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Citations for Bio-Rad :
401 - 450 of 580 citations for IL 6 Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and growth factor protein expression was quantified using the Bio-Plex Pro Rat Cytokine 23-plex assay (Bio-Rad Laboratories, Inc). Analytes included in the assay were ...
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bioRxiv - Pathology 2021Quote: ... The sections were then incubated in blocking buffer (10% goat serum; 1 hour) followed by incubation with either rat anti-F4/80 antibody (Bio-Rad) diluted 1:500 or rat anti-CD68 antibody (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... All slides were subsequently blocked with 4% BSA and 1% Triton X-100 in PBS and incubated with rat anti-CD68 (1:500; Bio-Rad) and mouse-anti-GFP conjugated to Alexa Fluor 488 (1:250 ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were incubated for one hour either with a 1:5 dilution of Rat anti Human CD3:Alexa Fluor®647 (0.05 mg/mL) (clone CD3-12; Bio-Rad) or with undiluted CD8 (AM22 ...
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bioRxiv - Cancer Biology 2023Quote: ... Expression levels of 85 genes were assessed using the PrimePCR Antibacterial Response (SAB Target List) R384 rat plate (Catalog #10047065; Bio-Rad) using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) ...
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bioRxiv - Immunology 2024Quote: ... tissue sections were blocked in 10% normal goat serum for 1 h at room temperature and incubated in rat anti-mouse CD4 (1:200; MCA2691; Bio-Rad) or CD8a (Biolegend ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR products were assessed using 6% polyacrylamide gel (made with 29:1 Acrylamide/Bis Solution, Bio-Rad) electrophoresis ...
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bioRxiv - Genomics 2020Quote: ... The reaction was quenched with 2µl of 10mM EDTA and desalted with Microbio-spin 6 column (BioRad) to remove unincorporated nucleotides ...
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bioRxiv - Biochemistry 2020Quote: ... The thiol-reduced protein was exchanged into anaerobic 0.5x TNG buffer using Microbiospin 6 columns (Bio-Rad). The protein concentration was determined by the Bradford assay (ThermoFisher scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Unconjugated dye was removed by passing the protein through Micro Bio-spin P-6 column (Bio-rad) equilibrated in GF150 buffer (25 mM HEPES (pH 7.4) ...
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bioRxiv - Microbiology 2020Quote: ... separated on 6% acrylamide 8M urea gels and transferred to Zeta Probe GT membranes (Bio-Rad laboratories) by electroblotting ...
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bioRxiv - Plant Biology 2019Quote: ... 5- to 6-week-old expanded leaves of Arabidopsis plants were bombarded with 1nm gold particles (BioRad) coated with pB7WG2.0-GFP and pB7WG2.0-RFPER ...
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bioRxiv - Neuroscience 2019Quote: ... Protein lysates (50µg) were loaded on a 6% SDS-PAGE gel and transferred to nitrocellulose membrane (BioRad) using a wet electroblotting system (BioRad) ...
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bioRxiv - Cancer Biology 2019Quote: ... washed with TBST (6 × 5 min) and developed using Clarity Western ECL Substrate (Bio-Rad, California, US). Images were captured with ChemiDoc XRS+ system (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... The DNA preparation involves coating 10 μl of .6 μm Gold microcarriers (1652262 Bio-Rad, Hercules, CA) with 1μg of purified DNA by gently vortexing DNA ...
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bioRxiv - Plant Biology 2022Quote: ... cumana KAI2d1-6 genes from parental lines and segregants using iProof High-Fidelity DNA Polymerase (BioRad #172530). The PCR products were then sequenced via MiSeq ...
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bioRxiv - Molecular Biology 2022Quote: ... The reaction mixture was desalted by twice passing through Micro Bio-spin 6 chromatography columns (Bio-Rad).
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bioRxiv - Molecular Biology 2022Quote: ... The reaction mixture was desalted by twice passing through Micro Bio-spin 6 chromatography columns (Bio-Rad).
