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Citations for Bio-Rad :
4851 - 4900 of 7899 citations for 6 Quinoxalinecarboxaldehyde 1 2 3 4 tetrahydro 1 methyl 3 oxo 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... These extracts were loaded onto a 4-20% SDS PAGE gel (Bio-Rad, Hercules, California, USA), transferred onto a nitrocellulose membrane and identified with a rabbit anti-target antibody (see Table E2) ...
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bioRxiv - Neuroscience 2024Quote: ... at 11 V for 14 hours at 4° C using the BioRad Criterion Blotter (BioRad, #1704070). Membranes (BioRad ...
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bioRxiv - Neuroscience 2024Quote: ... at 100 V for 30 minutes at 4° C in Tris/Glycine buffer (Bio-Rad, 1610734) with 20% methanol ...
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bioRxiv - Neuroscience 2024Quote: ... and were run on a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad). Proteins were transferred on a nitrocellulose membrane and blocked for two hours at room temperature in blocking solution and incubated in primary antibody solution for 48 hours at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... 4-15% or 10% Mini-PROTEAN TGX Stain-Free Precast Gels (BioRad, 4568123, 4568083 or 4568033). Gels were activated by exposure to UV light for 45 seconds using a Chemidoc (BioRad ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal amounts of total protein were loaded into a 4-15% gradient gel (Bio-Rad Laboratories). Proteins were then transferred to a nitrocellulose membrane (Bio-Rad Laboratories) ...
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bioRxiv - Cell Biology 2024Quote: ... 25 µg of total protein was separated on 4-20 % precast SDS-PAGE gels (Bio-Rad). Depending on the protein size ...
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bioRxiv - Cell Biology 2024Quote: ... ∼47 μg of protein was separated on a 4%-15% gradient SDS-polyacrylamide gel (Bio-Rad) which were transferred to nitrocellulose membranes ...
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bioRxiv - Microbiology 2024Quote: ... All samples were run on 4– 20% Criterion TGX Stain-Free Protein Gels (Bio-Rad Laboratories). After electrophoresis ...
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bioRxiv - Immunology 2024Quote: ... The samples were analyzed on 4-12% criterion XT Bis-Tris polyacrylamide gel (Bio-Rad #3450124) electrophoresis followed by electrotransfer onto PVDF membrane and were probed using an antibody against CD63 (1:500 ...
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bioRxiv - Microbiology 2024Quote: ... Hydrolysis products were separated using a self-packed column of P-4 fine resin (Bio-Rad) and identified using the neutral sugar assay ...
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bioRxiv - Microbiology 2024Quote: ... 10μL of each protein sample was electrophoresed on a 4 - 20% gradient polyacrylamide gel (Bio-Rad) in Tris-glycine running buffer (25 mM Tris base ...
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bioRxiv - Microbiology 2024Quote: ... 10 µl purified LPS was loaded and separated on a 4-12% gradient Tris-glycine (BioRad) using SDS-PAGE and visualized by Western blotting or with the Pro-Q emerald lipopolysaccharide stain kit (Thermo Fischer ...
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bioRxiv - Immunology 2024Quote: ... Lysate (40-50 µg) was loaded into 4-15% Mini-PROTEAN® TGX gels (Bio-Rad) and separated by SDS-PAGE electrophoresis ...
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bioRxiv - Microbiology 2024Quote: ... a double PARAFILM (Sigma)-layer flow chamber was incubated at 4°C overnight with 25ng/μL Digoxigenin Antibody (Bio-Rad) that adsorbed onto the polystyrene-covered lower surface ...
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bioRxiv - Molecular Biology 2024Quote: ... were electrophoresed on 4-20% Mini-PROTEAN® TGX Stain-Free gels (Bio-rad, Cat#4568096) and then transferred onto a polyvinylidene fluoride (PVDF ...
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bioRxiv - Immunology 2024Quote: ... and separated by electrophoresis on precast 4%–15% polyacrylamide gradient gels (Bio-Rad Laboratories, Herculues, CA). Separated proteins were transferred to nitrocellulose membranes and then blocked overnight at 4°C in Western blotting block buffer (TBS-Tween (0.5 % ...
