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Citations for Bio-Rad :
401 - 450 of 523 citations for Recombinant Human Integrin Alpha 8 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Diabetes-associated markers and cytokine levels in the plasma were assessed using the Bio-Plex Pro Mouse Diabetes 8-Plex Assay (Cat# 171F7001M; Bio-Rad, Carlsbad, California) and the Bio-Plex Pro Mouse Cytokine 23-Plex Assay (Cat# M60009RDPD ...
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bioRxiv - Microbiology 2020Quote: ... The precipitated proteins were then dissolved in 6 M urea/50 mM ammonium bicarbonate buffer (pH 8) for protein quantitation using DC™ protein assay (Bio-Rad Laboratories) and trypsin digestion.
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bioRxiv - Cell Biology 2020Quote: ... Total protein (5–20 μg) was resolved onto 8–12% SDS-PAGE and blotted onto 0.2 μm pore size PVDF membranes (1620177, Bio-Rad, Hercules, CA, USA). Primary antibodies were incubated overnight at 4°C after blocking membranes for 1h ...
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bioRxiv - Microbiology 2021Quote: All melt-MAMA PCRs were performed on a CFX96 Real-Time PCR Detection System using white hard shell 96-well PCR plates or 0.2 mL 8-tube PCR strips (Bio-Rad, Gladesville, NSW, Australia), and PCR results examined using the CFX Maestro v4.1.2433.1219 software (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... 40 μg of ΔORF3-E mNG RNA and 20 μg of N-gene RNA were electroporated into 8×106 Vero-ORF3-E cells using the Gene Pulser XCell electroporation system (Bio-Rad, Hercules, CA) at a setting of 270V and 950 μF with a single pulse ...
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bioRxiv - Immunology 2020Quote: ... 30-50µg of protein per lane alongside a prestained molecular weight protein marker was separated on an 8% SDS PAGE gel prepared from SDS-PAGE kit (Beyotime, China) and electrotransferred to immunoblot PVDF membrane (BIO-RAD, CA, USA) for protein blotting ...
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bioRxiv - Molecular Biology 2022Quote: ... chromatin samples were dissolved in SDS-containing loading buffer and the electrophoresis was carried out in 8-16% mini-protean TGX Bio-Rad gels (Cat. # 4561105, Bio-Rad, Hercules, CA) gradient acrylamide gels as described (Laemmli ...
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bioRxiv - Immunology 2022Quote: ... Equal amount of total protein (25 μg/well) was resolved by electrophoresis on 8% Tris-Acrylamide gels and transferred onto 0.2 μm nitrocellulose membranes (Bio-Rad Laboratories, CA, US). Membranes were blocked for 1 h at room temperature in 5% skim milk diluted in Tris-buffered saline supplemented with 0.1% Tween 20 (TBST) ...
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bioRxiv - Microbiology 2023Quote: Serum samples collected from mice were tested in duplicate in a multiplex immunoassay using magnetic beads (Mouse Th1-Th2 8-plex Bio-Rad, Hercules, CA) using the Luminex MAGPIX® system) ...
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bioRxiv - Biochemistry 2023Quote: ... plates were stained with primuline (0.05% in acetone/water, 8/2; v/v) and photographed under long-wave UV light (Biorad ChemiDoc XRS Imaging System).
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bioRxiv - Microbiology 2023Quote: ... for the selected clinical isolates using the indicated concentrations of antibiotics via an alamar blue reduction assay completed as described but without shaking.8 After 3 days of incubation with the alamar blue reagent (BioRad, Hercules, CA, USA), the MIC was identified as the lowest concentration of drug that inhibited the reagent color change as determined visually.
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bioRxiv - Cancer Biology 2024Quote: ... Serum hormones were quantified on a Luminex MAGPIX platform using the Bio-Plex Pro Mouse Diabetes 8-Plex and Adiponectin Assays (Bio-Rad, Hercules, CA) and the IGF-1 mouse Luminex Discovery Assay (R&D Systems ...
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bioRxiv - Immunology 2021Quote: ... specific for the various human chemokine receptor genes and IQ™ SYBR green reagent (# 170882, BIO-RAD) on CFX96 real-time PCR detection system (BIO-RAD) ...
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Tracing production instability in a clonally-derived CHO cell line using single cell transcriptomicsbioRxiv - Cell Biology 2021Quote: ... the light chain by the anti-human kappa light chain antibody (1:2,500; Biorad, cat.no. STAR 127) and GAPDH by the anti-GAPDH monoclonal antibody (1:10,000 ...
