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Citations for Bio-Rad :
4601 - 4650 of 5185 citations for Mouse CD252 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... Transfer was done into PVDF membranes using a Trans-Blot Turbo Transfer System and Trans-Blot Turbo RTA Transfer Kit (BioRad, #1704273). After 1 h of blocking in 5% milk ...
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bioRxiv - Neuroscience 2020Quote: ... Five hundred ng of purified RNA were used for cDNA synthesis using an iScript cDNA synthesis kit (Bio-Rad, Irvine, CA), and real-time quantitative PCR (qPCR ...
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bioRxiv - Bioengineering 2021Quote: ... A preamplification step that amplifies the amount of cDNA available for downstream RT-qPCR analysis was conducted using SsoAdvanced PreAmp Kit (BioRad, 1725160). RT-qPCR was performed using TaqMan Gene expression assays (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Cell lysates were boiled for 10 min and quantified with the DC protein assay kit following the microplate protocol provided by the company (Bio-Rad). Whole cell lysates (15-40μg protein ...
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bioRxiv - Biochemistry 2021Quote: ... RQN > 9) were prepared using the NEBNext Ultra II Directional RNA Library Kit for Illumina with NEBNext rRNA depletion (Bio-Rad). Protocols were followed as per the manufacturer’s guidelines except that 40X adaptor dilutions were utilized ...
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bioRxiv - Biochemistry 2020Quote: ... the protein concentration was determined using the BioRad DC microplate assay protocol (BioRad DC Protein Assay Kit, BioRad, Hercules, California #5000112). Protein Standards and samples were run on NuPAGE 10% Bis-Tris Gels (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... N2a cells were harvested at the same time points for reverse-transcriptase (RT) PCR generation using iScript cDNA Synthesis kit (Bio-Rad), and quantitative PCR (QPCR ...
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bioRxiv - Cancer Biology 2020Quote: RNA was isolated and measured as detailed above and then converted to cDNA using iScript cDNA Synthesis Kit (Bio-Rad #1708891). Quantitative RT-PCR was carried out with TaqMan Gene Expression Assay reagents ...
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bioRxiv - Cancer Biology 2020Quote: ... Revelation was performed using secondary antibodies conjugated to horse-radish peroxidase (GE-Healthcare) diluted 1:2,000 in saturation solution and Opti-4CN™ colorimetric kit (Bio-Rad). ImageJ software was used to measure the relative intensity of protein bands.
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bioRxiv - Cell Biology 2021Quote: ... Reverse transcription of 1 μg of RNA in a total volume of 20 μl was performed with iScript cDNA Synthesis Kit (Bio-Rad) prior to real time PCR (iCycler ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative real time PCR was performed using the iQTM SYBR Green Supermix PCR kit with the iCycler apparatus system (Bio-Rad). GAPDH was used as the invariant housekeeping gene internal control.
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bioRxiv - Physiology 2020Quote: ... We synthesized a total of 500 ng (pituitary) and 1 μg (gonad) of RNA into cDNA using the iScript cDNA synthesis kit (Bio-Rad) in 20 μl reactions according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 0.5 µg of each RNA sample was used in a 10-µL reverse transcription reaction set up using the iScript Select cDNA synthesis kit (Bio-Rad), together with gene-specific reverse primers (Table S2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... We performed cDNA synthesis with 0.5-1 μg of total RNA using the iScript Select cDNA Synthesis Kit (Bio-Rad Laboratories). We performed Real Time quantitative PCR (RT-qPCR ...
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bioRxiv - Systems Biology 2020Quote: ... and used 500 ng - 1 ug RNA to make cDNA using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA). 0.1 of cDNA (i.e ...
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bioRxiv - Plant Biology 2020Quote: ... and the Takyon No ROX SYBR 2X MasterMix Blue dTTP qPCR Kit (both Eurogentech) for quantitative real-time PCR using a CFX96 detection system (Bio-Rad). Transcript data was normalized to UBQ10 ...
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bioRxiv - Plant Biology 2021Quote: ... ∼1 ug of RNA from resulting extraction was used as template in standard cDNA synthesis reaction (BioRad iScript cDNA Synthesis Kit). 200 ng of resulting cDNA was used in qRT-PCR reactions to quantify POL expression levels (PowerUp SYBR Green 5x Master Mix-Thermo) ...
