Labshake search
Citations for Bio-Rad :
4551 - 4600 of 5608 citations for Mouse Leucine Rich Pentatricopeptide Repeat Containing LRPPRC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... Total RNA was extracted and qRT-PCR was performed using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA) and 1000 ng of total RNA according to the manufacturer’s instructions as previously described (64) ...
-
bioRxiv - Microbiology 2020Quote: ... Real-time PCR was performed using using the SYBR Green kit (iTaq Universal SYBR Green Supermix, BIO-RAD, 1725124) on a BIO-RAD CFX384 ...
-
bioRxiv - Microbiology 2020Quote: ... Immunoreactive bands were detected using an ECL detection kit (EzWestLumi plus, ATTO) with a ChemiDoc imaging system (Bio-Rad).
-
bioRxiv - Cancer Biology 2020Quote: ... About 500-1000ng RNA was used as a template for cDNA synthesis using a cDNA synthesis kit from Biorad. The cDNA was further diluted to 1:10 with nuclease-free water ...
-
bioRxiv - Cell Biology 2021Quote: ... signals were obtained with peroxidase-conjugated secondary antibodies (Cell Signalling Technology) and revealed with Clarity Western ECL kit (Biorad) and a ChemiDoc ™ XRS Imaging system (BioRad).
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... kit and BioRad chemiluminiscence machine scanner.The densitometry analysis of immunoblot images was performed using quantity One Software(Bio-Rad). Densitometry values were normalzed to the loading control(Gapdh or Actin ...
-
bioRxiv - Animal Behavior and Cognition 2022Quote: ... The total RNA extracted was reverse transcribed using the iScript™ cDNA Synthesis kit (Bio-Rad Laboratories, CA, USA) with a thermal profile ...
-
bioRxiv - Neuroscience 2022Quote: ... 300-400 ng of cDNA from VP was synthesized using the reverse transcriptase iScript cDNA synthesis kit (Bio-Rad). mRNA expression changes were measured using qRT- PCR with PerfeCTa SYBR Green FastMix (Quanta) ...
-
bioRxiv - Cell Biology 2022Quote: ... 1 μg of each RNA sample was reverse transcribed to cDNA using the iScript cDNA synthesis kit (Bio-Rad). Quantitative PCR was performed on cDNA samples using the iQ SYBRGreen Supermix (Bio-Rad ...
-
bioRxiv - Cell Biology 2022Quote: ... 200 ng of the extracted RNA was reverse-transcribed to cDNA using the iScript cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... cDNA was prepared from 10 to 50 ng RNA according to instructions of iScript™ cDNA Synthesis Kit (BioRad). Qiagen RNeasy® Plus mini kits (Qiagen ...
-
bioRxiv - Neuroscience 2020Quote: ... and quantified using the ddPCR Library Quantification Kit for Illumina TruSeq in a QX200 Droplet Digital PCR system (BioRad). Details information on the NGS protocol are described in the SI.
-
bioRxiv - Cell Biology 2021Quote: ... Real-time PCR reactions were set up using the iTaq Universal Sybr Green One-Step RT-PCR kit (Biorad) according to manufacturer’s directions ...
-
bioRxiv - Neuroscience 2020Quote: ... Protein concentration was determined using a Bio-Rad DC™ protein assay kit (Bio-Rad, Marnes-La-Coquette, France). Proteins (30 µg ...
-
bioRxiv - Plant Biology 2021Quote: ... RNA was extracted as described above and cDNA was synthesized using an iScript™ cDNA Synthesis Kit (Bio-Rad). PCR was performed using Bio-Rad CFX96 Real-Time PCR systems ...
-
bioRxiv - Plant Biology 2021Quote: ... The iScript™ cDNA synthesis kit and iTaq universal SYBR green PCR master mix were obtained from Bio-Rad Laboratories ...
-
bioRxiv - Microbiology 2020Quote: ... were detected by staining membranes with anti-mouse IFN-γ biotin (1μg/ml) (R46A2) followed by streptavidin-Alkaline Phosphatase (1μg/ml) and development with AP conjugate substrate kit (BioRad, UK).
-
bioRxiv - Microbiology 2021Quote: ... Purified RNA (500 μg) was used to synthesize cDNA using the iScript™ cDNA Synthesis Kit (Bio-Rad, USA). Pre Developed Taqman® (Thermo Fisher ...