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bioRxiv - Plant Biology 2023Quote: ... the treated protein was cleaned up using a Micro Bio-SpinTM P-6 Gel Column (BIO-RAD), and then they were further subjected to 5 mM sodium (meta ...
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bioRxiv - Molecular Biology 2023Quote: ... then resuspended in 600 µl TNM100 buffer and transferred into Micro Bio-Spin 6 columns (Bio-Rad). The elution from IgG Sepharose beads was achieved by incubation with 30 µl homemade GST-tagged TEV protease for two hours on a shaking table at 16 °C.
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bioRxiv - Microbiology 2023Quote: ... diluted 1:1,000 in 6% milk in PBS-T followed by α-mouse IgG HRP (Bio-Rad) diluted 1:5,000 in 6% milk in PBS-T ...
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bioRxiv - Cancer Biology 2023Quote: 6-well culture plates were coated with 1 ml of 0.6 % Low Melt Agarose (Bio-Rad; 1613111). Cells were diluted at a density of 1.0 x 104 cells/ml in media containing 0.3% Low Melt Agarose and seeded 1 ml/ well ...
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bioRxiv - Developmental Biology 2023Quote: ... and monitoring of mCherry expression after 6 and 22 h (ZOE fluorescent cell imager, Bio-Rad, 1450031).
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bioRxiv - Developmental Biology 2020Quote: ... followed by Super Signal@ West Femto chemiluminescent substrate (Pierce, Rockford, IL, USA) and the products were recovered by Laser 4000 chemiluminescent system (Bio-Rad, CA, USA). Finally ...
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bioRxiv - Genetics 2021Quote: ... 1 mM dithiothreitol and 0.5% Tween 20] for 24 hours and its concentration was quantified by Bradford assay (Bio-Rad, Des Plaines, IL).
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bioRxiv - Neuroscience 2022Quote: ... then placed in 4% normal goat serum in 0.01M PBS blocking solution for 60 minutes with agitation followed by incubation with rat anti-CD68 (1:500; BioRad, cat no. MCA1957GA) and rabbit anti-MBP (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies used in this study were anti-myelin basic protein (MBP, rat, monoclonal, clone 12, Bio-Rad, cat# MCA409S, 1:1000), anti-Neurofilament 200 (NF200 ...
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bioRxiv - Neuroscience 2022Quote: Cytokine levels were assessed using Bio-Rad BioPlex assays in both plasma and PFC homogenates (Th1/Th2 rat cytokines Bio-Rad, #171k1002M), according to manufacturer’s protocols ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were blocked using 5% BSA in PBS and incubated overnight at 4 °C with 1 or an appropriate combination of up to 3 of the following antibodies: rat anti-CD8 (1:500, catalog no. MCA609G; Bio-Rad Laboratories), rat anti-CD4 (1:1,000 ...
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bioRxiv - Molecular Biology 2019Quote: ... 30 μL egg extract was applied to and eluted from a Micro Bio-Spin 6 column (Bio-Rad) equilibrated in ELB150 ...
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bioRxiv - Plant Biology 2021Quote: ... At least 4-6 technical replicate RT-qPCR reactions were performed using iTAQ with ROX and SYBR (BioRad), and 20μL reactions were prepared as per the manufacturer recommendations ...
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bioRxiv - Biochemistry 2022Quote: ... and 0.032 % (w/v) SM was applied to a centrifugal device (Micro Bio-Spin 6, Bio-Rad, Hercules) to exchange the buffer to a MS buffer containing 200 mM ammonium acetate (pH 7.4) ...
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bioRxiv - Biophysics 2022Quote: ... and 1 mM EDTA in 99.9% deuterium oxide using a spin desalting column (Micro Bio-Spin 6, BioRad). 15N-HSQC spectra were measured every 40 min for 106 hours at 25 °C on Bruker 600 MHz NMR spectrometer ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was purified by loading quenched samples onto Micro Bio-spin Columns with Bio-Gel P-6 (BioRad), followed by ethanol precipitation ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by 6 washes in 100 µL of PBST in Bio-Spin columns (catalog number 7326204, Bio-Rad). The bound immunocomplexes were eluted via 0.1 M glycine ...
