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Citations for Bio-Rad :
1 - 50 of 119 citations for 18 0 PE square since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Gene expressions were normalized to 18S expression (18S universal primer; BioRad) using the ΔCt method ...
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bioRxiv - Cell Biology 2020Quote: ... square-wave pulses five seconds apart (Biorad GenePulser Xcell + CE module) for all samples ...
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bioRxiv - Microbiology 2022Quote: ... and 1’,3’-bis[1-palmitoyl-2-oleoyl-sn-glycero-3-phospho]-glycerol (16:0-18:1 Cardiolipin) were spotted using a Hamilton syringe onto a nitrocellulose membrane (Biorad trans-blot turbo RTA Midi 0.2 µm) to yield 10 ...
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bioRxiv - Biophysics 2024Quote: ... and pulse lengths of 50 ms using square waveform pulses (Bio-Rad). Subsequently ...
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bioRxiv - Cell Biology 2020Quote: ... precast 4-18% gradient gels (BioRad). Recombinant N-terminally acetylated α- ...
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bioRxiv - Cell Biology 2022Quote: ... or 18-well (Bio-Rad, #567-1034) gels ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 18s - Hs03003631_g1) and SsoAdvancedTM Universal Probes Supermix (BioRad #1725281). RNA sequencing was performed as described before21 by the Cincinnati Children’s Hospital and Medical Center DNA core with RNA polyA-stranded library preparation ...
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bioRxiv - Immunology 2020Quote: ... PE anti-LTB4R (BioRad, MCA2108PET), PE anti-CD35 (Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2020Quote: ... transferred onto a nitrocellulose membrane (BioRad, Cat. No. 9004-70-0) and detected using indicated antibodies.
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bioRxiv - Cancer Biology 2022Quote: ... and subjected to a single square wave pulse at 125 V for 20 ms (Gene Pulser Xcell, Bio-Rad). These juvenile flukes were maintained in culture in RPMI supplemented with 1% glucose for 60 min after which they were used for infection of hamsters by stomach gavage (below).
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bioRxiv - Immunology 2024Quote: ... was introduced by electroporation (7 square wave pulses of 30 V, 3ms, 0,1s pause) using a GenePulser Xcell electroporator (Biorad) and a 1 mm cuvette (Biorad) ...
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bioRxiv - Neuroscience 2021Quote: ... 18-well Criterion SDS-gels were used (Cat# 1704273, Biorad). Primary antibodies were diluted in 5% milk/TBST or 5% BSA/TBST as recommended ...
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bioRxiv - Biochemistry 2022Quote: ... cells were transferred to a 0.4 cm cuvet for a 10 ms pulse of 300 V on a BTX Square-Wave electroporator (Bio-Rad). Cells were rested 15 min at room temperature and then transferred to 10 ml antibiotic-free medium and incubated overnight ...
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bioRxiv - Genetics 2020Quote: ... Animals were electroporated at 300 V for 10 ms (unless otherwise specified) by square-wave single pulse using a Bio-Rad Gene Pulser (BioRad). Immediately after the electroporation ...
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bioRxiv - Bioengineering 2023Quote: ... was electroporated (pulsed twice using a square wave of 850 volts for 25 milliseconds) into BHK-21 cells using a Gene Pulser Xcell electroporator (BioRad); transfected cells were cultured in a T175 for 48h at 37 °C ...
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bioRxiv - Bioengineering 2024Quote: ... and polystyrene disposable cuvettes (Semi-Micro, 1 cm path, 1.5 mL, 12.5 mm square x 45 mm high; Bio-Rad, 2239955). The alginate and CaSO4 were mixed ...
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bioRxiv - Molecular Biology 2022Quote: ... and subjected to square wave electroporation using a single 20 millisecond pulse of 125 Volts (Gene Pulser Xcell Electroporator, Bio-Rad) [61] ...
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bioRxiv - Microbiology 2022Quote: ... 2×2 mm squares were cut out and placed into double-sided sticky 9×9 mm frame seals (Bio-Rad SLF0201) on a glass slide ...
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bioRxiv - Developmental Biology 2023Quote: ... an electrical pulse (single pulse square signal at 60 V for 20 ms) was immediately performed in a Gene pulser Xcell – CE module (Biorad®). Worms were left to rest for 2 h in the cuvette at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... These eggs were subjected square-wave electroporation (one pulse of 125 volts, 20 ms duration (Gene Pulser Xcell Electroporation System, Bio-Rad or the BTX ...
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bioRxiv - Microbiology 2021Quote: ... anti-CD11b-PE (Biorad, Hercules, CA, USA) to enumerate CD4+ T-cells and innate immune cells ...
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bioRxiv - Microbiology 2024Quote: ... followed by streptavidin-phycoerythrin (PE) (Bio-Rad). After three washes ...
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bioRxiv - Immunology 2024Quote: ... and F4/80-PE antibody (Bio-Rad) according to manufacturer instructions ...
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bioRxiv - Systems Biology 2021Quote: ... and the PDMS pad for medium perfusion was attached on the coverslip via a square-frame two-sided seal (Frame-Seal™ Incubation Chambers, Bio-rad). We immediately filled the device with the fresh medium and connected it to a syringe pump on the microscope stage.