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bioRxiv - Microbiology 2021Quote: ... an assay detecting the human ribonuclease P/MRP subunit p30 RPP30 gene (Bio-Rad Assay ID dHsaCP2500350) was used as reference (probe ...
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bioRxiv - Cell Biology 2022Quote: One segment of each explanted human IPF lung was filled with 1.5% Low Melt Agarose (Bio-Rad) at 37°C and allowed to cool on ice for 30 mins ...
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bioRxiv - Cancer Biology 2021Quote: ... For the quantification of metabolic hormones Bio-Plex Pro Human Diabetes 10-plex Assay (BioRad, USA; 171A7001M) was used ...
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bioRxiv - Immunology 2019Quote: ... cells were incubated at 370C for a further 24 h with human oxLDL (#5685-3557, Bio-Rad) at a concentration of 0 µg/ml and 25µg/ml ...
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bioRxiv - Cell Biology 2019Quote: One segment of each explanted human IPF lung was filled with 1.5% Low Melt Agarose (Bio-Rad) at 37°C and allowed to cool on ice for 30 min ...
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bioRxiv - Immunology 2021Quote: ... IL-18 and IL-6 concentrations were measured using the Bio-Plex Pro Human Cytokine Assay (BioRad).
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bioRxiv - Immunology 2022Quote: ... Cytokines and chemokines were measured using the Bio-Plex Pro™ Human Assay (Bio-Rad, Hercules, USA): cytokine screening panel plus ICAM-1 and VCAM-1 (12007283 ...
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bioRxiv - Immunology 2023Quote: ... Human AAVS constructs were transfected into CHO cells using the Gene Pulser MXcell Electroporation System (Bio-Rad). Transfected cells were selected for with 10µg/mL puromycin or 1mg/mL neomycin ...
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bioRxiv - Immunology 2023Quote: ... or sensitized with 1 mg/mL chimeric human IgE anti-NP antibody (MCA333S, BIO-RAD, Hercules, CA), or in combination for 24 hours ...
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bioRxiv - Pathology 2024Quote: ... and cytokines were detected using the Bio-Plex Pro Human Cytokine 17-plex Assay (Bio-Rad, #M5000031YV), including IL-1β ...
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bioRxiv - Immunology 2024Quote: ... 1:1200 diluted mouse anti-human HLA-E monoclonal primary antibody (clone MEM-E/02, Bio-Rad) and as secondary antibody goat anti-mouse polyclonal antibody conjugated with horse radish peroxidase (HRP ...
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bioRxiv - Cancer Biology 2024Quote: ... diluted 1:4 and analyzed using Bio-Plex ProTM Human Chemokine Panel 40-plex Assay (Bio-rad). Acquisitions and analyses were performed on a Bio-Plex 200 system with Manager 6.1 Software (Bio-rad) ...
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bioRxiv - Immunology 2024Quote: ... Approximately 600 RUs of anti-human IgG (Cytiva) were immobilized on a GLC Sensor Chip (Bio-Rad) using amine-coupling ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... while insulin levels were assayed using the quantitative Bio-Plex Pro™ Mouse Diabetes 8-Plex immunoassay (#171F7001M; Bio-Rad Laboratories, Hercules, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 10 μg of transcribed RNA were electroporated into 8×106 Vero cells using the Gene Pulser Xcell™ Electroporation Systems (Bio-rad, California, USA) under as described previously41 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Twenty microlitres of the mastermix + template DNA were pipetted into the very bottom of the central row of (8) wells of the Droplet Generator DG8™ Cartridge (Bio-Rad 186-4008). Sixty-five microlitres of droplet generation oil for probes (BioRad 186-3005 ...
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Biallelic loss-of-function OBSCN variants predispose individuals to severe, recurrent rhabdomyolysisbioRxiv - Genetics 2021Quote: Frozen muscle biopsies were homogenised in modified Laemmli sample buffer as described previously.37 Samples were run in Bio-Rad Criterion 3–8 % tris-acetate gradient gels (Bio-Rad Laboratories, CA, USA) at room temperature ...
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bioRxiv - Immunology 2022Quote: ... cell debris was removed by centrifugation and clear supernatants were diluted 10-fold and subjected to anion exchange chromatography on gravity fed columns using AG-1 9 8 resin (formate form, 100–200 mesh size, Bio-Rad Laboratories, Hercules, CA). Fractions containing inositol mono ...
