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Citations for Bio-Rad :
4851 - 4900 of 7138 citations for 7 Chloro 4 hydroxy 1 8 naphthyridine 3 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... secondary antibodies were incubated with the PVDF membrane for 1 hour at room temperature (Goat anti-rabbit antibody, BioRad, 1:3000). Then ...
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bioRxiv - Cancer Biology 2019Quote: ... transferred onto PVDF membrane and probed with primary antibody (Table 1) at a dilution of 1:1000 prepared in 5% blotting grade blocker (Bio-Rad) for 12-16 h at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... One-third of the PCR volume was visualized on a 1% agarose gel run in 1 × TAE and imaged using a ChemiDoc imager (Bio-Rad). We validated each amplicon by direct sequencing at The Centre for Applied Genomics (The Hospital for Sick Children ...
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bioRxiv - Microbiology 2021Quote: ... The solution was then pelleted to remove cell debris and the supernatant was mixed 1:1 with 2x Laemli sample buffer (Bio-Rad) and beta-mercaptoethanol ...
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bioRxiv - Microbiology 2020Quote: ... was added to each well and samples were heated from 25 to 95 °C in 1 °C steps of 1 min each in a CFX96 Touch™ Real-Time PCR Detection System (BioRad). Excitation/emission filters of 492 and 516 nm were used to monitor the fluorescence increase resulting from binding of the Sypro Orange to exposed hydrophobic regions of the unfolding MsSepFcore ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were heated from 20 to 95 °C at the rate of 1 °C min-1 and fluorescence signals were monitored using quantitative real-time PCR (CFX96 Real-Time System, Bio-Rad). Each experiment was done in triplicate and the average values were taken for analysis ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were transferred to a PDVF membrane for 1 hour at 100V and blocked for 1 hour (5% blotting-grade blocker (Bio-Rad) in PBS containing 0.05% Tween 20) ...
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bioRxiv - Microbiology 2022Quote: ... The working solutions of the compounds and DNA hairpins/duplexes were mixed (1:1 ratio, 25 μL of each solution) in the wells of a 96 well plate (Bio-Rad). The wells were covered and placed in a DNA Engine Opticon system for melting ...
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bioRxiv - Cell Biology 2022Quote: Images of THP-1 wt and THP-1 RTN1A+ cells in cultures were taken with the ZOE fluorescence cell imager (Bio-Rad). and the numbers of cellular aggregates per 0.7 mm2 from 4 FOV have been quantified using ImageJ Fiji (Fig 5G) ...
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bioRxiv - Molecular Biology 2019Quote: ... the membrane was incubated overnight at 4°C with primary antibodies diluted 1:1000 followed by a further 1 h incubation in the corresponding horseradish peroxidase conjugated secondary antibody (Bio-Rad) at a 1:10000 dilution ...
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bioRxiv - Neuroscience 2022Quote: Tissue sections stained by mRNA FISH and fixed primary neurons were washed with 1 x PBS and blocked in 1 x PBS supplemented with 5 % (vol/vol) goat serum (Bio-Rad), 1 % (wt/vol ...
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bioRxiv - Neuroscience 2020Quote: ... 1:5000), or donkey anti-goat antibody (sc-2020, 1:5000) and signal was detected with enhanced chemiluminescence substrate (Bio-Rad).
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bioRxiv - Cell Biology 2019Quote: ... stain-free image was acquired then blot was incubated in 1:1 mixture of Clarity Western ECL Substrate reagents (Bio-Rad). Blots were then imaged on ChemiDoc MP Imaging System and analyzed with Bio-Rad Image Lab software (v5.2.1).
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bioRxiv - Zoology 2020Quote: ... Samples were incubated in a 1:1 (w/v) ratio of 2× sample buffer (0.2M DTT, BioRad Laemmli Sample Buffer #1610737) at room temperature for 20 minutes ...
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bioRxiv - Microbiology 2021Quote: ... HIV-1 copy number was determined by a total HIV-1 DNA assay on droplet digital PCR (Bio-Rad, QX200 system), as described previously (Rutsaert et al. ...
