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Citations for Bio-Rad :
4701 - 4750 of 6638 citations for 10 14 Cadinene 4 5 Diol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Real-time PCR was performed on 5 ng/μL cDNA using iTaq Universal SYBR Green Supermix (Bio-rad, 64361172) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Onion epidermal cells were bombarded with 5 μg of each DNA construct using a helium biolistic gun (BIO-RAD). Treated epidermal cells were kept in the dark at room temperature for 16 h before analysis by confocal microscopy (Confocal Olympus FV 1000) ...
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bioRxiv - Microbiology 2020Quote: ... purified on Ni-NTA resin (G-Biosciences, #786-940 in 5 ml Bio-Scale™ Mini Cartridges, BioRad #7324661), then dialyzed ...
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bioRxiv - Neuroscience 2020Quote: ... and blocked with 5% (w/v) non-fat milk tris-buffered saline (TBS) with 0.1% Tween-20 (Bio-Rad) for 1 hour at room temperature ...
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bioRxiv - Synthetic Biology 2020Quote: ... Genomic DNA was obtained from single adults via incubation in 100µL of 5% w/v Chelex beads (BioRad Inc.) at 55°C ...
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bioRxiv - Microbiology 2020Quote: ... 20 mL blocking buffer supplemented with 5 μL of horseradish peroxidase (HRP) conjugated rabbit anti-mouse secondary antibody (BioRad) was added and incubated for 2 hours at room temperature with agitation ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and 15μg protein in 5x Laemmli buffer with 5% b-mercaptoethanol was loaded on Mini-PROTEAN TGX Precast Gels (BioRad). Gel lanes were cut into three sections for peptide extraction ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were washed 3 times with PBS and then blocked for 1 hour at room temperature with DPBS with 0.4% Triton X-100 and 5% non-fat dry milk (BioRad). Cells were stained with primary and secondary antibodies (Table S3 ...
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bioRxiv - Cell Biology 2020Quote: Immunoprecipitated proteins were resuspended in SDS-PAGE sample buffer and heated 5 minutes at 95°C before loading onto a 7.5% acrylamide mini-Protean TGX gel (BioRad). After 7 minutes of electrophoresis at 100 V the gel was stained with Imperial Protein stain (Thermo ...
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bioRxiv - Systems Biology 2020Quote: ... A total of 800 µl of cell suspension mixed with 10 µg of pDM304_Red-FL2 in a 4-mm-width cuvette was subjected to electroporation (two 5 sec separated pulses with 1.0 kV and a 1.0 msec time constant) using a MicroPulser (Bio-Rad). These cells were plated on 4–6 pieces of 90-mm plastic dishes with HL5 medium and incubated at 22°C for 18 hours under the non-selective conditions and then cultured in the presence of 10 µg/ml G418 (Wako) ...
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bioRxiv - Genomics 2021Quote: ... 1 μL diluted cDNA in a total volume of 5 μL using a CFX384 Real-Time System (Bio-Rad). For each sample we conducted three technical replicates and used their mean as the data point ...
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bioRxiv - Genomics 2021Quote: ... They were then boiled for 5 minutes at 99°C after the addition of Laemmli buffer (Cat. #1410737, Biorad). Proteins were separated by SDS-PAGE and transferred to a membrane with a Trans Blot TURBO (Biorad) ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μM of proteins and 3x of SYPRO™ Orange were pipetted into a 96-well PCR plate (BioRad, MicroAmp Fast 96-Well Reaction plate ...
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bioRxiv - Biochemistry 2021Quote: Nucleosome quality was analyzed by running the nucleosome on native PAGE on a 5% TBE gel in 0.5X TBE buffer (BioRad) that was run for 60 min at 150 volts ...
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bioRxiv - Biochemistry 2021Quote: ... was added to a final concentration of 1X to the quenched reaction and samples were loaded onto a 5% TBE gel and run for 60 min at 150 volts in 0.5X TBE Buffer (BioRad). Gels were stained with ethidium bromide and imaged on a BioRad ChemiDoc ...
