology 2020Quote: ... Development was performed after 3 washes in TBS-T and using Clarity ECL reagents (BioRad) and automatic exposure set up on ChemiDoc imaging system (BioRad).
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bioRxiv - Microbiology 2020Quote: ... The membrane was then washed 3 times in TBST and once in TBS (Bio-rad) before being exposed to 2ml of Western Lightning Plus-ECL ...
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bioRxiv - Plant Biology 2022Quote: ... The membrane was washed 3 times before image acquisition by ChemiDoc Touch Imaging System (Biorad). sRNA libraries were constructed with QIAgen miRNA library (QIAgen #331502 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Membranes washed 3 × 5 minutes in TBST then incubated in ECL reagent (BioRad, 170-5061) and imaged using a Chemidoc MP (BioRad) ...
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bioRxiv - Biochemistry 2020Quote: ... The 3 mg/mL protein sample was injected onto NGC Liquid Chromotography System (Bio-Rad) equipped with a HiPrep Sephacryl S-500 HR column equilibrated in FPLC buffer and separated into 156 ...
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bioRxiv - Molecular Biology 2019Quote: ... in Tris-glycine-SDS running buffer or 3-8% Criterion XT Tris-acetate gels (BioRad) in Tris-acetate-SDS running buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein samples were diluted (3:1) with a 4x Laemmli sample buffer (Biorad, Cat# 1610747) and heated at 98 °C for 5 min ...
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bioRxiv - Molecular Biology 2019Quote: ... after 3-4 hrs the beads were passed through Econo-Pac chromatography columns (Bio-Rad) and washed with 20x bead volume of wash buffer in the following order ...
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bioRxiv - Cell Biology 2020Quote: ... Gene expression was measured with the BioRad CFX Man 3 (Bio-Rad laboratories, CA, USA). The measured gene expression was corrected with housekeeping gene Gapdh ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were then blocked with 5% skim milk or 3% blotting-grade blocker (BioRad 1706404) for 1 hr ...
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bioRxiv - Immunology 2021Quote: ... blocking was conducted with 100ul per well of 3% milk solution made from Bio-Rad nonfat dry milk powder and 1xPBS ...