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bioRxiv - Systems Biology 2024Quote: ... Equivalent amounts of protein were loaded on 4-15% Mini-PROTEAN precast TGX gel (Bio-Rad). After electrophoresis ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Total protein was separated on 10% SDS-PAGE reducing gels or 4%-20% detergent gels (BioRad). The resolved proteins were transferred onto a PVDF membrane using the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... Lysates were run on 4–15% Mini-PROTEAN® TGX™ Precast Gels (BIO-RAD 4561083) and blots were probed with anti-LBR (1:1000 ...
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bioRxiv - Bioengineering 2024Quote: ... The mixture was transferred to a pre-cooled (4°C) 0.4mm electroporator cuvette (BioRad, Hercules, CA). Electroporation was performed on the BioRad GenePulser XCell with the following protocol parameters ...
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bioRxiv - Neuroscience 2021Quote: ... Each sample was prepared for western blotting by diluting 20 μg of protein with 1 × Laemmli buffer (Bio-Rad #1610747) and ultrapure water (Invitrogen #10977015 ...
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bioRxiv - Biophysics 2020Quote: The prepared DNA samples (5 μL) were mixed thoroughly with 1 μL of 6X DNA loading buffer (Bio-Rad Laboratories). The mixtures were loaded into the wells of the prepared gel ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell viability was determined at indicated time points by adding 1/10 volume AlamarBlue solution (Biorad, Hercules, CA, United States) to each well ...
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bioRxiv - Cell Biology 2020Quote: ... and equal amounts (1–2µg) were used for reverse transcriptase reaction using an iScript™ Reverse Transcription Supermix (Bio-Rad). qPCR was run using the SYBR® Green Master Mix on a CFX Connect™ Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were co-encapsulated with Drop-Seq beads (ChemGenes: lot 083117) in 1 nL droplets using 15000 mL/hr oil (QX200, Biorad), 4000 mL/hr cell suspension and 4000 mL/hr bead flow rates (droplet generation frequency 2 kHz) ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amount of proteins was loaded onto 12% SDS-PAGE gel (acrylamide: bisacrylamide 29:1, cat# 161-0146, Bio-Rad) and after size separation blotted onto 0.2 μm nitrocellulose membrane (cat# 162-0112 ...
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bioRxiv - Cell Biology 2020Quote: ... incubating with HRP conjugated secondary antibodies (1:5000) and developing with Clarity™ Western ECL blotting substrate (Bio-Rad, 1705061) and imaging with X-ray film ...
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bioRxiv - Genetics 2021Quote: ... Proteins were visualized using horseradish peroxidase-conjugated goat anti-rabbit secondary antibody conjugate (1:2000 dilution, Bio-Rad laboratories, #1706515) or horseradish peroxidase-conjugated goat anti-mouse secondary antibody conjugate (1:2000 dilution ...
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bioRxiv - Microbiology 2021Quote: ... and switch time linearly ramped from 1 s to 5 s over 14-15 h (CHEF DR III, Bio-Rad). DNA bands were visualized by staining the gel with a solution of 0.5 µg/mL ethidium bromide ...
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bioRxiv - Developmental Biology 2022Quote: ... membranes were incubated with ECL Western Blotting Substrate (Thermo, 32106) for 1 minute prior to imaging with the ChemiDoc system (BioRad).
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of total RNA was used for reverse transcription with iScript™ cDNA synthesis kit (Bio-Rad, Hercules, CA) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 1 µg of plasmid was added to electrocompetent GBS cells and transferred to a cold 1 mm cuvette (Fisher or BioRad). Electroporation was carried out at 2.5 kV on an Eppendorf eporator ...
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bioRxiv - Neuroscience 2021Quote: ... 1 µg of cleaned RNA was used to generate cDNA with iScript reverse transcriptase per the manufacturer’s protocol (Bio-Rad). 20 µL quantitative real-time PCR reactions with 100 ng of cDNA input were carried out using TaqMan Fast Advance Master Mix (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-IBA-1 antibody (0.4 μg/mL, FUJIFILM Wako) and rat anti-CD68 antibody (5 μg/mL, clone FA-11, Bio-Rad) was used ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesized from 1 μg of total RNA with random primers using an iScript cDNA synthesis kit (Bio-Rad) and analyzed with a Bio-Rad CFX96 real time system ...