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bioRxiv - Immunology 2020Quote: ... 1:400) to identify αβT cells and mouse anti-pig SWC5 (clone b37c10, Bio-Rad AbD Serotec, Puchheim, Germany, 1:100) for identification of γδT cell subpopulation ...
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bioRxiv - Biochemistry 2020Quote: ... and pellet dissolved in the original sample volume of 1:1 PBS: SDS loading buffer were then analyzed by SDS-PAGE (Bio-Rad) at 160 V for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Chemiluminescence was detected by adding Clarity Western ECL Substrate (1:1 of luminol and peroxide solutions) in a ChemiDoc Touch Imaging System (Bio-Rad).
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bioRxiv - Biochemistry 2022Quote: Proteins were reduced with 1 mM TCEP for 1 h then passed through a 10DG desalting column (Bio-Rad, Hercules, CA) to remove the TCEP ...
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bioRxiv - Microbiology 2022Quote: ... His6 tagged proteins were detected with anti-His6 primary antibody (Pierce; 1:6,000) and anti-mouse secondary antibody (Biorad; 1:10,000). GroEL was detected with anti-GroEL primary antibody (Pierce ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked in 5% milk and incubated with anti-GFP (1:1000) followed by Amersham ECL goat anti-rabbit horseradish peroxidase conjugate 1:5000 (Bio-Rad). Blots were developed using chemiluminescence (Super Signal West Femto ...
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bioRxiv - Biophysics 2022Quote: ... Both this sample and the total cell protein sample were mixed 1:1 with 2x loading buffer: 50% 4x Laemmli Sample Buffer (Bio-Rad), 30% 100 mM DTT ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated at RT for 1 h with HRP-linked secondary antibodies (Bio-Rad 170-6515 and 172-1011, 1:5000), imaged via chemiluminescence on a ChemiDoc Imaging System (Bio-Rad) ...
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bioRxiv - Immunology 2024Quote: ... tissue sections were blocked in 10% normal goat serum for 1 h at room temperature and incubated in rat anti-mouse CD4 (1:200; MCA2691; Bio-Rad) or CD8a (Biolegend ...
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bioRxiv - Microbiology 2023Quote: ... was added to each well and the mixture was heated from 25 to 95°C in 1°C steps of 1 min each in a CFX96 Touch™ Real-Time PCR Detection System (BioRad). Excitation and emission filters of 492 and 516 nm respectively ...
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bioRxiv - Developmental Biology 2023Quote: ... The secondary incubation was performed with goat anti-rabbit IgG-HRP conjugate (1:3000) or goat anti-mouse IgG-HRP conjugate (1:3000) (Bio-Rad) for 1 hr at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... probed with a goat anti-mouse or goat anti-rabbit IgG H+ L-HRP conjugated secondary antibody (1:5000-1:10000) (Bio-Rad) for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: Pellets of 1×106 treated cells were incorporated in 1% agarose at 50°C and cast into molds (Biorad, Grabels, France) avoiding air bubbles ...
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bioRxiv - Microbiology 2024Quote: ... for 1 hour RT followed by 1:2000 IRDye800 conjugated goat-anti-rabbit antibody (Li-COR Biosciences) or 1:1000 goat-anti-rabbit HRPO (Bio-Rad) for 1 hour at RT ...
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bioRxiv - Neuroscience 2021Quote: ... blocked in 10% normal donkey serum, and then stained in antibodies overnight (rabbit anti-Iba1, 1:500, Wako and CD68 (Biorad 1:100)) ...
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bioRxiv - Genomics 2021Quote: ... at 4°C in 1×TAE buffer (40 mM Tris-acetate, 1 mM EDTA) using 0.55% Certified Megabase agarose gel (Bio-Rad, 161-3109). Short-range PFGE ran at 4.2 or 4.5 V/cm with a pulse time from 40 to 70 s for 24 h ...
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bioRxiv - Neuroscience 2021Quote: ... We obtained immunofluorescence labelling of microglia using an Iba-1 antibody (Wako, Richmond, USA) with a dilution of 1:200 co-stained with CD68 (BioRad, California, USA) with a dilution of 1:100 ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was then incubated for 1 h at RT with goat anti-Lipid A LPS antibodies (diluted 1:1,000 in blocking buffer; Bio-Rad; Cat # OBT1844). Blots were washed three times with TBST (50 mM Tris-Cl ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were probed for 1 hour at room temperature with 1:500 anti-XLF(New England Peptides, details in Methods under Immunodepletion) or 1:1000 anti-His (Bio-Rad MCA1396A) in PBST with 2.5% BSA ...