-
bioRxiv - Cancer Biology 2021Quote: ... according to the manufacturer’s instructions and cDNA synthesis was performed using the iScript Advanced cDNA synthesis kit (Bio-Rad) using a mix containing 8 μl sample lysate ...
-
bioRxiv - Microbiology 2021Quote: ... Viral RNA was then quantified by qRT-PCR using the iTaq™ Universal Probes One-Step Kit (BIO-RAD) according to manufacturer’s protocol using a forward primer (5’-CCGCTGCCCAACACAAG-3’) ...
-
bioRxiv - Microbiology 2021Quote: ... and amplicons were generated via qRT-PCR using iTaq™ Universal SYBR® Green One-Step Kit (BIO-RAD) according to manufacture protocol ...
-
bioRxiv - Neuroscience 2020Quote: ... cDNA was synthesized from RNA using Biogen iScript kit and qPCR was performed using either Sybr Green iTaq (BioRad) or TaqMan (Life Technologies ...
-
bioRxiv - Cancer Biology 2022Quote: ... an iScript reverse transcription kit was used to convert one microgram of RNA to cDNA (Bio-Rad, Hercules, CA). No reverse transcriptase controls were included ...
-
bioRxiv - Cell Biology 2022Quote: ... 1000 ng RNA was reverse-transcribed into cDNA using the iScript™ cDNA Synthesis Kit (Bio-RAD, CA, USA). cDNA was diluted 1:5 in PCR-grade dH2O and 1.5 µl of the cDNA-mix was used per 20 µl real-time PCR reaction with 0.25 µM of each primer and 2 x FastStart Essential DNA Green Master Mix (Roche) ...
-
bioRxiv - Plant Biology 2019Quote: cDNA was synthetised from 1μg of total RNA using the iscript Reverse Transcriptase Kit (Bio-rad Cat no 1708891) in 20 μl of final volume ...
-
bioRxiv - Neuroscience 2019Quote: ... The concentration of solubilized protein was determined with a Bio-Rad Protein Assay kit (Bio-Rad, Hercules, CA USA). The retinal lysate with loading buffer was boiled for 3 min prior to loading onto an 8% sodium dodecyl sulfate (SDS)-polyacrylamide gel with the same amount of protein (5ug ...
-
bioRxiv - Physiology 2019Quote: ... sonicated for 20 seconds and protein concentration was determined with the commercial kit (DC™ Protein Assay; Bio-Rad). Fifteen µg of proteins were reduced with modified Laemmli buffer (40 mM Tris pH 6.8 ...
-
bioRxiv - Immunology 2019Quote: ... Cells were then stained for inflammasome activation using a caspase-1 FAM-YVAD-FMK FLICA probe kit (Bio-Rad). Cells were then processed for analysis by flow cytometry.
-
bioRxiv - Synthetic Biology 2019Quote: ... Protein concentrations in the resuspended samples were quantified using a DC Protein Assay Kit (Bio-Rad Laboratories, Hercules, CA). After quantification ...
-
bioRxiv - Developmental Biology 2019Quote: ... Reverse transcription was performed on 1 μg of purified RNA using the iScript Reverse Transcription Supermix kit (Bio-Rad). The cDNA was then added to SsoFast (Bio-Rad ...
-
bioRxiv - Plant Biology 2019Quote: ... RNA integrity was assessed by microfluidic capillary gel electrophoresis using the ExperionTM RNA HighSens Analysis Kit (#7007105, Bio-Rad Laboratories ...
-
bioRxiv - Microbiology 2019Quote: ... 1µg of purified RNA was used to make cDNA using the iScript™ cDNA synthesis kit (Bio-Rad, USA) using the manufacturer’s recommended protocol ...
-
bioRxiv - Genetics 2020Quote: ... according to the manufacturer’s instructions and reverse transcribed to cDNA using iScript cDNA synthesis kit (Bio-Rad, Hercules, CA). Quantitative PCR was performed on a Bio-Rad CFX96 real-time system using SsoFast Evagreen mix (Bio-Rad ...
