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Citations for Bio-Rad :
4451 - 4500 of 7138 citations for 7 Chloro 4 hydroxy 1 8 naphthyridine 3 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: Cell lysates were prepared using RIPA buffer (see lysis and click chemistry methods below) and run on SDS Tris-Glycine gels (Precast 4 - 20% Bio-Rad, or hand-casted 20%). Gels were transferred to low-background PVDF membranes using the BioRad TransBlot Turbo system and blocked with 5% milk powder in TBS + 0.1% Tween 20 (TBST ...
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bioRxiv - Microbiology 2020Quote: Twenty-fold concentrated viral stocks or purified rgp120 were separated by SDS-PAGE under reducing conditions using a 4-15% Mini-PROTEAN TGX precast gel (Bio-Rad Laboratory Inc. Hercules, CA), and then transferred onto a polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2022Quote: ... and were heated to 95°C for 5 min before being separated by SDS-PAGE with Criterion Precast Gels (Bio-Rad, 4–15% Tris/Glycine) and then transferred onto 0.2 µm nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were clarified at 13,200 rpm for 15 min at 4°C prior to quantification by Lowry assay (Bio-Rad cat # 5000113 and cat # 5000114). Whole cell lysates were loaded into 4-20% Criterion TGX Precast 18 well gels (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... Sample preparations were run on a 15% separating gel with a 4% stacking gel under reducing conditions using Tris-glycine running buffer (mini-PROTEAN system, Bio-Rad Laboratories, Hercules, CA, USA). Silver staining using sodium thiosulphate sensitisation and silver nitrate was performed according to established methods (22).
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bioRxiv - Physiology 2020Quote: ... Amplification reactions and disassociation curves of 4-fold dilution series of cDNA were carried out on a CFX384 machine (Bio-Rad, Bio-Rad, Mississauga, ON), for determination of primer efficiencies ...
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bioRxiv - Physiology 2020Quote: ... and an equal amount of total protein (30 μg) per sample was subjected to standard SDS-PAGE using Mini-PROTEAN® TGX™ 4-20% 12-wells gels (Bio-Rad, Hercules, CA). Gels were electroblotted onto PVDF membranes (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... resolved by SDS-PAGE (4-15% acrylamide, 150V) and transferred to PVDF membranes using the Trans-Blot® Turbo™ Transfer System (Bio-Rad Laboratories, CA). Membranes were incubated for 1 hr in 5% milk (in TBS-T ...
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bioRxiv - Genetics 2020Quote: ... and ligated to MseI and EcoRI adaptors (Supplementary Table 4) at 37° C for 2 h in a T100™ Thermal cycler (Bio-Rad Laboratories, Hercules, CA). Success of the digestion/ligation reaction was confirmed on 1.5% of agarose gel electrophoresis ...
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bioRxiv - Developmental Biology 2020Quote: ... was performed using the primer pairs listed in Table 4 and iTaq Universal SYBR Green Master Mix on a CFX384™ Real-Time PCR detection system (Bio-Rad Laboratories, Hercules, CA). Fold changes in gene expression were calculated by the ΔΔCt method using the weighted average of Ct values for the housekeeping genes Pol2ra ...
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bioRxiv - Neuroscience 2021Quote: ... or 40 μg (splenocytes of WT and Pik3cd−/− mice) of lysate were loaded onto 4-15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad Laboratories B.V.). Transfer was performed onto Immobilon®-P PVDF Membranes (Merck KGaA) ...
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bioRxiv - Neuroscience 2022Quote: For each lane, ∼20 μg protein were run on a 4-12Bis-Tris Criterion XT Precast Gel (#3450123, Bio-Rad (Bio-Rad, Hercules, CA, USA) next to the Precision Plus Protein Dual Color Standard (catalog#161-0374 ...
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bioRxiv - Immunology 2024Quote: ... were resolved by SDS-PAGE using 4-20% Mini-PROTEAN® TGX Stain-Free Precast™ Gels gels and transferred onto Trans-Blot® Turbo™ membranes (Bio-Rad). Membranes were blocked with 5% non-fat milk in TBS-T for 1 hour at room temperature and probed with primary antibodies against (Influenza A virus NS1 ...
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bioRxiv - Neuroscience 2023Quote: ... from cells or brain tissue were separated on SDS polyacrylamide gels (4–15% Mini-PROTEAN® TGX Stain-Free™ gels, Bio-Rad, Hercules, USA) and transferred onto a membrane (nitrocellulose or PVDF ...
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bioRxiv - Cancer Biology 2023Quote: Proteins from cell extracts were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) on 4-20% gradient gels (Bio-Rad #4561095; Hercules, CA, USA) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2023Quote: ... 10-20 μg of total protein lysate was loaded into a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad, cat. no. 4561095). Gels were transferred into PVDF membranes and blocked for 1 hour at room temperature in 5% BSA ...
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bioRxiv - Neuroscience 2023Quote: ... 10-20 µg of total protein lysate was loaded into a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad, cat. no. 4561095). Gels were transferred into PVDF membranes and blocked for 1 hour at room temperature in 5% BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... 15 micrograms of total protein were separated using stain-free 4–15% gradient Tris-glycine SDS-polyacrylamide gels (Mini PROTEAN® TGX Stain-Free™ Gels, Bio-Rad Laboratories, CA) and transferred onto PVDF membranes (Amersham Hybond ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 20 μg of proteins were analyzed in a polyacrylamide gel (4–20% Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad, Marnes-la-Coquette, France) and separated by electrophoresis ...