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bioRxiv - Immunology 2022Quote: ... Internal loading control was performed by stain free visualization after 5 min activation on a ChemiDoc imaging system (BioRad). Protein transfer was done either on nitrocellulose membrane or polyvinylidene fluoride (PVDF ...
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bioRxiv - Neuroscience 2022Quote: ... 5% β-mercaptoethanol) to be run on SDS-PAGE gels using the Mini Protean system (Bio-Rad, no. 1658004). Samples were heated at 95°C for 5 minutes and loaded on 4-15% TGX gels (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... the membrane was blocked using 5% non-fat milk in 0.1% TBS-T (1X Tris-Buffered Saline, 0.1% Tween 20, Bio-Rad) for 1 hr at RT ...
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bioRxiv - Microbiology 2022Quote: ... samples were loaded onto Mini-PROTEAN TGX Pre-cast Stain-Free gels (5-15%, BioRad Laboratories, Hercules, CA, USA) and total protein was visualised post-transfer to PVDF membranes on ChemiDoc Touch Imaging System ...
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bioRxiv - Microbiology 2022Quote: ... Each reaction consisted of 5 μl of extracted DNA and 15 μL of iTaq™ Universal Probes Supermix (BioRad), containing 0.4 mM of each primer ...
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bioRxiv - Microbiology 2024Quote: ... and then lysed by a 5-minute incubation with with 50 uL iScript RT-qPCR Sample Preparation Reagent (BioRad) (64) ...
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bioRxiv - Immunology 2024Quote: ... The indicated mass of protein was boiled for 5 minutes at 95°C with 4x Laemmeli Buffer (BioRad #1610747) supplemented with 2.5% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: 0.2 – 1 mL (sufficient to cover the sample) of 5% Chelex 100 Resin (Bio-Rad Laboratories, Hercules, CA, USA) with 0.2% Tween20 and 0.1 mg/mL proteinase K (Merck ...
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bioRxiv - Neuroscience 2024Quote: ... After 1h-incubation with HRP-conjugated secondary antibodies diluted in 5% milk in TBST (anti-mouse HRP (BioRad, 1706516) 1:10000 ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked with 5% non-fat milk in PBS-T (0.5% Tween-20 [1706531, Bio-Rad] in PBS) for 1 hour ...
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bioRxiv - Cell Biology 2024Quote: ... rinsed twice with water and resuspended in 20 µL Laemmli sample buffer (Bio-Rad, 2X with 5% β-mercaptoethanol). After boiling for 5 min at 100°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins in cell lysates were heated at 95[ for 5 minutes in Laemmli samples buffer (Bio-Rad, Hercules, CA), resolved by 10% SDS-PAGE ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were loaded onto polyacrylamide native gels (5% polyacrylamide 29:1 [BioRad], 1× TBE, 0.3% APS, and 0.06% Temed) and protein/DNA complexes were resolved by electrophoresis at 60 V for 2 h at 24 °C in 1X TBE ...
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bioRxiv - Biophysics 2024Quote: ... Blots were washed 3 times with TBST for 5 min and visualized using a ChemiDoc MP Imaging System (Biorad).
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bioRxiv - Biochemistry 2024Quote: ... The enzyme was purified using a 5-mL Ni-charged EconoFit Nuvia IMAC Column (Bio-Rad, Hercules, CA, USA) and a linear gradient of 10 - 250 mM imidazole in binding buffer (100 mL in total at 2.5 mL/min flow rate) ...
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bioRxiv - Cell Biology 2024Quote: ... and heated to 98°C for 5 min and transferred to Trans-Blot PVDF Transfer membranes (Bio-Rad, #1704157). Membranes were blocked for 1 h at room temperature with TBS-T (0.1% Tween 20 ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were heated to 95°C with 2X sample buffer for 5 mins before loading the samples directly to a 0.2μm nitrocellulose (BioRad, #1704159) membrane ...