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bioRxiv - Biochemistry 2020Quote: ... resolved on the 8% SDS PAGE and transferred to nitrocellulose membrane (Trans-Blot Turbo Midi Nitrocellulose Transfer Pack, Bio-Rad, 0.2 µm pore size) with the use of a Trans-Blot Turbo Transfer Starter System (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... IL-6, TNF-α, IL-8, IP-10, MCP-1, MIP-1α, MIP-1β, IL-1RA, G-CSF and Eotaxin; Bio-Rad Laboratories Inc.) following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... following which they were subjected to separation via sodium dodecyl sulfate-polyacrylamide gel electrophoresis using 8% or 10% gels and the Mini-Protein II apparatus (Bio-Rad, CA, United States). Protein bands were electroblotted onto the polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2022Quote: ... cell viability was determined using the Cell Counting Kit-8 (Dojindo, Kumamoto, Japan) by measuring the absorbance at 450 nm using an iMarkTM microplate absorbance reader (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Neuroscience 2023Quote: ... Forty µg denatured protein per well was resolved by 8-12% SDS- PAGE homemade gels in parallel with multi-colored molecular weight marker (Bio-Rad Laboratories, Cat.No.1610395). Gels were run at 100V for 1-2 h and transferred overnight at 8- 12V ...
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bioRxiv - Immunology 2024Quote: ... Cell lysates were separated on an 8-12% SDS-polyacrylamide gel and blotted onto a polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Hercules, CA, USA), The membrane was incubated overnight at 4°C with primary Abs ...
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bioRxiv - Microbiology 2020Quote: ... Analyses were performed with the Extended Bio-Plex Pro Human Cytokine 48-Plex Screening Panel (Biorad, Munich, Germany) as described previously 72 and using a 2-laser reader allowing simultaneous quantification of cytokines and chemokines ...
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bioRxiv - Cancer Biology 2019Quote: ... Collected sera were subjected to ELISA by capturing with human anti-rituximab idiotype antibody (HCA186, Bio-Rad Laboratories), detected with rat-anti-rituximab-HRP antibody (MCA2260P ...
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bioRxiv - Bioengineering 2020Quote: ... Isolated EVs were stained with PE-labelled anti-human TNFR1 (Bio-Rad, cat #MCA1340PE, clone H398, lot 0407) and APC-labelled anti-mouse gp130 antibodies (R&D Systems ...
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bioRxiv - Immunology 2022Quote: ... We used mouse anti-human HLA-ABC (W6/32) conjugated to Alexa Fluor® 647 (MCA81A647, Bio-Rad), which is known to also react with MHC-I of African Green monkey from which Vero E6 cells are derived(48 ...
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bioRxiv - Immunology 2019Quote: ... fatty acid free BSA Efflux was measured after a 4-hour incubation period with 0µg/ml and 50µg/ml of human HDL (#5685-2004, BioRad). Supernatants were collected and the cells lysed with 1% (w/v ...
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bioRxiv - Immunology 2020Quote: ... The binding was detected by an HRP-conjugated anti-human IgG (Fc) CH2 Domain antibody (Bio-Rad MCA647P) used at a 1:5,000 dilution at RT for 1 h and developed as described above.
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bioRxiv - Neuroscience 2023Quote: ... and Bio-Plex Pro Human Inflammation Panel 1 (37-plex) (Cat. No. 171AL001M) from Bio-Rad (Hercules, CA).
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bioRxiv - Molecular Biology 2019Quote: ... All reactions were quenched with 6X SDS loading buffer and fractionated on an 8% tris-glycine SDS-PAGE gel along with a protein ladder (BioRad, Precision Plus Dual Color Standard). A photograph of the dried protein gel was overlaid with the scanned phosphorimage of the gel to estimate the molecular weights of bands on the phosphorimage.
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bioRxiv - Plant Biology 2022Quote: ... Fifty microliters of crude protein extract per sample were resolved on an 8% polyacrylamide gel and transferred to a polyvinylidene difluoride membrane (PVDF-Immun-Blot®, Bio-Rad, Hercules, CA, USA) as described previously (Qi and Mi ...
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bioRxiv - Microbiology 2022Quote: ... samples were separated on 8-12% SDS-PAGE gels and transferred to nitrocellulose membranes using semi-dry transfer (BioRad Trans-Blot Semi-Dry Transfer Cell). Membranes were blocked in 5% non-fat milk in Tris-buffered saline with 0.1% Tween-20 (TBST ...
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bioRxiv - Cell Biology 2022Quote: Cytokine and growth factor secretion was measured with the Bio-Plex Pro Human Cytokine Screening Panel (Bio-Rad #M500KCAF0Y), a 27-plex assay ...
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bioRxiv - Neuroscience 2021Quote: ... The isolated placental CD34+ single cell suspension was incubated with human CD34- phycoerythrin (PE) (Bio-Rad; dilution 1:25), human CD45-FITC (BioLegend ...