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bioRxiv - Biochemistry 2024Quote: ... 20 to 30 µg of total protein was separated by SDS-PAGE on a pre-cast 4–15% Criterion™ Protein Gel (Bio-Rad Laboratories, Cat#5671084) followed by an overnight transfer and western blot analysis.
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bioRxiv - Neuroscience 2024Quote: ... 50 µL of media was reduced and denatured prior to gel electrophoresis on a 4-15% tris-glycine gel (#4561084, Bio-Rad Laboratories, Hercules, CA, USA) for 2 hours @ 175 mV ...
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bioRxiv - Molecular Biology 2024Quote: ... were resolved on a 4-15% or 10% acrylamide gel and transferred onto a PVDF membrane (Invitrogen iBlot 2 or Bio-Rad Trans-Blot Turbo system). Membranes were blocked with 5% milk (standard protein detection ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal amounts of protein (25 µg) were denatured 1:1 in 2X Laemmli buffer (BIO-RAD) at 95°C for 10 minutes then held on ice ...
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bioRxiv - Pathology 2021Quote: ... Protein samples were first diluted 1:1 with Laemmli sample buffer (Bio-Rad, Hemel Hempstead, UK) containing 5% β-mercaptoethanol (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... followed by secondary antibodies (1:4000 donkey anti-goat, Abcam; 1:4000 goat anti-mouse, Biorad). Immunoblots were developed using SuperSignal West Pico PLUS Chemiluminescent Substrate (ThermoFisher).
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bioRxiv - Microbiology 2021Quote: ... 20 uL cell suspension was diluted 1:1 with 0.4% Trypan Blue solution (Bio-Rad #1450021) and cells were counted by hemocytometer (RPE-1 cells ...
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bioRxiv - Neuroscience 2021Quote: ... Each compartment of each sample was diluted 1:1 in Laemlli Sample Buffer (BioRad, Hercules, CA) mixed with 350mM DTT (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... The following antibodies and dilutions were used: F4/80 1:100 (C1:A3-1, Bio-RAD), αSMA 1:100 (AS-29553 ...
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bioRxiv - Microbiology 2022Quote: ... 100 μl fresh urine and several dilutions (1/10; 1/100) were plated on UriSelect4 (Biorad) and KFS (Biolife italiana ...
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bioRxiv - Plant Biology 2023Quote: ... 0.3% (w/v) CHAPS containing protease inhibitors (1 tablet Biorad/10ml ; 1/100 v/v PMSF). Phosphatase inhibitors were also added in L3 and L4 experiments ...
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bioRxiv - Physiology 2022Quote: ... monoclonal rat anti-F4/80 antibody (dilution 1:200) (clone Cl:A3-1, MCA497G, Biorad Laboratories, Germany) After multiple washes ...
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bioRxiv - Pathology 2024Quote: ... Protein samples were first diluted 1:1 with Laemmli sample buffer (Bio-Rad, Hemel Hempstead, UK) containing 5% β-mercaptoethanol (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... TGN46 at 1:1000 (Bio-Rad), for two hours at RT ...
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bioRxiv - Neuroscience 2020Quote: ... BrdU (Rat; 1:500; Bio-Rad). Alexa Fluor 488- ...
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bioRxiv - Neuroscience 2021Quote: ... anti-CD68 (1:500, Bio-Rad), and anti-Iba1 (1:500 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 1 software (Bio-Rad, Hercules, USA).
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bioRxiv - Developmental Biology 2019Quote: ... F4/80 (1:1000, MCA497R, Biorad), MHC II (1:500 ...
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bioRxiv - Neuroscience 2019Quote: ... anti-CD11b (1:400, BioRad, MCA74G), anti-F8/40 (1:100 ...
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bioRxiv - Neuroscience 2019Quote: ... anti-CD68 (1:400, BioRad, MCA1957), anti-CD11b (1:400 ...
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bioRxiv - Neuroscience 2019Quote: ... anti-CD13 (1:50, BioRad, MCA2183), anti-CD68 (1:400 ...
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bioRxiv - Developmental Biology 2019Quote: ... BrdU (Bio-Rad MCA2060, 1:500), Ki67 (Abcam ab16667 ...
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bioRxiv - Immunology 2021Quote: ... NP (BioRad Cat#MCA400, 1:1000), and anti-β-actin (Sigma Aldrich Cat #A2228-100UL ...
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bioRxiv - Bioengineering 2021Quote: ... CD68 (1:100, MCA341R; Bio-Rad), and CD163 (1:400 ...
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bioRxiv - Bioengineering 2020Quote: ... mouse CD68 (Biorad, 1:200, MCA341GA), mouse CD45R (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... or anti-mouse (1:5000, BioRad). Stained membrains were developed with SuperSignal West Pico chemo luminescent substrate ...
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bioRxiv - Neuroscience 2021Quote: ... anti-CD68 (1:100; Biorad; MCA1957), anti-MPO (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... or Collagen-1 (1310001, Bio-Rad). Cells were then washed 3 times with PBS-T ...
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bioRxiv - Cancer Biology 2021Quote: ... β-Actin (Bio-Rad 12004163, 1:10,000) was used as a loading control ...
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bioRxiv - Cell Biology 2022Quote: ... anti-NBS1 (BioRad, VMA00403, 1:1000), anti-MDC1 (Novus Biologicals ...
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bioRxiv - Microbiology 2022Quote: ... mouse anti-LAMP-1 (MCA2315GA; BioRad), rabbit anti-VPS11 (PA5-21854 ...