-
bioRxiv - Developmental Biology 2021Quote: ... 4 hpf (n=40) or 48 hpf (n=25) embryos using the Aurum Total RNA Mini Kit (Bio-Rad). cDNA was then prepared with the RevertAid reverse transcriptase (Thermo Fisher) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Reverse transcription of 1 μg RNA/20μl was performed with the iScript™ cDNA Synthesis Kit (1708890, Bio-Rad). qPCR was performed with iTaq™ Universal SYBR® Green Supermix (1725120 ...
-
bioRxiv - Neuroscience 2021Quote: ... We used 1µg of RNA to produces cDNA using the iScript cDNA synthesis kit from Bio-Rad (Hercules, CA). Primers used for RT-PCR are listed in Table 1.
-
bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from plants using the Aurum Total RNA kit according to the manufacturer’s instructions (Bio-Rad). First-strand cDNA was synthesized in a 10-μl volume containing 350 ng of plant total RNA that was reverse transcribed employing an iScript cDNA Synthesis Kit (Bio-Rad ...
-
bioRxiv - Genomics 2020Quote: Genomic DNA for optical mapping was extracted from agarose-embedded specimens using the CHEF Genomic Plug DNA kit (BioRad) in order to minimise fragmentation ...
-
bioRxiv - Plant Biology 2021Quote: ... 1μg of total RNA were used for cDNA synthesis using iScript™ cDNA Synthesis Kit (Bio-rad Laboratories, India). For quantitative real-time PCR (qRT-PCR) ...
-
bioRxiv - Microbiology 2020Quote: ... The protein concentration of all fractions was quantified using the Quick Start™ Bradford Protein Assay Kit (Bio-Rad Laboratories ...
-
bioRxiv - Microbiology 2020Quote: cDNA was synthesized from 0.5 μg of total RNA using the iScript Advanced cDNA synthesis kit (Bio-Rad; 1725037) following the protocol 25°C for 5 min ...
-
bioRxiv - Cell Biology 2020Quote: ... and loaded onto 10% SDS-PAGE gel (TGX™ Fast Cast™ Acrylamide Kit 10%, BioRad, California, United States). Proteins were transferred onto a nitrocellulose membrane using the Trans-Blot® Turbo™ semi-liquid rapid transfer system (BioRad) ...
-
bioRxiv - Developmental Biology 2020Quote: ... cDNA was synthesized from total RNA (0.1 – 1.0 µg) with iScript Advanced cDNA Synthesis Kit (BioRad, Hercules, CA, USA). QRT-PCR was performed using PowerUp SYBR Green Master Mix reagent (Applied Biosystems ...
-
bioRxiv - Immunology 2019Quote: ... The extracted RNA was amplified by iTaq universal SYBR Green one-step PCR kit (Bio-Rad, Hercules, CA, USA) and 10μM primers (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2019Quote: The qPCR mixture of 10 μl was prepared using the iQ™ SYBR® Green PCR Kit (Bio-Rad): 5 μl of 2x iQ™ SYBR® Green Master Mix ...
-
bioRxiv - Cell Biology 2019Quote: ... RNA (1 μg) was reverse transcribed in 20 μl reactions using the BioRad iScript cDNA Synthesis Kit (BioRad Laboratories) with 1:1 mixture of oligo-dT and random hexamers for 30 min at 42°C ...
-
bioRxiv - Physiology 2019Quote: Total RNA was extracted and reverse-transcribed according to the manufacturer’s protocol (iScript™ cDNA Synthesis Kit, BIO-RAD). cDNA was loaded in duplicate and qPCRs were performed using the BIO-RAD CFX System (BIO-RAD ...
-
bioRxiv - Genomics 2021Quote: The single nucleus ATAC-seq experiment was performed using the SureCell ATAC-Seq Library Prep Kit (Bio-Rad, 17004620) following the manufacturer’s protocol for the OMNI-ATAC version (45) ...
-
CCG•CGG interruptions in high penetrance SCA8 families increase RAN translation and protein toxicitybioRxiv - Genetics 2021Quote: ... The protein lysate concentration was quantified using Pierce BCA Protein Assay Kit (ThermoScientific) and10μg of soluble protein lysates were separated on a 4-12% Bis-Tris gel (BioRad) and transferred to a nitrocellulose membrane ...
-
bioRxiv - Genetics 2020Quote: ... digital droplet PCR (ddPCR) was performed using the One-step RT-ddPCR advanced kit for probes (Bio-Rad, #1864022). The final 20 μL reaction mix contained ...