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bioRxiv - Microbiology 2021Quote: ... RNA samples were stored at −80°C until cDNA synthesis using the iScript™ gDNA Clear cDNA Synthesis Kit according to the manufacture’s guidelines (BIO-RAD). A qPCR-based assay was used to detect and quantify PfCSP transcripts (Kefi et al. ...
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bioRxiv - Genomics 2021Quote: ... 30-40 ng of RNA was used to make cDNA using the iScript Adv cDNA Syn Kit (Bio-Rad life Sciences). The RNA was diluted 1:50 in water for ddPCR analysis ...
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bioRxiv - Genetics 2021Quote: ... 0.4 μM or 3.2 μM of SAM-3H3 were used and the reaction was cleaned with either a PCR purification kit or Bio-Spin P30 columns (Bio-rad). The reactions were incubated for 2 hrs at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... Immunoreactive bands were detected using an ECL detection kit (ATTO, EzWestLumi plus, WSE-7120) with a ChemiDoc imaging system (BIO-RAD).
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bioRxiv - Microbiology 2021Quote: ... Additional one-ml samples were used for total protein determination using the Bio-rad Protein Assay kit (Bio-rad, CA, USA). β-galactosidase assays were performed as described previously (38) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... SYBR Green qPCR was performed using a ViiA7 (A&B Biosystems, Waltham, MA) with iTaq Universal SYBR Green One Step Kit (BioRad, #1725151) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... The blots were developed using enhanced chemiluminescence using Cynagen Westar Supernova detection kit and imaged using ChemiDocTM system from Bio-Rad. The blots were then stripped and re-probed for total proteins ...
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bioRxiv - Microbiology 2020Quote: ... All gene transcripts were quantified by quantitative PCR using qScriptTM One-Step qRT-PCR Kit (Quanta Biosciences, #95057-050) on CFX96 real-time PCR system (Bio-Rad). Primer sequences for qPCR were as follow ...
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bioRxiv - Cancer Biology 2020Quote: ... Quantitative real-time PCR (qPCR) was carried out by using SYBR Green Supermix kit with the iCycler QTX detection system (Bio-Rad). Primers are listed in Table EV5 ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration was established using BCA kits (Pierce) and 10µg of protein was loaded into each well of a 4-20% pre-cast gel (BioRad, MiniProtean TGX), flanked with ladder at either end of the gel (PageRuler ...
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bioRxiv - Neuroscience 2022Quote: ... The concentration of 1000 ng of RNA was used for cDNA synthesis using a High Capacity cDNA Reverse Transcription Kit (BioRad, #1708891). To detect the contaminated DNA we used the samples processed without the transcriptase reverse enzyme as negative controls ...
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bioRxiv - Neuroscience 2022Quote: ... we synthetized cDNA using immunoprecipitated samples from Npas1-Cre-RT and RT mice (see above for detailed procedure) using the iScript cDNA synthesis kit (Bio-Rad). cDNA was preamplified using TaqMan Gene Expression Assay system (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... Three to four hundred nanograms of complementary DNA was synthesized using a reverse transcriptase complementary DNA synthesis kit (iScript, Bio-Rad). Changes in mRNA expression were measured using quantitative PCR (Biorad ...
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bioRxiv - Plant Biology 2021Quote: ... and 2 μg of the RNA was employed to synthesize cDNA using the iScript cDNA Synthesis Kit (Bio-Rad, Munich, Germany). Quantitative RT- qPCR analysis was performed on a Bio-Rad iQ5 real-time PCR instrument with the iQ SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: Total RNA (up to 1 µg per reaction) from BMDM was reverse transcribed with iScript cDNA synthesis kit (Bio-Rad, USA). Quantitative PCR was performed using 1 µl of cDNA ...
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bioRxiv - Immunology 2020Quote: ... Measurements and analyses of secreted cytokines from a murine 35-plex kit were performed using a multiplex bead-based technology (Luminex) assay with a Bio-Plex 200 instrument (Bio-Rad) after 2-fold dilution of supernatants.
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bioRxiv - Biochemistry 2021Quote: The details of the protein purification protocol for human plasma sample are described in the Aurum serum mini kit leaflet and a detailed was followed(BioRad, USA). The sample was prepared for 2-D gel electrophoresis following passive rehydration for 16-24 h ...