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bioRxiv - Immunology 2022Quote: ... 1-0.2 μg of RNA was used to synthesize cDNA using the iScript cDNA synthesis kit (Bio-Rad, Hercules, CA). The cDNA template was mixed with iTAQ SYBR green universal super mix (Bio-Rad) ...
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bioRxiv - Developmental Biology 2020Quote: ... membranes were rinsed several times in 1xTBSTw and incubated with goat anti-rabbit HRP-conjugated secondary antibody (1:20,000; Bio-Rad, catalog 179-5046 ...
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bioRxiv - Molecular Biology 2019Quote: ... and goat anti-rabbit IgG H&L HRP conjugate at a dilution of 1:2000 (Bio-Rad, Hercules, CA, USA) was used as the secondary antibody ...
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bioRxiv - Cell Biology 2019Quote: ... the blots were incubated with a secondary antibody conjugated to horseradish peroxidase (HRP) (1:5,000, Jackson Immuno Research Laboratories) and detected with a ChemiDoc XRS system (Bio-Rad). Primary antibodies used in western blot are listed below ...
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Evolutionary repair: changes in multiple functional modules allow meiotic cohesin to support mitosisbioRxiv - Evolutionary Biology 2019Quote: ... immunoprecipitated chromatin and a 1:100 dilution of the input chromatin were separately recovered by boiling with a 10% Chelex-100 resin (BioRad) before treating with 25 μg/ml Proteinase K at 55 °C for 30 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... membrane was washed repeatedly with PBS-T and incubated 1 h RT with the secondary antibody in milk PBS-T (Goat anti-mouse IgG HRP conjugate (170-6516; BioRad); Goat anti-rabbit IgG HRP conjugate (170-6515 ...
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bioRxiv - Pathology 2019Quote: ... Bands corresponding to EPCR and the housekeeping protein β-actin (mouse IgG #NB600-501, Novus, at 1:40,000) were detected by the chemiluminescence method (Clarity Western ECL Bio-Rad) and finally densitometrically measured by ImageJ 1.6.0 software.
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bioRxiv - Cell Biology 2019Quote: ... The secondary antibodies used for Western blotting were goat ⍰ -mouse IgG HRP conjugate (170-6516 from Bio-Rad; 1:2000) and goat ⍰ -rabbit IgG HRP conjugate (170-6515 from Bio-Rad ...
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bioRxiv - Cancer Biology 2019Quote: ... according to the manufacture’s protocol and 1 µg RNA was used to convert to cDNA using iScript Select cDNA Synthesis Kit (Bio-Rad) in the presence of both oligo (dT ...
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bioRxiv - Cell Biology 2020Quote: ... were incubated with NaIO4 for 1 h then 15 min with NaS2O3 and mixed with 100 μl AffiGel Hz Hydrazide Gel (Biorad) overnight ...
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bioRxiv - Neuroscience 2019Quote: ... Reverse transcription of 1 μg of total RNA per sample was performed using the iScript cDNA synthesis kit (Bio-RAD). Transcript levels were determined by quantitative real-time RT-PCR as previously reported [14] using primers provided in Supplementary Table 1 and normalizing expression to ATCB transcript levels.
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bioRxiv - Microbiology 2020Quote: ... The gel was run for 1 h at 100 V and visualized on a ChemiDoc MP Imaging System (BioRad, Germany).
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bioRxiv - Neuroscience 2019Quote: ... Each qPCR reaction was performed in triplicate using specific primers (Supplementary Table 1) and iQ SYBR Green Supermix (Bio-Rad) in a CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Synthetic Biology 2019Quote: ... Primary antibodies used for immunolabelling (Table 1) were utilized in combination with appropriate secondary antibodies purchased from BioRad (München, Germany) and BCIP-NBT (VWR international ...