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bioRxiv - Biochemistry 2021Quote: KirBac3.1 protein was buffer-exchanged to 200 mM ammonium acetate (pH=8.0) with 0.5% C8E4 (2×CMC) using a Biospin-6 column (BioRad). The protein sample was loaded into a gold coated needle prepared in-house and analysed on a modified Q-Exactive mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... then washed 6 x 5 minutes with TBS-T before developing with Clarity Western ECL Substrate (Bio-rad).
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bioRxiv - Biochemistry 2022Quote: ... solutions containing purified CytbX were buffer-exchanged into 0.2 M ammonium acetate (pH 8.0) containing 2x CMC of Lauryldimethylamine oxide (LDAO) using Biospin 6 columns (Biorad). 2-3 µL of 5 µM protein solution was introduced directly into Q-Exactive UHMR mass spectrometer (ThermoFisher ...
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bioRxiv - Biophysics 2022Quote: ... then 6 L of RO water with Ca2+ chelating beads (BIO-RAD Chelex 100 Resin Cat#142-1253), and finally 6 L of RO water alone ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was then cleaned 2-times on Micro Bio-Spin™ P-6 gel Columns (BioRad, #732-6221) where buffer was exchanged with phase separation buffer ...
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bioRxiv - Microbiology 2023Quote: ... separated on 6% acrylamide 8 M urea gels and transferred to Zeta Probe GT membranes (Bio-Rad laboratories) by electroblotting ...
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bioRxiv - Genetics 2021Quote: ... followed by purification with RNeasy minicolumns (Qiagen, μg total RNA was reverse-transcribed using an iScript cDNA synthesis kit (Bio-Rad, Des Plaines, IL). Assay of RNA via quantitative PCR (qPCR ...
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bioRxiv - Neuroscience 2023Quote: ... 04404-84, Nacalai tesque), anti-APC (CC1, 1/100, OP80, Merk, Darmstadt, Germany) and rat anti-MBP (1/100, MCA409S, Bio-Rad, California, USA) in antibody dilution buffer (1% normal goat serum ...
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bioRxiv - Immunology 2023Quote: ... The expression of immune system markers were mostly studied with inmunohistochemistry using the following antibodies: mouse anti rat CD68 (dilution 1:300 BIORAD, ref. MCA 341R) for monocytes/macrophages ...
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bioRxiv - Cell Biology 2022Quote: ... Excess dye was removed by two consecutive buffer exchanges through Micro Bio-Spin P-6 desalting columns (Bio-Rad). Protein concentration and labeling efficiency were estimated using a NanoDrop Microvolume Spectrophotometer.
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bioRxiv - Genomics 2019Quote: Binding reactions were incubated at room temperature for 30 min and loaded onto a 6% polyacrylamide 0.5 × Criterion precast TBE gel (Biorad). After sample electrophoresis and transfer to a nylon membrane ...
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bioRxiv - Biochemistry 2020Quote: ... 0.14 M NaCl (PBS) followed by removal of the reductant using a Micro Bio-Spin 6 column (Bio-Rad). Columns were washed and pre-equilibrated with 20 mM phosphate buffer pH 7.4 containing 0.1 mM diethylenetriamine-penta-acetic acid (DTPA ...
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bioRxiv - Biochemistry 2021Quote: ... individual proteins were buffer exchanged into 200 mM ammonium acetate pH 8.0 either by SEC or using Biospin-6 (BioRad) columns and introduced directly into the mass spectrometer using gold-coated capillary needles (prepared in-house) ...
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bioRxiv - Biochemistry 2020Quote: ... His-tagged protein was detected using a mouse anti-6×His IgG conjugated to horseradish peroxidase (MCA1396P; Bio-Rad), and the blot was developed using Pierce™ ECL Western Blotting Substrate (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2020Quote: ... The annealed doubly nicked DNA duplex was then buffer-exchanged in Hepes 20 mM pH 7.5 with a BioSpin 6 column (BioRad).