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bioRxiv - Plant Biology 2021Quote: ... Quality and quantity of RNA samples were assessed using the Bio-Rad Experion analyser and Experion RNA high-sense analysis kit (Bio-Rad). Total RNA from each biological replicate was amplified using the MessageAmp II aRNA amplification kit (Ambion ...
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bioRxiv - Microbiology 2020Quote: ... Washed cells were embedded in 2 % Low melt agarose plugs and cells were lysed (1 hr at 37°C) with lysozyme enzyme (100 μl) using CHEF Bacterial Genomic DNA Plug Kit (Bio-Rad). DNA containing plugs were washed twice with nuclease free water then treated with Proteinase K (Qiagen ...
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bioRxiv - Physiology 2021Quote: ... The supernatant was collected and the protein concentration was measured using a DC protein assay kit (Bio-Rad, Hercules, CA, USA). Equal amounts of protein (5 μg ...
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bioRxiv - Microbiology 2021Quote: ... and 30 ng of RNA from each sample was reverse transcribed using the iScript™ cDNA Synthesis Kit (1708891; Bio-Rad). Real-time PCR was performed using Applied Biosystems™ TaqMan™ Gene Expression Master Mix (4369016 ...
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bioRxiv - Biochemistry 2020Quote: ... The cDNA was prepared in a 10 μl volume containing 350 ng of plant total RNA that was reverse-transcribed with an iScriptTM cDNA Synthesis Kit using modified MMLV-derived reverse transcriptase (Bio-Rad), a blend of oligo-d(T ...
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bioRxiv - Microbiology 2021Quote: ... was used to detect and quantify SARS-CoV-2 RNA using the SARS-CoV-2 Droplet Digital PCR Kit (Bio-Rad), which contains a triplex assay of primers/probes aligned to the CDC markers for SARS-CoV-2 N1 and N2 genes and human RPP30 gene ...
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bioRxiv - Cancer Biology 2021Quote: ... The DNA was quantified on a Bio-Rad T100 96-Well PCR Gradient Thermal Cycler with the SYBR Green kit (Bio-Rad, SsoAdvanced™ Universal SYBR® Green Supermix) ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was quantitated by NanoDrop and 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche) ...
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bioRxiv - Molecular Biology 2022Quote: ... 20-30 μg of total protein was resolved on 8-16% TGX stain-free gels and transferred onto nitrocellulose membrane using the Trans-Blot Turbo RTA Transfer kit and the Trans-Blot Turbo Transfer system set to 7 min transfer protocol (all from Bio-Rad). Stain-free gel and blots were imaged using the Gel Doc EZ imaging system (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... Lysates from the ribopuromycylation assays were loaded into gels prepared using the TGX Stain-Free FastCast Acrylamide Kit (Bio-Rad, 1610183) (Fig ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and then reverse transcription was performed by HiScript III RT SuperMix for qPCR kit (R323, Vazyme, Nanjing, China) by BIO-RAD iCycler(BIO-RAD ...
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bioRxiv - Systems Biology 2022Quote: ... Transfer was done into PVDF membranes using a Trans-Blot Turbo Transfer System and Trans-Blot Turbo RTA Transfer Kit (BioRad, #1704273). After 1 h of blocking in 5% milk ...
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bioRxiv - Neuroscience 2022Quote: One ng of total RNA was used for single-tube reverse transcription (RT) and ddPCR using the One-Step RT-ddPCR Advanced kit (Bio-Rad). Droplets were generated and subsequently analyzed using the QX100 system (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... One microgram of total RNAs was used to generate cDNA using the iScript cDNA synthesis kit (Bio-Rad, Richmond, CA, USA). Real-time PCR was subsequently performed using the iTaq Universal SYBER Green Supermix using the primers listed in Supplemental Table 1 on a CFX96 Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... Yield of RNA yield was quantitated by nano-drop and 1 µg of RNA was use to reverse-transcribed to cDNA using the iScript cDNA synthesis kit (BIORAD; #1708891) (Roche) ...
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bioRxiv - Immunology 2022Quote: ... was converted to cDNA with the C1000 Touch Thermal Cycler using the iScript cDNA Synthesis Kit according to the manufacturer’s protocol (Bio-Rad, 1708890), with the following modifications ...