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bioRxiv - Microbiology 2023Quote: ... both proteins were supplemented with 5 mM MgCl2 and protein concentration was determined using a colorimetric assay (Bio-Rad). Proteins were then mixed at a 2.5:1 mass ratio of KtrC to N-terminal Strep-tagged KtrA (11 mg of KtrC and 4.5 mg of N-terminal Strep-tagged KtrA in 10 mL total volume of 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... pH 7.5] with 0.1% (v/v) Tween 20) containing 5% Blotting-Grade Blocker (Bio-Rad Laboratories, cat. #170-6404) for 30 mins ...
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bioRxiv - Genetics 2023Quote: ... The membrane was blocked 1 hr with 5% Blotting Grade Blocker Non-Fat Dry Milk (Bio-Rad, Cat# 1706404XTU) and incubated overnight with the primary antibodies [TTC3 Polyclonal Antibody (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was washed four times in TBS-T for 5 min each before incubation for 1 h with secondary antibody (1:2,000 dilution of BioRad affinity purified goat α-rabbit IgG conjugated to alkaline phosphatase ...
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bioRxiv - Plant Biology 2023Quote: ... Leaf lysates were cleared by centrifugation at 10,000×g for 5 min and total protein normalized via Bradford Assay (Biorad). Extracts were prepared for SDS-PAGE with a 5x loading buffer containing 10% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... To visualize the protein bands, membranes were incubated (5 min, RT) in Clarity™ western ECL substrate (Bio-Rad) and imaged using the ChemiDoc™ MP imaging system (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... The transferred membrane was briefly washed with TBST (0.1% Tween-20 in TBS) and blocked with Blocking Buffer (5% Blotting-Grade Blocker [1706404, BioRad] or 5% BSA [A9647-50G ...
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bioRxiv - Bioengineering 2023Quote: ... cDNA was diluted 1:5 and RT-qPCR was done using SsoAdvanced™ Universal SYBR Green Supermix (Bio-Rad) with PrimePCR Assays (CCDC40 ...
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bioRxiv - Plant Biology 2023Quote: ... at 1:10,000 dilution in 5% milk PBST and ECL prime chemiluminescent reagent (Amersham: RPN2236) on a ChemiDoc gel imager (Biorad).
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of protein samples (5-20µg) were diluted in RIPA buffer and βME-Laemmli buffer (Bio-Rad Laboratories) to equal volumes and boiled for 7 minutes at 95°C ...
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bioRxiv - Biochemistry 2023Quote: ... The soluble fraction was loaded onto a 5 mL Bio-Scale Mini Nuvia IMAC Ni-charged Cartridges (Bio-Rad) which was pre-equilibrated with 5 column volumes (CV ...
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bioRxiv - Neuroscience 2023Quote: ... Protein extracts were denatured 5 min at 95°C in a Laemmli buffer (Bio-Rad, Marnes-la-Coquette, France) with β-mercaptoethanol (1/10 ...
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bioRxiv - Molecular Biology 2024Quote: ... whereafter 5 μl was used for a 25 μl qPCR reaction using 2x SYBR Green Master Mix (Bio-Rad) in a CFX96 Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... which were boiled at 95°C for 5 minutes before loading in pre-cast PAA gels (Bio-RAD, 4561084) and run at 100V for approx ...
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bioRxiv - Microbiology 2024Quote: ... pH 7.5] with 0.1% (v/v) Tween 20) containing 5% Blotting-Grade Blocker (Bio-Rad Laboratories, cat. #170-6404) for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... we incubated the samples 5 min at 65°C in a C1000 thermal cycler (Bio-Rad, Hercules, CA, USA). We added a synthesis mixture of 10 mM deoxynucleoside triphosphates ...
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bioRxiv - Molecular Biology 2024Quote: ... The lysate was clarified and incubated at 55°C for 5 minutes with Laemmli sample buffer (1610747, Bio-Rad). Samples were run on a 4–15% Mini-PROTEAN™ TGX Stain-Free™ Protein Gel (4568086 ...
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bioRxiv - Neuroscience 2024Quote: ... membranes were blocked for 1 hour with 5% (w/v) skimmed milk powder in TBS-Tween 20 (Bio-Rad) (TBS-T ...