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bioRxiv - Molecular Biology 2020Quote: ... The mixture was pipetted into a 4 mm pre-chilled electroporation cuvette and subjected to a square wave with a single 20 ms impulse at 125 v [79] (Gene Pulser Xcell Electroporator, Bio-Rad, USA), and subsequently maintained at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... and electroporated (square wave, 6 pulses, 30 V, 3 ms duration, 100 ms interval) using a gene Pulser XCell electroporator (Bio-Rad, 1652660). Zygotes were recovered and transferred to fresh KSOM drops and cultured overnight ...
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bioRxiv - Immunology 2022Quote: ... and mouse α-pig CD8β-PE (BioRad MCA5954PE). A panel to identify ileal ILCs (gating strategy shown in Figure 5A ...
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bioRxiv - Neuroscience 2020Quote: ... and PE-conjugated anti-CD21 mAb (Biorad, CA2.1D6)) ...
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bioRxiv - Immunology 2020Quote: ... and IgM-PE mAb (clone K52 1C3, BioRad Antibodies ...
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bioRxiv - Immunology 2021Quote: ... and IL-18 were measured on a Bio-Plex 200 instrument (Bio-Rad) using the Non-Human Primate Cytokine MILLIPLEX map 23-plex kit (Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... using 18S rRNA as housekeeping gene and CFX Manager software (Bio-Rad, USA).
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bioRxiv - Developmental Biology 2022Quote: ... using 18S rRNA as housekeeping gene and CFX Manager software (Bio-Rad, USA). RNA was sequenced paired by GENEWIZ company (Germany).
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bioRxiv - Bioengineering 2020Quote: ... Goat anti-rat PE (Biorad, STAR73, 1:10 dilution). All antibodies were diluted into PTxWH buffer ...
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bioRxiv - Immunology 2023Quote: ... CD172a (clone 74-22-15, PE-conjugated, Bio-Rad) and Fixable Viability Dye eFluor780 (ThermoFisher) ...
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bioRxiv - Immunology 2021Quote: ... onto a 10% Criterion TGX Precast Midi Protein Gel (18 well Bio-Rad, 5671034) in Nu PAGE MOPS SDS running buffer (Thermo Scientific NP0001) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Gels were then electroblotted at 0 °C in Towbin buffer (70) to PVDF membrane (Immun-Blot, Biorad) for 600 minutes at 30 V ...
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bioRxiv - Pathology 2022Quote: ... and PE- conjugated anti-IFNγ (MCA1783PE, clone CC302, Bio-rad). Cell acquisition was performed on the BD LSRFortessa™ Cell Analyzer (BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... and human 18S genes using the iQ™ SYBER® Green Supermix (Bio-Rad, #1708880) with the following conditions ...
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bioRxiv - Cell Biology 2022Quote: ... tubulin tyrosine ligase (TTL).(18) Cells were collected using 1x Laemelii Buffer (Bio-Rad, #1610737) containing protease (Milipore-Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... and proteins were loaded onto 18-well BioRad anyKD Criterion TGX gels (Bio-Rad, #5671124). For validation of HsNR4A3 protein knockout ...
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bioRxiv - Immunology 2021Quote: ... TCR expression was assessed by staining with NY-ESO 9V PE-conjugated tetramer (inhouse produced using refolded HLA*A02:01 with NY-ESO-1157-165 9V and streptavidin-PE [Bio-Rad AbD Serotec or BioLegend]) using flow cytometry.
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bioRxiv - Microbiology 2020Quote: ... using a 17-cm nonlinear pH 3.0-10.0 immobilized pH gradient (IPG) for plant proteins and a nonlinear pH 4.0-7.0 IPG strips (ReadyStrips, BioRad) for bacterial proteins in the first dimension ...
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bioRxiv - Plant Biology 2024Quote: ... the ORF was PCR amplified from Arabidopsis thaliana (ecotype Columbia-0) cDNA using iProof DNA polymerase (Bio-Rad), specific primers (ORF-MAP3K19-F ...
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bioRxiv - Microbiology 2021Quote: ... 18S V4+V5 amplification was achieved using the iProof DNA polymerase (Bio-Rad Laboratories, Hercules, CA) with primers V4-1 and V4-4 as previously described13 ...
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bioRxiv - Immunology 2022Quote: ... subgenomic RNA and mice 18s rRNA has then been amplified using iQ SYBR green supermix (Biorad) and performed using CFX96 real time system (Biorad) ...
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bioRxiv - Molecular Biology 2023Quote: ... at 18 V for 1 h using Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad). Membranes were blocked in 5% skimmed milk in tris-buffer saline (TBS ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were loaded on 10% 18-well Stain free SDS-gel (Bio-Rad Laboratories, Hercules, USA) for electrophoresis ...
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bioRxiv - Molecular Biology 2021Quote: ... PE gels were visualized by phosphorimaging (GE Healthcare or Bio-Rad) and quantified by ImageQuant 5.1 (GE ...
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bioRxiv - Biochemistry 2020Quote: ... we used commercially available BiCatcher-HRP or BiCatcher-PE (Bio-Rad).
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bioRxiv - Plant Biology 2024Quote: The AtGCN5 coding sequence amplified from Arabidopsis Col-0 cDNA using iProof™ High-Fidelity PCR Kit (Bio-Rad) was inserted into pDONR221 vector (Thermo Fisher Scientific ...