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bioRxiv - Cancer Biology 2019Quote: ... Lysates were precleared with control antibody plus 30 µL of 1:1 Protein-A/G magnetic beads (BIO-RAD, Hercules, CA, USA) for 1 h at 40C ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked in 5% dry non-fat milk in PBST and probed using PRP8a antibody (1:500) and an HRP coupled anti-Rabbit secondary antibody (Biorad, 1:10000). All antibodies were diluted in 1% dry nonfat milk in PBST ...
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bioRxiv - Microbiology 2019Quote: ... pH 8 for 1 h at 37°C and then loaded into the wells of 1% pulse-field certified agarose gel (Bio-Rad Laboratories) in 0.5X TBE (45mM Tris HCl ...
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bioRxiv - Bioengineering 2020Quote: ... and annealed from 90°C to 20°C at 1 °C min-1 in a T100™ Thermal Cycler (Bio-Rad, USA). Following construction ...
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bioRxiv - Cancer Biology 2022Quote: ... membranes were incubated with complementary HRP conjugated secondary antibody (sheep anti-mouse IgG-HRP, 1:10000, GE Healthcare, NA931; goat anti-rabbit IgG-HRP, 1:5000, Bio-Rad, 1706515 ...
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bioRxiv - Developmental Biology 2023Quote: ... All cDNA samples were diluted 1:5 and 1 μL was used in quantitative PCR (qPCR) reactions with iTaq Universal SYBR Green Supermix (Bio-Rad, #1725124), according to manufacturer’s instruction and using the Applied Biosystems QuantStudio 7 flex qPCR System ...
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bioRxiv - Molecular Biology 2023Quote: ... The HIV-1 copy number was determined by a total HIV-1 DNA assay on droplet digital PCR (Bio-Rad, QX200 system), as described previously (28) ...
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bioRxiv - Immunology 2023Quote: ... The samples and a protein ladder (a 1:1 mix of All Blue Prestained Protein Standards: Unstained Protein Standards; Precision Plus Protein™, Bio-Rad) were loaded to 4-15% mini-PROTEAN™ TGX Stain-Free™ Protein Gels (Bio-Rad) ...
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bioRxiv - Biochemistry 2022Quote: ... A 1:1 Langmuir binding model was used to fit the data measured on a ProteOn™ XPR36 instrument (Bio-Rad Laboratories) using ProteOn™ Manager Software (Version 3.1.0.6 ...
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bioRxiv - Cell Biology 2023Quote: 600 μL of DMEM GlutaMax™ supplemented with 10% FBS, 20 μg/mL gentamycin, and either 100 ng/mL recombinant mouse Monocyte chemoattractant protein-1 (MCP-1, CCL2) (Bio-Rad, PMP35) or vehicle control (ddH2O) ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was incubated with secondary antibodies for 1 h at room temperature: goat anti-rabbit IgG (H+L)-HRP conjugate (170-6510, Biorad, 1:10000) and goat anti-mouse IgG (H+L)-HRP conjugate (170-6516 ...
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bioRxiv - Microbiology 2024Quote: ... SigA proteins were detected using anti-SigA85 (1:10,000 dilution) combined with anti-rabbit-HRP secondary (Bio-Rad, #1706515, 1:5,000 dilution) antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... molten 1% certified low melt agarose (Bio-Rad, 1613112) in PBS was equilibrated to 45°C ...
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bioRxiv - Cell Biology 2020Quote: ... followed by instillation of 1% low melting agarose (BioRad) through the trachea to prevent leakage of dispase ...
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bioRxiv - Cell Biology 2020Quote: ... Sheep anti-TGN46 from Biorad (AHP500, Immunofluorescence 1:500), Hoechst 33342 from Invitrogen Molecular Probes (H3570).
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bioRxiv - Immunology 2021Quote: ... CD68 (1:200; Bio-Rad, cat no. MCA1957, monoclonal), and C1q (1:200 ...